• Korean Journal of Plant Resources
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• v.29 no.3
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• pp.297-304
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• 2016

The seed of safflower (Carthamus tinctorius L) has been reported to suppress human cancer cell proliferation. However, the mechanisms by which safflower seed inhibits cancer cell proliferation have remained nuclear. In this study, the inhibitory effect of the safflower seed (SS) on the proliferation of human colorectal cancer cells and the potential mechanism of action were examined. SS inhibited markedly the proliferation of human colorectal cancer cells (HCT116, SW480, LoVo and HT-29). In addition, SS suppressed the proliferation of human breast cancer cells (MDA-MB-231 and MCF-7). SS treatment decreased cyclin D1 protein level in human colorectal cancer cells and breast cancer cells. But, SS-mediated downregulated mRNA level of cyclin D1 was not observed. Inhibition of proteasomal degradation by MG132 attenuated cyclin D1 downregulation by SS and the half-life of cyclin D1 was decreased in SS-treated cells. In addition, SS increased cyclin D1 phosphorylation at threonine-286 and a point mutation of threonine-286 to alanine attenuated SS-mediated cyclin D1 degradation. Inhibition of ERK1/2 by PD98059 suppressed cyclin D1 phosphorylation and downregulation of cyclin D1 by SS. In conclusion, SS has anti-proliferative activity by inducing cyclin D1 proteasomal degradation through ERK1/2-dependent threonine-286 phosphorylation of cyclin D1. These findings suggest that possibly its extract could be used for treating colorectal cancer.

• Natural Product Sciences
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• v.29 no.1
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• pp.31-37
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• 2023

In this study, we investigated the efficacy of Celtis choseniana Nakai (C. choseniana) as complementary herbal medicine to ameliorate androgenic alopecia (AGA). The effects of C. choseniana on AGA were evaluated using testosterone propionate-induced AGA mouse model and dihydrotestosterone-treated human hair follicle dermal papilla cells. In vivo, C. choseniana treatment deactivated androgen signaling by reducing the concentration of serum dihydrotestosterone level and expressions of 5α-reductase 2 and androgen receptor. Next, C. choseniana treatment increased the hair regrowth rate. Histological studies demonstrated that C. choseniana induced the anagen phase in testosterone propionate-induced AGA mouse model. Cellular proliferation was promoted by C. choseniana treatment via increasing the expression of proliferation factors, such as proliferating cell nuclear antigen and cyclin D1. Furthermore, C. choseniana treatment increased the expression of proteins related to the Wnt/β-catenin signaling pathway. In addition, dickkopf-1, a Wnt inhibitor, was downregulated with C. choseniana treatment. Likewise, C. choseniana treatment promoted cellular proliferation in vitro. This study demonstrated the inhibitory effect of C. choseniana on androgen-induced AGA. Moreover, C. choseniana induced activation of Wnt/β-catenin signaling, resulting in prolonged anagen and cellular proliferation. Therefore, we suggest that C. choseniana can be used as a therapeutic agent to alleviate AGA.

• Journal of the Korean Professional Engineers Association
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• v.42 no.3
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• pp.35-40
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• 2009

After the yield of North Korea conducted underground the first nuclear test in 2006, which was less than a kiloton, it launched a vehicle on this April 9 again, which is believed to be a Taepo-dong 2 missile, defying international warnings that the move would violate U.N. resolutions, from Musudan-ni, North Korea. Its development and proliferation of ballistic missile and nuclear bomb technology pose a threat to the northeast Asian region and to international peace and security. In the other launching the missile, a newspaper reported that "While the launch was dubbed a failure by the U.S., it was an improvement over the North Koreans' July 2006 test of the Taepodong-2, said Green of the Center for Strategic and International Studies. "It was definitely better than last time, but it's still not an operational system," Green said.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2003.11a
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• pp.172-179
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• 2003

Various type of tritium wastes can be produced from nuclear fuel cycle process satisfying non-proliferation, CANDU reactors, and nuclear industry. Activities of tritium processing in the world were surveyed to develope the processing technologies of tritium wastes. The tritium wastes were classified into gas phase, liquid phase, and organic phase. And the treatment techniques for the tritium wastes are analyzed. Development of tritium processing technologies is essential to finding public acceptance of radioactive wastes and forming a solid foundation to foster the growth of nuclear industry in Korea.

• Proceedings of the Korea Information Processing Society Conference
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• 2020.05a
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• pp.188-190
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• 2020

최근 4차 산업혁명과 더불어 센서 네트워크와 같은 최신 무선통신 기술들의 기반시설 적용을 위한 연구들이 활발하게 이루어지고 있다. 원자력시설 또한, 보안 및 비상대응 시스템에 무선통신을 적용하기 위한 연구들이 진행되고 있으며, 미국과 UAE의 경우 이미 원자력시설에 무선통신을 적용하여 사용하고 있다. 그러나 무선통신의 경우, 물리적인 네트워크 접근 경로가 존재하지 않기 때문에 통신 경로에 대한 접근통제가 불가능하며 광범위한 지역에 네트워크를 설치하는 경우 중계 단말 수량의 증가로 인한 접근통제 취약점이 발생할 가능성이 있다. 이와 같은 무선통신의 특성 때문에 원자력시설의 필수디지털자산에 무선 네트워크를 적용 시 현재의 통신 경로 접근통제 등의 유선 통신을 기준으로 작성된 접근통제 규제기준으로는 무선통신에 대한 접근통제를 이행하기에는 부족함이 있다. 이에 본 논문에서는 무선 네트워크 접근통제를 위한 규제 기준 개선안을 제시한다.

• International Journal of Advanced Culture Technology
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• v.12 no.2
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• pp.309-317
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• 2024

The conflict between Iran and Israel has been a major source of instability and security threats in the Middle East. This study analyzes the historical background and modern aspects of the Israel-Iran conflict, examining its root causes, nuclear issues, proxy wars, cyber warfare, economic sanctions, and the role of the United States. The research employs qualitative methods, including literature review and case analysis, using various sources such as academic papers, government reports, and media articles. The findings suggest that the conflict is deeply rooted in historical, religious, and political factors, and has escalated since the 1979 Iranian Revolution. The study highlights the need for dialogue, negotiation, and international mediation to resolve the conflict and maintain regional stability. The Israel-Iran conflict also provides valuable lessons for addressing the North Korean nuclear issue, emphasizing the importance of dialogue, international cooperation, and confidence-building measures.

• Korean Journal of Veterinary Service
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• v.31 no.4
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• pp.457-463
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• 2008

The sex steroid hormone estradiol-$17{\beta}(E_2)$ mediate their biological effects on development, differentiation and maintenance of reproductive tract and other target tissue through gene regulation by nuclear steroid receptors. Although the importance of $E_2$ in many physiological process has been reported, but little is known about the effects of $E_2$ on primary cultured chicken hepatocyte. therefore, in the present study, we have examined the effect of $E_2$ on cell proliferation and it's related signal cascades. $E_2$ increase $[^3H]$-thymidine incorporation in time-(${\leq}8hr$) and dose-($10^{-10}M$)dependent manner and treatment of $E_2$ increased the phosphorylation of p44/43 MAPKs(p44/42 mitogen-activated protein kinase) and JNK(c-Jun N-terminal kinase) in a time dependent manner. In addition, PD98059(p44/42 blocker, $10^{-5}M$), SP600125(JNK blocker, $10^{-6}M$) blocked the estrogen-induced increase in $[^3H]$-thymidine incorporation. In conclusion, $E_2$ stimulates the proliferation of primary cultured chicken hepatocytes and this action is mediated by p44/42 MAPKs and JNK signal transduction pathway.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.31-31
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• 2018

Benign prostatic hyperplasia (BPH), which is the most common disorder in elderly men, involves androgenic hormone imbalance with chronic inflammation that causes imbalance between cell apoptosis and cell proliferation. As the root cause of the BPH remains unclear and synthetic drugs for treatment of BPH have undesirable side effects, the development of effective alternative medicines has been considered. Chinese Skullcap has been considered natural remedy to treat pyrexia, micturition disorder and inflammation. Although skullcap has effective properties on various diseases, the effects and molecular mechanism of Skullcap on BPH are not fully understood. Therefore, in this study, we evaluated the efficacy of Chinese Skullcap root extract (SRE) in testosterone-induced BPH rats. Compared with the untreated group, the SRE treatment group suppressed pathological alterations, such as prostate growth and increase in serum dihydrotestosterone and $5{\alpha}$-reductase levels. Furthermore, SRE significantly decreased the expression of androgen receptor and proliferating cell nuclear antigen. SRE also restored Bax/Bcl-2 balance. These effect of SRE was more prevalent than commercial $5{\alpha}$-reductase inhibitor, finasteride. Taken together, we propose that SRE suppresses abnormal androgen events in prostate tissue and inhibits the development of BPH by targeting inflammation- and apoptosis-related markers. These finding strengthens that SRE could be used as plant-based $5{\alpha}$-reductase inhibitory alternative.

• BMB Reports
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• v.47 no.10
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• pp.581-586
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• 2014

Epidermal growth factor (EGF) is known to play key roles in skin regeneration and wound-healing. Here, we demonstrate that Pep2-YAC, a tripeptide covering residues 29-31 in the B loop of EGF, promotes the proliferation of HaCaT keratinocytes with activity comparable to EGF. The treatment of HaCaT cells with Pep2-YAC induced phosphorylation, internalization, and degradation of EGFR and organization of signaling complexes, which consist of Grb2, Gab1, SHP2, and PI3K. In addition, it stimulated the phosphorylation of ERK1/2 at Thr 202/Tyr 204 and of Akt1 at Ser 473 and the nuclear translocation of EGFR, STAT3, c-Jun, and c-Fos. These results suggest that Pep2-YAC may be useful as a therapeutic agent for skin regeneration and wound-healing as an EGFR agonist.

• Applied Microscopy
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• v.45 no.2
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• pp.80-88
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• 2015

The structural change and distribution of mitochondrial enzyme (ATPase, cytochrome-c-oxidase), cell proliferation (proliferating cell nuclear antigen, PCNA), cell death (caspase-3) and cell growth factor (fibroblast growth factor 8, FGF-8) in the Sprague-Dawley rat bile duct during Clonorchis sinensis infection was investigated. Experimental groups were divided into C. sinensis infection, superinfection and reinfection of C. sinensis after 'praziquantel' treatment group. As a result, C. sinensis infected rat bile ducts showed the features of chronic clonorchiasis, i.e., connective tissue thickening, ductal fibrosis and epithelial tissue dilatation. PCNA for cell proliferation increased in the infection group, and decreased after praziquantel treatment. Caspase-3 was distributed in reinfection group only. FGF-8 was distributed in the rat bile duct after praziquantel treatment but not distributed in infection and reinfection group. Overall, C. sinensis infection causes physical and chemical irritations and then brings on the abnormalities of intracellular energy metabolism and cellular growth factors, which hinders bile duct tissue from functioning properly, and resultingly, fibrosis occurs and epithelial cells dilated abnormally. More intense infection makes tissue fibrosis chronical and activates apoptosis factors.