• 한국자기공명학회논문지
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• 제28권1호
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• pp.1-5
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• 2024

IscU, the iron-sulfur (Fe-S) cluster scaffold protein, is an essential protein for biogenesis of Fe-S clusters. Previous studies showed that IscU manifests a metamorphic structural feature; at least two structural states, namely the structured state (S-state) and the disordered state (D-state), interconverting in a physiological condition, was observed. Moreover, subsequent studies demonstrated that the metamorphic flexibility of IscU is important for its Fe-S cluster assembly activity as well as for an efficient interaction with various partner proteins. Although solution nuclear magnetic resonance (NMR) spectroscopy has been a useful tool to investigate this protein, the detailed molecular mechanism that sustains the structural heterogeneity of IscU is still unclear. To tackle this issue, we applied a high-pressure NMR (HP-NMR) technique to the IscU variant, IscU(I8K), which shows an increased population of the S-state. We found that the equilibrium between the S- and D-state was significantly perturbed by pressure application, and the specific regions of IscU exhibited more sensitivity to pressure than the other regions. Our results provide novel insights to appreciate the dynamic behaviors of IscU and the related versatile functionality.

• Journal of Animal Science and Technology
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• 제64권2호
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• pp.247-261
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• 2022

Ketosis is associated with high milk yield during lactating or insufficient feed intake in lactating dairy cows. However, few studies have been conducted on the metabolomics of ketosis in Korean lactating dairy cows. The present study aimed to investigate the serum and urine metabolites profiling of lactating dairy cows through proton nuclear magnetic resonance (¹H-NMR) spectroscopy and comparing those between healthy (CON) and subclinical ketosis (SCK) groups. Six lactating dairy cows were categorized into CON and SCK groups. All experimental Holstein cows were fed total mixed ration. Serum and urine samples were collected from the jugular vein of the neck and by hand sweeping the perineum, respectively. The metabolites in the serum and urine were determined using ¹H-NMR spectroscopy. Identification and quantification of metabolites was performed by Chenomx NMR Suite 8.4 software. Metabolites statistical analysis was performed by Metaboanalyst version 5.0 program. In the serum, the acetoacetate level was significantly (p < 0.05) higher in the SCK group than in the CON group, and whereas acetate, galactose and pyruvate levels tended to be higher. CON group had significantly (p < 0.05) higher levels of 5-aminolevulinate and betaine. Indole-3-acetate, theophylline, p-cresol, 3-hydroxymandelate, gentisate, N-acetylglucosamine, N-nitrosodimethylamine, xanthine and pyridoxine levels were significantly (p < 0.05) higher in the urine of the SCK group than that in the CON group, which had higher levels of homogentisate, ribose, gluconate, ethylene glycol, maltose, 3-methyl-2-oxovalerate and glycocholate. Some significantly (p < 0.05) different metabolites in the serum and urine were associated with ketosis diseases, inflammation, energy balance and body weight. This study will be contributed useful a future ketosis metabolomics studies in Korea.

• Journal of Biosystems Engineering
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• 제24권6호
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• pp.531-538
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• 1999

비파괴 품질 평가 기술인 핵 자기공명 분광법과 자기공명영상을 이용하여 소고기의 내부 구조를 분석하였다. 이완 상수 T$_1$과 T$_2$가 육류의 내부 구조와 관계가 있음을 보였다. 근육부분의 면적 비율이 증가할수록 T$_1$이 증가했다. 지방 부분의 T$_2$는 배 내부 부분의 T$_2$와 비슷한 값을 보였다. 자기공명 영상을 이용하여 육류의 원하는 부위의 T$_2$를 측정할 수 있는 방법을 구하였다. 근육 부분의 T$_2$가 길었으며 지방 부분의T$_2$가 짧았다. 부위별로 최적의 신호를 얻을 수 있는 자기공명영상 인자 TR과 TE를 구하였다. 자기공명영상을 이용하여 근육, 지방 그리고 뼈 성분에 따른 육류의 품질을 비파괴적으로 평가할 수 있는 가능성을 보여주었다.

• 한국자기공명학회논문지
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• 제20권1호
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• pp.17-21
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• 2016

Periostin, a component of extracellular matrix (ECM) protein, is produced and secreted by the fibroblasts that are involved in chronic allergic inflammation diseases and various types of human cancers. Periostin protein is composed of multiple domains including four FAS1 domains which play important roles in cell adhesion and tumor metastasis by interacting with integrins. In spite of their important biological role, the structural information of periosin FAS1 domains was not revealed yet. Recently we systemically prepared various constructs of the FAS1 domains and tried to express them in E. coli. Of them, only single FAS1-II and -IV domains were highly soluble. Circular dichroism (CD) and nuclear magnetic resonance (NMR) studies revealed that the FAS1-IV domain might be suitable for three-dimensional structure determination using NMR spectroscopy.

• 한국자기공명학회논문지
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• 제6권1호
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• pp.38-44
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• 2002

60% Pt on Vulcan XC-72 with similar Pt sizes to fuel cell grade Pt black was investigated by $\^$13/C nuclear magnetic resonance spectroscopy (NMR), cyclic voltammery (CV), transmission electron microscopy (TEM). Experiments were carried out on electrochemically cleaned samples as well as as-received. The TEM and CV results showed that the average particle sizes were changed by cleaning. However, the chemical shift （$\delta$$\_$G/） of $\^$13/C of $\^$13/CO absorbed on Pt surfaces did not show any appreciable variation with particle size change as did in Pt black. These results indicate that a combination of different analytic techniques is essential to understand the properties of the metal particle catalysts and that the presence of carbon black support strongly influences the NMR data, probably through metal-support interaction.

• 한국자기공명학회논문지
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• 제13권1호
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• pp.56-63
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• 2009

SWIRM domain, a core domain of SRG3 is well conserved in SW13, RSC8, and MOIRA family proteins. To understand structural basis for cellular functions of the SWIRM domain, we have initiated biochemical and structural studies on SWIRM domain and mutants using gelfiltration chromatography, circular dichroism and NMR spectroscopy. The structural properties of the mutant SWIRM domains (K34A and M75A) have been characterized, showing that the structures of both wild-type and mutant proteins are a-helical conformation. The data conclude that mutations at interaction sites of its binding partner protein do not affect its secondary and tertiary structure.

• 한국자기공명학회논문지
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• 제19권3호
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• pp.132-136
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• 2015

Nuclear magnetic resonance (NMR) spectroscopy, owing to its ability to provide atomic level information on molecular structure, dynamics and interaction, has become one of the most powerful methods in early drug discovery where hit finding and hit-to-lead generation are mainly pursued. In recent years, drug discovery programs originating from the fragment-based drug discovery (FBDD) strategies have been widely incorporated into academia and industry in which a wide variety of NMR methods become an indispensable arsenal to elucidate the binding of small molecules onto bimolecular targets. In this review, I briefly describe FBDD and introduce NMR methods mainly used in FBDD campaigns of my company. In addition, quality control of fragment library and practical NMR methods in industrial aspect are discussed shortly.

• 한국자기공명학회논문지
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• 제26권4호
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• pp.59-65
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• 2022

Benzo[a]pyrene (BaP), one of the polycyclic aromatic hydrocarbons (PAHs), is an endocrine disruptor and carcinogenic. This study was conducted to investigate the metabolic changes of zebrafish short-term exposure to BaP using nuclear magnetic resonance (NMR) spectroscopy. In our results, the multivariate analysis showed that the metabolic responses were differed according to the exposure concentration. Also, it was observed that exposure to high concentration of BaP (162 ㎍/L and 1620 ㎍/L) increased the levels of creatine, histidine, and inosine in zebrafish, which means high concentration of BaP exposure affected the energy metabolism and immune function in zebrafish.

• 한국자기공명학회논문지
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• 제27권1호
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• pp.1-4
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• 2023

Transthyretin (TTR) is an indispensable transporter protein of thyroxine and a retinol molecule in humans. TTR has a stable homo-tetrameric structure in its native state, while upon dissociation into monomers, it becomes aggregation-prone and can form an amyloid fibril. Although the amyloidogenic propensity of TTR has been known and investigated since the late 1990s, the structural information regarding TTR's amyloidogenic species is still elusive. Here, we employed high-pressure nuclear magnetic resonance (HP-NMR) approaches on the monomeric variant of TTR (TTR[F87M/L110M]; M-TTR) and observed that it experiences a two-step transition in response to the pressurized condition. Our study demonstrated that M-TTR in an ambient condition has heterogeneous structural features, which is likely related to the amyloidogenic propensity of TTR.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.92-95
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• 2000