• Proceedings of the KSME Conference
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• 2004.04a
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• pp.96-101
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• 2004

Cast austenitic stainless steel is used for several components, such as primary coolant piping, elbow, pump casing and valve bodies in light water reactors. These components are subject to thermal embrittlement at the reactor operating temperature. The objective of this study is to summarize the method of estimating ferrite content, Charpy impact energy and J-R curve and to evaluate the thermal embrittlement of the cast austenitic stainless steel piping used in the domestic nuclear power plants. The result of evaluation, two domestic nuclear power plants used CF-8M and CF-8A material has adequate fracture toughness after saturation.

• Korean Journal of Animal Reproduction
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• v.26 no.4
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• pp.353-360
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• 2002

Although successful pronuclear formation and apposition were seen in porcine oocytes following mouse sperm injection, little is known on the morphology of male and female pronuclei following sperm injection. The objective of this study is to describe the ultrastructure of porcine zygote following murine sperm injection in relation to the chronology of pronuclear S phase. At 40h ~ 44h following in vitro maturation, Cumulus cells were removed in TCM-HEPES with 0.1% hyaluronidase. Then, spermatozoa was injected into the cytoplasm of oocytes. After. injection, all oocytes were transferred to NCSU23 medium and cultured at 39$^{\circ}C$ under 5% $CO_2$ in air. Oocytes were fixed in 2% glutaraldehyde in Dulbeccos phosphate-buffered saline and observed by Transmission Electron Microscopy. Nuclear precursor bodies were observed in each pronucleus. A cluster of large and small granules was attached in the nucleolus precursor body. After the apposition of male and female chromatin, chromatin condensation was observed throughout the nucleoplasm and nucleolus precursor bodies and condensed chromatin in contact with clusters of small and large granules and the nuclear envelope were found in apposed pronuclear regions. These results suggest that non-species specific nuclear cytoplasmic interactions take place during pronuclear formation and apposition following sperm injection.

• Nuclear Engineering and Technology
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• v.54 no.10
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• pp.3631-3640
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• 2022

Uranium mill tailing ponds (UMTPs) are risk source of debris flow and a critical source of environmental U and Rn pollution. The technology of microbial induced calcium carbonate precipitation (MICP) has been extensively studied on reinforcement of UMTs, while little attention has been paid to the effects of MICP on U & Rn release, especially when incorporation of metakaolin and bacillus subtilis (MBS). In this study, the reinforcement and U & Rn immobilization role of MBS -MICP solidification in different grouting cycle for uranium mill tailings (UMTs) was comprehensively investigated. The results showed that under the action of about 166.7 g/L metakaolin and ~50% bacillus subtilis, the solidification cycle of MICP was shortened by 50%, the solidified bodies became brittle, and the axial stress increased by up to 7.9%, and U immobilization rates and Rn exhalation rates decrease by 12.6% and 0.8%, respectively. Therefore, the incorporation of MBS can enhance the triaxial compressive strength and improve the immobilization capacity of U and Rn of the UMTs bodies solidified during MICP, due to the reduction of pore volume and surface area, the formation of more crystals general gypsum and gismondine, as well as the enhancing of coprecipitation and encapsulation capacity.

• Clinical and Experimental Reproductive Medicine
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• v.50 no.3
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• pp.177-184
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• 2023

Objective: Reconstructed oocytes after polar body genome transfer constitute a potential therapeutic option for patients with a history of embryo fragmentation and advanced maternal age. However, the rescue of genetic material from the first polar body (PB1) through introduction into the donor cytoplasm is not yet ready for clinical application. Methods: Eighty-five oocytes were obtained following in vitro maturation (IVM) and divided into two groups: PB1 nuclear transfer (PB1NT; n=54) and control (n=31). Following enucleation and PB1 genomic transfer, PB1 fusion was assessed. Subsequently, all fused oocytes underwent intracytoplasmic sperm injection (ICSI) and were cultured in an incubator under a time-lapse monitoring system to evaluate fertilization, embryonic morphokinetic parameters, and cleavage patterns. Results: Following enucleation and fusion, 77.14% of oocytes survived, and 92.59% of polar bodies (PBs) fused. However, the normal fertilization rate was lower in the PB1NT group than in the control group (56.41% vs. 92%, p=0.002). No significant differences were observed in embryo kinetics between the groups, but a significant difference was detected in embryo developmental arrest after the four-cell stage, along with abnormal cleavage division in the PB1NT group. This was followed by significant between-group differences in the implantation potential rate and euploidy status. Most embryos in the PB1NT group had at least one abnormal cleavage division (93.3%, p=0.001). Conclusion: Fresh PB1NT oocytes successfully produced normal zygotes following PB fusion and ICSI in IVM oocytes. However, this was accompanied by low efficiency in developing into cleavage embryos, along with an increase in abnormal cleavage patterns.

• Journal of the Ergonomics Society of Korea
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• v.26 no.4
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• pp.121-125
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• 2007

The objective of this study is to develop short-term prevention measures for minimizing possible human error in nuclear power facilities. To accomplish this objective, a group of subject matter experts (SMEs) were formed, which is consisting of those from regulatory bodies, academia, industries and research institutes. Prevention measures were established for urgent execution in nuclear power facilities on a short-term basis. This study suggests short-term measures for reducing human error on three different areas; (1) strengthening worker management, (2) enhancing workplace environments and working methods, and (3) improving the technologies regulating human factors. Under the leadership of the Ministry of Science and Technology, these short-term measures will be pursued and implemented systematically by utility and regulatory agencies. The details of prevention measures are presented and discussed.

• Applied Microscopy
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• v.9 no.1
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• pp.35-56
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• 1979

To investigate ultrastructural characteristics of cancer cells of the nervous system, 25 cases; i.e. astrocytoma(9), oligodendroglioma(1), medulloblastoma(1), meningioma(5), pinealoma(2) and pituitary adenomas(7). The common findings were marked irregularity of nuclear membrane with pronounced infoldings, clumping of heterochromatin along inner nuclear membrane, enlargement of nucleolus, and frequent observations of nuclear bodies and nuclear inclusions. But these findings are also the signs that can be observed in hyperactive cells. Thus, ultrastructural characteristics of cancerous nucleus are the great variability of nuclear size, shape and composition. but none of them appear to be specific. Among cytoplasmic organelles, massive fibrils are characteristic of astrocytoma and meningiomas, cytoplasmic protofibrils such as glial process and microvesicles in oligodendroglioma, secretory granules are characteristic in pituitary adenomas, and fine filamentous fibrils and desmosomes are characteristic of fibroblastic type of meningioma. Intercellular relationships and cell membrane specialization are important features in the differential diagnosis of various undifferentiated tumors. The frequent resolution of difficult diagnosis problems by electron microscopy outweighs the disadvantages of this technique, such as the expense and time required.

• Nuclear Engineering and Technology
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• v.55 no.6
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• pp.1959-1965
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• 2023

The objective of this study was the establishment of clearance method that can ensure radiological safety and reasonably minimize radioactive waste when demolishing contaminated buildings at KRR-1&2. By reviewing Korean and international laws related to decommissioning, the method for clearance of contaminated buildings presented in this study is to first decontaminate the building and then conduct a radiological safety assessment, such as measuring residual radioactivity, to determine whether the radiation dose criteria for clearance are satisfied. The measurement results meet the radiation dose criteria, the contaminated buildings are regarded as clearance and can be converted into the general buildings. The demolition of the cleared buildings is carried out using conventional demolition methods. The waste generated during the demolition is classified as general construction waste and is disposed of according to relevant laws. The proposed method significantly optimized the number of samples analyzed and reduced the time and cost associated with the decommissioning. The established method will be applied to the ongoing decommissioning of contaminated buildings at KRR-1&2, and its application will be verified by regulatory bodies. The study suggests that this method could be used for the decommissioning of contaminated buildings at other Korean nuclear facilities in the future.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2002.09a
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• pp.199-201
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• 2002

Cancer therapy using high-energy $^{12}$ C ions is successfully under way at HIMAC, Japan. An alternative beam to $^{12}$ C is $^{11}$ C ions. The merit of $^{11}$ C over $^{12}$ C is its capability for monitoring spatial distribution of the irradiated $^{11}$ C by observing the $\beta$$^{+}$ decay with a good position resolution. One of the several problems to be solved before its use for therapy is the amount of nuclear interaction that deteriorates the dose concentration owing to the Bragg curve. Utilizing the dedicated secondary beam course for R&D studies at HIMAC, we measured the total energy loss of $^{11}$ C ions in a scintillator block that simulates the soft tissue in human bodies. In addition to the total absorption $^{11}$ C peak, non-negligible bump-shaped contribution is observed in the energy spectrum. The origin of the bump contribution can be nuclear interaction of the incident $^{11}$ C ions with hydrogen and carbon atoms. Further studies to reduce the ambiguity in dose distribution are mentioned.

• Mycobiology
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• v.39 no.2
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• pp.79-84
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• 2011

Nuclear distribution within the extra-radical fungal structures and during spore production in the arbuscular mycorrhizae fungus Glomus intraradices was examined using an in vitro monoxenic culture system. A di-compartmental monoxenic culture system was modified using a nitrocellulose membrane and a coverglass slip for detailed observations. Nuclear distribution was observed using the fluorescent DNA binding probes SYBR Green I and DAPI. Both septate and non-septate mycelial regions were observed, but cytoplasmic contents were only found within non-septate mycelia. Nuclear fluorescent staining revealed that the non-septate hyphal region contained nuclei only with cytoplasm, and that nuclear distribution was limited by septa. Swollen hyphal bodies were often associated with septate and empty-looking hyphae. Cytoplasmic contents filled the swollen hyphal body from the non-septate hyphal region following removal of the septa. As a consequence, the swollen body developed into a new spore. These observations provide understanding about the distribution of AM fungal nuclei within extra-radical mycelia and during spore formation. The results suggest a mechanism by which the development of a cytoplasm-containing mycelium is controlled by the formation or removal of septa to efficiently maintain and proliferate essential contents. This mechanism may provide a survival strategy to the fungus.

• Journal of Embryo Transfer
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• v.14 no.3
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• pp.195-201
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• 1999

This study was carried out to improve a technique of cloned animal prodcution by preactivation of nuclear recipient oocytes with ionomycin and 6-dimethylaminopurine (6-DMAP) in rabbits. The oocytes were collected from the oviduct of superovulated rabbit at 19∼20 hours post hCG injection. The collected oocytes were preactivated and self-enucleated by treating 5 uM ionoycin for 5 min, and 2.0 mM 6-DMAP for two hours. Microsurgical removel of the chromation complex in the second polar bodies was effectively performd and single blastomere separated from 32-cell stage rabbit embryos was injected into the perivitelline space of the enculeated recipient oocyts. Follwoing electrofusion and in vitro culture for 18 hours, the nuclear transplant(NT) embryos were transferred into the uterine horns of naturally mated or synchronized recipient does. When 32 NT embryous reconstituted with preactivated oocytes were transferred to 2 recipient does, one foster doe delivered two offspring (6.3%), while not a offspring was delivered from three foster does which received 17 NT embryos reconstituted with non-preactivated oocytes. A total of 68 NT embryos reconstituted with preactivated oocytes were transferred into the uterine horns of 7 synchronized ecipient does. Among them, two recipients were pregnant and delivered three offspring(5.9%).