• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.537-547
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• 1994

Ninety seven cases of histopathologically diagnosed spontaneous canine parvovirus enteritis(CPE) were studied gross pathologically, histopathologically, immunohistochemically, to investigate histopathological types of small intestinal lesions, and antigen distributions in each pattern related to the infected age. And also, reliability of histopathological method in diagnosis of CPE was inspected with immunohistochemistry. The results were as follows : 1. Age-related occurring ratio in histopathologically diagnosed CPE was 53.6% in 4-8 weeks, 26.8% in 9-15 weeks, 8.25 in 16-19 weeks and 11.3% in 20-45 weeks of the clog age. 2. In histopathologic classification based on patterns of villi/crypts lesions of small intestine(jejunum), the ratio of A type (initial phase of necrosis of crypt epithelia, desquamated epithelial cells in the dilated lumen of the crypt) was 20.6%; the ratio of B type(middle phase of atrophy and fission of the villi, collapse of the mucosa, loss of normal crypt structure) was 62.9%, and C type(regenerative phase of the crypt architecture) was 16.5%. 3. The ratio of A, B, C type in 4-8 weeks old, respectively, was 23.5%, 61.5%, 15.4%; in 9-15 weeks old was 19.2%, 65.4%, 15.3% in 16-19 weeks old was 25.0%, 75.0%, 0.0%; and in 20-45 weeks old was 9.0%, 54.5%, 36.4%. 4. The antigen distribution in the nuclei of the crypt epithelial cells was higher than of the cytoplasm and numerous desquamated epithelial cells in dialated crypts in A type; The antigen cytoplasm and numerous desquamated epithelial cells in dialated crypts in A type; The antigen distribution in the nuclei of the collapsed crypt epithelial cells was not higher than that of the cytoplasm, crypts were lined by and filled with released viral antigens from the destructed epithelial cells in B type; and its distribution was also higher than in the epithelial cells adjacent to the tips of the villi, but it was not reacted in the regenerative crypt epithelial cells in C type. 5. Immunohistochemically detected antigen ratio in the small intestine of histopathologically diagnosed CPE was 94.6%, and this result indicates that histopathological diagnosis is very reliable method in diagnosis of CPE.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.23 no.1
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• pp.34-42
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• 2011

It's been reported that the global warming effect has invoked the ever increasing typhoon intensity and long-term sea level rise which jointly cause severe wave overtopping over breakwaters or shore dykes. A simple measure to cope with this undesirable change may be just to increase the crest height of the dykes and breakwaters. This is surely effective to prevent wave overtopping, but it also decreases the seaward visibility of coastal waterfront. In this paper, a dynamic deformable rubber membrane parapet which not only reduces wave overtopping in storm period but also secures seascapes in normal days is presented. Several optimal configurations of the parapet are proposed. Through numerical analyses using a nonlinear finite element model and hydraulic experiments, the air controlled expansion and contraction of the parapets, their behavior against wave overtopping and structural stability are investigated.

• The KIPS Transactions:PartC
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• v.15C no.5
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• pp.351-358
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• 2008

Recently, as network flooding attacks such as DoS/DDoS and Internet Worm have posed devastating threats to network services, rapid detection and proper response mechanisms are the major concern for secure and reliable network services. However, most of the current Intrusion Detection Systems(IDSs) focus on detail analysis of packet data, which results in late detection and a high system burden to cope with high-speed network environment. In this paper we propose a lightweight and fast detection mechanism for traffic flooding attacks. Firstly, we use SNMP MIB statistical data gathered from SNMP agents, instead of raw packet data from network links. Secondly, we use a machine learning approach based on a Support Vector Machine(SVM) for attack classification. Using MIB and SVM, we achieved fast detection with high accuracy, the minimization of the system burden, and extendibility for system deployment. The proposed mechanism is constructed in a hierarchical structure, which first distinguishes attack traffic from normal traffic and then determines the type of attacks in detail. Using MIB data sets collected from real experiments involving a DDoS attack, we validate the possibility of our approaches. It is shown that network attacks are detected with high efficiency, and classified with low false alarms.

• Fashion & Textile Research Journal
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• v.8 no.4
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• pp.471-475
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• 2006

The summary of a study of efficacy of Rose essential oil and Lavender essential oil against skin-inflammation caused to surfactant is as follows. As a result of protein analysis, although Rose essential oil and Lavender essential oil stimulates skin, there is no much alteration of protein. The alteration of protein's bend to be under part of 43Kd is appeared in the group treated Rose essential oil and Lavender essential oil. The stratum of the group treated by surfactant is more, the stratum of the group treated by surfactant is more thick. As the observed result of alteration of basal layer, it is seen that the group treated Rose essential oil and Lavender essential oil takes the shape of cell layer constant, and the group treated Rose essential oil is made the basal cell layer of normal structure than that of Lavender essential oil. As the observed result of alteration of Mast cell group treated by Rose essential oil and Lavender essential oil for 3 weeks Luna's stain(${\times}20$), (${\times}100$), it is observed that the damaged group treated by Lavender essential oil is numerous in alteration of mast cell's number, and Mast cell's size is larger than the damaged group.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.95-95
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• 1997

Among the various receptor molecules discovered so far the ${\beta}$2-adrenergic receptors have been regarded as excellent model systems for the so called 7 transmembrane helix receptor and have been the focus of extensive studies. For the analysis of receptor structure and function a monoclonal antibody plays a crucial role, thus providing useful tools for the study of receptor. However, because of the minute quantity of receptor molecules which could be obtained from natural sources, the generation of specific monoclonal antibody against receptor molecules from the purified receptors has been regarded as virtually impractical in consideration of cost and experimental times. The purpose of the present study was to generate and characterize a monoclonal antibody against human ${\beta}$2-adrenergic receptor. For the production of antibody, C-terminal regions of the human ${\beta}$2-adrenergic receptor was produced as a fusion protein with Glutathion S-transferase (GST) in E. Coli. The expression of the fusion protein was identified by SDS-PAGE and Western blot using monoclonal anti-GST antibody. The fusion protein was purified to an apparent homogeniety by affinity chromatography with Glutathion Sepharose CL-4B and used as an antigen for the immunization of BALB/C mice. The Production of monoclonal antibody was achieved by fusion of the immunized spleen cells and SP/2-0 myeloma cells. Positive hybridomas were screened by ELISA and were cloned by two consecutive rounds of limiting dilution. The monoclonal antibody produced in this study (mAb${\beta}$C02) was IgM type and purified by immunoaffinity chromatography using anti-mouse IgM agarose as an affinity matrix. MAb${\beta}$C02 showed strong and specific immunoreactivity against both the fusion protein and human ${\beta}$2-adrenergic receptor in ELISA and Western blot. The molecular weight of immunoreactive band was 64 kDa and exactly coincided with the previously reported molecular weight of ${\beta}$2-adrenergic recepters. The results of the present study suggest that mAb${\beta}$C02 may be used for the study of receptor function and regulation in normal or nonphysiological status.

• Journal of Christian Education in Korea
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• v.63
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• pp.317-345
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• 2020

The dissolution and reconstruction of the male-centered social structure is being requested, but the Korean church still call on women and understand women's roles by limiting them based on traditional 'normal family ideology' and matherhood discourse. However, considering women's various aspects of life, life cycle, and individuality, confining women to existing biological maternal discourse is not suitable to help women grow as subjective leaders and contribute to society. The Korean church needs to find a new curriculum that encourages women to form subjective beliefs. In the life of Christian women of the period of port-opening, we can examine the process of the Korean Christian women establishing the subjectivity of the challenges of Protestant theology, which included stereotypes, gender division of labor, and matherhood discourse. Korean Christian women shared the oppressive experiences of traditional patriarchy after passing silent and receptive perceptions, forming a subjective perception of their injustice and seeking liberation. And it was able to act as a subject of faith by forming a procedural and constructive awareness within a sympathetic and relational community. The Korean church should reconstruct the Christian women's curriculum by reflecting on the curriculum that women formed themselves over 100 years ago.

• Korean Journal of Animal Reproduction
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• v.24 no.2
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• pp.217-222
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• 2000

This study was conducted to investigate the effect of quiescent treatment of the donor cells on the nuclear remodeling and in vitro development of fetal fibroblast cell-cloned bovine embryos. Serum starved, confluent and nonquiescent cycling fetal fibroblast cells were transferred into the enucleated oocytes. About 20∼25% of nuclear transfer embryos fused with a serum starved or confluent cell extruded a polar body, which was slightly lower than that of nontreated control (36%). About 49∼51% of nuclear transfer embryos fused with a serum starved or confluent cell had a single chromatin clump, which was slightly higher than that of nontreated control (40%). The proportion of embryos with a single chromatin clump was significantly higher (P＜0.01) in nuclear transfer embryos without showing a polar body (60.5%) than with a polar body (4.7%). Development rates to the blastocyst stage were 21.7% and 20.9% when serum starved and confluent cells were transferred, which were slightly higher than that of control (14.1 %). The result of this study suggests that quiescent treatment by serum starvation or growth to confluency of donor cells could increase the number of embryos with a normal chromatin structure, which results in increased in vitro development.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.8
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• pp.14-18
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• 2018

Generally. the Ohmic's law is an important factor to increase the conductivity in a micro device. So it is also known that the Ohmic contact in a semiconductor device is import. The PN junction as a structure of semiconductor involves the depletion layer, and this depletion layer induces the non linear electrical properties and also makes the Schottky contact as an intrinsic characteristics of semiconductor. To research the conduction effect of insulators in the semiconductor device, $SiO_2$ thin film and $V_2O_5/SiO_2$ thin film were researched by using the current-voltage system. In the nano electro-magnetic system, the $SiO_2$ thin film as a insulator had the non linear Schottky contact, and the as deposited $V_2O_5$ thin film had the linear Ohmic contact owing to the $SiO_2$ thin film with superior insulator's properties, which decreases the leakage current. In the positive voltage, the capacitance of $SiO_2$ thin film was very low, but that of $V_2O_5$ thin film increased with increasing the voltage. In the normal electric field system, it was confirmed that the conductivity of $V_2O_5$ thin film was increased by the effect of $SiO_2$ thin film. It was confirmed that the Schottky contact of semiconductors enhanced the performance of electrical properties to increased the conductivity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.2
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• pp.535-541
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• 2020

FCAW is used mainly in the welding of carbon steel and alloy steel because it can be welded in all positions and can obtain excellent quality at sites with variable working conditions. Recently, many studies in Korea have estimated the fatigue strength, residual stress, and deformation, and to develop a fillet welding process. On the other hand, there have been few studies of the mechanical properties based on the strength, macro and magnetic particle test results for fillet welding. This study shows the following results through fillet welding, macro testing and strength testing using SM490A (solid-structure rolled steel) for thick plates using SS400 (rolled steel) for the upper plate and FCAW. The hardness test, macro test and magnetic particle test were then conducted. The hardness tests showed that all result values were smaller than the KS B 0893 standard values of 350Hv. The macro-test showed that each type of welded part was in a normal organic state and that there were no internal errors, bubbles, or impurities on the front of the welded part. Therefore, there were no concerns about lamination. The magnetic particle examination showed no problems.

• Journal of Applied Biological Chemistry
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• v.52 no.2
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• pp.65-69
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• 2009

In this study, we tried to find the subject to inhibit H. pylori from Cheongmoknosang mulberry leaves extracts and to purify and identify them. Total phenolic compounds of hot water and 80% ethanol extracts are 17.6 and 16.1 mg/g. The activity of H. pylori inhibition at 80% ethanol extracts was determined as 15mm clear zone. The purification of inhibitory compounds were carried on $C_{18}$ column and MCI-gel CHP-20 column chromatography which were used a gradient procedure as increasing ethanol in $H_2O$. The chemical structure of purified inhibitory compounds on H. pylori were identified chlorogenic acid, caffeic acid, and rosmarinic acid by FAB-MS, $^1H-NMR$, $^{13}C-NMR$ and IR spectrum.