Journal of Applied Biological Chemistry

  • 제52권2호
  • /
  • Pages.65-69
  • /
  • 2009
  • /
  • 1976-0442(pISSN)
  • /
  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지
DOI QR코드

DOI QR Code

청목노상 뽕잎으로부터 Helicobacter pylori 억제물질의 정제 및 동정

Purification and Identification of Inhibitory Compounds from Cheongmoknosang Mulberry Leaves (Morus alba. L.) on Helicobacter pylori

  • Cho, Young-Je (Department of Food Engineering, Kyungpook National University) ;
  • Lee, Kyung-Hwan (Department of Food Engineering, Kyungpook National University) ;
  • Cha, Woen-Seup (Department of Food Engineering, Kyungpook National University) ;
  • Ju, In-Sik (Department of Food Engineering, Kyungpook National University) ;
  • Yun, Dong-Hyuck (Department of Food Engineering, Kyungpook National University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Lee, Seon-Ho (The Institute of Marine Biotechnology, Andong National University) ;
  • Kim, Myung-Uk (Gyungbuk Institute for Marine Bio-Industry) ;
  • Kim, Jeung-Hoan (NIP Biotech.) ;
  • Chun, Sung-Sook (NIP Biotech.)
  • 발행 : 2009.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 청목노상 추출물로부터 Helicobacter pylori를 억제하는 물질을 정제 및 동정을 실시하였다. 80% 에탄올 추출물의 페놀성 물질의 함량은 16.1 mg/g으로 측정되었으며, H. pylori 저해활성은 clear zone이 15 mm로 나타났다. 저해물질의 분리는 $C_{18}$ column을 이용하여 분리한 결과 9개의 fraction으로 분리되었으며, H. pylori 저해활성을 나타낸 fraction 7과 8을 MCI-gel CHP-20 column을 이용하여 normal phase type인 ethanol 100%에서 0%로 농도를 감소시키며 10 mL/min의 유속으로 용출한 결과 6개의 단일 물질 분획을 얻을 수 있었다. 그 중 세 가지의 물질이 H. pylori저해활성을 보여 FAB-Mass, $^1H$와 $^{13}C$ NMR과 IR spectrum을 사용하여 구조 동정한 결과 chlorogenic acid, caffeic acid 및 rosmarinic acid인 것으로 확인되었다.

In this study, we tried to find the subject to inhibit H. pylori from Cheongmoknosang mulberry leaves extracts and to purify and identify them. Total phenolic compounds of hot water and 80% ethanol extracts are 17.6 and 16.1 mg/g. The activity of H. pylori inhibition at 80% ethanol extracts was determined as 15mm clear zone. The purification of inhibitory compounds were carried on $C_{18}$ column and MCI-gel CHP-20 column chromatography which were used a gradient procedure as increasing ethanol in $H_2O$. The chemical structure of purified inhibitory compounds on H. pylori were identified chlorogenic acid, caffeic acid, and rosmarinic acid by FAB-MS, $^1H-NMR$, $^{13}C-NMR$ and IR spectrum.

키워드

참고문헌

  1. Barnes HM, Reldman JR and White WV. (1950) Isochlorogenic acid. Isolation from Coffee and Structure Studies. J. Am. Chem. Soc., 72, 4178-4183 https://doi.org/10.1021/ja01165a095
  2. Cha JY, Lee JY, Hoang IS, Whangbo D, Choi PW, Lee WC, Kim JW, Kim SY and Rhee SJ (2003) Analysis of functional components of leaves of different mulberry cultivars. J. Korean Soc. Food Sci. Nutr. 32, 15-21 https://doi.org/10.3746/jkfn.2003.32.1.015
  3. Cho YJ, Kwon OJ, Chun SS, An BJ and Kim JH (2008) Biological and antimicrobial activity of Portulaca oleacea. J. Korean Soc. Appl. Biol. Chem. 51, 49-54
  4. Chun SS, Vattem DA, Lin YT and Shetty K (2005) Phenolic antioxidants from clonal oregano(Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry. 40, 809-816 https://doi.org/10.1016/j.procbio.2004.02.018
  5. Folin O and Denis W (1912) On phosphotungastic-phosphomolybetic compounds as color reagents. J. Biol. Chem., 12, 239-249
  6. Goodwin CS and Worsiey BW (1993) Helicobacter pylori: Biological and clinical ratice. CPC press, Inc. USA
  7. Hong YL, Kim NH, Ahn C, Lee HY and Kim JD (2000) Studies on the biological activities of the extracts from Hovenia dulcis THUNB, Inst. Agr. Sci., Kangwon Nat'l. Univ. 11, 1-11
  8. Hunt RH (1996) Eradication of Helicobacter pylori infection. Am. J. Med. 100, 42S-50S
  9. Kang JH and Lee MS (2005) In vitro inhibition of Helicobacter pylori by Enterococcus faecium GM-1. Can. J. Microbial. 51, 629-636 https://doi.org/10.1139/w05-044
  10. Kelly CJ, Hanllff CR and Carmack M (1976) The Polyphenolic acids of Lithospermum ruderale. 11. Carbon-13 Nuclear Magnetic Resonance of Lithospermic and Rosemarinic acids. J. Org. Chem., 41, 449-455 https://doi.org/10.1021/jo00865a007
  11. Kil JH, Jung KO, Lee HS, Hwang IK, Kim YJ and Park KY (2004) Effects of kimchi on stomach and colon health of Helicobacter pylori-infected volunteers. J. Food. Sci. Nutr. 9, 161-166 https://doi.org/10.3746/jfn.2004.9.2.161
  12. Kim NY (2006) The effect of antibiotic resistance on the eradication of Helicobacter pylori. Kor. .J Gastroenterol. 47, 82-86
  13. Kim JS, Kang SS, Lee MW and Kim OK (1995) Isolation of flavonoids from the leaves of Aralia continentails. Korean J. Pharmacogn. 26, 239-243
  14. Kwon YS, Won HM and Kim CM (2000) Flavonoids from Indigofera psedo-tinctoria stem. Kor. J. Pharmacogn. 31, 280-283
  15. Lee JH and Lee SR (1994) Some physiological activity of phenolic substance in plant foods. Korean J. Food Sci, Technol. 26, 317-323 https://doi.org/10.1021/jf60163a004
  16. Lee KJ and Row KH (2004) Comparison of extraction methods form aglycone isoflavones from Korean Soybean, Korean J. Biotechnol. Bioeng. 19, 421-426
  17. Lee MJ, Chang YR and Yim NH (1998) Chemical structure of Polyphenols isolated from cacao bean and their inhibitory effect on ACE. Agricultural Chemistry and Biotechnology, 41, 110-117
  18. Lee MK, Kim YG, An SW, Kim MH, Lee JH and Lee HY (1999) Biological activities of Hovenia dulcis THUNB, Korean J. Medicinal Crop Sci. 7, 185-192
  19. Lee SH, Lim CY, Lee KH, Yeo SJ, Kim BJ, Kim SJ, Cho MJ, Rhee KH and Kook YH (1999) rpoB Gene analysis of Helicobacter pylori. J. Korean Soc. Microbial. 34, 401-408
  20. McClure WJ, Harborne JB, Mabry TJ and Mabry H (1975) The flavonoids (part 2), In : Physiology and Functions of Flavonoids. Academic Press Inc., New york, USA. 1033-1035
  21. NSOK (2003) Annual report on the cause of death statistics. National Statistical Office of Korea, Seoul, Korea
  22. Park JG, Yun SY, Oh S, Shin JG and Baek YJ (2003) Probiotic characteristics of Lactobacillus acidophilus KY1909 isolated from Korean brest-fed infant. Kor. J. Food Sci. Technol. 35, 1244-1247
  23. Shetty K (1997) Biotechnology to harness the benefits of dietary phenolics: Focus on Laminaceae, Asia Pacific J. Clin. Nutr. 6, 162-171
  24. Shin KH, Young HS, Lee TW and Choi JS (1995) Studies on the chemical component and antioxidant effects of Solanum lyratum. Korean J. Pharmacogn. 26, 130-138
  25. Suerbaum S, Michetri P (2002) Helicobacter pylori infection. N Engl. J. MED. 347, 1175-1186 https://doi.org/10.1056/NEJMra020542
  26. Won BR, Song EH, Kang GH, Chang MW and Yoon YH (2001) Inhibition effect of Lactobacillus heiviticus CU631 on urease and vacuolating toxin activity of Helicobacter pylori. J. Anim. Sci. Technol. 43, 931-940

피인용 문헌

  1. Purification and Identification of Inhibitory Compounds on Helicobacter pylori from Cheongmoknosang Callus for Biomass vol.20, pp.3, 2010, https://doi.org/10.5352/JLS.2010.20.3.374
  2. Evaluation of In Vivo Safety of Inhibitory Compounds from Cheongmoknosang Mulberry Leaves against Helicobacter pylori vol.40, pp.10, 2011, https://doi.org/10.3746/jkfn.2011.40.10.1404
  3. Evaluation of Medicinal Activity on Isolated Inhibitory Compounds against Helicobacter pylori from Cheongmoknosang Mulberry Leaves vol.54, pp.4, 2011, https://doi.org/10.3839/jabc.2011.043
  4. Rosmarinic acid: new aspects vol.12, pp.1, 2013, https://doi.org/10.1007/s11101-013-9282-8
  5. Production of Inhibitory Compounds against Helicobacter pylori by Culture Condition of Morus alba cv. Cheongmoknosang Callus vol.23, pp.3, 2013, https://doi.org/10.5352/JLS.2013.23.3.368
  6. Inhibitory Activity against Helicobacter pylori of Isolated Compounds from Pinus koraiensis Siebold et Zucc Leaves vol.59, pp.1, 2016, https://doi.org/10.3839/jabc.2016.005