• Genomics & Informatics
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• v.20 no.4
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• pp.46.1-46.7
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• 2022

Influenza A virus (IAV) is the most widespread pathogen causing human respiratory infections. Although polymerase chain reaction (PCR)-based methods are currently the most commonly used tools for IAV detection, PCR is not ideal for point-of-care testing. In this study, we aimed to develop a more rapid and sensitive method than PCR-based tools to detect IAV using loop-mediated isothermal amplification (LAMP) technology. We designed reverse-transcriptional (RT)-LAMP primers targeting the hemagglutinin gene. RNAs from reference H1N1 and H3N2 showed specific RT-LAMP signals with the designed primers. We optimized the reaction conditions and developed universal reaction conditions for both LAMP assays. Under these conditions, the detection limit was 50 copies for both RT-LAMP assays. There was no non-specific signal to 19 non-IAV respiratory viruses, such as influenza B virus, coronaviruses, and respiratory syncytial viruses. Regarding the reaction time, a positive signal was detected within 25 min after starting the reaction. In conclusion, our RT-LAMP assay has high sensitivity and specificity for the detection of the H1 and H3 subtypes, making it suitable for point-of-care IAV testing.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.20 no.4
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• pp.1233-1240
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• 1996

Conventional measurement methods using ultrasonic wave or x-ray, eddy current for non-destructive testing(NDT) in nuclear power plants and other industrial plants have been utilized as the method of contact with objects to be inspected. For this reason these methods require relatively much time and inspection area is limited by the location of probe or film. But holograpic interferometry which is a non-contact optical measurement method using a coherent light source has an advantage that quantative measurement can be performed at a time. In this paper a new method using realtime holographic interfreometry and image processing for detecting internal flaws of pressure vessels is presented.

• The KIPS Transactions:PartA
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• v.8A no.2
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• pp.125-132
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• 2001

IBM developed the MPI-IO, we call it MPI-2, on the General Parallel File System. We designed and implemented various Matrix Multiplication Benchmarks to evaluate its performances. The MPI-IO on the General Parallel File System shows four kinds of data access methods : the non-collective and blocking, the collective and blocking, the non-collective and non-blocking, and the split collective operation. In this paper, we propose benchmarks to measure the IO time and the computation time for the data access methods. We describe not only its implementation but also the performance evaluation results.

• Earthquakes and Structures
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• v.8 no.6
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• pp.1325-1348
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• 2015

Real-time hybrid testing (RTHT) involves virtual splitting of the structure into two parts: physical substructure that contains the key region of interest which is tested in a laboratory and numerical substructure that contains the remaining part of the structure in the form of a numerical model. This paper numerically assesses four step-by-step integration methods (Central difference method (CDM), Operator splitting method (OSM), Rosenbrock based method (RBM) and CR-integration method (CR)) which are widely used in RTHT. The methods have been assessed in terms of stability and accuracy for various realistic damping ratios of the physical substructure. The stability is assessed in terms of the spectral radii of the amplification matrix while the accuracy in terms of numerical damping and period distortion. In order to evaluate the performance of the methods, five carefully chosen examples have been studied - undamped SDOF, damped SDOF, instantaneous softening, instantaneous hardening and hysteretic system. The performance of the methods is measured in terms of a non-dimensional error index for displacement and velocity. Based on the error indices, it is observed that OSM and RBM are robust and performs fairly well in all the cases. CDM performed well for undamped SDOF system. CR method can be used for the system showing softening behaviour. The error indices indicate that accuracy of OSM is more than other method in case of hysteretic system. The accuracy of the results obtained through time integration methods for different damping ratios of the physical substructure is addressed in the present study. In the presence of a number of integration methods, it is preferable to have criteria for the selection of the time integration scheme. As such criteria are not available presently, this paper attempts to fill this gap by numerically assessing the four commonly used step-by-step methods.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.11
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• pp.6432-6439
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• 2014

The NAUT (Non-contact Air coupled Ultrasonic Testing) technique is one of the ultrasonic testing methods that enables non-contact ultrasonic testing by compensating for the energy loss caused by the difference in acoustic impedance of air with an ultrasonic pulser receiver, PRE-AMP and high-sensitivity transducer. As the NAUT is performed in a state of steady ultrasonic transmission and reception, testing can be performed on materials of high or low temperatures or specimens with a rough surface or narrow part, which could not have been tested using the conventional contact-type testing technique. For this study, the internal defects of spot weld, which are often applied to auto parts, and CFRP parts, were tested to determine if it is practical to make the NAUT technique commercial. As the spot welded part had a high ultrasonic transmissivity, the result was shown as red. On the other hand, the part with an internal defect had a layer of air and low transmissivity, which was shown as blue. In addition, depending on the PRF (Pulse Repetition Frequency), an important factor that determines the measurement speed, the color sharpness showed differences. With the images obtained from CFRP specimens or an imaging device, it was possible to identify the shape, size and position of the internal defect within a short period of time. In this paper, it was confirmed in the above-described experiment that both internal defect detection and image processing of the defect could be possible using the NAUT technique. Moreover, it was possible to apply NAUT to the detection of internal defects in the spot welded parts or in CFRP parts, and commercialize its practical application to various fields.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.1
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• pp.19-26
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• 2016

In the consumer products industry, it has been highly desirable to develop objective test methods to replace subjective evaluation methods. In developing an objective test method, subjective evaluation data should be on a linear scale. According to Thurstone's theory of comparative judgment, a%-preference from a paired-comparison test can be converted to a linear-scale value. The required number (N) of paired-comparison tests increases dramatically as the number of products increases. This problem should be solved by classifying the total products into several subgroups consisting of 3-4 products in each group. By doing so, it can not only significantly reduce the number of required paired-comparison tests, but it can also obtain more reliable, reproducible data.

• Composites Research
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• v.32 no.6
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• pp.395-402
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• 2019

The RFI process is an OoA process that fiber mats and resin films are laminated and cured in a vacuum bag. In case that resin film is insufficient to fill empty space in fibers, it makes void defect in composites and this void decrease mechanical properties of the composites. For this reason, non-destructive testing is usually used to evaluate void of manufactured composites. So, in this study, a manufacturing method of standard void specimens, which are able to be used as references in non-destructive testing, was proposed by controlling resin film thickness in the RFI process. Also, a fiber compaction test was proposed as a method to set the resin film thicknesses depending on target voids of manufacturing panels. The target void panels of 0%, 2%, and 4% were made by the proposed methods, and signal attenuation depending on void was measured by non-destructive testing and image analysis. In addition, voids of specimens for tensile, in-plane, short beam and compressive tests were estimated by signal attenuation, and mechanical properties were evaluated depending on the voids.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2005.11a
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• pp.205-208
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• 2005

We have fuzzy hypotheses testing from Bayesian statistics with ideas from fuzzy sets theory to generalize Bayesian methods both for samples of fuzzy data and for prior distributions with non-precise parameters. Appling the principle of agreement index, the posterior odds ratio in the favor of hypotheses $H_0$ is equal to product of the fuzzy odds ratio and the fuzzy likelihood ratio. If the Posterior odds ratio exceeds the grade judgement, we accept the hypothesis $H_0$ for the degree.

• Journal of Applied Reliability
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• v.2 no.1
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• pp.23-32
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• 2002

As the function of digital products gets diverse and complex, more than 30~40% job of software developing and testing group within its development cycle, concentrates on the software testing[1]. To cope with consumers'quickly changing needs, development cycle time gets short, and the number of model and test items increase steadily This is why automating software basic function and UI(User Interface) verification is needed [4][5][6]. This paper draws many strength and weakness defines Input/Output through integrating mobile phone, DVDP, PDA, and Black-Box (Intrusive (mobile) or Non-Intrusive(DVDP, PDA)) testing systematically, which brought above the 64% average of automation rate, and ensure 80% of test coverage [4][5][6].

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1993.10a
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• pp.273-278
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• 1993

Conventional measurement methods for non-destructive testing(NDT) in nuclear power plants other industrial plants have been performed as the methods of contact with objects to be inspect, but those methods have been taken relatively much time to be inspected. Holographic interferometry which is a non-contact optical measurement method using a coherent light can overcome these demerit, and also has an advantage that the quantitative measurement of small deformation for large areas can be accomplished at a time with high precision. In this paper the comparisons of the experimental results from holographic interferometry with those form the finite element method(FEM) and the analytical solutions of elastic equation are discussed.