• Clinical and Experimental Pediatrics
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• v.46 no.12
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• pp.1186-1193
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• 2003

Purpose : Fragile sites are points on chromosomes which tend to break non-randomly when exposed to specific chemical agents or conditions of tissue culture. The chromosomal break induced by the antineoplastic drug, 1-${\beta}$-D-arabinofuranosyl-cytosine(Ara-c), was investigated to study the laboratory conditions in which the incidence of chromosomal break could be enhanced. Besides, the fragile sites induced by Ara-C were investigated and compared to the already known locations of the specific chromosomal alterations observed in specific neoplasms. Methods : T-lymphocytes from theree normal males and three females were cultured for 48 hours. Cells from each individual were exposed to the Ara-C for an additional 24 hours. After the caffeine was added during the last six hours culture, the metaphase chromosomes were prepared following the conventional method. A site was considered fragile if it was found to break two or more per 100 chromosomal breaks in more than four of six individuals tested. Results : Ara-C induced 252.1 chromosomal breaks per 100 mitotic cells and this result was significantly higher than that of the control, which induced 25.2 breaks(P<0.05). The incidence of the chromosomal break by Ara-C was higher, if cultured in the MEM-FA, which has no folic acid, than in the RPMI 1640 which contains enough folic acid(P<0.05). The most common break site by Ara-C was 3p14.2(FRA3B). There were 20 fragile sites induced by Ara-C. Among these 20 fragile sites, seven coincided with the locations of the mapped oncogenes, JUN, SKI, REL, N-MYC, FHIT, MET, ETS-1, and FOS. Conclusion : S phase specific chemotherapeutic agent, Ara-C, induced the expression of the chromosomal fragile sites effectively using the T-lymphocyte in vitro. Some of the fragile sites by Ara-C highly coincided with the oncogenes and neoplasm specific chromosome breakpoints. In this regard, the fragile sites reported here could provide the unknown neoplasm related chromosomal alternation points.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.7
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• pp.4516-4521
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• 2014

Digital watermarking for digital image authentication and integrity schemes are based on fragile watermarking and can detect any modifications in a watermark embedded image by comparing the embedded watermark with the regenerated watermark. Therefore, the digital watermark for image authentication and integrity should be erased easily when the image is changed by digital image processing, such as scaling or filtering etc. This paper proposes an effective tamper detection scheme for digital images. In the proposed scheme, the original image was divided into many non-overlapping $2{\times}2$ blocks. The digital watermark was divided into two LSB of each block and the image distortion was imperceptible to the human eye. The watermark extraction process can be used to determine if the watermarked image has been tampered. The experimental results successfully revealed the effectiveness of the proposed scheme.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.4
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• pp.1499-1513
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• 2014

This paper proposes a novel blind image watermarking scheme exploiting Block Truncation Coding (BTC). Most of existing BTC-based watermarking or data hiding methods embed information in BTC compressed images by modifying the BTC encoding stage or BTC-compressed data, resulting in watermarked images with bad quality. Other than existing BTC-based watermarking schemes, our scheme does not really perform the BTC compression on images during the embedding process but uses the parity of BTC quantization data to guide the watermark embedding and extraction processes. In our scheme, we use a binary image as the original watermark. During the embedding process, the original cover image is first partitioned into non-overlapping $4{\times}4$ blocks. Then, BTC is performed on each block to obtain its BTC quantized high mean and low mean. According to the parity of high mean and the parity of low mean, two watermark bits are embedded in each block by modifying the pixel values in the block to make sure that the parity of high mean and the parity of low mean in the modified block are equal to the two watermark bits. During the extraction process, BTC is first performed on each block to obtain its high mean and low mean. By checking the parity of high mean and the parity of low mean, we can extract the two watermark bits in each block. The experimental results show that the proposed watermarking method is fragile to most image processing operations and various kinds of attacks while preserving the invisibility very well, thus the proposed scheme can be used for image authentication.

• The KIPS Transactions:PartB
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• v.12B no.4 s.100
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• pp.421-428
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• 2005

A new method of Semi-fragile image watermarking which ensures the integrity of the contents of digital image is presented. Proposed watermarking scheme embeds watermark in the form of quantization noise on the wavelet transform coefficients in a specific mid frequency subbands selected from a wavelet packet decomposition based on energy distribution of wavelet transform coefficients. By controlling the strength of embedded watermark using HVS (Human Visual System) characteristic, it is imperceptible by a human viewer while robust against non-malicious attack such as compression for storage and/or transmission. When an attack is applied on the original image, it is highly probable that wavelet transform coefficients not only at the exact attack positions but also the neighboring ones are modified. Therefore, proposed authentication method utilizes whether both current coefficient and its neighbors are damaged. together. So it can efficiently detect and accurately localize attacks inflicted on the content of original image. Decision threshold for authentication can be user controlled for different application areas as needed.

• The Korea Journal of Herbology
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• v.38 no.4
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• pp.45-52
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• 2023

Objectives : Fine dust caused by environmental pollution cause oxidative damage and skin aging. In this study, The possibility of using the Codium fragile extract (CFE) as an anti-aging product for skin improvement was evaluated by confirming the protective effect of skin cells from PM10 (particulate matter 10) through inhibition of ROS and MMPs. Methods : In this study, elastase and collagenase inhibitory activities were evaluated. Cell viability was evaluated by treating keratinocytes (HaCaT cell line) with CFE at various concentrations. The cytoprotective effect from PM10 in keratinocyteswas evaluated using the 3-[4,5-dimethylthiazol]-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay. ROS (reactive oxygen species) was measured in keratinocytes damaged by PM10 using DCF-DA (2′,7′-dichlorofluorescin diacetate) fluorescence staining. As an anti-aging effect of CFE, MMP-1 (matrix metalloproteinase-1) and MMP-1 (matrix metalloproteinase-9) inhibitory activities were evaluated. Results : As a result, CFE decreased the activity of elastase and collagenase. As a result of evaluating the toxicity of CFE, it is non-toxic at a concentration of 10 to 80 ㎍/㎖. Although cell viability of HaCaT cells treated with PM10 decreased, cell viability increased by 38% when treated with CFE 80 ㎍/㎖. Also, ROS decreased by 8.4%, and MMP-1 and MMP-9 decreased at CFE 80 ㎍/㎖. Conclusions : CFE showed excellent cell protection effect, and it is considered that it can be used in anti-aging products for skin improvement by effectively inhibiting ROS and MMPs from keratinocyte damage caused by fine dust.

• Proceedings of the KIEE Conference
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• 2008.04a
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• pp.209-210
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• 2008

본 논문에서는 제어기에 승산형 섭동을 가지는 제어기와 이산시간 특이시스템을 안정화시키는 비약성 제어기 설계방법을 제시한다. 강인 비약성 제어기가 존재할 조건과 제어기 설계방법을 블록최적화가 가능한 선형 행렬부등식 접근방법으로 제안한다. 또한, 제어기의 약성 성도를 표시하는 비약성 척도를 동시에 계산함으로 승산형 섭동의 제어기 최대 변동정도를 제시한다. 제안한 비약성 제어기는 제어기의 이득 변동에도 불구하고 이산 시간 특이시스템의 강인 안정성을 보장한다.

• The KIPS Transactions:PartD
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• v.11D no.6
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• pp.1335-1340
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• 2004

This study introduces a technology utilizing digital images as electronic money by inserting watermark into the images. Watermarking technology assign contents ID to images and inserts the contents ID into the images in an unnoticeable way. The server that manages the issue and the usage of mage electronic money (called ‘WaterCash’ hereafter) stores issued contents ID to database and manage them as electronic money. WaterCash guarantees anonymity and prevents the forgery and modification of WaterCash based on semi-fragile watermarking technique. In addition, WaterCash is transferable and the illegal use of WaterCash can be prevented based on the watermarking technology. The watermarking .technology used in this paper was designed to be robust to image compression but vulnerable to intentional or non-intentional Image processing.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.21 no.3
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• pp.401-407
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• 2012

The recent manufacturing process in the thin wafers and flat panel necessitate new approaches to reduce handling fragile and surface-sensitive damage of components. This paper presents a new pneumatic levitation for non-contact handling of parts and substrates. This levitation can achieve non-contact handling by blowing air into an air pressure pick-up head with radial passages to generate a negative pressure region. Negative pressure is caused by the radial air flow by nozzle throat and through holes connecting to the bottom region. The numerical analysis deals with the levitational motion with different design factors. The dynamic motion is examined in terms of force balance(dynamic equilibrium) occurring to the flow field between two objects. The stable equilibrium position and the safe separation distance are determined by analyzing the local pressure distribution in the fluid motion. They make considerable design factors consisting the air pressure pick-up head. As a result, in case that the safe separation distance is beyond 0.7mm, the proposed pick-up head can levitate stably at the equilibrium position. Furthermore, it can provide little effect of torque, and obtain more wide picking region according to the head size.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4281-4287
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• 2014

Background: Methylation of tumor suppressor genes has been investigated in all kinds of cancer. Tumor specific epigenetic alterations can be used as a molecular markers of malignancy, which can lead to better diagnosis, prognosis and therapy. Therefore, the aim of this study was to evaluate the association between gene hypermethylation and expression of fragile histidine triad (FHIT), glutathione S-transferase P1 (GSTP1) and p16 genes and various clinicopathologic characteristics in primary non-small cell lung carcinomas (NSCLC). Materials and Methods: The study included 28 primary non-small cell lung carcinomas, where an additional 28 tissue samples taken from apparently normal safety margin surrounding the tumors served as controls. Methylation-specific polymerase chain reaction (MSP) was performed to analyze the methylation status of FHIT, GSTP1 and p16 while their mRNA expression levels were measured using a real-time PCR assay with SYBR Green I. Results: The methylation frequencies of the genes tested in NSCLC specimens were 53.6% for FHIT, 25% for GSTP1, and 0% for p16, and the risk of FHIT hypermethylation increased among patients with NSCLC by 2.88, while the risk of GSTP1 hypermethylation increased by 2.33. Hypermethylation of FHIT gene showed a highly significant correlation with pathologic stage (p<0.01) and a significant correlation with smoking habit and FHIT mRNA expression level (p<0.05). In contrast, no correlation was observed between the methylation of GSTP1 or p16 and smoking habit or any other parameter investigated (p>0.05). Conclusions: Results of the present study suggest that methylation of FHIT is a useful biomarker of biologically aggressive disease in patients with NSCLC. FHIT methylation may play a role in lung cancer later metastatic stages while GSTP1 methylation may rather play a role in the early pathogenesis.

• Proceedings of the KIEE Conference
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• 2009.07a
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• pp.1978.1_1979.1
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• 2009

In this paper, we have proposed and demonstrated a tonometry sensor array for measuring arterial pulse pressure. A sensor module consists of 7 piezoresistive pressure sensor array. Wire-bonded connection was provided between silicon chip and lead frame. PDMS(poly-dimethylsiloxane) was coated on the sensor array to protect fragile sensor while faithfully transmitting the pressure of radial artery to the sensor. Tonometric pulse pressure can be measured by this packaged sensor array that provides the pressure value versus the output voltage.