• 대한약침학회지
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• 제27권2호
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• pp.154-161
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• 2024

Objectives: The objective of this study was to assess the genotoxicity of a no-pain pharmacopuncture (NPP) extract developed in 2022 using a bacterial reverse mutation assay, aiming to further substantiate the safety profile of NPP. Methods: The genotoxicity evaluation involved a bacterial reverse mutation assay to assess the mutagenic potential of NPP extracts with and without metabolic activation. Histidine-requiring Salmonella typhimurium strains (TA98, TA100, TA1535, and TA1537) and tryptophan-requiring Escherichia coli strains (WP2uvrA) were used in the assay. Results: The NPP extract did not induce a revertant colony count exceeding two times that of the negative control at any dose level in any of the tested strains, both with and without metabolic activation. Additionally, no growth inhibition or precipitation was observed in the presence of NPP. Conclusion: Based on the findings, it can be concluded that the NPP extract exhibited no mutagenic potential in the in vitro genotoxicity tests conducted.

• 대한약침학회지
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• 제26권4호
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• pp.366-372
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• 2023

Objectives: We aimed to evaluate the genotoxicity of a recently developed no-pain pharmacopuncture (NPP) targeting muscle relaxation and analgesia using the micronucleus test. Methods: To evaluate the potential of NPP extracts to induce micronuclei in rat bone marrow cells, a micronucleus test was performed using male Sprague-Dawley rats. The test substance NPP was administered intramuscularly at concentrations of 0.25, 0.5, and 1 mL/animal. Saline was used as the negative control and cyclophosphamide as the positive control. Results: No NPP treatment-related deaths or abnormal changes in general appearance were observed at any dose level during the experimental period. No statistically significant differences in body weight were observed in any of the NPP dose groups compared to the saline negative control group. NPP did not cause a significant increase in the incidence of micronucleated polychromatic erythrocytes (PCEs) and PCEs or in the ratio of PCE-to-total erythrocytes. Conclusion: The NPP extract did not exhibit genotoxic in Sprague-Dawley rat bone marrow cells under the conditions of this study. Further toxicity studies of the NPP extract are required.

• 대한약침학회지
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• 제27권1호
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• pp.38-46
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• 2024

Objectives: Genotoxicity is evaluated through a chromosomal aberration test using cultured mammalian cells to determine the toxicity of no-pain pharmacopuncture (NPP), which has recently been used to treat musculoskeletal pain disorders in Korean medical clinical practice. Methods: An initial test was performed to determine the dosage range of the NPP, followed by the main test. In this study, NPP doses of 10.0, 5.0, and 2.5%, and negative and positive controls were tested. An in vitro chromosome aberration test was performed using Chinese hamster lung cells under short-term treatment with or without metabolic activation and under continuous treatment without metabolic activation. Results: Compared with the saline negative control group, NPP did not significantly increase the frequency of chromosomal abnormalities in Chinese hamster lung cells, regardless of the presence or absence of metabolic activation. Additionally, the number of cells with structural chromosomal abnormalities was significantly higher in the positive control group than that in the negative control group that received saline. Conclusion: Based on the above results, the chromosomal abnormality-producing effect of NPP was determined to be negative under these test conditions.

• 대한약침학회지
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• 제26권1호
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• pp.86-93
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• 2023

Objectives: This study aimed to evaluate the potential toxicity of a recently developed and clinically used No-Pain pharmacopuncture (NPP) solution. We also assessed the lethal dose of the NPP agent following a single intramuscular injection in Sprague-Dawley (SD) rats. Methods: Animals were divided into two groups: the NPP test material group and the normal saline control group. A single intramuscular injection of the NPP agent (1.0 mL/animal) was administered to rats of the NPP test material group. The control group rats received the same volume of normal saline. Both female and male rats were included in each group. All rats were monitored for clinical signs and body weight changes for 14 days after administration of the test substance or saline. At the end of the observation period, a gross necropsy was conducted and localized tolerance at the injection site was analyzed. Results: No mortality was observed in the NPP test material and control groups. Moreover, no test substance-related effects were observed on clinical signs, body weight, necropsy findings, and localized tolerance at the injection site. Conclusion: The approximate lethal dose of the NPP agent is greater than 1.0 mL/animal under the conditions used in this study. Additional toxicity evaluations and clinical studies are needed to confirm the safety of NPP use in clinical practice.

• 대한약침학회지
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• 제13권3호
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• pp.109-120
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• 2010

Objective : This study was to investigate the clinical effect of Rubiae Radix pharmacopuncture on knee joint pain. Methods : The patients were treated by Rubiae Radix pharmacopuncture, Acupuncture, herbal medicine. To evaluate the effect of the treatment, The patient's symptoms were assessed by Visual Analogue Scale(VAS) Score, Knee injury and osteoarthritis outcome score(KOOS) and physical examinations. Results : In all cases, the pain was reduced significantly according to improvement of VAS score, KOOS and active Range Of Motion(ROM) of knee joint. VAS score and KOOS of all cases were decreased. In two cases, ROM of knee joint has improved. But in one case, ROM of knee joint has still been restricted. But there are no noticeable changes in physical examinations. Conclusion : These results suggest that Rubiae Radix pharmacopuncture might be an effective method to treat patients with knee joint pain. But, It's necessary to have more observations and experiments with Rubiae Radix pharmacopuncture.

• Journal of Acupuncture Research
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• 제28권4호
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• pp.37-47
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• 2011

Objective : The purpose of this study is to evaluate the effect of sweet bee venom pharmacopuncture on the post-stroke hemiplegic shoulder pain. Methods : 40 patients with post-stroke hemiplegic shoulder pain were randomly divided into 2 groups : study group(sweet bee venom pharmacopuncture treatment group, n=21) and Control group(normal saline treatment group, n=19). They were monitored for 4 weeks, followed up with visual analog scale(VAS), pain rating score(PRS), painless passive shoulder range of movement(PROM) and Fugl-Meyer Motor assessment(FMMA) at before treatment(T0), after 2 weeks(T2) and after 4 weeks(T4). Results : 1. Study group and control group showed pain decrease, but study group showed more significant effectiveness in VAS and PRS than control group. 2. Although there is no significant difference in PROM between study group and control group, both groups showed increase of PROM, and study group had some effectiveness on abduction and flexion as the treatment progresses. 3. There is no significant difference in FMMA between study group and control group. Conclusions : This study suggests that sweet bee venom pharmacopuncture has significant analgesic effect on the post-stroke hemiplegic shoulder pain. And it seems that sweet bee venom pharmacopuncture can be applicable to improve PROM in hemiplegia patients with stroke. Further studies based on larger population and long term follow-up are needed to confirm this suggestion.

• Journal of Acupuncture Research
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• 제29권5호
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• pp.31-37
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• 2012

Objectives : The purpose of this study is to evaluate the effect of BUM pharmacopuncture and Soyeom pharmacopuncture on postauricular pain accompanied peripheral facial paralysis. Methods : We observed 31 peripheral facial paralysis patients with postauricular pain at Oriental Hospital of Semyung University from Jan. 1st, 2010 to Feb. 29th, 2012. One group(group A) was treated BUM Pharmacopuncture in the posterior ear with general oriental medical treatment and the other(group B) was treated Soyeom pharmacopuncture in the posterior ear with general oriental medical treatment. Results : 1. Postauricular pain was significantly decreased in each group but no significant difference between two groups. 2. Facial paralysis was significantly improved in each group and significant difference in group B. Conclusions : In peripheral facial paralysis patients with postauricular pain, both BUM pharmacopuncture and Soyeom pharmacopuncture were effective on postauricular pain.

• 대한약침학회지
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• 제17권3호
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• pp.62-69
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• 2014

Objectives: The purpose of this study is to report a case series of advanced cancer patients whose cancer pain was relieved by using autonomic nerve pharmacopuncture (ANP) treatment. ANP is a subcutaneous injection therapy of mountain ginseng pharmacopuncture (MGP) along the acupoints on the spine (Hua-Tuo-Jia-Ji-Xue; 0.5 cun lateral to the lower border of the spinous processes of vertebrae) to enhance the immune system and to balance autonomic nerve function. Methods: Patients with three different types of cancer (gastric cancer, lung cancer, colon cancer with distant metastases) with cancer pain were treated with ANP. 1 mL of MGP was injected into the bilateral Hua-Tuo-Jia-Ji-Xue on the T1-L5 sites (total 12-20 mL injection) of each patient's dorsum by using the principle of symptom differentiation. During ANP treatment, the visual analogue scale (VAS) for pain was used to assess their levels of cancer pain; also, the dosage and the frequency of analgesic use were measured. Results: The cancer pain levels of all three patients improved with treatment using ANP. The VAS scores of the three patients decreased as the treatment progressed. The dosage and the frequency of analgesics also gradually decreased during the treatment period. Significantly, no related adverse events were found. Conclusion: ANP has shown benefit in controlling cancer pain for the three different types of cancer investigated in this study and in reducing the dosage and the frequency of analgesics. ANP is expected to be beneficial for reducing cancer pain and, thus, to be a promising new treatment for cancer pain.

• 척추신경추나의학회지
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• 제6권2호
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• pp.155-164
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• 2011

Objectives : The purpose of this study is to compare the effects of Pharmacopuncture Treatment with Chuna Treatments for Low Back Pain caused by Traffic accidents. Methods : This study was carried out on 81 patients with low back pain caused by Traffic accidents. The patients were divided into 2 group ; The pharmacopuncture(A) group was treated by pharmacopuncture treatment. The chuna(B) group was treated by chuna treatment. We measured Oswestry Disability Index(ODI) and Visual Analog Scale(VAS) on the patients before the treatment and on the 2nd, and 4th weeks after the treatments. Results : After being treated by our methods, the ODI and VAS score's were improved after the 4th weeks treatment in both groups. There is no significant difference on ODI and VAS score after the 4th weeks treatment in both groups. In the early stages(from pre to 2 weeks worth of treatment), Group A showed a decreasing VAS score compared to Group B. In the final stages(from 2 weeks to 4 weeks worth of treatment), Group B showed a higher decreasing amount compared to Group A in VAS score. Conclusions : The results suggest that both pharmacopuncture treatment and chuna treatment is considered to be effective and useful on low back pain caused by traffic accidents. There is no significant difference between pharmacopuncture treatment and chuna treatment for low back pain caused by traffic accidents, however the early stages of treatments(from pre to 2 weeks treatement) show that pharmocopouncture treatment is more effective than chuna treatment for low back pain by traffic accidents. In the final stages(from 2 weeks to 4 weeks treatment), chuna treatment is more effective than pharmacopuncture treatment for low back pain by traffic accident.

• 한방재활의학과학회지
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• 제28권2호
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• pp.83-89
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• 2018

Objectives The purpose of this study is to compare the effectiveness of Hwangryunhaedok-tang Pharmacopuncture, Bee Venom Pharmacopuncture and Jungsongouhyul Pharmacopuncture on cervical pain caused by traffic accident retrospectively. Methods The retrospective observational chart review was conducted to 52 cases of traffic accident patients who had admitted to Daegu haany University Pohang Korean Hospital from December 1st, 2015 to May 31th, 2017. The patients were classified into three groups by which pharmacopuncture was used as Hwangryunhaedoktang pharmacopuncture treatment group, bee venom pharmacopuncture treatment group and Jungsongouhyul pharmacopuncture treatment group. Additionally, outcomes should include Neck disability index(NDI) and patient's global assessment(PGA) for evaluate the treatment effects. Results Each pharmacopuncture treatment group showed significant decrease of NDI and PGA. But there was no significant difference in NDI and PGA between three groups. Conclusions According to the results, we found out that 3 kinds of pharmacopunctures are considered to be effective and useful for cervical pain patients caused by traffic injury. Further well designed prospective studies are needed.