• Mycobiology
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• 제50권5호
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• pp.374-381
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• 2022

In the mating of filamentous basidiomycetes, dikaryotic mycelia are generated through the reciprocal movement of nuclei to a monokaryotic cytoplasm where a nucleus of compatible mating type resides, resulting in the establishment of two different dikaryotic strains having the same nuclei but different mitochondria. To better understand the role of mitochondria in mushrooms, we created four sets of dikaryotic strains of Lentinula edodes, including B2×E13 (B2 side) and B2×E13 (E13 side), B5×E13 (B5 side) and B5×E13 (E13 side), E8×H3 (E8 side) and E8×H3 (H3 side), and K3×H3 (K3 side) and K3×H3 (H3 side). The karyotypes and mitochondrial types of the dikaryotic strains were successfully identified by the A mating type markers and the mitochondrial variable length tandem repeat markers, respectively. Comparative analyses of the dikaryotic strains on the mycelial growth, substrate browning, fruiting characteristics, and mitochondrial gene expression revealed that certain mitochondria are more effective in the mycelial growth and the production of fruiting body, possibly through the activated energy metabolism. Our findings indicate that mitochondria affect the physiology of dikaryotic strains having the same nuclear information and therefore a selection strategy aimed at mitochondrial function is needed in the development of new mushroom strain.

• Geomechanics and Engineering
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• 제32권3호
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• pp.245-254
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• 2023

Through qualitative analysis and quantitative analysis, the contradictory conclusions about the stability of the settled goaf with two-layer coal seams subject to building load were obtained. Therefore, it is necessary to combine the additional stress method and numerical simulation to further analyze the foundation stability. Through borehole analysis and empirical formula analogy, the height of water-conducting fracture zone in No.4 coal and No.9 coal were obtained, providing the calculation range of water-conducting fracture zone for numerical simulation. To ensure the accuracy of the elastic modulus of broken gangue, the stress-strain curve were obtained by broken gangue compression test in dried state of No.4 coal seam and in soaking state of No.9 coal seam. To ensure the rationality of the numerical simulation results, the actual measured subsidence data were retrieved by numerical simulation. FISH language was used to analyze the maximum building load on the surface and determine the influence depth of building load on the foundation. The critical building load was 0.16 MPa of No.4 settled goaf and was 1.6 MPa of No.9 settled goaf. The additional stress affected the water-conducting fracture zone obviously, resulted in the subsidence of water-conducting fracture zone was greater than that of bending subsidence zone. In this paper, the additional stress method was analyzed by numerical simulation method, which can provide a new analysis method for the treatment and utilization of the settled goaf.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2001년도 추계학술대회
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• pp.39-45
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• 2001

Extremophiles are unique microorganisms that are adapted to survive in ecological niches such as high or low temperatures, extremes of pH, high salt concentrations and high pressure. These unusual microorganisms have unique biochemical features which can be exploited for use in the biotechnological industries. Due to the high biodiversity of extremophilic archaea and bacteria and their existence in various biotopes a variety of biocatalysts with different physicochemical properties have been discovered. The extreme molecular stability of their enzymes, membranes and the synthesis of unique organic compounds and polymers make extremophiles interesting candidates for basic and applied research. Some of the enzymes from extremophiles, especially hyperthermophilic marine microorganisms (growth above $85^{\circ}C$), have already been purified in our laboratory. These include the enzyme systems from Pyrococcus, Pyrodictium, Thermococcus and Thermotoga sp. that are involved in polysacharide modification and protein bioconversion. Only recently, the genome of the thermoalkaliphilic strain. Anaerobranca gottschalkii has been completely sequenced providing a unique resource of novel biocatalysts that are active at high temperature and pH. The gene encoding the branching enzyme from this organism was cloned and expressed in a mesophilic host and finally characterized. A novel glucoamylase was purified from an aerobic archaeon which shows optimal activity at $90^{\circ}C$ and pH 2.0. This thermoacidophilic archaeon Picrophilus oshimae grows optimally at pH 0.7 and $60^{\circ}C$. Furthermore, we were able to detect thermoactive proteases from two anaerobic isolates which are able to hydrolyze feather keratin completely at $80^{\circ}C$ forming amino acids and peptides. In addition, new marine psychrophilic isolates will be presented that are able to secrete enzymes such as lipases, proteases and amylases possessing high activity below the freezing point of water.

• 한국지진공학회논문집
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• 제27권2호
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• pp.77-82
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• 2023

The structural analysis module is an essential part of any integrated structural system. Diverse integrated systems today require, from the analysis module, efficient real-time responses to real-time input such as earthquake signals, extreme weather-related forces, and man-made accidents. An integrated system may also be for the entire life span of a civil structure conceived during the initial conception, developed throughout various design stages, effectively used in construction, and utilized during usage and maintenance. All these integrated systems' essential part is the structural analysis module, which must be automated and computationally efficient so that responses may be almost immediate. The finite element method is often used for structural analysis, and for automation, many effective finite element meshes must be automatically generated for a given analysis. A computationally efficient finite element mesh generation scheme based on the r-h method of mesh refinement using strain deviations from the values at the Gauss points as error estimates from the previous mesh is described. Shape factors are used to sort out overly distorted elements. A standard cantilever beam analyzed by four-node plane stress elements is used as an example to show the effectiveness of the automated algorithm for a time-domain dynamic analysis. Although recent developments in computer hardware and software have made many new applications in integrated structural systems possible, structural analysis still needs to be executed efficiently in real-time. The algorithm applies to diverse integrated systems, including nonlinear analyses and general dynamic problems in earthquake engineering.

• Structural Engineering and Mechanics
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• 제86권1호
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• pp.1-16
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• 2023

The free vibration of temperature-dependent functionally graded plates (FGPs) resting on a viscoelastic foundation is investigated in this paper using a newly developed simple first-order shear deformation theory (FSDT). Unlike other first order shear deformation (FSDT) theories, the proposed model contains only four variables' unknowns in which the transverse shear stress and strain follow a parabolic distribution along the plates' thickness, and they vanish at the top and bottom surfaces of the plate by considering a new shape function. For this reason, the present theory requires no shear correction factor. Linear steady-state thermal loads and power-law material properties are supposed to be graded across the plate's thickness. Uniform, linear, non-linear, and sinusoidal thermal rises are applied at the two surfaces for simply supported FGP. Hamilton's principle and Navier's approach are utilized to develop motion equations and analytical solutions. The developed theory shows progress in predicting the frequencies of temperature-dependent FGP. Numerical research is conducted to explain the effect of the power law index, temperature fields, and damping coefficient on the dynamic behavior of temperature-dependent FGPs. It can be concluded that the equation and transformation of the proposed model are as simple as the FSDT.

• 한국지반공학회논문집
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• 제22권9호
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• pp.23-36
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• 2006

본 연구는 사질토 지반에 근입되어 있는 벽체의 극한지지력을 구하기 위해 새로운 근사적인 해석법의 전개과정에 대해 설명한다. 이러한 근사적인 형태의 해석기법은 상계 및 하계법으로 구성되어 있다. 상 하계법으로 계산된 값은 소성영역에서 구해진 2차원 실내벽체모형의 하중재하시험 및 유한요소해석 결과와 비교하였다. 비교 결과, 모형실험과 유한요소해석으로부터 구한 극한하중 값은 상계와 하계 사이에 모두 분포하는 것으로 나타났다. 이러한 비교에서 특이 할 사항은 하계법으로 구한 벽체의 극한하중이 모형실험 및 유한요소해석에서 구한 극한하중과 잘 일치되는 것을 보여 주었다. 그러나, 평면변형률 조건에서 기존의 경험적인 식에 의한 계산에서 얻어진 극한하중은 하계법의 극한하중에 훨씬 못 미치는 것으로 나타났다.

• 한국지반공학회논문집
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• 제22권11호
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• pp.25-35
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• 2006

본 연구에서는 폐어망을 이용한 보강 경량토의 역학적 특성을 고찰하였다. 본 실험에서 사용된 보강 경량토는 부산 신항 건설현장에서 채취한 준설토와 시멘트, 기포 및 폐어망으로 구성되어졌다. 다양한 함유율의 폐어망이 혼합된 보강 경량토의 보강효과를 고찰하고, 무보강 경량토와 보강 경량토의 거동 특성을 비교하기 위하여 다양한 실내실험이 수행되어졌다. 보강 경량토에 대한 실험결과 응력-변형 관계와 일축압축강도는 폐어망에 의해 큰 영향을 받는다는 것이 나타났다. 보강 경량토의 압축강도는 양생기간이 증가할수록 증가하며, 폐어망을 첨가함으로써 일반적으로 증가하지만, 압축강도 증가량은 폐어망 함유율에 비례하지는 않는다. 본 실험의 경우 폐어망 함유율이 0.25%일 때 최대 압축강도가 발현되는 것을 알 수 있었다. 한편, 보강 경량토의 함수비 변화는 양생기간 7일까지 급격하게 감소한 후 일정한 값에 수렴하였다.

• 한국지반공학회논문집
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• 제23권12호
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• pp.95-107
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• 2007

본 연구에서는 국내 서 남해안의 해성점토를 대상으로 대형블럭시료와 피스톤 시료를 채취하여 시료교란도 평가와 다양한 압밀시험을 수행하였다. 이들 시료에 대하여 시료교란도 분석과 다양한 압밀시험결과를 토대로 압축특성을 규명하였다. 그 결과, 대형블럭시료는 압밀시험으로 결정한 선행압밀하중까지의 체적변형률이 피스톤 시료보다 더 낮으며, 대형블럭시료에서 얻은 압축지수는 피스톤시료의 압축지수에 비하여 더 크게 나타났다. 샘플링 방법에 따른 압축지수에 끼치는 영향을 고려하여 새로운 압축지수 추정식을 제안하였다. 이러한 압축지수 제안식을 이용하여 압밀침하량을 추정한다면, 관행적으로 피스톤 샘플로부터 압축특성을 결정하는 경우보다 더 합리적으로 침하량을 예측할 수 있을 것으로 사료된다.

• Computers and Concrete
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• 제31권4호
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• pp.349-358
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• 2023

This paper describes an in-depth analysis on flexural strength of a girder-deck system experiencing a strand debonding damage with various strengthening systems, based on finite element software ABAQUS. A detailed finite element analysis (FEA) model was developed and verified against the relevant experimental data performed by other researchers. The proposed analytical model showed a good agreement with experimental data. Based on the verified FE model, over a hundred girder-deck systems were investigated with the consideration of following variables: 1) debonding level, 2) span-to-depth ratio (L/d), 3) strengthening type, 4) strengthening material thickness. Based on the data above, a new detailed analytical model was developed and proposed for estimating residual flexural strength of the strand-debonding damaged girder-deck system with strengthening systems. It was demonstrated that both finite element model and analysis model could be used to predict flexural behaviors for debonding damaged prestressed girder-deck systems. Since the strands are debonding from surrounding concrete over a certain zone over the length of the beam, the increase of strain in strands can be linked with a ratio ψ, which is L_p/c. The analytical model was proposed and developed regarding the ratio ψ. By conducting procedure of calculating ψ, the ψ value varies from 9.3 to 70.1. Multiple nonlinear regression analysis was performed in Software IBM SPSS Statistics 27.0.1 to derive equation of ψ. ψ equation was curved to be an exponential function, and the independent variable (X) is a linear function in terms of three variables of debonding level (λ), span length (L), and amount of strengthening material (A_s). The coefficient of determinate (R²) for curve fitting in nonlinear regression analysis is 0.8768. The developed analytical model was compared to the ultimate capacities computed by FEA model.

• Steel and Composite Structures
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• 제46권6호
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• pp.793-804
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• 2023

In this study, cyclic loading tests were conducted to assess the seismic performance of cast-in-place (CIP) concrete-filled hollow core precast concrete columns (HPCC) constructed using steel ducts and rubber tubes. The outer shells of HPCC, with a hollow ratio of 47%, were fabricated using steel ducts and rubber tubes, respectively. Two combinations of shear studs & long threaded bars or cross-deformed bars & V-ties were employed to ensure the structural integrity of the old concrete (outer shell) and new CIP concrete. Up to a drift ratio of 3.8%, the hysteresis loop, yielding stiffness, dissipated energy, and equivalent damping ratio of the HPCC specimens were largely comparable to those of the solid columns. Besides the similarities in cyclic load-displacement responses, the strain history of the longitudinal bars and the transverse confinement of the three specimens also exhibited similar patterns. The measured maximum moment exceeded the predicted moment according to ACI 318 by more than 1.03 times. However, the load reduction of the HPCC specimen after reaching peak strength was marginally greater than that of the solid specimen. The energy dissipation and equivalent damping ratios of the HPCC specimens were 20% and 25% lower than those of the solid specimen, respectively. Taking into account the overall results, the structural behavior of HPCC specimens fabricated using steel ducts and rubber tubes is deemed comparable to that of solid columns. Furthermore, it was confirmed that the two combinations for securing structural integrity functioned as expected, and that rubber air-tubes can be effectively used to create well-shaped hollow sections.