• Journal of Korean Society of Steel Construction
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• v.22 no.4
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• pp.389-398
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• 2010

The flexural strength of composite HSB I-girders under a positive moment was investigated using the moment-curvature analysis method to evaluate the applicability of the current AASHTO LRFD design specifications to such girders. A total of 2,391 composite I-girder sections that satisfied the section proportion limits of the AASHTO LRFD specifications was generated by the random sampling technique to consider a wide range of section properties. The flexural capacities of the sections were calculated inthe nonlinear moment-curvature analysis in which the HSB600 and HSB800 steels were modeled as an elasto-plastic strain-hardening material, and the concrete, as a CEB-FIP model. The effects of the ductility ratio and the compressive strength of the concrete slab on the flexural strength of the composite girders made of HSB and SM520-TMC steels were analyzed. The numerical results indicated that the current AASHTO LRFD equation can be used to calculate the flexural strength of composite girders made of HSB600 steel. In contrast, the current AASHTO LRFD equation was found to be non-conservative in its prediction of the flexural strength of composite HSB800 girders. Based on the numerical results of this study for 2,391 girders, a new design equation for the flexural strength of composite HSB800 girders in a positive moment was proposed.

• Journal of the Korean Geotechnical Society
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• v.19 no.4
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• pp.145-153
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• 2003

The resonant column testing determines the shear modulus and material damping factor dependent on the shear strain magnitude, based on the wave-propagation theory. The determination of the dynamic soil properties requires the theoretical formulation of the dynamic behavior of the resonant column testing system. One of the theoretical formulations is the use of the wave equation for the soil specimen in the resonant column testing device. Wood, Richart and Hall derived the wave equation by assuming the linear elastic soil, and didn't take the material damping into consideration. Hardin incorporated the viscoelastic damping of soil in the wave equation, but he had to assume the material damping factor for the determination of the shear modulus. For the better theoretical formulation of the resonant column testing, this study derived a new wave equation to include the viscosity of soil, and proposed an approach for the solution. Also, in this study, the equation of motion for the testing system, which is another approach of the theoretical formulation of the resonant column testing, was also derived. The equation of motion leads to the better understanding of the resonant column testing, which includes the dynamic magnification factor and the phase angle of the response. For the verification of the proposed equation of motion for the resonant column testing, the finite element analysis was performed for the resonant column testing. The comparison of the dynamic magnification factors and the phase angles far the system response were performed.

• Journal of the Korean GEO-environmental Society
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• v.10 no.5
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• pp.19-26
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• 2009

Rammed Aggregate Pier (RAP) method is intermediate foundation between deep and shallow foundation, and it has been built in world wide. RAP represents a relatively new method that has grown steadily over 19 years since Geopier of USA developed this revolutionary method in 1989. The investigation and research in domestic is not accomplished. In this paper, the examined details of different spacing of piles, bearing capacities, respectively, conclude with recommendations on how RAP can be used in future needs. This documentation further provides comparisons of the laboratory test results which were obtained from changing the spacing of piles, namely installed rammed aggregate pier. Laboratory model test was administered in a sand box. Strain control test was conducted to determine the bearing capacities of the piers; 20 mm, 30 mm and 40 mm RAP in diameter using drilling equipment to make holes were installed in sand at initial relative densities of 40%. By comparing different spacing of piles, in this experiment, piles are spaced structually span, form a ring shape, narrowing the distance of each other, to the center. the result shows that as diameter of pier is bigger in diameter, bearing capacity also dramatically increased due to raised stiffness. Also, as the space between each piers was closed, the settlement rate of soil was decreased significantly. From the test results, as the space between each piles were getting closer, it allows greater chances to have more resistance to deformation, and shows more improved stability of structures. After from the verification work which is continuous leads the accumulation of the site measuring data which is various, and bearing capacity and the settlement is a plan where the research will be advanced for optimum installed RAP.

• Composites Research
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• v.13 no.5
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• pp.50-59
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• 2000

In this paper, two methods to suppress flutter of the composite panel are examined. First, in the active control method, a controller based on the linear optimal control theory is designed and control input voltage is applied on the actuators and a PZT is used as actuator. Second, a new technique, passive suppression scheme, is suggested for suppression of the nonlinear panel flutter. In the passive suppression scheme, a shunt circuit which consists of inductor-resistor is used to increase damping of the system and as a result the flutter can be attenuated. A passive damping technology, which is believed to be more robust suppression system in practical operation, requires very little or no electrical power and additional apparatuses such as sensor system and controller are not needed. To achieve the great actuating force/damping effect, the optimal shape and location of the actuators are determined by using genetic algorithms. The governing equations are derived by using extended Hamilton's principle. They are based on the nonlinear von Karman strain-displacement relationship for the panel structure and quasi-steady first-order piston theory for the supersonic airflow. The discretized finite element equations are obtained by using 4-node conforming plate element. A modal reduction is performed to the finite element equations in order to suppress the panel flutter effectively and nonlinear-coupled modal equations are obtained. Numerical suppression results, which are based on the reduced nonlinear modal equations, are presented in time domain by using Newmark nonlinear time integration method.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.265-271
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• 1985

The classification of bacteriophage could be followed by several criteria. In this study three criteria were used for classification of Lactobacillus casei bacteriophag. In serological classification. antiserum was prepared by rabbit and used for classification. The inactivation effect of phage by antiserum was exponential and L. casei phage was classified in to three serological groups by inactivation rate (K-values). The Lac Y group was proved as a new serological group but the Lac J and Lac S group were shown the same results as previous reports. From the comparison of restriction enzyme pattern of phage DNA, Lac J group was divided into four sub-groups. According to the difference of host range, Lac J-II group was further subdivided into three groups. These results were shown that L. casei strains S-1 bacteriophage was classified into 8 sub-groups. The phage YK of Lac Y group was shown to consist of a icosahedral head about 95nm in diameter, a contractile tail about 150nm in length and 20nm in width. The tail of YK phage is composed of stacked disks(4nm repeat)and a hexagonal baseplate. The molecular weight of YK phage DNA was approximately 85.6 Mdalton.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.48 no.10
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• pp.773-782
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• 2020

The development of joint FE models for deep learning neural network (DLNN)-based hybrid FEA is presented. Material models of bolts and bearings in the front axle of tractor, showing complex behavior induced by various tightening conditions, were replaced with DLNN models. Bolts are modeled as one-dimensional Timoshenko beam elements with six degrees of freedom, and bearings as three-dimensional solid elements. Stress-strain data were extracted from all elements after finite element analysis subjected to various load conditions, and DLNN for bolts and bearing were trained with Tensorflow. The DLNN-based joint models were implemented in the ABAQUS user subroutines where stresses from the next increment are updated and the algorithmic tangent stiffness matrix is calculated. Generalization of the trained DLNN in the FE model was verified by subjecting it to a new loading condition. Finally, the DLNN-based FEA for the front axle of the tractor was conducted and the feasibility was verified by comparing with results of a static structural experiment of the actual tractor.

• The Korean Journal of Mycology
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• v.19 no.4
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• pp.266-275
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• 1991

The gene CDC70 encoding the${\alpha}-subunit$ of G protein has been known to be a component involved in mating pheromone signalling in the yeast, Saccharomyces cerevisiae. To isolate mutations of the genes involved in the signal transduction, Saccharomyces cerevisiae the strain bearing the cdc70-5 mutation was mutagenized to be forced to recover the ability of colony-formation at restrictive temperature, which means the new mutation can suppress the temperature sensitivity of the cdc70-5 phenotypes. Among these suppressors, $sir^-$ and $mat{\alpha}2^{-}$ mutations are excluded because of no relationship to signal transducer. And the selected suppressors were analyzed for the linkage relationships by the tetrad analysis. Out of fifteen suppressors isolated, twelve were classified into four linkage groups, designated as sga1, sga2, sga3, sga4 by the tetrad analysis. The other three genes were determined for the linkage.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.3
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• pp.10-17
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• 2016

This paper presents a laboratory validation of a new approach for Finite Element Model Updating(FEMU) on short-span bridges by combining ambient vibration measurements with static load input-deflection output measurements. The conventional FEMU approach based on modal parameters requires the assumption on the system mass matrix for the eigen-value analysis. The proposed approach doesn't require the assumption and even provides a way to update the mass matrix. The proposed approach consists of two steps: 1) updating the stiffness matrix using the static input-deflection output measurements, and 2) updating the mass matrix using a few lower natural frequencies. For a validation of the proposed approach, Young's modulus of the laboratory model was updated by the proposed approach and compared with the value obtained from strain-stress tests in a Universal Testing Machine. Result of the conventional FEMU was also compared with the result of the proposed approach. It was found that proposed approach successfully estimated the Young's modulus and the mass density reasonably while the conventional FEMU showed a large error when used with higher-modes. In addition, the FE modeling error was discussed.

• Journal of Microbiology and Biotechnology
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• v.28 no.7
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• pp.1122-1132
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• 2018

In this study, we attempted to find new and efficient microbial enzymes for producing rare sugars. A ribose-5-phosphate isomerase B (OsRpiB) was cloned, overexpressed, and preliminarily purified successfully from a newly screened Ochrobactrum sp. CSL1, which could catalyze the isomerization reaction of rare sugars. A study of its substrate specificity showed that the cloned isomerase (OsRpiB) could effectively catalyze the conversion of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose, which was unconventional for RpiB. The optimal reaction conditions ($50^{\circ}C$, pH 8.0, and 1 mM $Ca^{2+}$) were obtained to maximize the potential of OsRpiB in preparing $\text\tiny{L}$-rhamnulose. The catalytic properties of OsRpiB, including $K_m$, $k_{cat}$, and catalytic efficiency ($k_{cat}/K_m$), were determined as 43.47 mM, $129.4sec^{-1}$, and 2.98 mM/sec. The highest conversion rate of $\text\tiny{L}$-rhamnose under the optimized conditions by OsRpiB could reach 26% after 4.5 h. To the best of our knowledge, this is the first successful attempt of the novel biotransformation of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose by OsRpiB biocatalysis.

• Research in Plant Disease
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• v.15 no.3
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• pp.222-229
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• 2009

A Pseudomonas strain SG3 producing biosurfactant and showing antifungal and insecticidal activities was isolated from agricultural soil severely contaminated with machine oils. The antagonistic bacterium inhibited mycelial growth of all of the tested fungal pathogens. The fermentation broth of SG3 also effectively suppressed the development of various plant diseases including rice blast, tomato gray mold, tomato late blight, wheat leaf rust, barley powdery mildew and red pepper anthracnose. An antifungal substance was isolated from the fermentation broth of SG3 by ethyl acetate partitioning, silica gel column chromatography and preparative HPLC under the guide of bioassay. The chemical structure of the antifungal substance was determined to be rhamnolipid B by mass and NMR spectral analyses. The antifungal biosurfactant showed a potent in vivo antifungal activity against gray mold and late blight on tomato plants. In addition, rhamnolipid B inhibited mycelial growth of B. cinerea causing tomato gray mold and zoospore germination and mycelial growth of P. infestans causing tomato late blight. Pseudomonas sp. SG3 producing rhamnolipid B could be used as a new biocontrol agent for the control of plant diseases occurring on tomato plants.