• 한국의류학회지
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• 제34권5호
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• pp.866-872
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• 2010

This study investigates the methods of the natural dyeing of silk fabrics with Machilus thunbergii cortex extract. After the dyeing of silk with a Machilus thunbergii cortex extract, the dyeability of the Machilus thunbergii cortex extract was evaluated with the dyeing time, concentration, temperature, the numbers of repeated dyeing, the pH of the dyebath, the changes of the K/S value, and surface colors by the methods of mordanting and color fastness. The effective dyeing conditions with silk fabrics were at a concentration of 120g/L, the dyeing temperature at $80^{\circ}C$, and the dyeing time for one hour ten minutes. The effective number of repeated dyeing was three times. The dyeing operation was carried out in a neutral dyebath of pH 7. The K/S value was higher in most of the pre-mordants (except the Sn mordant) and a high K/S value was shown in the copper pre-mordant. The colors of the silk fabrics with Machilus thunbergii cortex were of various brown shades. The color difference was distinct when using the Fe-mordant and the colorfastness of all the dyed samples was low; however, the dry cleaning fastness was excellent at the 4-5 grade.

• 한국자동차공학회논문집
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• 제20권6호
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• pp.61-66
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• 2012

Conventional method to transform vehicle driving condition to engine operation mode is to use vehicle road load under neutral gear and mechanical efficiency of drivetrain. But this method requires additional measurement of efficiency of drivetrain on a test rig. And this measurement is normally done at fixed speed and thus estimated accuracy of engine operation mode is not considered to be high enough. This study suggests new method to calculate engine operation mode for prescribed driving mode such as NEDC using vehicle coastdown test under gear engaged condition without measurement of mechanical efficiency of drivetrain. Coastdown test was done under neutral and gear engaged condition for comparison and also trial to extract mechanical loss of drivetrain was carried out. Calculated engine torque by conventional and newly suggested method was compared with actually measured torque of a vehicle on a chassis dynamometer during NEDC. Newly suggested method showed slightly higher accuracy of accumulated brake work during NEDC.

• 한국식품과학회지
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• 제36권4호
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• pp.643-647
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• 2004

땅콩껍질에 함유된 항미생물 활성물질의 탐색을 위해 땅콩껍질을 MeOH로 추출하고 이 MeOH 추출물을 용매분획하여 EtOAc 중성획분을 얻었다. 이 획분에 함유된 항미생물 활성물질을 silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, ODS column chromatography로 정제한 다음 HPLC를 이용하여 1종의 화합물을 단리하였다. $^1H-NMR$, MS 및 NOESY 분석에 의해 땅콩껍질로부터 분리된 항미생물 활성물질은 pratensein으로 동정되었다.

• 한국미생물·생명공학회지
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• 제17권5호
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• pp.407-411
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• 1989

Oxytetracycline 생산균주인 Streptomyces rimosus를 maltose 2%, NH$_4$Cl 0.5%, yeast extract 0.4 %, MGSO$_4$.7$H_2O$ 0.2% 조성의 배지를 배양초기 PH 6.5로 하여 3$0^{\circ}C$, 72시간 진탕배양하여 얻은, 세포외 protease를 유안분획, Sephadex A-50 이온교환, Sephadex G-100 gel 여과를 행하여 정제하였다. 효소의 최적온도는 5$0^{\circ}C$이며, 최적 pH는 8.0이었으며, Co$^{2+}$ 이온에 의해 활성화되며 Hg$^{2+}$, Fe$^{2+}$ 및 EDTA에 의해 저해를 받으며 casein 분자량을 23,600으로 추정하여 구한 Km값은 2.7$\times$$10^{-4}$M이었다.

• Journal of Pharmaceutical Investigation
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• 제18권3호
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• pp.125-131
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• 1988

This study was undertaken to investigate the proteolytic enzyme from Neungee mushroom [Sarcodon aspratus (Berk) S. Ito]. The proteolytic activity of Neungee was higher than other several edible mushrooms under various pHs. The potency of proteolytic enzyme of Neungee was same as the digestive drugs containing protease. So the proteolytic activity of the enzyme was increased in neutral or weak alkaline pH, whose characteristics would be alkaline protease. The specific activity of the purified enzyme obtained by using Tris acryl CM-cellulose ion exchange increased 20 times as compared with that of the crude extract. The proteolytic enzyme was stable at room temperature, but decomposition was fast when incubated at higher temperature more than $40^{\circ}C$. The half life of the enzyme was longest in neutral pH and rate constant was increased in acidic or alkaline solution.

• Journal of Ginseng Research
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• 제14권3호
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• pp.404-415
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• 1990

This study is conducted to evalute the effect of ginseng fraction component (ginseng extract solution, GES; ginseng protein, GP; ginseng saponin, GSA; ginseng residue, GR) upon hyperlipidemia and fatty liver induced by high fat administration. In doing so, the serum, liver and epididymal adpoid tissue have been examined for lipid components level and lipoprotein fraction. Feces bile acid and neutral sterol excretion have been also measured. 1'he results obtained from this study are as follows. 1. Serum, liver, epididymal lipid components of GP and GSA group were significantly lower than the controlgroup. 2. During the feeding experiment, VLDL and LDL increase while HDL decrease in all group. However the degree of VLDL and LDL increase and HDL decrease were signficantly small in GP and GSA group compared with control group. 3. In the excretion of bile acid and neutral sterol, all experiment group showed increased excretion in the comparison of control group.

• 한국식품영양과학회지
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• 제27권3호
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• pp.381-385
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• 1998

Chemical components of Perillar semen and physico-chemical properties o Perillae semen oil were analyzed for the use as an edible oil. The proximate compositions of Perillae semen were 7.5% moisture, 33.2% crude fat, 16.3% crude protein, 2.8% crude ash, 6.5% crude fiber, and 33.7% nitrogen free extract. The major amino acids of Perillae semen were glutamic acid(66.9mg%), aspartic acid (32.5mg%), histidine(21.6mg%), and phenylaanine (20.1mg%). The ratio of essential/total amino acid was 41.3%. The physico-chemical properties of the seed oil were 0.915 specific gravity, 1.4808 refractive index, 3.6 acid value, 181.7 iodine value, and 194.0 saponification value. Composition of major lipid of the oil fractionated by silicic acid chromatography was 94.2% neutral lipids and 5.8% polar lioids. The major fatty acids of the oil were linolenic, linoleic and oleic acid. Neutral lipids consisted of 59.9% linolenic acid, 15.6% oleic acid, 6.6% palmitic acid, and 2.5% stearic acid. Polar lipids consisted of 58.5% linolenic acid, 18.1% linoleic acid, 12.7% oleic acid, 7.7% palmitic acid, and 3.0% stearic acid.

• Food Science and Biotechnology
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• 제18권2호
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• pp.323-329
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• 2009

The prescriptions (DB-1-DB-5) were prepared with the active herbal medicines, Acanthopanax senticosus, Glycyrrhiza uralensis, Polygonatum odoratum, and Cichorium intybus. The most active crude polysaccharide fraction (DB-2-3), which was isolated through the fractionation of hot-water extract from DB-2, was significantly reduced by periodate oxidation (52.7 and 63.7%) on intestinal immune system modulating and anticancer activity. When DB-2-3 was further fractionated by column chromatographies, DB-2-3IIc-2 showed the most potent activities. In addition, DB-2-3IIc stimulated the proliferation of bone marrow cells via Peyer's patch in dose-dependent pattern by oral administration. The metastasis of colon 26-M3.1 lung carcinoma had significantly inhibited in mice fed DB-2-3IIc at 1 mg/mouse (43.8%). DB-2-3IIc-2 mainly contained uronic acid (46.1%) and 42.5% of neutral sugar with a small amount of protein (7.6%), and component sugar analysis also showed that DB-2-3IIc-2 was composed Ara, Gal, and GalA (molar ratio; 0.50:0.63:1.00). It may be suggested that activities of DB-2-3IIc-2 are resulted from pectic polysaccharides containing a polygalacturonan moiety with side chain of neutral sugars, such as Ara and Gal.

• Animal Bioscience
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• 제34권6호
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• pp.1014-1021
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• 2021

Objective: The aim of this study was to determine the effect of dietary ground licuri on lamb performance. Methods: Forty male lambs were used in a completely randomized design to test the effects of 0, 5, 10, and 15 g/kg of ground licuri added to diets. The trial lasted for 75 days. Intake, digestibility, physically effective neutral detergent fiber, and chewing activity were estimated. Blood samples were taken on day 45 to determine the concentrations of glucose, urea, non-esterified fatty acids, and triglycerides. Average daily gain (ADG) were determined on the last day of the experimental trial. Results: Licuri inclusion markedly increased dietary neutral detergent fiber and ether extract (EE) content, but it decreased dry matter (DM) intake. However, the intake and digestibility of EE linearly increased. The ADG decreased linearly (p<0.05) with licuri inclusion. Licuri had no effect (p>0.05) on the concentrations of blood metabolites; however, blood urea increased (p<0.05), while serum glucose decreased (p<0.05). Conclusion: The physically effective fiber of ground licuri is similar to Tyfton hay and licuri inclusion decreases lamb performance due to a decreased in DM intake.

• Asian-Australasian Journal of Animal Sciences
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• 제23권12호
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• pp.1587-1593
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• 2010

Pastures of the Apennines of Central Italy contribute to feed resources of high altitude (above 1,300 m sea level) grazing systems. The objective of this study was to evaluate the effectiveness of faecal extract from the yak (Bos grunnienes) as an alternative microbial inoculum to rumen fluid for estimation of digestibility of several forage species. Forage samples produced at high altitude were tested in this study: four legumes (Lathyrus sativus L., Lotus corniculatus L., Onobrychis viciaefolia L. and Trifolium pratense L.), three forbs (Achillea millefolium L., Potentilla reptans L. and Teucrium flavum L.) and one grass (Brachipodyum pinnatum L.) were incubated with yak rumen fluid or faecal extract. A large variability in chemical composition was observed among the species collected. Rumen liquor and faecal samples were collected from adult healthy yak. The $Daisy^{II}$ incubator was used to evaluate the nutrient digestibility of forages using rumen liquor as control and faecal extract as alternative microbial inoculum sources. Filter bags containing samples of browse species were added to the four digestion vessels along with their respective inoculum and then incubated for 48 h and dry matter (DM), organic matter (OM), crude protein (CP), neutral and detergent fiber (NDF) digestibility was determined. There was a significant relationship between estimates, indicating that faecal liquor has the potential to be used instead of rumen fluid for estimation of in vitro digestibility of plants. It is concluded that the $Daisy^{II}$ incubator results are appropriate for the determination of in vitro digestibility of nutrients using faecal liquor to define the potential for adaptation of yak to new pastures.