한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제17권5호
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- Pages.407-411
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- 1989
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Streptomyces rimosus가 생산하는 Protease의 정제와 특성
Purification and Properties of Extracellular Protease from Streptomyces rimosus
초록
Oxytetracycline 생산균주인 Streptomyces rimosus를 maltose 2%, NH
Extracellular neutral pretense of Streptomyces rimosus producing oxytetracycline was purified by ammonium sulfate fractionation, DEAE Sephadex A-50 chromatography and Sephadex G-100 gel filteration, and was showed single band on the cathodic gel electrophoresis. The optimum pH and temperature of the enzyme were pH 8.0 and 6