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Isolation and Identification of Pratensein with Antimicrobial Activity from the Peanut Shells  

Wee, Ji-Hyang (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
Moon, Jae-Hak (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
Park, Keun-Hyung (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
Publication Information
Korean Journal of Food Science and Technology / v.36, no.4, 2004 , pp. 643-647 More about this Journal
Abstract
Natural antimicrobial substance from peanut (Arachis hypogaea) shells was isolated and structurally elucidated. Peanut shells were extracted with methanol (MeOH) and concentrated in vacuo, MeOH extract was solvent-fractionated with ethyl acetate (EtOAc) and various buffer to obtain EtOAc acidic, neutral, and phenolic fractions. EtOAc neutral fraction, which showed antimicrobial activity, was purified through silica gel adsorption column, Sephadex LH-20 column, ODS column, and high performance liquid chromatographies, and its active substance was isolated and identified as pratensein (3',5,7-trihydroxy-4'-methoxyisoflavone) by spectroscopic methods of proton-nuclear magnetic resonance, mass spectrometry, and nuclear overhauser enhancement spectroscopy.
Keywords
peanut shells; antimicrobial activity; pratensein; isoflavonoid;
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Times Cited By KSCI : 1  (Citation Analysis)
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