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Growth Characteristics , Yield , and Nutritive Value of Early- and Late Maturing Cultivars of Orchargrass ( Dactylis glomerate L. ) (조.만생 Orchargrass 품종의 생육특성과 수량성 및 사료가치 비교 연구)

  • Seo, Sung;Shin, Dong-Eun;Chung, Eui-Soo;Kang, Woo-Sung;Yang, Jong-Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.17 no.1
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    • pp.27-34
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    • 1997
  • This experiment was carried out to determine growth characteristics, dry matter (DM) yield, seasonal yield distribution and nutritive value of early- and late maturity orchardgrass, 1990 to 1992. The cultivars used in this study were Potomac, Hallmark (early maturing cultivar) and Lidacta, Rancho (late maturing cultivar), and a mixture plot (Potomac 25% + Hallmark 25% + Lidacta 25% + Rancho 25%) was involved. The seeding rate was 20 kgha in all treatments. Plant height of Potomac and Hallmark was longer than that of Lidacta and Rancho. The dates of initial heading and 50% heading of early maturing cultivars (Apr. 30 and May 10) were 10 days earlier than those of late matuing cultivars. Potomac and Hallmark were upright, and Lidacta and Rancho were semi-upright type, and severe disease and weeds (30 ~ 40%) were observed at Lidacta plot. On an average of three years, the DM yields of Potomac (10,549 kglna), Hallmark (10,435 kg) and mixture (10,356 kg) were significantly (P<0.05) higher than those of Rancho (9,457 kg) and Lidacta (8,218 kg). However, seasonal yield distribution of late maturing cultivars was better than that of early maturing cultivars. The contents of crude protein, crude fat, crude fiber, nitrogen %ee extract, aude ash, neutral detergent fiber, P, K, Ca, and Mg were not significantly different between cultivars, but higher aude protein yield was found at Potomac, Hallmark and mixture plots. Based on the above results, it may be concluded that early maturing cultivars for high productivity, late maturing cultivars for seasonal yield distribution, and seeding of mixture andlor separation of early- and late maturing cultivars are recommended. Also Lidacta was a not promising cultivar due to low productivity, severe disease, and high weeds development.

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A study on the characteristics of the conflicts occurred in the rural area development business promotions - Focused on the awareness survey of the subjects participate in regional comprehensive development project - (농촌지역개발사업 추진과정의 갈등특성 연구 - 권역단위종합정비사업 참여 주체 인식조사를 중심으로 -)

  • Shin, Yong-Gyu;Cho, Joong-Hyun
    • Journal of Korean Society of Rural Planning
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    • v.24 no.3
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    • pp.1-12
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    • 2018
  • This study aims to offer base line data for efficient conflicts control measures by first, analyzing the current situation of the regional comprehensive development project and second, identifying level, type and cause of the conflicts appeared during the development business promotion. For this purpose, theoretical side of this study establishes a basic frame by reviewing the existing researches in order to analyze the characteristics of the conflicts between the participation subjects in the development business, and the empirical side conducts survey and analysis on the awareness on these conflicts. As a result of the analysis, the survey revealed the followings. First, the respondents were aware of the overall promotions of the business quite positively, second, the conflicts were considered as a major obstacle against the development project promotion just as the other negative factors, third, majority of the responses on the seriousness of the conflicts were neutral which meant that the conflicts could be deepened by situations, fourth, most respondents had positive recognition on the needs of conflict control training and its effectiveness, and last but not least, most respondents of the survey revealed their intentions to take parts in trouble shooting from the conflicts. Based on the results above, we were able to extract several elements to consider preparing the countermeasures for the conflicts. First, specific plan should be arranged and institutionalized in order to control the conflicts between the residents at the initial stage of the development business or even before. Second, the specific plan must lower its threshold for easy participation of the residents in that region and make the residents main body of the conflict control. Third, enhanced conflict control education must be provided to the residents of the region where comprehensive development plan is in progress or being prepared.

Studies on Constituents of Higher Fungi of Korea(L VII) Comparison of Various Antitumor Constituents of Coriolus versicolor (한국산(韓國産) 고등(高等) 균류(菌類)의 성분(成分) 연구(硏究) (제 57보(第 57報)) 구름버섯 항암성분의 비교)

  • Cho, Hee-Jeong;Shim, Mi-Ja;Choi, Eung-Chil;Kim, Byong-Kak
    • The Korean Journal of Mycology
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    • v.16 no.3
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    • pp.162-174
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    • 1988
  • To separate and quantitate antitumor protein-bound polysaccharides of Coriolus versicolor, the constituents were obtained from the submerged culture of the mycelia(C) and from the extract of the carpophores of the wild fungus(N). The polysaccharides were degraded by methanolysis. The neutral monosaccharides were separated and quantitated by HPLC using microbondapak carbohydrate analysis column, refractive index detection and water-acetonitrile acetic acid elution. The analyses of these constituents by HPLC showed that the natural constituent(N) consisted of glucose, xylose and mannose, the average amount being 96.44, 2.16 and 1.73%, respectively. The fermentation constituent(C) consisted of mannose, glucose, xylose and galactose, the average amount being 61.30, 14.00, 12.95, and 11.75%, respectively. The analyses of these constituents by an amino acid analyzer showed that both C and N contained 17 amino acids.

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Enhanced Prodiginines Production in Streptomyces coelicolor M511 by Stress of Acidic pH Shock (산성 pH 충격 스트레스에 의한 스트랩토마이세스 시에리컬러 M511의 프로디지닌 생산 증대)

  • Mo, Sang-Joon
    • Microbiology and Biotechnology Letters
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    • v.38 no.3
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    • pp.273-277
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    • 2010
  • Undecylprodiginine and streptorubin B are red-pigmented antibiotics produced by Streptomyces coelicolor A3(2). In this study, we investigated the correlation between productivity of these red-pigmented antibiotics and stress of pH shock. Biosynthesis of these red-pigmented antibiotics is enhanced at acidic pH shock on solid R2YE medium. The optimal pH shock is pH 4 which led to 1.6 fold and two-fold increase in the production of undecylprodiginine and streptorubin B as compared with control, respectively. In addition, the extract of pH 4 shocked cells exhibited a remarkable activity against Trichophyton mentagrophytes. However, neutral and basic pH shock did not give raise to promote a production of these red-pigmented antibiotics as well as antifungal activity. Thus, although the acidic pH shock is simple and easy method, it should be extremely effective approach to enhance a productivity of these red-pigmented antibiotics and other secondary metabolites.

Antithrombin Active Polysaccharide Isolated from the Alkaline Extract of Red Ginseng

  • Kim Dong Chung;In Man-Jin;Lee Ji Young;Hwang Yoon Kyung;Lee Sung Dong
    • Journal of Ginseng Research
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    • v.23 no.4
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    • pp.217-221
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    • 1999
  • We have isolated an antithrombin active polysaccharide in red ginseng by procedures comprising three major steps involving alkaline extraction, anion exchange and gel permeation chromatography. Active polysaccharide behaved as a single band on cellulose acetate membrane electrophoresis. The average molecular mass was estimated to be about 177 kDa by gel filtration. This polysaccharide was found to be an acidic heteropolysaccharide that contains uronic acid moiety $(40.2\%)$, sulfate group $(9.2\%)$ and protein $(1.5\%)$ in addition to neutral sugar consisted of rhamnose, mannose, galactose, arabinose, glucose, fucose and xylose in a molar ratio of 1.00 : 0.88 : 0.86 : 0.78: 0.70 : 0.33 : 0.22. This polysaccharide inhibited blood coagulation via the intrinsic pathway like heparin in a dose-dependent manner. The clotting of fibrinogen by thrombin was also mitigated by the presence of this polysaccharide.

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Color Stability of Chlorophyll in Young Barley Leaf (새싹보리 클로로필의 색 안정화)

  • No, Junhee;Yoon, Huina;Park, Sara;Yoo, Seung Jin;Shin, Malshick
    • Journal of the East Asian Society of Dietary Life
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    • v.26 no.4
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    • pp.314-324
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    • 2016
  • To improve the color stability of chlorophyll in young barley leaf used as functional green biomaterial, the absorption spectrum, color values, and antioxidative activities of young barley leaf (YBL) treated with zinc ion solutions were investigated. The small pieces of fresh YBL in aqueous solution mixtures were autoclaved twice at $110^{\circ}C$ for 30 min (pH 5). Distilled water (BLA), 0.01% zinc chloride (BLAZ), 0.01% zinc citrate (BLAC), and 0.01% zinc lactate (BLAL) solutions were used. Treated YBL powders were extracted with 80% EtOH for 4 h. Chlorophyll a and b contents differed with different treatments. BLA decreased chlorophyll a and b contents, whereas others were maintained. Absorbance spectrums of chlorophyll at 400~700 nm showed different maximum peak wavelengths. After heating in acidic and neutral solutions (pH 3, 5, and 7), the colors of YBL and BLA changed from green to olive green, whereas BLAZ, BLAC, and BLAL remained green color. The antioxidative activities showed higher values in YBL extract than in treated extracts. From the above results, autoclaved YBL in zinc solution would increase the color stability and maintain green color regardless of acid and heat treatments.

The extraction and application of the color chip represented in the work of Picasso according to his muses (피카소의 작품에 나타난 뮤즈별 컬러 칩 도출과 적용)

  • Lee, Keum Hee
    • The Research Journal of the Costume Culture
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    • v.23 no.2
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    • pp.193-212
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    • 2015
  • The purpose of this study is to analyze the characters and images of Picasso's muses, to extract the color chip from the works of Picasso, and to apply these results to fashion design. The study includes a literature review, an analysis of visual materials. The results are as follows. Fernande, who was cheerful and voluptuous, was depicted with pink-brown, a few browns, grey, black, white, and green and represents an ethnic and elegant image. Eva, a star-crossed lover, was depicted with a few browns, black, and pale-yellow and represents a gorgeous and natural image. Olga, who was jealous and sickly wife, was depicted with a few blues, browns and neutral-color and represents a classic and dandy image. Th${\acute{e}}$r${\grave{e}}$se, who was innocent and sweet, was depicted with the bright and pastel colors of red, green, violet, and yellow and of white, blue, brown, and black and represents a romantic, pretty, and casual image. Dora, who was intellectually and emotionally- challenging, was depicted with the strong colors of red, yellow, green, and blue, which are in contrast to black and also to white, grey and brown and represents a wild and dynamic image. Gilot, who was strong-willed and prideful, was depicted with the symbolic color of green, a color contrasting with black and white, grey, and pale brown and represents a clear, cool casual and modern image. Jacqueline, the self-giving and reclusive wife, was depicted with deep red, blue, green, black, white, dark brown, grey represents a formal, dandy, and chic image.

Classification of ratings in online reviews (온라인 리뷰에서 평점의 분류)

  • Choi, Dongjun;Choi, Hosik;Park, Changyi
    • Journal of the Korean Data and Information Science Society
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    • v.27 no.4
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    • pp.845-854
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    • 2016
  • Sentiment analysis or opinion mining is a technique of text mining employed to identify subjective information or opinions of an individual from documents in blogs, reviews, articles, or social networks. In the literature, only a problem of binary classification of ratings based on review texts in an online review. However, because there can be positive or negative reviews as well as neutral reviews, a multi-class classification will be more appropriate than the binary classification. To this end, we consider the multi-class classification of ratings based on review texts. In the preprocessing stage, we extract words related with ratings using chi-square statistic. Then the extracted words are used as input variables to multi-class classifiers such as support vector machines and proportional odds model to compare their predictive performances.

Phenolic Compounds from the Flower Buds of Camellia japonica

  • Cho, Jeong-Yong;Ryu, Hyun-Jung;Ji, Soo-Hyun;Moon, Jae-Hak;Jung, Kyung-Hee;Park, Keun-Hyung
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.766-770
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    • 2009
  • Hot water extracts of Camellia japonica flower buds were found to have the higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the other solvent extracts. Five phenolic compounds were isolated and purified from the ethyl acetate soluble-neutral fraction of hot water extracts by Sephadex LH-20 column chromatography and octadecyl silane-high performance liquid chromatography using the guided assay of DPPH radical scavenging. Based on mass spectrometer and nuclear magnetic resonance, the isolated compounds were identified as p-hydroxybenzaldehyde (1), vanillin (2), dehydroxysynapyl alcohol (3), 7S,7'S,8R,8'R-icariol $A_2$ (4), and (-)-epicatechin (5). Four compounds (1-4) except for 5 were newly identified in this plant. Their DPPH radical scavenging activities as 50% scavenging concentration decreased in order to 5 $(20\;{\mu}M)>{\alpha}-tocopherol$ $(29\;{\mu}M)>4$ $(67\;{\mu}M)>3$ $(72\;{\mu}M)>1=2$ ($>250\;{\mu}M$). These results indicate that the antioxidant effect of the hot water extract of C. japonica flower buds may partially due to 5 isolated phenolic compounds.

Purification and Characterization of stu I Endomuclease from Streptomyces Tubercidicus (Streptomyces tubercidicus에 존재하는 stu I endonuclease의 정제와 특징)

  • 김기태;정미영;유욱준
    • Korean Journal of Microbiology
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    • v.25 no.3
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    • pp.180-183
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    • 1987
  • Stu I, type II restriction endonuclease, has been purified to homogeneity from Streptomyces tubercidicus (ATCC 25502), and its catalytic properties have been studied. For the purification of Stu I endonuclease free of nonspecific nucleases, DEAE-Sephadex (A-50), QAE-Sephadex (A-50) and Heparin-agarose column chromatography have been performed after ammonium sulfate fractionation of the crude extract. The enzyme was further purified by gel filtration using Sephadex G-100 column to obtain homogeneous form of protein. The single polypeptide species of Stu I endonuclease has a subunit molecular weight of 34,000 $\pm$ 1,000 daltons as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Stu I endonuclease requires $Mg^{2+}$ ion for its activity and is maximally active at neutral pH (7.0-8.0) in the absence of NaCl.

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