• Korean journal of applied entomology
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• v.34 no.3
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• pp.224-228
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• 1995

During the survey on spiral nematode (Hoplolaimidae) from Korea, Pararotylenchus blothrotylus Baldwin & Bel, 1981, Rotylenchus feroxcis Eroshenko, 1981 and Scutellonema conicephalum Sivakumar, 1982 were newly recorded from Korea.

• The Plant Pathology Journal
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• v.23 no.3
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• pp.210-211
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• 2007

Dactylella pseudoclavata that captures nematodes in adhesive networks was isolated from nematode-infested strawberry plants from Andong, Korea. It produces obclavate, 0-1 septate conidia, $30-40{\mu}m$ long and $8-11{\mu}m$ wide, with round distal ends and bases shaped like bottle-necks. The conidiophores were simple, occasionally branched, $150-300{\mu}m$ long, producing 1-4 conidia at the apex. Chlamydospores were abundant, intercalary or catenulate, yellowish to brown, globate or subglobate, wart on the surface, $30-35{\times}25-30{\mu}m$ in size. This is the first report of Dactylella pseudoclavata in Korea.

• Parasites, Hosts and Diseases
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• v.53 no.6
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• pp.713-717
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• 2015

A 23-year-old female residing in a village of Cao Bang Province, North Vietnam, visited the Hospital of Hanoi Medical University in July 2013. She felt dim eyes and a bulge-sticking pain in her left eye for some days before visiting the hospital. In the hospital, a clinical examination, an eye endoscopy, and an operation were carried out. A nematode specimen was collected from the eye of this patient. The body of this worm was thin and long and measured $22.0{\times}0.3mm$. It was morphologically suggested as an immature female worm of Angiostrongylus cantonensis. By a molecular method using 18S rRNA gene, this nematode was confirmed as A. cantonensis. This is the first molecular study for identification of A. cantonensis in Vietnam.

• Journal of Korean Society of Forest Science
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• v.112 no.2
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• pp.258-266
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• 2023

Pine wilt disease is caused by the pine wood nematode (Bursaphelenchus xylophilus) and has killed many pine trees in Europe and Northeast Asia, including South Korea. Resistance to pine wilt disease varies among species. Previous studies were mostly conducted in nature or greenhouses and only a few in test fields. In this study, seedlings of six pine species (Pinus thunbergii, P. koraiensis, P. densiflora, P. parviflora, P. rigida × P. taeda, and P. strobus) were artificially inoculated by pine wood nematodes in the test field. The Wilt Index was measured every 2 weeks after inoculation in addition to the mortality rate, detection rate, and pine wood nematode concentration measurement after 24 weeks. The pine wilt disease mortality rates were P. thunbergii (80%), P. koraiensis (77.8%), P. densiflora (62.5%), and P. parviflora (22.0%), and both P. rigida × P. taeda and P. strobus survived. The pine nematode detection rates were the same among the species except for P. rigida × P. taeda pine (22.2%). High Wilt-Index values were obtained for P. thunbergii, P. koraiensis, and P. densiflora, which had mortality rates higher than the other species. Furthermore, there were no significant differences in the Wilt Indexes between P. parviflora, P. rigida × P. taeda, P. strobus, and the control group. Statistically, P. thunbergii and P. koraiensis showed high susceptibility to pine wilt disease, P. densiflora and P. parviflora showed moderate susceptibility, and P. rigida × P. taeda and P. strobus showed apparent resistance. These results provide basic data for pine wood nematode resistance breeding or as evidence of the need for afforestation of P. rigida × P. taeda and P. strobus.

• Korean Journal of Veterinary Research
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• v.30 no.4
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• pp.499-505
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• 1990

The seasonal availability and abundance of the free-living stages of sheep nematodes is a key factor in the occurrence and severity of parasitic infection, and studies of larvae ecology could result in more rational control measures. In the present study seasonal pasture contamination and availability of nematodes for grazing sheep was examined as a baseline work for nematode control program at Namwon Branch, National Animal Breeding Institute during the period April 1988 through March 1989. Standard meteorological measurements were available from Unbong Sub-station, Honam Crops Experiment Station located about 200m from the experimental site. A total of 5 kinds of nematode larvae was detected: Haemonchus contortus was most prevalent (38.0%) with a decreasing order of Ostertagia spp (35.1%), Trichostrongylus spp (19.8%), nematodirus spp (6.6%), and Oesophagostomum spp (0.5%). A succession of species was recorded, in particular Ostertagia spp in May; Haemonchus contortus in June and July; Trichostrongylus spp in July and August; Nematodirus spp in August and September. These results can be incorporated into the nematode control program. To make a more rational control program, however, repeated herbage larval counts should be undertaken soil larval counts and fecal larval counts in the future.

• Journal of Environmental Science International
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• v.28 no.1
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• pp.75-84
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• 2019

Cytochrome c oxidase subunit II gene(CO II gene) is subunit of cytochrome oxidase, which is complex IV of mitochondria electron transport system. It has been frequently used in molecular phylogenetic studies because the speed of its DNA variation is faster than that of nucleus. It is especially useful in phylogenetic study of molecular biology in insects. In this study, we cloned and sequenced CO II gene of mitochondria DNA from Rhabditidae family nematode. Our results showed that this gene is comprised of 696 base pairs(bp). In the analysis of similarity of this gene with other known genes of 14 species of nematodes in Rhabditida order, we identified that this gene has high similarity with that of Caenorhabditis briggsae(86.0%) and C. elegans(85.6%) in Rhabditidae family. On the meanwhile, it has very low similarity with that of Angiostrongylus cantonensis(31.8%) in Angiostrongylidae family and Metastrongylus salmi(31.6%) in Metastrongylidae family. Based on the results of this study, we suggest that this nematode is closely related with that of Caenorhabditis genus in Rhabditidae family.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.217-226
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• 2014

Root-knot symptoms were found on a commercial tomato cultivar carrying Mi, a resistance gene to root-knot nematodes including Meloidogyne incognita, M. arenaria, and M. javanica in 2012 at Buyeo, Chungnam Province in Korea. The isolate was identified as M. incognita based on molecular analyses using two species-specific primer sets. Pathogenicity of the isolate on one susceptible and three resistant tomato cultivars to the root-knot nematodes was tested. The nematode isolate showed strong pathogenicity on all the tested cultivars at all tested incubation temperatures. In addition, resistance degree of 33 commercial tomato cultivars, 8 susceptible and 25 resistant cultivars to root-knot nematodes, was also tested. Plants were determined as resistant when they suppressed the nematode reproduction. All the cultivars demonstrated strong susceptibility to the nematode regardless of resistance of the tomato cultivars. To our knowledge, this is the first report on the occurrence of Mi infecting M. incognita isolate in Korea. On the other hand, to construct an efficient screening method for selecting resistant breeding source to the nematode isolate, root-knot development of M. incognita on four tomato cultivars according to several conditions such as inoculum concentration, plant growth stage, and incubation period after transplant was investigated. Reproduction of the nematode on all the tested cultivars according to inoculum concentration increased in a dose-dependent manner. Except for inoculum concentration, there was no significant difference in reproduction level of the cultivars according to the other tested conditions. On the basis of the results, we suggest an efficient screening method for new resistant tomato to the nematode isolate.

• Parasites, Hosts and Diseases
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• v.53 no.1
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• pp.135-139
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• 2015

A total of 1,708 small mammals (1,617 rodents and 91 soricomorphs), including Apodemus agrarius (n = 1,400), Microtus fortis (167), Crocidura lasiura (91), Mus musculus (32), Myodes (= Eothenomys) regulus (9), Micromys minutus (6), and Tscherskia (= Cricetulus) triton (3), were live-trapped at US/Republic of Korea (ROK) military training sites near the demilitarized zone (DMZ) of Paju, Pocheon, and Yeoncheon, Gyeonggi Province from December 2004 to December 2009. Small mammals were examined for their intestinal nematodes by necropsy. A total of 1,617 rodents (100%) and 91 (100%) soricomorphs were infected with at least 1 nematode species, including Nippostrongylus brasiliensis, Heligmosomoides polygyrus, Syphacia obvelata, Heterakis spumosa, Protospirura muris, Capillaria spp., Trichuris muris, Rictularia affinis, and an unidentified species. N. brasiliensis was the most common species infecting small mammals (1,060; 62.1%) followed by H. polygyrus (617; 36.1%), S. obvelata (370; 21.7%), H. spumosa (314; 18.4%), P. muris (123; 7.2%), and Capillaria spp. (59; 3.5%). Low infection rates (0.1-0.8%) were observed for T. muris, R. affinis, and an unidentified species. The number of recovered worms was highest for N. brasiliensis (21,623 worms; mean 20.4 worms/infected specimen) followed by S. obvelata (9,235; 25.0 worms), H. polygyrus (4,122; 6.7 worms), and H. spumosa (1,160; 3.7 worms). A. agrarius demonstrated the highest prevalence for N. brasiliensis (70.9%), followed by M. minutus (50.0%), T. triton (33.3%), M. fortis (28.1%), M. musculus (15.6%), C. lasiura (13.2%), and M. regulus (0%). This is the first report of nematode infections in small mammals captured near the DMZ in ROK.

• Animal Systematics, Evolution and Diversity
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• v.34 no.4
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• pp.194-198
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• 2018

The genus Prionchulus Cobb, 1916 represents a group of predaceous nematodes belonging to the family Mononchidae Chitwood, 1937, and is found worldwide. However, only five species have been reported thus far from Korea. Prionchulus oleksandri Winiszewska and Susulovsky, 2003 is reported for the first time from Korea, from sediments collected from the Nakdong River. This species is distinguished from other Prionchulus species by its truncated lip region with small cephalic papillae and refringens vaginae. In this study, morphological characters(detailed morphometrics) of P. oleksandri are described and illustrated using optical microscopy. DNA barcode sequence information (the D2-D3 region of 28S rDNA, 18S rDNA, and internal transcribed spacer rDNA) is also provided for the molecular identification of the species.

• Journal of Species Research
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• v.10 no.4
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• pp.405-411
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• 2021

The genus Acrobeloides(Cobb, 1924) Thorne, 1937 are bacterial feeders and are one of the most abundant and widely distributed nematode groups in various terrestrial environments. Based on morphological and morphometric analyses, we found two Acrobeloides species reported in Korea for the first time: A. bodenheimeri (Steiner, 1936) Thorne, 1937 and A. tricornis (Throne, 1925) Thorne, 1937. These species exhibit morphological characters concordant with typical features of the genus Acrobeloides, such as a fusiform pharyngeal corpus with swollen metacorpus and lateral incisures extending to the tail terminus. However, A. bodenheimeri is distinguished from other acrobeloids by having its low and rounded labial probolae, distinct post-uterine sac and five lateral incisures. Acrobeloides tricornis is distinguished from its congeners by the following characteristics: its high labial probolae with acuate termini, inconspicuous post-uterine sac and five lateral incisures. Morphological characters and their measurements, and illustrations of A. bodenheimeri and A. tricornis are described in this study.