• 제목/요약/키워드: neem

검색결과 92건 처리시간 0.033초

Variability of Azadirachtin in Azadirachta indica (neem) and Batch Kinetics Studies of Cell Suspension Culture

  • Prakash Gunjan;Emmannuel C.J.S.K.;Srivastava Ashok K.
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제10권3호
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    • pp.198-204
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    • 2005
  • Seeds of neem were collected from different parts of India and analyzed for their azadirachtin content by High Performance Liquid Chromatography (HPLC). In order to assess the effects of genotypic and geographical variation on azadirachtin content in cell cultures, callus development was attempted in the seeds containing high and low concentration of azadirachtin. The concentration of azadirachtin in callus cultures was significantly affected by the explant source. Seed kernels with higher azadirachtin content produced higher azadirachtin content in callus cultures and lower azadirachtin content was seen in callus cultures produced from seed kernels with low azadirachtin content. The protocol for development of elite stock culture of Azadirachta indica was established with the objective of selecting a high azadirachtin-producing cell line. The highest azadirachtin-producing cell line was selected and the effects of different media and illumination conditions on growth and azadirachtin production were studied in shake flask suspension culture. Detailed batch growth kinetics was also established. These studies provided elite starter culture and associated protocols for cultivation of A. indica plant cell culture in the bioreactor.

Simple Self Trap Cropping System to Control Tukra Mealy Bug (Maconellicoccus hirsutus Green) Incidence on Mulberry (Morus spp.)

  • Latha, K. Lavanya;Rao, T.V.S.S.;Rao, J. V. Krishna;Jayaraj, S.;Reddy, N. Sivarami;Naik, S. Sankar
    • International Journal of Industrial Entomology and Biomaterials
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    • 제17권2호
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    • pp.201-203
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    • 2008
  • A field study on self trap cropping system in controlling tukra mealy bug Maconellicoccus hirsutus Green) incidence on mulberry (Morus spp.; V-1 variety; $3'{\times}3'$ spacing) gardens was undertaken in selected sericulture farmers' mulberry gardens of Pydeti village (Parigi Mandal, Anantapur District, Andhra Pradesh, India). The study was conducted for three years (2004, 2005 and 2006). Four treatments (T0; control/no treatment, T1; self trap cropping rows with 10 row interval, T2; self trap cropping rows with 10 row interval and treating these rows only with 0.5% neem oil emulsion and T3; self trap cropping rows with 10 rows intervals and treating all the mulberry rows including self trap cropping rows with 0.5% neem oil emulsion) were considered. The results indicated that the incidence increased in T0 and T1 while the same suppressed in T2 and T3. The suppression (%) of tukra incidence between T2 and T3 was not significant. Hence, T2 only was recommended to farmers as its economical viable practice. The results are discussed based on the importance of tukra, its suppression and cost of treatment.

Nanocarbon synthesis using plant oil and differential responses to various parameters optimized using the Taguchi method

  • Tripathi, Suman;Sharon, Maheshwar;Maldar, N.N.;Shukla, Jayashri;Sharon, Madhuri
    • Carbon letters
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    • 제14권4호
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    • pp.210-217
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    • 2013
  • The synthesis of carbon nanomaterials (CNMs) by a chemical vapor deposition method using three different plant oils as precursors is presented. Because there are four parameters involved in the synthesis of CNM (i.e., the precursor, reaction temperature of the furnace, catalysts, and the carrier gas), each having three variables, it was decided to use the Taguchi optimization method with the 'the larger the better' concept. The best parameter regarding the yield of carbon varied for each type of precursor oil. It was a temperature of $900^{\circ}C$ + Ni as a catalyst for neem oil; $700^{\circ}C$ + Co for karanja oil and $500^{\circ}C$ + Zn as a catalyst for castor oil. The morphology of the nanocarbon produced was also impacted by different parameters. Neem oil and castor oil produced carbon nanotube (CNT) at $900^{\circ}C$; at lower temperatures, sphere-like structures developed. In contrast, karanja oil produced CNTs at all the assessed temperatures. X-ray diffraction and Raman diffraction analyses confirmed that the nanocarbon (both carbon nano beads and CNTs) produced were graphitic in nature.

엽채류 유기재배의 병해충 관리 (Control of Diseases and Insects for Pesticide-free Cultivation of Leafy Vegetables)

  • 서영호;조병욱;최준근;강안석;정병찬
    • 한국유기농업학회지
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    • 제17권2호
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    • pp.253-264
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    • 2009
  • 상추의 가을재배에서 공기 교반 장치로 실내 온도와 습도를 조절해 주면 잿빛곰팡이병(B. cinerea)과 무름병(E. carotovora)이 감소되었으며, 수량도 30% 이상 증수되었다. 배추의 진딧물 밀도는 천적인 무당벌레(H. axyridis)와 콜레마니진디벌(A. colemani)을 방사하며 현저히 감소되었다. 난황유와 BT제를 혼합하여 살포하면 배추의 무름병(E. carotovora), 노균병(P. brassicae), 흰가루병(E. polygoni)의 발생과 배추좀나방(P. xylostella)과 파밤나방(S. exigua)의 발생 밀도가 현저하게 감소하였다. 파의 노균병(P. destructor) 발생은 Neem 추출물을 처리하여 무처리 대비 60% 이상 낮출 수 있었다. 파좀나방의 충해는 잎굴파리고치벌에 의해 23% 감소되었다.

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Review on Molecular and Chemopreventive Potential of Nimbolide in Cancer

  • Elumalai, Perumal;Arunakaran, Jagadeesan
    • Genomics & Informatics
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    • 제12권4호
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    • pp.156-164
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    • 2014
  • Cancer is the most dreaded disease in human and also major health problem worldwide. Despite its high occurrence, the exact molecular mechanisms of the development and progression are not fully understood. The existing cancer therapy based on allopathic medicine is expensive, exhibits side effects; and may also alter the normal functioning of genes. Thus, a non-toxic and effective mode of treatment is needed to control cancer development and progression. Some medicinal plants offer a safe, effective and affordable remedy to control the cancer progression. Nimbolide, a limnoid derived from the neem (Azadirachta indica) leaves and flowers of neem, is widely used in traditional medical practices for treating various human diseases. Nimbolide exhibits several pharmacological effects among which its anticancer activity is the most promising. The previous studies carried out over the decades have shown that nimbolide inhibits cell proliferation and metastasis of cancer cells. This review highlights the current knowledge on the molecular targets that contribute to the observed anticancer activity of nimbolide related to induction of apoptosis and cell cycle arrest; and inhibition of signaling pathways related to cancer progression.

7종의 주요 해충에 대한 식물추출물의 살란 효과 (Ovicidal Effect of Plant Extract Mixture Against Seven Major Insect Pests)

  • 이희아;이영수
    • 한국응용곤충학회지
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    • 제63권1호
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    • pp.75-76
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    • 2024
  • 몇 종의 주요 농작물 해충에 대한 식물추출물(cinnamon extract 5% + citronella oil 10% + citrus oil 30% + derris extract 10% + neem extract 20% + penetrating surfactant 25%)의 살란 효과를 분무법으로 검정하였다. 노린재류의 경우 알이 부화하는 과정에서 치사하는 경향을 보였다. 식물추출물(500배액) 처리시 썩덩나무노린재(Halyomorpha halys), 톱다리개미허리노린재(Riptortus clavatus), 홍비단노린재(Eurydema dominulus), 온실가루이(Trialeurodes vaprarorium), 담배가루이(Bemisia tabaci), 파밤나방(Spodoptera exigua), 검거세미밤나방(Agrotis ipsilon)에 대한 최종 살란 효과는 100%로 높게 나타나, 향후 산업화가 가능할 것으로 생각된다.

친환경 육묘시 세 가지 박과채소의 흰가루병에 대한 친환경 제제의 방제효과 (Evaluation of Environment-friendly Control Agents for the Management of Powdery Mildew Infection during Seedling Stage of Three Cucurbitaceae Vegetables)

  • 여경환;장윤아;김수;엄영철;이상규;이한철
    • 생물환경조절학회지
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    • 제22권4호
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    • pp.413-420
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    • 2013
  • 본 실험은 박과류(오이, 참외, 멜론)를 공시작물로 하여 친환경 육묘시 친환경 제제와 시용조건에 따른 흰가루병의 방제효과를 구명하고 기존 등록된 농약과 대조하여 그 효과를 검정하기 위해 수행되었다. 친환경제제는 유효미생물로서 Ampelomyces quisqualis 94013(AQ94013)과 Bacillus subtilis Y1336(BS Y1336), 식물추출물로서 님오일(Neem oil)과 대황(Rheum undulatum)추출물, 미네랄 제제는 황수화제와 석회보르도액(Lime Bordeaux mixture)을 단용 또는 혼용처리하여 병 발생시기(발생전, 초기, 중기)에 따라 처리하였다. 모든 처리구에서 시설내 일 평균온도 $30^{\circ}C$ 이상, 하루 중 최고기온이 $40^{\circ}C$ 이상의 조건이 일주일 이상 지속되는 기간 동안 유묘의 흰가루병의 발병도는 감소하였고, 대조구인 무처리구에서의 발병도도 크게 감소되어 10% 미만으로 나타났다. 발생 전처리시 방제효과는 모든 작물에서 황수화제가 가장 높았고, BS Y1336는 방제가가 20~40%로서 가장 낮게 나타났다. 오이의 경우 발생전 처리시 님오일과 황수화제 처리는 살균제보다 높은 방제효과를 보였으며, 멜론에서는 농약과 비슷한 방제가를 보였다. 대황추출물은 전 생육기간 동안 미생물제제보다 높은 방제가를 나타내었고, 오이와 멜론작물에서 발생초기 처리시 효과가 우수하였다. 물살포구는 무처리구와 비슷한 발병도를 보여 방제효과가 없었고, 참외와 멜론에서 물살포 처리는 무처리보다 더 높은 발병도를 보였다. 병발생후 친환경제제 처리시 방제효과는 처리하는 시기가 늦어질수록 감소하였다. 친환경제제의 혼용처리에 의한 방제효과를 조사하였을 때, 대황추출물(1주차) + 황수화제(2주차) + 님오일(3주차)의 조합으로 한 가지의 친환경제제를 주당 1회씩 총 3주 동안 혼용처리 했을 때, 전 육묘기간 동안 오이에서는 90% 이상, 모든 작물에서 평균 80% 이상의 방제가를 나타내어 다른 조합의 처리에 비해 높은 방제효과를 보였다. 본 실험결과를 통해 박과채소의 친환경 육묘시 흰가루병에 대한 친환경제제의 방제효과는 단용처리 했을 때, 병발생 전후 처리에 상관없이 모든 작물에서 황수화제 처리가 가장 높게 나타났으며, 혼용처리시에는 대황추출물(1주차) + 황수화제(2주차) + 님오일(3주차)의 조합이 가장 효과가 높은 것으로 나타났다.