• 대한한방내과학회지
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• 제29권1호
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• pp.160-176
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• 2008

Objective : We designed this study to analyse the Natural Standard methodology and apply the results to new study and evaluation methods. Methods : We investigated the main stream of complementary and alternative medicine (CAM), the methodology of Natural Standard, guideline in CAM, and concrete 35 cancer-related herbs and dietary supplements in 98 ones. Results : There are 35 graded herbs and supplements associated with cancer in natural standard contents: 9 foods, 17 herbs, 7 amino acids, enzymes and vitamins, and 2 herbal mixture formulas. Most of them get a grade of C: unclear or conflicting scientific evidence. Conclusion : Natural Standard aims to provide high-quality, reliable information about CAM therapies to clinicians, patients, and healthcare institutions. The more demands for and use of CAM grows, the more the needs for related studies increases. According to this international and multidisciplinary collaborative effort, we have to develop how to study and evaluate the results. We need guidelines in association with the evidence and recommendations of CAM.

• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.353-361
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• 2012

Human embryonic stem (ES) cells are a potential source of cells for developmental studies and for a variety of applications in transplantation therapies and drug discovery. However, human ES cells are difficult to culture and maintain at a large scale, which is one of the most serious obstacles in human ES cell research. Culture of human ES cells on MEF cells after disassociation with accutase has previously been demonstrated by other research groups. Here, we confirmed that human ES cells (H9) can maintain stem cell properties when the cells are passaged as single cells under a feeder-free culture condition. Accutase-dissociated human ES cells showed normal karyotype, stem cell marker expression, and morphology. We prepared frozen stocks during the culture period, thawed two of the human ES cell stocks, and analyzed the cells after culture with the same method. Although the cells revealed normal expression of stem cell marker genes, they had abnormal karyotypes. Therefore, we suggest that accutase-dissociated single cells can be usefully expanded in a feeder-free condition but chromosomal modification should be considered in the culture after freeze-thawing.

• 대한한의학회지
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• 제35권2호
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• pp.34-40
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• 2014

Objective: This study aimed to investigate the demand for natural environment therapy among parents of patients with atopic dermatitis. Methods: The questionnaire-based survey was conducted on the parents of 310 patients with atopic dermatitis (158 male and 152 female, median age 8 years, ranging from 2 to 21 years). The questionnaire consisted of 10 questions including demographic information, preference of environmental therapy and related choices. Results: Regardless of illness severity or gender, parents agreed with the importance of environmental therapy in treatment for atopic dermatitis. 55.2% of parents had considered moving to the countryside for their children with atopic dermatitis. 74.5% of parents answered positively to the possibility of movement to an atopy-free village, and this rate was significantly higher in parents of patients with severe symptoms (p < 0.05). Educational facilities and eco-environment were considered to be the most important factors in their decision. In addition, parents opted for organic food cooperatives, which was also felt necessary for their children suffering from atopic dermatitis. Conclusions: This study is the first report suggesting the extent of demand for environmental therapies among patients with atopic dermatitis. This information will be helpful in developing therapeutics using the natural environment in Korean medicine.

• Asian-Australasian Journal of Animal Sciences
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• 제33권11호
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• pp.1699-1713
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• 2020

Bovine mastitis, an inflammation of the mammary gland, is the most common disease of dairy cattle causing economic losses due to reduced yield and poor quality of milk. The etiological agents include a variety of gram-positive and gram-negative bacteria, and can be either contagious (e.g., Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma spp.) or environmental (e.g., Escherichia coli, Enterococcus spp., coagulase-negative Staphylococcus, Streptococcus uberis). Improving sanitation such as enhanced milking hygiene, implementation of post-milking teat disinfection, maintenance of milking machines are general measures to prevent new cases of mastitis, but treatment of active mastitis infection is dependant mainly on antibiotics. However, the extensive use of antibiotics increased concerns about emergence of antibiotic-resistant pathogens and that led the dairy industries to reduce the use of antibiotics. Therefore, alternative therapies for prevention and treatment of bovine mastitis, particularly natural products from plants and animals, have been sought. This review provides an overview of bovine mastitis in the aspects of risk factors, control and treatments, and emerging therapeutic alternatives in the control of bovine mastitis.

• Bulletin of the Korean Chemical Society
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• 제28권1호
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• pp.49-52
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• 2007

Prodigiosin (1) is the parent member of a class of polypyrrole natural products that exhibit promising anticancer activities. They can facilitate copper-promoted oxidative DNA damage by binding to copper ions, and this activity is thought to represent their mechanism of cytotoxicity in the dark. They also possess photoinduced cytotoxicity, although 1 is too toxic in the dark to be used effectively for the treatment of cancer by photodynamic therapies. To circumvent dark toxicity by prodigiosins, the semi-synthetic analogue 2, in which the N-pyrrolic atoms of 1 are methylated to block copper coordination, and the synthetic phenyl analogues 3 and 4, which lack the copper-coordinating A-pyrrole ring of 1, were tested for their ability to inhibit colony formation of HL-60 cancer cells in the absence and presence of visible light (λ > 495 nm). Our results show that 2-4 lack cytotoxicity in the dark, but are able to inhibit colony formation of HL-60 cells following irradiation for 30 min. The synthetic derivative 4 exhibits photo-induced cytotoxicity similar to that of the natural product 1, demonstrating the potential use of prodigiosin-based compounds for treatment of cancers following irradiation with visible light.

• 대한한의학방제학회지
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• 제21권2호
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• pp.100-109
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• 2013

Objectives : "Donguibogam(東醫寶鑑)" is a medical book of the Joseon Dynasty compiled by Heo Jun and was first published in 1613. It recorded a great deal of prescriptions which contained pork and gave detailed explanations of parts of pork's natural properties and effects. Pork is widely used as a food ingredient in the world but the traditional medicine community has a contraindication of pork and traditional herbal medicines taken together. The aim of this study was to find the supports to develop pork functional foods for the treatment of diseases in "Donguibogam". Methods : Therefore we investigated the documents recorded in "Donguibogam" about usefulness of pork which were used for not only a food ingredient but also a medicine. Results : "Donguibogam" introduced 25 parts of pork including liver, kidney, stomach, bladder, heart etc, and a variety of taking ways i.e. common usages which are internal, external, common use, and aid use which cure a variety of diseases not a simple food but a therapies food. Conclusions : We confirmed that pork has been used to treat many diseases, so we propose to develop pork functional foods available for prevention and cure of certain diseases.

• 대한한방종양학회지
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• 제6권1호
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• pp.125-148
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• 2000

At here, we investigated the historical background, current stage, and concrete therapies of alternatives in cancer therapy. We obtained the conclusion below. 1. Alternative medicine(AM) is a unothodoxial medical physiology, medical practice or intervension that overcome the side effects, faults, limits of coventional medicine. 2. Recentely, as emerging New-age movement, collapse of Decarte's mecanical cosmology, and emphasis on patient rights, the interest of AM is increased, and foreign & domestic use rates of AM are risen. 3. After coventional medicine in cancer therapies that depends on operations, radiations, and chemotherapy is bounded, Alternatives in cancer therapy are rapidly developed, and the majority of users are often considered satisfactory. 4. Alternatives in cancer therapy are classified six boundaries; Diet and Nutrition, Mind-body Technique, Integrated System, Pharmacologic and Biologic treatments, Immuno Enhanced Therapy, Natural and herbal medicine. 5. Looking at contents of Alternatives in cancer therapy, we get to know most of them are similar to the treatment of oriental medicine in holistic method, therapy, philosophy. If we synthesize the alternative medicine's treatment mentioned above and investigate it with oriental medical viewpoint, that may help us approach the conquest of cancer and improve the rate of cure. 6. It is certain that foreign AM will be imported reversely, and we must prepare for rebuilding of Korean traditional AM systemically, investing for activity positively, making national policy for medical system.

• International Journal of Oral Biology
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• 제42권4호
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• pp.163-168
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• 2017

Osteoporosis is a metabolic bone disease that is characterized by low bone mass resulting from an increase in bone resorption relative to bone formation. The most current therapies for osteoporosis have focused on inhibiting bone resorption by osteoclasts. The purpose of this study is to develop new anabolic agents for treatment of osteoporosis that have fewer risks compared to conventional therapies. We searched the natural products that were derived from the traditional Asian medicines which have been used for treatment of bone related diseases. Icaritin is a flavonoid glycoside derived from the herb Epimedium which has beneficial effects on bone formation. To determine the effect of icaritin on bone formation, we examined the effect of icaritin on MC3T3-E1 cell proliferation and differentiation. For determining the effects of icaritin on proliferation, we performed the MTT assay using MC3T3-E1 cells. To evaluate whether icaritin could promote the osteogenic differentiation of MC3T3-E1 cells, alkaline phosphatase (ALP) activity and mRNA expressions of Runx2, osteocalcin (OCN), RANKL, and osteoprotegerin (OPG) were determined. Icaritin increased MC3T3-E1 cell proliferation. Icaritin increased the ALP activity of MC3T3-E1 cells on 72 hour culture in osteogenic media. mRNA expression of Runx2 was increased after 24 hour culture with icaritin. mRNA expression of osteocalcin was increased after 72 hour culture with icaritin. In addition, icaritin increased the mRNA expressions of OPG and RANKL. However, icaritin increased the mRNA expression of OPG much more than that of RANKL, and then, it increased the OPG/RANKL ratio. These results suggest that icaritin promotes osteogenic differentiation of osteoblasts and decreases osteoclast formation regulated by osteoblasts.

• Biomolecules & Therapeutics
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• 제32권3호
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• pp.267-280
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• 2024

Apoptosis, programmed cell death pathway, is a vital physiological mechanism that ensures cellular homeostasis and overall cellular well-being. In the context of cancer, where evasion of apoptosis is a hallmark, the overexpression of anti-apoptotic proteins like Bcl2, Bcl-xL and Mcl-1 has been documented. Consequently, these proteins have emerged as promising targets for therapeutic interventions. The BCL-2 protein family is central to apoptosis and plays a significant importance in determining cellular fate serving as a critical determinant in this biological process. This review offers a comprehensive exploration of the BCL-2 protein family, emphasizing its dual nature. Specifically, certain members of this family promote cell survival (known as anti-apoptotic proteins), while others are involved in facilitating cell death (referred to as pro-apoptotic and BH3-only proteins). The potential of directly targeting these proteins is examined, particularly due to their involvement in conferring resistance to traditional cancer therapies. The effectiveness of such targeting strategies is also discussed, considering the tumor's propensity for anti-apoptotic pathways. Furthermore, the review highlights emerging research on combination therapies, where BCL-2 inhibitors are used synergistically with other treatments to enhance therapeutic outcomes. By understanding and manipulating the BCL-2 family and its associated pathways, we open doors to innovative and more effective cancer treatments, offering hope for resistant and aggressive cases.

• 대한본초학회지
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• 제32권6호
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• pp.31-39
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• 2017

Objectives : Menopausal symptoms are characteristic symptoms which are occur in women before and after menopause. In Western medicine, hormone therapies are mainly used but patients show reluctance because they exhibit serious side effects. In Oriental medicine, there are also limits to the treatment. So I tried to find a new easily accessible treatment. I performed the experiments to verify the effect of natural dyed apparel with Indigo and Schisandra fruit. Methods : This experiment was performed on 30 women who were experiencing menopausal symptoms. We checked the participant's general condition through questionnaire and physical condition with inbody test and made natural-dyed living suit style dresses. We asked the women to wear the apparel for four weeks. They checked for changes in symptoms weekly with kupperman's index. Results : The main symptoms of participants are sweating, hot flushes, joint pain. After wearing of the apparel, there were decreased hot flushes, sleep improvement, psychological stability etc. According to the kupperman's index, patients with mild symptoms were 13.3% at first week to start the experiment but four weeks later, patients with mild symptoms were increased to 50%. On the other hand, patients with severe symptoms were decreased 33.3% to 6.7%. In the test results, there was a significant decrease in 1, 2 and 3 weeks, there was a decrease in 4 weeks but no significance in the figure Conclusion : Natural dyed apprel with Indigo and Schisandra fruit was effective on treating women who were experiencing menopausal symptoms.