• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1993년도 천연항암자원의 개발에 관한 국제학술회의
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• pp.14-20
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• 1993

We in anticancer drug development from natural resources have conceived and used a wide variety of experimental screening systems to support our efforts during the past 20 tears. Screens have been devided to address targets at the molecular, biochemical and cellular levels, both in vivo and in vitro. Screens have been essential for the experimental evaluation of the products from natural sources. In this congress, antitumor screening methods for deveol[ment of new drugs from natural sources and evaluation of their crude drugs are discussed.

• 약학회지
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• 제45권3호
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• pp.245-250
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• 2001

Aminoacyl tRNA synthetases of bacteria are known as potential targets for new anti-microbial agents. To isolate new inhibitors of bacterial methionyl-tRNA synthetases from natural sources, a new target-oriented screening system using whole cells which are over-expressing a target enzyme was developed. Approximately 8,000 culture broths of microorganisms from soils were tested by this screening system. Among them, ten culture broths was found to contain inhibitory activity against methionyl -tRNA synthetases of Escherichia coli. For the validation of the screening system, this new method was compared with in vitro enzymatic method. Seven out of 10 culture broths showed inhibitory activity against methionyl-tRNA synthetases of E. coli. This result showed that the new screening system was comparable to the enzyme assay. Thus we believe that our screening system as a new method can be applied for the screening of new antibiotics inhibiting bacterial methionyl-tRNA synthetases from natural products.

• 대한토목학회논문집
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• 제43권6호
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• pp.699-708
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• 2023

원자력 발전소는 안전하게 운영될 수 있도록 설계단계에서 자연재해를 포함한 예상 가능한 모든 외부사건들이 고려되고 있다. 그러나 최근에 기후변화로 인해 설계를 초과하는 자연재해의 발생 가능성이 증가하면서 설계 당시에 예측하지 못했던 극한자연재해와 외부사건에 대해 세심한 검토가 요구되고 있다. 본 연구에서는 국내 원전 부지별 잠재적 극한자연재해를 선별하고자 하였다. 이를 위해서 원전 부지특성과 원전 부지별 외부재해로 인해 발생한 사건을 조사하고 분석하였다. 그리고 기존 문헌과 연구 자료를 조사하여 국내 원전 실정에 맞는 선별절차와 기준을 수립하였다. 본 연구에서 수립된 선별기준에 근거하여 원전 부지별로 정성적 선별을 수행하였고, 선별된 자연재해를 대상으로 정량적 선별과 현장실사를 통해 국내 원전 부지별 잠재적 극한자연재해를 선별하였다. 선별 결과, 폭우에 의한 내부침수 이외에도 강풍에 의한 풍압과 극한 공기압이 모든 부지에서 공통적인 잠재적 극한자연재해로 선별되었다. 그리고 고리부지의 경우 폭풍해일이 1순위의 잠재적 극한자연재해로 선정되었다.

• 미생물학회지
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• 제22권3호
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• pp.143-150
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• 1984

The effect of growth inhibitors on colonial growth of cellulolytic fungus, genus Trichoderma, was investigated to develop a method for the effective screening of various auxotrophs and hypercellulolytic strains. As the results, it was revealed that non-ionic detergents such as Sodium deoxycholate and Triton X-100 were the better ones as a growth inhibitor than Oxgall which has been used to restrict the colony size in genus Trichoderma. Each individual colony remained distinct on minimal plate supplemented with 0.05% Triton X-100 for as long as two or more weeks. The screening of 150 to 200 colonies simultaneous on a single plate was possible in the presence of Triton X-100. The effect of Triton X-100 on simultaneous screening was higher than that of Oxgall by a factor of two.

• 한국자원식물학회지
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• 제6권2호
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• pp.155-163
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• 1993

21plants, which collected from Korea and Japan, were applied to antitumor and cytotoxic screening tests against sarcoma 180 a ascitec in mice. The results are summariged as follows : 1) The total packed cell volume method has been used for the antimeoplastic screening for from natural higher plants in Korea. By this method, we have found out that Selaginella involves, Patrinia hispida, Archyranthes japonica and Solanum nigrum having significant activity and also Cydonia sinensis and Rubia akane showed slight activity to antitumor 2) The total packed cell volune method has been used for the antineoplastic screening for from natural higher plants in Japan. Among the 21 tested plants, lsodon japonicus having strong antitumor activity and also Torilis japonica, Aralia elata, Leonurus sibiricus and Rubia cordifolia showed significant activity to anticancer tumor while Forsythia spp and Solanum nigrum showed slight activity to antitumor. 3) Among the 21 tested Korea plants, lsodon excisus and Forsythia Koreana showed strong antitumor activity by the V79 cytotoxic cell screening test.

• 한국자원식물학회지
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• 제5권2호
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• pp.95-103
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• 1992

Present anticancer drugs in the clinical side have not showed a conclusive effect of the chemotherapy for cancer patients. In order to find much more efficient antitumor agents fromnatural resources, various screening methods vivo and in vitro have been developed by manyresearchers. The intention of this paper is to provide an outline of some background on the tumorsystem in drug development of natural products, to review some screening programs for theevaluation of antitumor activity and to introduce the practical procedures of some antitumorscreening methods in vivo and in vitro. At the end of this paper, the current literatures related toantitumor natural products from higher plants at our laboratory are described.Key words'anticancer drugs, screening methods.

• 생약학회지
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• 제8권3호
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• pp.103-108
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• 1977

Phytochemical screening results of forty nine plants in Korea were tabulated in this paper.

• 생약학회지
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• 제13권4호
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• pp.163-165
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• 1982

Phytochemical screening results of sixty nine medicinal plants of Korea were tabulated in this paper.

• 생약학회지
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• 제23권2호
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• pp.117-119
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• 1992

Among the methanolic extracts of thirteen medicinal plants, Aralia elata root and Schrophulariae Radix were verified to have hypoglycemic action in normal and alloxan-induced hyperglycemic rats.

• Journal of Life Science
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• 제9권2호
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• pp.52-56
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• 1999

The Raf, a cytoplasmic serine/thereonine protein kinase, acts as an important mediator of signals involving cell proliferation, differentiation and development. Multiple regulatory elements should participate in the expression of D-raf, Drosophila homolog of human c-raf-1. In order to search regulatory factors involved in the D-raf promoter activation, we accomplished the yeast one-hybrid screening using D-raf promoter region from bp-330 to -309 with respect to the transcription initiation site as bait. After screening, sixteen independent positive clones of ${\beta}$-galactosidase activties were identified and sequenced. Two clones having 94-98% identity with daughterless and one clone having 93% identity with escargot by Blast search among these clones were screened.