• Korean Journal of Clinical Laboratory Science
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• v.48 no.2
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• pp.62-67
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• 2016

Anti-oxidant activity of 600 medicinal plants from Jeju was analyzed. Extracts from the leaves of Distylium racemosum have the highest anti-oxidant activity. D. racemosum is an oriental medicinal plant belonging to the Hamamelidacea and grows in the wild in Jeju. This study was conducted to evaluate the antioxidant and cell viability of different fractions (n-hexane, methylene chloride, ethyl acetate, buthanol, DW) from D. racemosum. Anti-oxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test and total phenolic content. Cell viability was determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) cell viability assay on HepG2 and A549 cells. Among various extracts, the ethyl acetate fraction showed the highest DPPH radical scavenging activity, reaching approximately 93% at 0.5 mg/mL, higher than that of quercetin used as a positive control. Ethyl acetate fractions showed the highest total phenolic content at 505 mg GAE/g. The phenolic content of each extract showed association with DPPH radical scavenging activity. The ethyl acetate extracts were resistant against hydrogen peroxide ($H_2O_2$) treatment in the MTT cell viability assay and showed a higher cell protective effect than other fraction extracts. These results suggest that the ethyl acetate fraction might be a source of anti-oxidants of D. racemosum.

• Journal of the Korean Wood Science and Technology
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• v.38 no.6
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• pp.579-586
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• 2010

This study was carried out to evaluate the antifungal and antioxidative activities of ethanol extracts from Magnoliaceae to investigate the possibility for the natural fungicides and food preservatives. The antifungal activities of ethanol extracts were evaluated as a hyphal growth inhibition rate using four plant pathogenic and five wood rot fungi. The high inhibition activity on the growth of fungi was shown in bark of Magnolia obovata that indicated more than 50% hyphal growth inhibition rate except Trametes versicolor, one of the white rot fungi. The antifungal activity was the highest in the ethanol extracts from M. obovata and the following was in order of M. kobus and M. sieboldii. The extract from bark in M. obovata showed higher antifungal activity than that from wood in the same species. Especially, the extracts from flower of M. denudata and M. liliflora indicated the high antifungal activities, while the other portions of same plants showed the low activities. On the other hand, a free radical scavenging method was adopted with 1,1-diphenyl-2-picrylhydrohydrazyl (DPPH) in order to test the antioxidative activities of ethanol extracts. The free radical scavenging activity was very high in the extracts from branch of Schizandra nigra and Kadsura japonica that showed more than 90% at the concentration of 100 ${\mu}g/m{\ell}$. It also turned out that the antioxidative activity of branch of S. nigra and K. japonica was similar to ${\alpha}$-tocopherol and butylated hydroxytoluene, one of the effective synthetic antioxidants. From these results, it can be suggested that the branches of S. nigra and K. japonica have the positive antioxidative activities and can be applied for the food preservatives and cosmetic ingredient.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.1-6
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• 1997

The experiment was conducted to investigate the effect of water stress on yield and quality of Ligusticum chuanxiong Hort. The water stress treatment was imposed artificially on seedling, flowering and rhizome enlargement stage of the plant. The root yield rate decreased to 19.1%, 18.2% and by the water stress treatment at rhizome enlargement, seedling and flowering stage, respectively. Portion of the products having commercial quality grade (above 20g of rhizome weight) was 93.4% at control plot, while it was 85%, 81.7% and 78.3% when stressed for water at seedling, flowering and root enlargement stage, respectively. Content of extract was the higher in the order of control, water stressed at rhizome enlargement, flowering and the seedling stage. Postive correlationship was found between yield of rhizome and rootlet yield or economic production ratio, and between dry weight of stem and rootlet yield.

• Korean Journal of Medicinal Crop Science
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• v.12 no.1
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• pp.73-78
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• 2004

The study was conducted to develop new materials of functional foods from natural resources. Fortyeight medicinal plants were screened on inhibitory activity of angiotensin-converting enzyme (ACE), a protein elevating blood pressure. The water extracts of Armoracia aerial part, Cynanchum radix, Euonymus branch, Phyllostachys stem, Trichosanthes seed, and methanol extract of Silybum aerial part were showed strong inhibitory activity (above 70%) on ACE at the final concentration of $4,000\;{\mu}g\;ml^{-1}$. Among the plant extracts, water extracts of Phyllostachys taeniam and Trichosanthes seed were especially showed strong inhibitory activity (above 25%) at $500\;{\mu}g\;ml^{-1}$. These two plants are expected good candidate for development into anti-hypertensive materials.

• Journal of Life Science
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• v.27 no.11
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• pp.1262-1268
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• 2017

Lemon myrtle (Backhousia citriodora), a plant in the Myrtaceae family, is native to the semitropical rain-forests of Queensland and is presumably the most commercialized native spice. In Australian thousands of lemon-myrtle trees are under tillage. This study was carried out to investigate the alcohol metabolism, hepatoprotective effects and antidiabetic, tyrosinase inhibitory activity of hot-water (LMW) and 80% ethanol (LME) extracts from lemon-myrtle leaves. The alpha-glucosidase (${\alpha}$-glucosidase) inhibitory activities of the LMW and LME extracts were 7.66% and 40.29% at 1 mg/ml (p<0.05), respectively. The tyrosinase inhibitory activity of the LME extract was about 38.26 % at 1 mg/ml. The effects the LMW and LME extracts had on alcohol-metabolizing activities were determined by measuring the generation of reduced nicotinamide-adenine dinucleotide (NADH) by acetaldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH). The ADH activities of the LMW and LME extracts significantly increased in a dose-dependent manner and were about 154.40% and 192.03% at 1 mg/ml, respectively (p<0.05). The ALDH activities of the LMW and LME extracts also significantly increased in a dose-dependent manner and were about 151.14% and 192.34% at 1 mg/ml, respectively (p<0.05). At $100{\mu}g/ml$, the LMW and LME extracts showed significant protective effects against tacrine-induced cytotoxicity in Hep G2 cells. The results suggested that Backhousia citriodora leaf extracts have the potential to be significant sources for natural health products.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.2 s.51
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• pp.189-196
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• 2005

Motherwort (Leonurus sibiricus L), a hemp nettle (Labiatae) plant, grows about 1.5${\~}$2 m high spontaneously to wildness in Korea, China, Japan, and other Asian place. Its medical applications includes women's uterine disease, urination, bloody stool, bloody urine, and hemorrhoids. It's also effective for high blood pressure, heart stimulation, and anti-cancer activity. We first prepared chlroform and methanol extracts of motherwort and then they were fractionated using water, $30\%$ methanol, $60\%$ methanol, and $100\%$ methanol, respectively. Each fractionates is assayed for free radical scavenging activities against DPPH and anti-oxidant activity by TBARS assay measuring lipid peroxidation using LDL. The $30\%$ and $60\%$ methanol fractionates of methanol extracts showed strong anti-oxidant activity compared to vitamin C. They also had more Potent SOD activity using pyrogallol at 250 ppm than that of vitamin C. These results suggest that anti-oxidant activities of motherwort may be applicable to development of natural anti-oxidant cosmetics. Possibility of nature anti-oxidation ability cosmetics is seen possibility low official.

• Journal of Life Science
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• v.13 no.4
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• pp.421-427
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• 2003

A natural habit at bacterium, Enterobacter intermedious KH410 was isolated from freshwater plant root and identified. Adsorption of heavy metals such as lead, cadmium, and copper by this strain was examined. The minimal inhibitory concentrations(MIC) for each metal were 1.78 mM for lead, 0.17 mM for cadmium and 1.39 mM for lopper, respectively. Maximum production of dried cell was 2.56 g/$\ell$ in LB medium containing 0.5% NaCl, 1% yeast extract and 1% of lactose. Optimal conditions for adsorption were 0.6 dry g-biomass, at pH 4.0 and the temperature of $20^{\circ}C$. Adsorption equilibrium reached maximum after 30 min in 400 mg/$\ell$ metal solution. The adsorption capacity (K) of copper was 1.5 times higher than that of cadmium and lead was 1.1 times higher than that of cadmium. from the results obtained in this study, Freundlich adsorption model was applicable for all metals. Adsorption strength (1/n) of heavy metal ions were in the order of cadmium>copper>lead. The adsorption of dried cell for lead, cadmium, and copper was 56.2, 58.0, 55.8 mg/g-biomass, respectively. Pretreatment to increase ion strength was the most effective with 0.1 M NaOH whereas slight difference was found both KOH and $CaCl_2$ upon same concentration. Effective desorption was induced by 0.1 M EDTA for lead and 0.1 M $HNO_2$ for cadmium and copper.

• Korean Journal of Breeding Science
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• v.40 no.1
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• pp.84-87
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• 2008

Kokang is a high-quality, disease resistant and high-yielding Rehmannia glutinosa cultivar developed by the medicinal crop breeding team of National Institute of Crop Science, RDA, during the period from 1997 to 2005. The reproduction of Rehmania glutinosa has been accomplished mainly by vegetative propagation with its seedlings have many variants. The cultivar was selected from seedling of Jihwang 1. The plant type of Kokang is some rising from ground. It has higher disease resistance, catalpol content and extract content compared with Jihwang 1. Regional yield trials conducted at three site from 2003 to 2005. The root yield of Kokang was 11.8ton per hectare, which was increased 13% compared with a check variety, Jihwang 1. This cultivar is adaptable to the whole of Korea except for mountain areas.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.29 no.2
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• pp.63-72
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• 2011

The aim of this study, a traditional residential area in the environmentally friendly design techniques to identify the techniques and principles that have been carried out to reestablish the principles. To do this, through literature review environmental performance is reflected in the traditional residential area side of resources conservation, locational aspects, spatial configuration, and how cases were selected looking for ways to apply modern. Are examples of upper class housing in the Chosen Dynasty Period period construction relatively well-preserved round and a good building with a clear housing Dokrakdang year were selected. Locational aspects of the terrain with minimal changes to the building and construction techniques were entirely in terms of environmental conservation and environmental temperature was adjusted to regulate the room temperature technique could be seen. In terms of cycling in natural materials were recycled. and water make used of positive through water cycling technique & water control. In addition, the importance of landscape views overlooking the landscape from inside to outside through the regulation of the various internal and external space technique was used to attract and expand. Traditionality in the pursuit of modern space, simply cut off because of tradition rather than to restore or recover the organizing principle inherent in the traditional space, and extraction of the contemporary social, cultural and environmental understanding of space is acceptable in basis. Environmentally-friendly design techniques in a traditional residential area for a long time to be developed by the experience of its application of modern environmental and energy problems and pleasant environment to the creation of human life and are subject to significant swings in that.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.1
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• pp.5-12
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• 2021

In order to extract the essential oil contained in the Elsholtzia splendense Nakai, a fully-bloomed individual was collected and the living body was used for the experiment. Plants were divided into 0.7kg of flowers and 1.5kg of leaves + stems, and extracted by parts and used for scent pattern analysis, and extracted after adding 5kg of outpost for the analysis of essential oil components. Essential oil extraction was performed using the SDE method improved by Schultz et al. (1977), and the extraction time was limited to 1 hour after the material started boiling. The extracted essential oil component was analyzed for fragrance pattern analysis using an e-nose, and the results of analyzing the substance of the essential oil component by GC-MS are as follows. 1. As for the fragrance pattern analysis, in the case of essential oils extracted from flowers, the scent quality was the best at 10-20 minutes, and the scents extracted from stems and leaves were somewhat of poor scent quality, but the fragrance was good at 10-40 minutes. The intensity of scent was the strongest in 10-20 minutes, and the intensity of incense was high even in 30-40 minutes. The scent extracted from the stems and leaves was generally not strong, but appeared high in 10 to 20 minutes. 2. There were 40 kinds of essential oils contained in Elsholtzia splendense Nakai oil. Among them, Mequinol, Benzene, 1,2,3,4-tetramethyl, Elsholtziaketone, and Dehydroelsholtziaketone were identified.