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http://dx.doi.org/10.5352/JLS.2017.27.11.1262

The Hepatoprotective Effects of Hep G2 Cells and the Alcohol-Metabolizing Enzyme Activities of Lemon-Myrtle (Backhousia citriodora) Leaf Extracts  

Jung, Kyung Im (Department of Food & Nutrition, Silla University)
Kim, Pan Kil (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Gal, Sang Wan (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Choi, Young Ju (Department of Food & Nutrition, Silla University)
Publication Information
Journal of Life Science / v.27, no.11, 2017 , pp. 1262-1268 More about this Journal
Abstract
Lemon myrtle (Backhousia citriodora), a plant in the Myrtaceae family, is native to the semitropical rain-forests of Queensland and is presumably the most commercialized native spice. In Australian thousands of lemon-myrtle trees are under tillage. This study was carried out to investigate the alcohol metabolism, hepatoprotective effects and antidiabetic, tyrosinase inhibitory activity of hot-water (LMW) and 80% ethanol (LME) extracts from lemon-myrtle leaves. The alpha-glucosidase (${\alpha}$-glucosidase) inhibitory activities of the LMW and LME extracts were 7.66% and 40.29% at 1 mg/ml (p<0.05), respectively. The tyrosinase inhibitory activity of the LME extract was about 38.26 % at 1 mg/ml. The effects the LMW and LME extracts had on alcohol-metabolizing activities were determined by measuring the generation of reduced nicotinamide-adenine dinucleotide (NADH) by acetaldehyde dehydrogenase (ALDH) and alcohol dehydrogenase (ADH). The ADH activities of the LMW and LME extracts significantly increased in a dose-dependent manner and were about 154.40% and 192.03% at 1 mg/ml, respectively (p<0.05). The ALDH activities of the LMW and LME extracts also significantly increased in a dose-dependent manner and were about 151.14% and 192.34% at 1 mg/ml, respectively (p<0.05). At $100{\mu}g/ml$, the LMW and LME extracts showed significant protective effects against tacrine-induced cytotoxicity in Hep G2 cells. The results suggested that Backhousia citriodora leaf extracts have the potential to be significant sources for natural health products.
Keywords
Acetaldehyde dehydrogenase; alcohol dehydrogenase; ${\alpha}$-glucosidase; hepatoprotective; lemon myrtle;
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