• Korean Institute of Interior Design Journal
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• v.19 no.4
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• pp.22-30
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• 2010

Light has primary function to enlighten the darkness. However, as development and progresses in media has caused big change in our life, the range of usage and application of light is broadened. And these days, light is actively used as a communication tool in a space. This thesis is based on an idea that a new prospect of light is required and at the same time the theoretical foundation should be established to take active advantage of light. In Chapter two, analysis criteria are established to verify study hypothesis. The applied methodology is to analysis the contents based on literature review. Key words related light in space design are extracted and the frequency of those key words are quantitatively measured. Taking advantage of the result, the characteristics of light are divided into three categories, 'light as symbolic information', 'light as communication medium' and 'light as topological form'. In chapter three, analysis of cases is performed. The cases are selected based on space analyzing criteria established in Chapter two. Qualitative analysis is performed on the cases categorized into natural light and artificial light. Basic analysis elements are evaluated for each case. And each case is allocated to one of three categories, 'light as symbolic information', 'light as communication medium' and 'light as topological form'. Through this analysis, it is found that light in space has prevailing characteristics of 'light as symbolic information' and 'light as communication medium'.

• Journal of Applied Biological Chemistry
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• v.56 no.2
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• pp.119-129
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• 2013

Thirteen different Streptomyces isolates were evaluated for their ability to produce keratinase using chicken feather as a sole carbon and nitrogen sources under solid state fermentation (SSF). Streptomyces sp. NRC 13S produced the highest keratinase activity [1,792 U/g fermented substrate (fs)]. The phenotypic characterization and analysis of 16S rDNA sequencing of the isolate were studied. Optimization of SSF medium for keratinase production by the local isolate, Streptomyces sp. NRC13S, was carried out using the one-variable-at-a-time and the statistical approaches. In the first optimization step, the effect of incubation period, initial moisture content, initial pH value of the fermentation medium, and supplementation of some agro-industrial by-products on keratinase production were evaluated. The strain produced about 2,310 U/gfs when it grew on chicken feather with moisture content of 75% (w/w), feather: fodder yeast ratio of 70:30 (w/w), and initial pH 7 using phosphate buffer after 8 days. Based on these results, the Box-Behnken design and response surface methodology were applied to find out the optimal conditions for the enzyme production. The corresponding maximal production of keratinase was about 2,569.38 U/gfs.

• Applied Biological Chemistry
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• v.42 no.3
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• pp.229-234
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• 1999

The effects of linoleic acid(LA, 18 : 2) and/or bovine serum albumin(BSA) on the lipid metabolism in human hepatoma cell line HepG2 cells were evaluated. HepG2 cells were cultured in basal Dulbecco's modified Eagle's(DME) medium(Basal medium), DME medium containing 0.2 mM LA(LA medium), or DME medium containing both 0.2 mM LA and 0.2-1.0% BSA(LA+BSA medium). $[^{14}C]Acetate(0.3\;{\mu}Ci/ml\;medium)$ was added as a radioactive lipid precursor and the cells were incubated for 6 hours. An addition of LA to basal medium resulted in a decrease in the incorporation of $[^{14}C]acetate$ into total cholesterol fraction. In contrast, an addition of BSA to LA-containing medium tended to increase the incorporation of $[^{14}C]acetate$ into total cholesterol. The alteration of cholesterol metabolism in HepG2 cells incubated in LA+BSA medium was attributed by an increase in the incorporation of $[^{14}C]acetate$ into free cholesterol, but not cholesteryl ester fraction. In addition, the secretion of cholesterol was increased by LA+BSA medium, suggesting that BSA stimulates cholesterol secretion. No significant change in the incorporation of $[^{14}C]acetate$ into cellular total lipids was observed among the experimental groups. However, an increased incorporation of $[^{14}C]-labelled$ fatty acid into cellular triacylglycerol and decreased incorporation into phospholipid were observed in cells incubated with LA+BSA medium as compared to those of LA medium. The secretions of $[^{14}C]-labelled$ triacylglycerol, phospholipid, and free fatty acid were also stimulated in HepG2 cells incubated with LA+BSA medium. In conclusion, the present study suggests that in human hepatocytes, LA and BSA influence lipid metabolism, and BSA enhances the secretion of lipids.

• Journal of Applied Biological Chemistry
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• v.58 no.1
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• pp.31-38
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• 2015

The rhizomes of Anemarrhena asphodeloide was extracted with 100% ethanol and concentrated subfractions were separated with medium pressure liquid chromatography-based activity profiling. One compound was isolated from the subfraction 10 through the repeated preparative high performance liquid chromatography (prep-HPLC). According to physico-chemical and spectroscopic data including NMR and MS, the chemical structures of the compound was determined as nyasol (1). Nyasol was exhibited potent inhibitory activity for NO ($IC_{50}:12.5{\mu}g/mL$), tyrosinase ($IC_{50}:12.5{\mu}g/mL$), melanin contents ($IC_{50}:12.5{\mu}g/mL$), thymus and activation-regulated chemokine (TARC) production ($IC_{50}:6.25{\mu}g/mL$). As a result, nyasol has an excellent inflammation-dependent anti-whitening and TARC production activity. It could be used to a large range of functional cosmetics.

• Journal of dental hygiene science
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• v.12 no.3
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• pp.209-215
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• 2012

Somok, the heart wood of Caesalpinia sappan is used in traditional Chinese medicine. This study was performed to investigate the effect of growth and culture conditions of brazilin from C. sappan against S. mutans ATCC 25175. The bacteria were cultured in brain heart infusion (BHI) broth, and then incubated under 5% $CO_2$ at $37^{\circ}C$ for 18-24 hours. The effect of brazilin against S. mutans was confirmed under the changes of the culture conditions, such as growth curve and the change of pH, protein, and total carbohydrate. The growth of S. mutans in control medium was the highest at 24 hr, while brazilin-added medium (0.3 mg/ml) showed maximum growth at 32 hr. The pH values of the control medium was 5.25 at 16 hr, but the media supplemented with brazilin (0.3 mg/ml) was 7.0 at 16 hr. The amounts of total carbohydrate of the control medium was 11 mg/ml at 8 hr, but the brazilin-added media (0.3 mg/ml) was 18 mg/ml at 8 hr. In the protein change of the culture medium, the control culture broth and the brazilin supplemented-cultures was 2.4 mg/ml and 2.54 mg/ml at 24 hr, respectively. Polysaccharide contents of the control medium and test media were 3 mg/ml and 2 mg/ml at 8 hr, respectively. Thus, the application of C. sappan can be considered a useful and practical material for the prevention of dental caries.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.151.1-151.1
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• 2010

Natural gas hydrates (NGH) provide 170 gas volumes per unit volume of the medium and are easier to make with moderate pressure and temperature (40 bar at 3 C). Once they form, their preservation temperature is 20 C at 1 bar, which is much milder than the LNG preservation. In case of using the NGH, The small and medium sized gas well has advantages for development because of NGH's these characteristics. According to the cost evaluation report of Gudmundsson in Norway and the research of MES in Japan, the gas well that uses the NGH has a cost saving effect about 10~20% compared LNG. The effect depends on distance and production. However, cost saving and efficiency of liquefaction process is increased by the development of LNG liquefaction technology. Therefore, these factors have to be reflected in economic analysis. The purpose of this research is to compare the cost of Gas Supply Chain according to the transport type, distance and gas reserves. Especially, we consider not only the cost of facility but also the total cost (production cost, transport cost, etc).

• Journal of Life Science
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• v.22 no.6
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• pp.723-729
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• 2012

Filter plate microbial trap (FPMT) was invented as an in situ cultivation device for the isolation of bacteria from natural environments. FPMT consists of a medium and membrane filters (0.45 ${\mu}m$ pore size) and microorganisms and compounds can be moved freely moved into the medium. This device was applied to two soil samples of Greenland. The microbial diversity of both soil samples by FPMT was higher than that by the conventional Petri dish-based method. Moreover, novel bacterial species were isolated by FPMT. The new FPMT is effective for in situ cultivation of natural samples and could be applicable to the isolation of uncultivable microorganism.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.05a
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• pp.832-835
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• 2005

The free vibration of tapered piles embedded in soil is investigated. The pile model is based on the Bernoulli-Euler beam theory and the soil is idealized as a Winkler model for mathematical simplicity. The governing differential equations for the free vibrations of such members are solved numerically. The square tapered piles with one free and the other hinged end with rotational spring are applied in numerical examples. The lowest two natural frequencies are obtained over a range of non-dimensional system parameters: the rotational spring parameter, the embedded ratio, the foundation parameter, the width ratio of the contact area and the section ratio.

• Structural Engineering and Mechanics
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• v.68 no.1
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• pp.103-119
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• 2018

In the present research, an attempt is made to obtain a semi analytical solution for both nonlinear natural frequency and forced vibration of embedded functionally graded double layered nanoplates with all edges simply supported based on nonlocal strain gradient elasticity theory. The interaction of van der Waals forces between adjacent layers is included. For modeling surrounding elastic medium, the nonlinear Winkler-Pasternak foundation model is employed. The governing partial differential equations have been derived based on the Mindlin plate theory utilizing the von Karman strain-displacement relations. Subsequently, using the Galerkin method, the governing equations sets are reduced to nonlinear ordinary differential equations. The semi analytical solution of the nonlinear natural frequencies using the homotopy analysis method and the exact solution of the nonlinear forced vibration through the Harmonic Balance method are then established. The results show that the length scale parameters give nonlinearity of the hardening type in frequency response curve and the increase in material length scale parameter causes to increase in maximum response amplitude, whereas the increase in nonlocal parameter causes to decrease in maximum response amplitude. Increasing the material length scale parameter increases the width of unstable region in the frequency response curve.

• Journal of the Korean Institute of Landscape Architecture
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• v.29 no.5
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• pp.20-27
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• 2001

The purpose of this study is to investigate and compare the validity and the reliability of the visual simulation method using the internet. For this. the evaluation of the artificial and natural landscape through the medium of color slides are compared with the internet survey. Data is analysed through the comparison of t-test between the two media by landscape type, and spatial image is analysed by factor analysis algorithm. Principle component analysis using Varimax Method is applied for extraction and factor rotation respectively. The results of this study can be summarized as follows; There are no statistical differences between the two methods with artificial and natural landscape in the total data that included second tests. Factors covering the spatial image are found to be \`aesthetic\`, \`spatial shape\`, and \`familiarity\`. Total variance is obtained as 66.4%. There are no statistical differences between the two methods in 2/3 of the cases. In the case of far view of artificial landscape, the results of the t-test show that the two methods are exactly the same. Especially in the case of the artificial far landscape shows no difference of all factors between two methods. There are no differences between first and second tests of the same media and the same landscape type. And it shows the reliability of this method. These results suggest that the probability that the internet can be used as a medium of landscape evaluation and gathering information on anyone\`s landscape image. Simulation techniques with the internet survey method should be further developed for practical application.