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http://dx.doi.org/10.5352/JLS.2012.22.6.723

Filter Plate Micro Trap as a Device for in situ Cultivation for Environmental Microorganisms  

Jung, Da-Woon (Department of Environmental Science, Kangwon National University)
Ahn, Tae-Seok (Department of Environmental Science, Kangwon National University)
Publication Information
Journal of Life Science / v.22, no.6, 2012 , pp. 723-729 More about this Journal
Abstract
Filter plate microbial trap (FPMT) was invented as an in situ cultivation device for the isolation of bacteria from natural environments. FPMT consists of a medium and membrane filters (0.45 ${\mu}m$ pore size) and microorganisms and compounds can be moved freely moved into the medium. This device was applied to two soil samples of Greenland. The microbial diversity of both soil samples by FPMT was higher than that by the conventional Petri dish-based method. Moreover, novel bacterial species were isolated by FPMT. The new FPMT is effective for in situ cultivation of natural samples and could be applicable to the isolation of uncultivable microorganism.
Keywords
Cultivation device; in situ cultivation; microbial diversity; uncultivable bacteria;
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