• 자원환경지질
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• 제49권1호
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• pp.23-30
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• 2016

자연산 로소나이트(Ca-Lawsonite, $CaAl_2Si_2O_7(OH)_2{\cdot}H_2O$) 분말의 압력증가에 따른 변화와 구조적 특성을 소형 압력 발생장치인 다이아몬드 앤빌셀과 실시간 싱크로트론 X-선 회절실험을 통하여 확인하였다. 본 연구에서 시료에 적용한 압력은 1 기압에서 8 GPa 까지였으며 메탄올, 에탄올, 물의 혼합 용액을 압력 매개체로 하여 상온조건에서 실험하였고, 특이한 상전이나 압력하 부피변화의 이상현상은 발견되지 않은 가역적 압축특성을 관찰하였다. 최고압력까지 로소나이트는 결정성을 잃지 않는 것으로 확인되었고 압력증가에 따른 체적감소를 통해 체적탄성률($B_0$)을 계산해 본 결과 146(6) GPa로 도출되었다. 체적탄성률과 함께 계산된 선형압축률은 a-, b-, c-축에서 각각 0.0022, 0.0024, $0.0020GPa^{-1}$로 압력에 의한 부피감소는 대체적으로 3축이 같은 비율로 감소하는 것을 확인하였다.

• 한국농공학회지
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• 제20권4호
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• pp.4805-4811
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• 1978

This paper deals with the correlations between the results of the physical property tests and the mechanical property tests for estimating the mechanical indexes by the physical property test results. The soil samples were taken at changweon area, Gyeon-gsangnam-do, where the structures would be placed on the alluvial clay foundation. The outcomes of the study are as follows: 1. Judging from casagrande's plasticity chart, it is considered that the almost all soil samples belong to inorganic silty clay with medium plasticity (clay 14∼62%, silt 36∼73%, sand 1∼29%). The specific gravities are between 2.61 and 2.72, the wet unit weights 1.53g/㎤ and 1.93g/㎤, the liquid limits 28% and 51%, the plastic limits 15% and 31%, the plastic indexes 7% and 27%, the natural moisture contents 33% and 64%. 2. The unconfined compression strengths are between 0.07kg/$\textrm{cm}^2$ and 0.77kg/$\textrm{cm}^2$, the cohesions 0.04kg/$\textrm{cm}^2$ and 0.37kg/$\textrm{cm}^2$, the internal friction angles 0$^{\circ}$ and 9$^{\circ}$. 3. The consolidation tests show that the initial void ratios are between 0.68 and 1.68, the precompression loads 0.27kg/$\textrm{cm}^2$ and 1.15kg/$\textrm{cm}^2$, the compression indexes 0.12 and 0.59. 4. The correlations between the results of the physical property tests and the mechanical property tests for the soil samples are presented as follows: rt=0.011 (203-wn), Cc=0.025 (LL-27.2), Cc=0.46 (e0-0.58), Cc=0.013 (wn-23.2), C=0.021+qu/2.08, qu=2.268rt-3.635

• 한국균학회지
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• 제38권1호
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• pp.80-82
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• 2010

화경버섯은 동아시아 지역에서 독버섯으로 알려져 있지만, 최근에 Illudin S가 항암효과가 있는 것으로 보고되었다. 이 버섯은 자연조건에서 매우 드물게 발견되는 버섯이다. 그래서 우리는 미래에 약리학적 연구를 위해 충분한 양의 버섯을 얻고자 이에 대한 톱밥재배를 시도하였다. 버섯은 실험한 모든 톱밥배지에서 수확할 수 있었고, 버섯 생산성과 재배기간을 고려할 때 화경버섯의 재배는 소나무 톱밥배지를 이용하는 것이 가장 좋은 것으로 나타났다. 버섯은 600 g 배지당 평0균 43~80 g이 생산되었고, 재배기간 동안 1~3회 버섯을 수확할 수 있었다.

• 보존과학연구
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• 통권32호
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• pp.99-112
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• 2011

함양 승안사지 삼층석탑은 중립 내지 세립질의 흑운모화강암과 화강편마암이 주를 이루고 있으며 부분적으로 거정질편마암, 백운모편마암, 반려암질암이 혼용되었다. 이 석탑은 주변의 수목 및 잔디에 의해 전면에 걸쳐 지의류와 선태류 등이 서식하고 있어 석탑 표면이 심하게 훼손되어 있다. 따라서 이 연구에서는 이 석탑에 대한 종합적인 풍화훼손도 진단과, 이를 근거로 보존처리를 수행하였다. 보존처리에서는 석탑의 전면에 걸쳐 자생하는 지의류 및 오염물 등에 대해 부드러운 솔과 나무칼을 사용하여 건식세정을 실시하였고 이때 고착지의류 등 잘 제거되지 않는 부분은 증류수를 이용한 습식세정으로 제거하였다. 또한 기존에 설치되어 있는 보호난간을 석탑과의 거리를 고려하여 적당한 높이로 재설치하였으며, 석탑 주변의 지반은 마사토로 정비하고 배수흐름을 원활하게 하기위해 자연구배를 두고 자연측구를 설치하였다.

• Journal of Microbiology
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• 제37권4호
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• pp.248-255
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• 1999

We have analyzed the MRE3/REC114 gene of Saccharomyces cerevisiae, previously detected in isolation of mutants defective in meiotic recombination. We cloned the MRE3/REC114 gene by complementation of the meiotic recombination defect and it has been mapped to chormosome XIII. The DNA sequence analysis revealed that the MRE3 gene is identical to the REC114 gene. The upstream region of the MRE3/REC114 gene contains a T_4C site, a URS (upstream repression sequence) and a TR (T-rich) box-like sequence, which reside upstream of many meiotic genes. Coincidentally, northern blot analysis indicated that the three sizes of MRE3/REC114 transcripts, 3.4, 1.4 and 1.2 kb, are induced in meiosis. A less abundant transcript of 1.4 kb is detected in both mitotic and meiotic cells, suggesting that it is needed in mitosis as well as meiosis. To examine the role of the MRE3/REC114 gene, we constructed mre3 disruption mutants. Strains carrying an insertion or null deletion of the MRE3/REC114 gene showed slow growth in nutrient medium and the doubling time of these cells increased approximately by 2-fond compared to the wild-type strain. Moreover, the deletion mutant (${\delta}$mre3) displayed no meiotically induced recombination and no viable spores. The mre3/rec114 spore lethality can be suppressed by spo13, a mutation that causes cells to bypass reductional division. The double-stranded breaks (DSBs) which are involved in initiation of meiotic recombination were not detected in the analysis of meiotic chromosomal DNA from the mre3/rec114 disruptant. From these results we suggest that the MRE3/REC114 gene product is essential in normal growth and in early meiotic stages involved in meiotic recombination.

• 동의생리병리학회지
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• 제26권1호
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• pp.59-66
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• 2012

Oxidative stress has been implicated in cutaneous damage in various inflammatory skin diseases, including atopic dermatitis. The present study was undertaken to investigate the antioxidative and anti-inflammatory activities of the extract of Duchesnea chrysantha (DCE). DEC was prepared by extracting with 80% ethanol. Total flavonoids and polyphenols were measured by a colorimetric assay. The free radical scavenging activity of the extract was analyzed by the DPPH (1,1-diphenyl-2-picryl hydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and Griess reagent assay. An oxidative product of nitric oxide (NO), was measured in the culture medium by the Griess reaction. The level of prostaglandin $E_2$ ($PGE_2$) was measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were measured by Western blot analysis. Total flavonoid and polyphenol contents of DCE were included $24.73{\pm}0.45$ and $178.77{\pm}2.65$, respectively. DCE significantly increased electron donating ability (DPPH), nitrite scavenging (NO) and ABTS reducing activity in dose dependant. We investigated the anti-inflammatory effects of DCE on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. DCE significantly suppressed NO and prdstaglandin $E_2$ ($PGE_2$) in dose dependant. Furthermore, the levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with DCE in a dose dependent manner. These results suggest that DEC may has value as natural product with its high quality functional components, antioxidative and anti-inflammatory activities.

• Archives of Pharmacal Research
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• 제20권6호
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• pp.533-538
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• 1997

Biflavonoid is one of unique classes of naturally-occurring bioflavonoid. Previously, certain biflavonoids were found to possess the inhibitory effects on phospholipase $A_2$ activity and lymphocytes $ proliferation^1$ suggesting their anti-inflammatory/immunoregulatory potential. In this study, effects of several biflavonoids on arachidonic acid release from rat peritoneal macrophages were investigated, because arachidonic acid released from the activated macrophages is one of the indices of inflammatory conditions. When resident peritoneal macrophages labeled with $[^{3}H]$arachidonic acid were activated by phorbol 12-myristate 13-acetate(PMA) or calcium ionophore, A23187, radioactivity released in the medium was increased approximately 4.1-7.3 fold after 120 min incubation compared to the spontaneous release in the control incubation. In this condition, biflavonoids (10 uM) such as ochnaflavone, ginkgetin and isoginkgetin, showed inhibition of arachidonate release from macrophages activated by PMA (32.5-40.0% inhibition) or A23187 (21.7-41.7% inhibition). Amentoflavone showed protection only against PMA-induced arachidonate release, while apigenin, a monomer of these biflavonoids, did not show the significant inhibition up to 10 uM. Staurosporin (1 uM), a protein kinase C inhibitor, showed an inhibitory effect only against PMA-induced arachidonate release (96.8% inhibition). Inhibition of arachidonate release from the activated macrophages may contribute to an anti-inflammatory potential of biflavonoids in vivo.

• 한국컴퓨터정보학회논문지
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• 제26권9호
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• pp.27-35
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• 2021

인공생명체 연구는 자연 생명과 관련된 시스템이나 그 과정들, 진화 등을 평가해 다양한 응용과학 분야에 활용된다. 이러한 인공생명체의 원활한 활동을 위해 물리적 신체 설계와 행동 제어전략을 진화시키는 연구가 활발히 진행되었다. 그러나 형태와 신경망을 공진화시키는 것은 어렵기에 최적화된 움직임을 가진 인공생명체는 한 가지 형태에 한 가지 움직임만을 가지며 주변 환경 상황은 고려하지 않는 것이 대부분이다. 본 논문에서는 포식자-피식자 모델을 이용하여 형태와 신경망을 공진화하는 인공생명체가 환경적응형 움직임을 갖게 한다. 그런 다음 포식자-피식자 계층 구조를 최상위 포식자-중간 포식자-최하위 피식자 3단계로 확장하여 초기 개체군 밀도에 따라 시뮬레이션의 안정성을 판별하며 형태 진화와 개체군 역학 간의 상관관계를 분석한다.

• Nuclear Engineering and Technology
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• 제53권7호
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• pp.2207-2220
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• 2021

Lead alloy is used as coolant in Lead-based cooled Fast Reactor (LFR). The natural characteristics of lead alloy are combined with the simple structural design of LFR. This constitutes the inherent safety characteristics of LFR. The main work of this paper is to take the main coolant pump (MCP) in the lead-cooled fast reactor (LFR) as the research object, and to study the flow pattern distribution of the internal flow field under the reverse rotation pump condition, the reverse rotation positive-flow braking condition and the reverse rotation negative-flow braking condition. In this paper, the double-outlet volute type and the space guide vane are selected as the potential designs of the CLEAR-I MCP. In this paper, the CFD method is used to study the potential reverse accident of the MCP. It is found that the highest flow velocity in the impeller appears at the impeller outlet, and the Q-H curves of the two design programs basically coincide. The space guide vane type MCP has better hydraulic performance under the reverse rotation positive-flow condition, the Q-H curves of the two designs gradually separate with increasing flow rate, and the maximum flow velocity inside the space guide vane type MCP is obviously lower than that of the double-outlet volute type. For the reverse rotation test of MCP, only the condition of the forward rotating pump of the main coolant pump is tested and verified. For the simulation of the MCP in LBE medium, it proved that the turbulence model and basic settings selected in the simulation are reliable.

• Journal of Plant Biotechnology
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• 제48권4호
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• pp.255-263
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• 2021

Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal communities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl₂ (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low temperature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 ℃; thus, they could be useful for transfer and exchange of P. foetida germplasm.