• Bulletin of the Korean Chemical Society
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• 제19권6호
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• pp.689-695
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• 1998

Carbobenzoxy-alanyl-thiaarginine benzyl ester and carbobenzoxy-alanyl-thialysine benzyl ester were synthesized in solution. Kinetic studies were carried out using three different analytical methods, semi-classical method, progress curve analysis and competitive spectrophotometry. In competitive spectrophotometry, carbobenzoxy-valyl-glycyl-arginyl-p-nitroaniline was used as a detector. Kinetic constants such as $K_m$ and $V_{max}$ measured by competitive spectrophotometry are almost the same as those values measured by semi-classical method. Colorimetric Ellman's assays showed the thio-peptido mimetics to be a suitable substrates for trypsin. Kinetic studies with trypsin gave $K_m$ of 2.33 mM and $k_{cat}$ of $1.50{\times}10^5\;min^{-1}$ for carboxy-alanyl-thiaarginine benzyl ester and $K_m$ of $3.41{\times}10^{-3}\; Mm\; and\; k_{cat}\; of\; 520{\times}102\; min^{-1}$ for carbobenzoxy-alanyl-thialysine benzyl ester, respectively. Kinetic constants $(K_m=2.04{\times}10^{-2}\; mM, K_{cat}=4.42{\times}10^3 \;min^{-1})$ for natural substrate, carbobenzoxy-alanyl-lysine benzyl ester, were also evaluated by competitive spectrophotometry in order to compare the mode of binding on trypsin.

• Journal of Mechanical Science and Technology
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• 제20권4호
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• pp.467-472
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• 2006

A study of the natural vibrations of beam resting on elastic foundation with finite number of transverse open cracks is presented. Frequency equations are derived for beams with different end restraints. Euler-Bernoulli beam on Pasternak foundation and Euler-Bernoulli beam on Pasternak foundation are investigated. The cracks are modeled by massless substitute spring. The effects of the crack location, size and its number and the foundation constants, on the natural frequencies of the beam, are investigated.

• 대한기계학회논문집
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• 제18권8호
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• pp.1899-1909
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• 1994

This work presents the experimental and FEM results for the free vibration of cantilevered, symmetrically and antisymmetrically laminated composite rectangular plates. The natural frequencies, mode shapes and contour plots of a number of CFRP, GFRP, DFRP-Aluminum, GFRP-Aluminum and DFRP-GFRP hybrid composite plates are experimentally obtained. Determination of Young's modulus and test procedures are described. The natural frequencies are determined for a wide range of parameters : e.g. , composite material constants, fiber angles and stacking sequences. Natural frequency and nondimensional frequency parameter results are compared with the finite element analysis and existing literatures. Agreement between experimental and calculated frequencies is excellent. The effects of varing the parameters upon the free vibration frequencies and mode shapes are discussed.

• 호남수학학술지
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• 제34권2호
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• pp.241-252
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• 2012

Let $\{X_{ni},\;1{\leq}i{\leq}n,\;n{\geq}1\}$ be a sequence of LNQD which are dominated randomly by another random variable X. We obtain the complete convergence and almost sure convergence of weighted sums ${\sum}^n_{i=1}a_{ni}X_{ni}$ for LNQD by using a new exponential inequality, where $\{a_{ni},\;1{\leq}i{\leq}n,\;n{\geq}1\}$ is an array of constants. As corollary, the results of some authors are extended from i.i.d. case to not necessarily identically LNQD case.

• Journal of Advanced Marine Engineering and Technology
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• 제30권6호
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• pp.716-723
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• 2006

A meso-scale analysis method using the natural element method is proposed for the analysis of material damage of brittle microcracking solids. The microcracking is assumed to occur along Voronoi edges in the Voronoi diagram generated using the nodal points as the generators. The mechanical effect of microcracks is considered by controlling the material constants in the neighborhood of the micorcracks. The macro elastic moduli of isotropic solids containing a number of randomly distributed microcracks are calculated considering the effect of microcrack closure to demonstrate the validity of the proposed method.

• Journal of Advanced Marine Engineering and Technology
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• 제30권6호
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• pp.724-730
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• 2006

Fracture behavior of brittle solids is closely related to microcracking. A meso-scale analysis method using the natural element method is proposed for the analysis of brittle microcracking solids. The microcracking is assumed to occur along Voronoi edges in the Voronoi diagram generated using the nodal points as the generators. The mechanical effect of microcracks is considered by controlling the material constants in the neighborhood of the microcracks. The meso-analysis method is applied to the simulation of the microcracking behaviors of brittle solids subjected to tensile macrostress. The method is also applied to the analysis of the propagation of a macrocrack accompanied by the coalescence with microcracks formed near the macrocrack-tip.

• 산업기술연구
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• 제16권
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• pp.131-137
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• 1996

For a large scale civil and architectural structures, mainly steel, concrete and aluminum have been used and weight and corrosion of materials became a major concern. Designing with composite materials is very much complicated. Simple classical theory may yield good results for selecting "initial" sections for preliminary design. D. H. Kim proposed to use the quasi-isotropic constants by Tsai for the preliminary design of the composite primary structures for the civil construction. Also he made simple equation using correction factor. In this paper, the simple formulas developed by D. H. Kim to obtain "exact" values of the natural frequencies of [ABBCAAB]r laminate are compared with Whitney's equations. Also natural frequencies of the plate with varying aspect ratios and reinforcing fiber orientations, are compared with natural frequencies of bean. This work can be a guideline to obtain data in many other cases.

• 산업기술연구
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• 제19권
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• pp.227-233
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• 1999

Simple equations which can predict "exact" values of the natural frequency of vibration for the special orthotropic laminates are presented. Many laminates with certain orientations have decreasing values of $B_{16}$ and $B_{26}$ as the number of plies increases. Such laminates, with $D_{16}=D_{26}{\rightarrow}0$, including the laminates with anti-symmetric configurations can be solved by the same equation for the special orthotropic laminates. If the quasi-isotropic constants are used, the equations for the isotropic plates can be used. Use of some coefficients cab produce "exact" value for laminates with such configurations. Natural frequencies of the plate with varying aspect ratios is presented.

• 한국자기공명학회논문지
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• 제25권1호
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• pp.1-7
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• 2021

Employing of 13C stable-isotopes in NMR metabolomics can give unique splitting patterns and coupling constants information originated from 13C-13C coupling interaction that provide an important structural information regarding the cellular metabolic process. But it has been known that an undesirable signal distortion in 2D heteronuclear correlation study, due to 13C-13C interaction, hampers an analysis of the coupling information. Recently, we proposed J-scaled distortion-free heteronuclear single-quantum coherence (HSQC) sequence which provides a distortion-free 13C-13C coupling information with a selective resolution enhancement of JCC splitting. In this paper, we dicuss theoretical aspect and practical feature of J-scaled HSQC pulse sequence. The conceptual explanation of orgin of the signal distortion by 13C-13C coupling interaction and design of J-scaled HSQC through exemplified results are provided in brief.

• 한국자기공명학회논문지
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• 제18권2호
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• pp.52-57
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• 2014

The TCP domain is a DNA-binding domain present in plant transcription factors and plays important roles in various biological functions. The hydrogen exchange rate constants of the imino protons were determined for the three DNA duplexes containing the DNA-binding sites for the TCP11, TCP15, and TCP20 transcription factors using NMR spectroscopy. The M11 duplex displays unique hydrogen exchange property of the five base pairs in the first binding site (5'-GTGGG-3'). However, the M15 and M20 duplexes lead to clear changes in thermal stabilities of these five base pairs. The unique dynamic features of the five base pairs in the first binding site might play crucial roles in the sequence-specific DNA binding of the class I TCP transcription factors.