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Comparison of Glucosinolate Contents in Leaves and Roots of Radish (Raphanus spp.)

  • Ko, Ho-Cheol;Sung, Jung-Sook;Hur, On-Sook;Baek, Hyung-Jin;Jeon, Young-ah;Luitel, Binod Prasad;Ryu, Kyoung-Yul;Kim, Jung-Bong;Rhee, Ju-Hee
    • Korean Journal of Plant Resources
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    • v.30 no.6
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    • pp.579-589
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    • 2017
  • Glucosinolates (GSLs), beneficial secondary metabolites for human health are abundantly present in radish vegetable. Radish is a member of Brassicaceae family and its seed, leaf and root contain very important GSLs. The objective of this study was to determine the variation of individual and total GSL contents in leaves and roots of 44 radish (Raphanus spp.) germplasm (26 R. sativus L., 3 R. raphanistrum, and 15 R. sativus L. var. raphanistroides Makino), and compare the GSL contents between leaves and roots among three Raphanus species. Thirteen GSLs were identified, being the glucoraphasatin (GRS) and glucobrassicin (GBS) the most abundant aliphatic and indolyl GSLs in both the leaves and roots. Variation in individual and total GSL contents was found among the germplasm of three Raphanus species. The GRS content was higher in roots than that of leaves in all three Raphanus species but the GBS content was higher in leaves than roots. GRS was represented 87.0%, 92.7% and 94.7% of the total GSL in roots of R. sativus L., R. raphanistrum and R. sativus L. var. raphanistroides (Makino) germplasm, respectively. Germplasm of R. raphanistrum exhibited the highest (average, $79.5{\mu}mol/g\;dw$) total GSL with a ranged from 62.7 to $92.9{\mu}mol/g\;dw$. The germplasm IT119288, Joseonmu and IT119262 from R. sativus L., RA 504 and K046542 from R. raphanistrum, and Gyeongju-2003-32 (G2003-32) and IT302373 from R. sativus L. var. raphanistroides (Makino) had high total GSL contents and these could be good candidates for developing the functional compounds-rich varieties in radish breeding program.

In vitro Root Induction from Shoot Explants of Pear (Pyrus spp.)

  • Jae-Young Song;Jinjoo Bae;Woohyung Lee;Jung-Ro Lee;Mun-Sup Yoon
    • Korean Journal of Plant Resources
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    • v.35 no.6
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    • pp.770-777
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    • 2022
  • The main objective of this study was to identify the most appropriate condition for root formation of in vitro micropropagated pear (Pyrus spp.) plants. In vitro propagation was induced on Murashige and Skoog (MS) medium with 2.0 mg/L of N6-benzyladenine (BA) and 0.2 mg/L of Indole-3-butyric acid (IBA) medium. The short pre-treatment of explants with a high concentration (1 mg/L) of NAA and IBA (R0 medium) in dark for three days, followed by transfer to five different media (R1 to R5) resulted in good rooting responses in the pear 'Oharabani (P. pyrifolia × P. communis)' genotype. For the rooting experiments, the highest rooting percentage (83.3 ± 8.3%), average root length (3.6 ± 1.9 mm), total root number (31 ± 4.0), and average root number per plant (2.6 ± 2.1) were obtained on half strength (1/2) of MS medium supplemented with 30 g/L sucrose without hormones and activated charcoal (AC) (R1 medium). The highest rooting percentage was obtained at 83.3% from explants on R1 and R3 media. The rooting procedure described in this study resulted in good root formation and significantly shorting the root induction time to within 14 days of culture. Further studies are underway to test the suitability of the protocol developed in this study for other pear genotypes.

Development of Easy Equation for Crop Water Stress Index (CWSIEE) Using the Temperature Difference between Canopy and Air (Tc-Ta) of Fruit Trees (엽온과 기온의 차이를 이용한 노지 과수의 작물 수분 스트레스 지수 산정 간편식 개발)

  • Choi, Yonghun;Lee, Sangbong;Kim, Minyoung;Kim, Youngjin;Jeon, Jonggil;Park, Jeonghun
    • Journal of The Korean Society of Agricultural Engineers
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    • v.62 no.5
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    • pp.85-91
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    • 2020
  • In order to calculate the Crop Water Stress Index (CWSI), it is necessary to collect weather data (air temperature, humidity, wind speed and solar radiation) and canopy temperature. However, it is not always available to have necessary data sets for CWSI calculation. Therefore, this study was aimed to develop an easy and simple CWSI equation (CWSIEE) using only two data, air and canopy temperatures. Infrared sensors and weather sensors were installed on apple and peach trees and nearby a study area and every ten-minute data were collected from June to October in 2018 and 2019, respectively. A relationship between air-canopy temperature difference and CWSI was statistically analyzed and used to develop CWSIEE using the three dimensional Gaussian model. The performance of CWSIEE against original CWSI showed R2 and NSE to 0.780 and 0.710 for apple trees and R2 and NSE to 0.884 and 0.866 for peach trees. This study found that the level of crop water stress could be easily calculated using CWSIEE with only air and canopy temperature data.

Challenges; Interoperability of UAV in the NAS (국가공역체계에서 무인항공기 통합운영에 대한 동향)

  • Kim, Joong-Wook;Kim, Do-Hyun
    • Current Industrial and Technological Trends in Aerospace
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    • v.8 no.2
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    • pp.136-144
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    • 2010
  • The utilization of UAV and the activities of diverse widening, now the challenge was how to operate and integrate UAV safely in the NAS. The purpose of this study is to look around the trend for interoperability of UAV in the NAS and to provide its implications and the future direction of integrated operating airspace focusing on U.S. where R&D and demand of UAV are the most in the world.

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Net Analyte Signal-based Quantitative Determination of Fusel Oil in Korean Alcoholic Beverage Using FT-NIR Spectroscopy

  • Lohumi, Santosh;Kandpal, Lalit Mohan;Seo, Young Wook;Cho, Byoung Kwan
    • Journal of Biosystems Engineering
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    • v.41 no.3
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    • pp.208-220
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    • 2016
  • Purpose: Fusel oil is a potent volatile aroma compound found in many alcoholic beverages. At low concentrations, it makes an essential contribution to the flavor and aroma of fermented alcoholic beverages, while at high concentrations, it induced an off-flavor and is thought to cause undesirable side effects. In this work, we introduce Fourier transform near-infrared (FT-NIR) spectroscopy as a rapid and nondestructive technique for the quantitative determination of fusel oil in the Korean alcoholic beverage "soju". Methods: FT-NIR transmittance spectra in the 1000-2500 nm region were collected for 120 soju samples with fusel oil concentrations ranging from 0 to 1400 ppm. The calibration and validation data sets were designed using data from 75 and 45 samples, respectively. The net analyte signal (NAS) was used as a preprocessing method before the application of the partial least-square regression (PLSR) and principal component regression (PCR) methods for predicting fusel oil concentration. A novel variable selection method was adopted to determine the most informative spectral variables to minimize the effect of nonmodeled interferences. Finally, the efficiency of the developed technique was evaluated with two different validation sets. Results: The results revealed that the NAS-PLSR model with selected variables ($R^2_{\upsilon}=0.95$, RMSEV = 100ppm) did not outperform the NAS-PCR model (($R^2_{\upsilon}=0.97$, RMSEV = 7 8.9ppm). In addition, the NAS-PCR shows a better recovery for validation set 2 and a lower relative error for validation set 3 than the NAS-PLSR model. Conclusion: The experimental results indicate that the proposed technique could be an alternative to conventional methods for the quantitative determination of fusel oil in alcoholic beverages and has the potential for use in in-line process control.

Response of Crop Water Stress Index (CWSI) and Canopy Temperature of Apple Tree to Irrigation Treatment Schemes (관개수준별 사과나무의 엽온 및 수분 스트레스 지수 변화 분석)

  • Kim, Minyoung;Choi, Yonghun;Cho, Junggun;Yun, Seokkyu;Park, Jeonghun;Kim, Youngjin;Jeon, Jonggil;Lee, Sangbong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.61 no.5
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    • pp.23-31
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    • 2019
  • Crop response to weather and internal water pressure changes is more sensitive to crop water stress than soil water content. Recently, its implementation to optimal irrigation scheduling has been receiving much attention. This study was conducted to determine and compare the theoretical crop water stress index (CWSI) using meterological data and canopy temperature collected from three different irrigation treatments, which were Tr-1 plot (rainfed), Tr-2 plot (50% of daily evapotranspiration (ET) irrigated) and Tr-3 plot (75% of daily evapotranspiration (ET) irrigated). The readings of canopy temperature and CWSI were significantly different among irrigation treatment schemes. The average canopy temperatures and CWSIs of Tr-1 and Tr-3 plots were $34.6^{\circ}C$ and $32.6^{\circ}C$, 0.79 and 0.64, respectively. Solar radiation had the biggest correlation with CWSI (R=0.68) which was followed by wind speed, relative humidity and air temperature. Overall, the findings of this study indicated that canopy temperatures and CWSIs could be further used for irrigation scheduling for crop growth.

Complete Genome Sequence of Pseudarthrobacter sp. IC2-21, a Fluquinconazole-Degrading Soil Bacterium

  • Myoungjoo Riu;Songhwa Kim;Jaekyeong Song
    • Microbiology and Biotechnology Letters
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    • v.52 no.1
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    • pp.94-96
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    • 2024
  • Pseudarthrobacter sp. IC2-21 is isolated from the greenhouse soil in Icheon, Gyeonggi, Korea. This strain IC2-21 is a first fluquinconazole degrading soil bacterium. We analyze the whole genome sequence of Pseudarthrobacter sp. IC2-21. The sequence analysis revealed that Pseudarthrobacter sp. IC2-21 possesses a single 4,265,009 bps circular chromosome with a DNA G+C-content of 65.4%. This chromosome contains 3,942 protein-coding sequences and 12 rRNA and 51 tRNA genes. In the result of sequence analysis, it is revealed that strain IC2-21 possessed genes coding the triazole pesticides degradation related enzymes, such as oxygenase, and fluquinconazole degradation related genes.

A Study on Operability of Smart UAV in the NAS (스마트무인기의 공역체계 내 운용에 관한 연구)

  • Kim, Do-Hyun;Kim, Joong-Wook
    • Journal of the Korean Society for Aviation and Aeronautics
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    • v.19 no.1
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    • pp.101-107
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    • 2011
  • A UAV is defined as a powered, aerial vehicle that does not carry a human operator, and can fly autonomously or be piloted remotely. UAV operations have increased dramatically during the past several years in both the public and private sectors. The utilization of UAV and the activities of diverse widening, now the challenge was how to operate and integrate UAV safely in the NAS. The purpose of this study is to look around the trend for operability of Smart UAV in the NAS and to provide its implications and the future direction of integrated operating airspace focusing on U.S. where R&D and demand of UAV are the most in the world.

Effect of Chain Type Mole-Drainer Attached to Tractor for Drainage Improvement (배수개선을 위한 트랙터 부착식 체인형 암거 천공기의 적용 효과)

  • Jeon, Jonggil;Choi, Yonghun;Kim, Minyoung;Kim, Youngjin;Lee, Sangbong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.62 no.4
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    • pp.63-73
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    • 2020
  • The area of paddy fields for upland crop cultivation is gradually increasing. In this context, we developed a chain type mole drainer that can be attached to a tractor to prevent the wet injury of crops. To conduct a field test, we formed underdrains at 2 m intervals in a paddy field wherein soybeans are cultivated, and we investigated the status of the soil moisture, groundwater level, and soybean growth during the cultivation period. The soil moisture content decreased by 22.3~26.8% in the test plot and by 5.9~6.9% in the control plot during a period of 57~88 hour after a rainfall of 41.5~157.0 mm. The effect of underdrainage was clear as the hourly groundwater level decreased approximately 2.8 times faster in the test plot compared with the control plot. Moreover, the soybean yield was greater by 78.6 kg/10a in the test plot than in the control plot. Therefore, for soybean cultivation in paddy fields, the use of the developed mole drainer is expected to help in improving the drainage and increasing the soybean yield.

Development of a Long-slope Water Harvesting System in Natural Channel for Drought Mitigation in Upland (밭작물 가뭄피해 경감을 위한 소류천 유출수 저수 시스템 개발)

  • Kim, Youngjin;Choi, Yonghun;Lee, Sangbong;Kim, Minyoung;Jeon, Jonggil
    • Journal of The Korean Society of Agricultural Engineers
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    • v.62 no.6
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    • pp.111-118
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    • 2020
  • This study developed a rainwater harvesting system for the irrigation of upland on sloping area. The assessment of water supply capacity was evaluated in farm field experience. This system consists of a water catchment device and a collapsible storage tank. The water catchment device was designed to collect runoff water in natural channel of 500 mm width into a pipe of 50 mm inner diameter. The device has funnel-shaped plan and cross-section of square. The storage capacity of the collapsible water tank was caculated to meet the water demand for irrigation in 30 a cultivated land for 10-year frequancy drought. The tank has a cuboid shape with a capacity of 30 ㎥, 5 m in width and length, 1.2 m in height. This system can supply 92% of the water required for drop irrigation of red pepper and 88% of the water required for drop irrigation of onions in 30 a cultivation land during the month of May and June. In the case of 16-dry days of 10-years frequency, this system is capable to irrigate 100% of required water for red pepper and onion, 76.7% of required water for Omija (Schisandra chinensis), and 51.5% of required water for autumn kimchi cabbage.