• 약학회지
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• 제45권2호
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• pp.214-219
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• 2001

Nineteen hydrolysable tannins isolated from the Euphorbia helioscopia (Euphorbiaceae) were tested for the inhibitory effect on mushroom tyrosinase activity in vitro. Inhibitory effect of gallotannin group exhibited more potent than that of phenolcarboxylic acid and ellagitannin groups against the enzyme activity. The inhibitory activity by pentagalloyl glucose on mushroom tyrosinase was more potent ($IC_{50}$/, 4.9 $\mu$M) than that of kojic acid ($IC_{50}$/, 8.7 $\mu$M).

• 대한화장품학회지
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• 제23권3호
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• pp.115-121
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• 1997

To identify inhibitors of melanogenesis, we compared the effects of 5 compounds on mushroom tyrosinase, human melanocytic tyrosinase activity and melanin content. The cytotoxicyty of the components were also tested on cultured human melanoctes. Kojic acid showed marked inhibitory effect both on mushroom and human tyrosinase activity. This action of kijic acid is stronger than that of ascorbic acid. Arbutin inhibited human tyrosinase activity of cultured melanocytes although it had slightly inhibitory effect on mushroom tyrosinase activity. Azelaic acid had no effect on human tyrosinase activity. Melanin production was inhibited significantly by kojic acid and tranexamic acid. MTT assay showed that all of the compounds were non-cytotoxic to melanocytes at the concentrations tested. These results suggest that the effect of kojic acid on cultured meanocytes involve inhibition of tyrosinase activity and melanogenesis without affection the cell number.

• 생명과학회지
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• 제16권3호
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• pp.396-399
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• 2006

고구마(품종: 생미)추출액에 tyrosinase의 활성을 저해하는 저해 물질이 존재함을 확인하였으며, 고구마 추출물의 첨가농도를 증가시킬수록 tyrosinase 활성의 저해도가 증가하였다. 그리고 고구마 추출물에 존재하는 tyrosinase 저해제는 $95^{\circ}C$에서 20분간 열처리에 의하여 37.3%의 활성이 잔존하여 열에 비교적 안정하였으며, pH 5.0-7.0에서 가장 안정하였다. 그리고 고구마 추출물은 투석에 의하여 tyrosinase에 대한 저해활성이 유의적으로 감소하였으며, 따라서 고구마 추출물에 존재하는 tyrosinase의 저해활성은 저분자 물질로 추정된다.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1977-1983
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• 2006

Kaempferol was isolated and identified from the methanol extract of the flowers of Impatiens balsamina. Kaempferol showed inhibitory activity against mushroom tyrosinase with an $ID_{50}$ of 0.042 mM. Inhibition kinetics, as determined using a Lineweaver-Burk plot, showed kaempferol to be a competitive inhibitor of mushroom tyrosinase with a $K_i$ value of 0.011 mM. The lag phase of tyrosine hydroxylation catalyzed by mushroom tyrosinase clearly increased on increasing the concentration of kaempferol. In addition to its tyrosinase inhibiting activity, kaempferol strongly inhibited melanin production by Streptomyces bikiniensis, in a dose-dependent manner, without inhibiting cell growth. For comparative purposes, the tyrosinase inhibitory activity of kaempferol was also assayed versus quercetin, a positive standard.

• 약학회지
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• 제45권5호
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• pp.522-528
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• 2001

Tyrosinase plays an important role in the process of melanin biosynthesis, and it is a biochemical target enzyme for skin-whitening agents and the remedy for disturbances in pigmentation. We have screened 25 natural plant extracts for inhibitory effects against DOPA oxidase activity of tyrosinase. For the inhibitory effect against DOPA oxidase activity of tyrosinase, the recombinant human tyrosinase and the purified mushroom tyrosinase were used. Each of the dried plants extracted with methanol, and then the extracts were subjected to sequential fractionations with methylene chloride, ethyl acetate, n-butanol and water, respective The methylene chloride fractions of Angelica tenuissima, Nardostachys chinensis, Bombyx mop and Saposhnikovia divaricata, and the n-butanol fraction of Bombyx mori notably inhibited the human tyrosinase activity as well as mushroom tyrosinase activity (more than 90% inhibition). This study suggests that the above extracts have a potential as whitening agents as single ingredients or in combination with other of the extracts.

• 생약학회지
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• 제35권4호통권139호
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• pp.380-383
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• 2004

The purpose of this study was to evaluate mushroom Tyrosinase inhibitory activity of Cuscuta japonica Choisy and C. australis R.Be. The experimental materials were expressed juice from their stems and flowers, both water and ethanol extracts, their seeds, and two kinds of commercially available cosmetic packing Wontosa and Bupjetosa (made from seeds of C. japonica). The 50% inhibitory concentration $(IC_{50})$ of C. Japonica juice was 5.4 mg/ml. However, C. australis juice showed negligible mushroom tyrosinase inhibitory activity. The $IC_{50}$ of water extracted C. japonica seed was $54.0\;{\mu}g/ml$, water extracted product of Wontosa $50.0\;{\mu}g/ml$ and Bupjetosa $40\;{\mu}g/ml$. The $IC_{50}$ of ethanol extracted C. japonica seed was $10\;{\mu}g/ml$, Wontosa $10\;{\mu}g/ml$ and Bupjetosa $20\;{\mu}g/ml$.

• 대한한방피부미용학회지
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• 제1권1호
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• pp.91-97
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• 2005

(1) Objectives: To discover natural skin-lightening agents, we have evaluated the inhibitory activity of EtOH extracts from 20 medicinal plants against mushroom tyrosinase. (2) Methods: Tyrosinase activity was determined by the dopachrome method using L-tyrosine as the substrates. (3) Results: Of the plant extracts tested, the extracts of 4 plants, Albizzia julibrissin, Curcuma longa, Anethum graveolens and Sophora flavescens, exhibited potent inhibitory activity (> 50%) in mushroom tyrosinase assay. Four plant extract, extracts of Agrimonia pilosa, Paeonia moutan, Magnolia obovata and Eugenia caryophyllata also showed relatively strong inhibitory (> 40%) against mushroom tyrosinase. (4) Conclusion: These active medicinal plants may be useful for the development of skin-whitening agents. Since the active medicinal plants may contain effective tyrosinase inhibitors even more than kojic acid, further study to identify the active constituents from the plants is expected.

• 한국식품영양과학회지
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• 제46권3호
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• pp.320-326
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• 2017

본 연구에서는 낙과 배 물 추출물에 존재하는 미백기능 유효성분으로써 arbutin을 선정하여 HPLC를 통해 가장 높은 함량을 보인 낙과 배 물 추출물로 미백 효과를 알아보았다. 낙과 배 물 추출물의 미백 효과를 알아보기 위하여 B16F10 melanoma 세포를 이용하여 세포 내 tyrosinase 저해 효과, melanin 생성 저해 효과, tyrosinase와 관련된 단백질 발현에 미치는 영향을 연구하였다. 그 결과 낙과 배 물 추출물은 tyrosinase 활성과 B16F10 melanoma 세포의 melanin 생성 저해 효과를 나타내었다. 또한, MiTF와 tyrosinase의 발현의 억제를 통해 미백 효과를 나타내는 것으로 확인되었으며, mushroom tyrosinase 결과를 통해 melanin 생성 과정에 있어 tyrosinase를 직접 저해하는 것이 아닌 세포 내에서 tyrosinase의 발현 조절을 통해 저해하는 것으로 확인하였다. 따라서 낙과 배 물 추출물은 천연 arbutin 미백 기능성 화장품 소재 개발로 이용 가능성이 높은 것으로 판단된다. 낙과 배를 이용한 새로운 뷰티케어 소재를 개발하고 이들의 피부 기능성을 밝혀냄으로써 낙과 배의 다양한 활용성이 증대될 것으로 기대되며, 이를 통하여 국내 과실 농가 및 관련 사업에 기여할 것으로 생각된다.

• Journal of Pharmaceutical Investigation
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• 제36권2호
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• pp.103-107
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• 2006

In order to develop hydroquinone analogues for topical delivery, a structure-activity relationship study has been performed. A series of 2-substituted hydroquinones were tested for their ability to inhibit mushroom tyrosinase, alter melanin release and exert cytotoxicity in B6-F10 melanocytes. The electronic property of the 2-substituents did not affect the tyrosinase inhibition nor melanocyte toxicity. However, lipophilicity did affect to some degree the tyrosinase inhibition. The discrepancy in the structure-activity relationship may be due to the poor aqueous solubility of select analogues. Compounds with steric bulk at the 2-position seems to be less soluble, not enabling the analogue to interact effectively with the tyrosinase enzyme. Among the analogues tested, 2-isopropyl hydroquinone seems to be the most promising candidate for topical delivery, being the least toxic analogue with moderate melanin release inhibition.

• Preventive Nutrition and Food Science
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• 제6권1호
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• pp.16-18
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• 2001

The inhibitory effect of onion extract on mushroon tyrosinase activity was investigated. The enzyme activity was inhibited by 96.3% with th addition of onion extract. The inhibitor action of onion extract toward mushroom tyrosinase activity slightly increased after heat treatment at 10$0^{\circ}C$ for 10 mn (97.5%) and decreased after incubation of the extract at pH 2.3 for 3hrs (79.9%). However, the inhibitor action of the extract after dialysis decreased to18.8%. The onion extract showed drastic inhibition of the browning in mushroom.