• Title/Summary/Keyword: muscle stage larva

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Expressed Sequence Tags of Trichinella spiralis Muscle Stage Larvae

  • Park, Hae-Kyung;Chang, Seong-Won;Kang, Se-Won;Cho, Min-Kyoung;Choi, Sun-Hee;Hong, Yeon-Chul;Lee, Yong-Seok;Jeong, Hae-Jin;Yu, Hak-Sun
    • Parasites, Hosts and Diseases
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    • v.46 no.2
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    • pp.59-63
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    • 2008
  • In order to obtain greater insight into the relevant genomic expression patterns of Trichinella spiralis, 992 expressed sequence tags (ESTs) were collected from a cDNA library of T. spiralis muscle stage larvae and assembled into 60 clusters and 385 singletons. Of them, 445 (44.7%) ESTs were annotated to their homologous genes, and small fractions were matched to known genes of nematodes. The annotated ESTs were classified into 25 eukaryotic orthologous groups (KOG). Cytochrome C oxidase (34 clones) was found to be most frequent species.

Survey of Exotic Ox-warble Fly, Hypoderma bovis at the High Mountain Region in Korea (고산지(高山地)에 정착(定着)한 쇠가죽파리, Hypoderma sp.에 관한 연구(硏究))

  • Jang, Du-hwan;Han, Hong-ryul;Chung, Jae-young
    • Korean Journal of Veterinary Research
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    • v.27 no.2
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    • pp.301-306
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    • 1987
  • The exotic ox-warble fly, Hypoderma bovis has been established its life cycle at the high mountain region on the middle part of Korea. Milk cattle and Korean cattle of S pasture, H pasture and farms were infested with the third stage larva of the flies. Infection rates were 54.5% in S pasture and 30.4% in H pasture or farms. The second or third stage larve were detected from the back side of cattle and the muscle around epidual fat at autopsy. The grubs of ox-warble fly were throughly identified as Hypoderma bovis by the characteristics of spines or tubercles on the segments and stiginal plates.

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Larval Gncthostoma nipponicum found in the imported Chinese loaches (중국산 수입 미꾸리에서 검출한 Gnahostoma nipponicum 유충의 형태)

  • Son, Un-Mok;Go, Won-Gyu;Lee, Sun-Hyeong
    • Parasites, Hosts and Diseases
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    • v.31 no.4
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    • pp.347-352
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    • 1993
  • Six early third-stage larvae of Gnnthostoma nipponicum were recovered from the muscle of 376 loaches, Misgurnw anguiILicnudatus, imported from China. They were 614 × 114㎛ in average size, almost colorless except brownish intestine, and encircled by about 229 transverse rows of minute cuticular spines. Their head bulbs provided with 3 rows of hooklets, of which average number were 34.5 on the first, 36.7 on the second and 39.7 on the third. According1y, it is revealed that the Chinese loach is a natural second intermediate host of G. nipponicum and G. nipponicum has been distributed somewhere in China. This parasite may infect human when the imported loaches are consumed raw.

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Larval Gnathostoma spinigerum Detected in Asian Swamp Eels, Monopterus albus, Purchased from a Local Market in Yangon, Myanmar

  • Chai, Jong-Yil;Sohn, Woon-Mok;Na, Byoung-Kuk;Park, Jong-Bok;Jeoung, Hoo-Gn;Hoang, Eui-Hyug;Htoon, Thi Thi;Tin, Htay Htay
    • Parasites, Hosts and Diseases
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    • v.53 no.5
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    • pp.619-625
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    • 2015
  • The present study was performed to determine the infection status of swamp eels with Gnathostoma sp. larvae in Myanmar. We purchased total 37 Asian swamp eels, Monopterus albus, from a local market in Yangon in June and December 2013 and 2014. All collected eels were transferred with ice to our laboratory and each of them was examined by the artificial digestion technique. A total of 401 larval gnathostomes (1-96 larvae/eel) were detected in 33 (89.2%) swamp eels. Most of the larvae (n=383; 95.5%) were found in the muscle. The remaining 18 larvae were detected in the viscera. The advanced third-stage larvae ($AdL_3$) were 2.3-4.4 mm long and 0.25-0.425 mm wide. The characteristic head bulb ($0.093{\times}0.221mm$ in average size) with 4 rows of hooklets, muscular long esophagus (1.025 mm), and 2 pairs of cervical sacs (0.574 mm) were observed by light microscopy. The average number of hooklets in the 1st, 2nd, 3rd, and 4th rows was 41, 45, 48, and 51, respectively. As scanning electron microscopic findings, the characteristic 4-5 rows of hooklets on the head bulb, a cervical papilla, tegumental spines regularly arranged in the transverse striations, and an anus were well observed. Based on these morphological characters, they were identified as the AdL3 of Gnathostoma spinigerum. By the present study, it has been confirmed for the first time that Asian swamp eels, M. albus, from Yangon, Myanmar are heavily infected with G. spinigerum larvae.

Experimental Studied on the Second Intermediate HoBts of Clonorchis sinensis IV. Observations on the fate of metacercariae of Clonorchis sinensie in the fish hast, CuffricuEus eigenmunni (간흡충 제이중간숙주에 관한 실험적 연구 IV. 치리체내에 있어서 간흡충 피낭유충의 운명)

  • Lee, Jae-Gu;Baek, Byeong-Geol;Lee, Ho-Il
    • Parasites, Hosts and Diseases
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    • v.23 no.1
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    • pp.79-86
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    • 1985
  • In order to judge appropriately the suitability as the second intermediate host of Clonorchis sinensis, the present experiments were proposed to survey the metacercaria of C. sinensis in the fish host, Cuztriculus eigenmanni, in the 6eld, and to observe, by experimental infection of the fish with Clonorchis cercaria, the penetrating ability of the cercaria, maturity, the process of degeneration and extinction, and infectivity of the metacercaria. The following is a brie summary of the leading facts gained through the experiments. 1. All the ten fish (mean body weight $13.4{\pm}3.439$) which were caught in Ok-ku irrigation reservoir were in acted with the metacercariae and the average number of the metacercariae found in the fish body/gram was 4.55. 2. The Bish was easily subject to invasion of the cercaria, and the examination of the cercariae after having the fish infected disclosed that 5:l hours after the infection, all of the cercariae formed their cysts in muscle and the metacercariae kept growing, that 7 days later the metacercariae were found folding their bodies twice, and that in 15 days the characteristic structure of the metacercariae was complete and made a vigorous rotary movement intermittently. 3. In the case of control fish, Pseudorasbora parka, both penetrating ability of the cercariae and the infective degree of the metacercariae to control host were stronger as compared with the 5sh host because that the control fish had higher suceptibility to C. sinensis. 4. Afterwards, the metacercariae in the fish host came to a state of maturity and beyond this stage some metacercariae started the Process of degeneration and extinction in as early as 30 days, and as more days elapsed, mortality of the metacercariae was not uniformly increased but difference of mortality appeared to be according to the individual of Ssh host. That is, in 390, 540 and 586 days, all of the metacercariae in the fish hosts became dead, meanwhile almost all of those found in the fish host of 596 days after the infection survived (mortality; 8, 51%) , Percentage of orally administered metacercariae developing to maturity in rabbit is 10.465 in the last case. 5. As the days were progressed the shapes of the metacercariae in the fish host tended to change from ellipsoid to almost round shapes, the length became shorter while the width was wider. The results shown above revealed that C. eigenmanni was, though it could not be proper host than p. larva. suitable as the second intermediate host in addition to our previous observation results.

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