• 동의생리병리학회지
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• 제23권4호
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• pp.825-830
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• 2009

The purpose of this research was to investigate the effects of Calendula officinalis extract (CE) on the activation of murine lymphocytes and macrophages. CE was administered p.o. once a day for 7 days at the concentration of 500 mg/kg. The administration of CE increased the viability of thymocytes, but decreased the viability of splenocytes. Also, CE increased the viability of thymocytes and splenocytes at the concentration of $5{\mu}g$/ml in vitro. The administration of CE did not affect the population of thymic $CD4^+$/$CD8^+$ cells and splenic $CD4^+$/$CD8^+$ cells. Furthermore, the administration of CE enhanced the phagocytic activity of peritoneal macrophages, but decreased the phagocytic activity in vitro. CE decreased the production of nitric oxide from peritoneal macrophages in vivo and in vitro system. These results suggest that CE enhance of cell viability by a direct influence on thymocytes and phagocytic activity by an indirect influence on peritoneal macrophages.

• 동의생리병리학회지
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• 제25권5호
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• pp.837-842
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• 2011

The purpose of this research was to investigate the effects of 50% ethylalcohol extracts of Lespedeza cuneata G. Don (LE) on the activity of murine immune cells. LE was administered orally once a day for 7 days at the dose of 250 mg/kg. LE increased the cell viability of thymocytes, splenocytes and peritoneal macrophages in vitro and in vivo system, but decreased the cell viability of thymocytes and splenocytes in the presence of concanavalin A in vivo system. Also, the administration of LE was increased the production of ${\gamma}$-interferone, but did not affect the production of interleukin-2 and interleukin-4. Furthermore, LE decreased the phagocytic activity of peritoneal macrophages in vitro and in vivo system, but enhanced the production of nitric oxide. These results suggest that LE has a immuno-regulative action via stimulation of immune cells proliferation.

• Journal of Ginseng Research
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• 제10권2호
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• pp.133-140
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• 1986

면역 작용에 중요한 역할을 하는 림프구의 일종인 T세포가 성장 및 증식하는 기관인 흉선의 흉선세포증식과 생존력에 미치는 인삼 saponin의 영향과, 흉선 세포의 증식에 관여하는 Interleukin2(IL2)에 미치는 인삼saponin의 영향과,IL2의 생성에 영향을 미치는 Adenosin Deaminase(ADA)에 대한 인삼 saponin의 영향 등을 알아보기 위하여 생쥐의 흉선세포를 사용하여 다음과 같은 결과를 얻었다. 여러 농도의 인삼 saponin에서 배양한 전체 흉선세포수와 살아있는 흉선세포수의 변화는 $10^{-5}$%농도에서 대조군보다 증가하였으나 $10^{-1}$%농도군에서는 감소되었다. 여러 농도의 IL2에 인삼 saponin$10^{-5}$%농도를 첨가하여 배양한 전체 흉선세포수는 1.5%,3%및 6%농도군에서 9시간 또는 12시간배양시 증가하였으며,6% 실험군은 24시간과 48시간 배양시에도 유의성있게 증가하였다. 그러나 살아있는 흉선세포수는 대조군과 별차이가 없었다. 마지막으로 흉선세포의 ADA활성도에 미치는 인삼 saponin의 영향은 $10^{-5}$%농도에서 25%의 유의성있는 활성 증가가 일어났다.

• 한국식품영양학회지
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• 제14권2호
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• pp.99-105
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• 2001

Immunoregulative action of 70% ethyl alcohol fraction of Cervus nippon was investigated in vitro. The fraction enhanced the proliferation of thymocytes and the population of CD4$\^$+/CD8$\^$-/ single-positive cells in thymocytes. CN-E enhanced [Ca$\^$2+]$\sub$i/, the production of TNF-${\alpha}$ and IL-1 ${\beta}$, and phagocytosis in peritoneal macrophages. The fraction did not induce the proliferation of splenocytes and DNA fragmentation in thymocytes. These results indicate that 70% ethyl alcohol fraction of Cervus nippon regulates immunological action by stimulating murine thymocytes and macrophages.

• Archives of Pharmacal Research
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• 제21권3호
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• pp.266-272
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• 1998

The present study investigated the antitumor activity of the aqueous-alcoholic extracts from unripe cotton balls of Gossypium indicum. An Exposure of murine B16 melanoma and L1210 lymphoma cells to the extracts resulted in their severe deaths in time- and concentration-dependent manners. Of the extracts, hydrophilic fractions were most efficacious for the antitumor activity and found to contain certain amounts of catechin and its derivatives. The hydrophilic extract fraction C36B2-8 had approximately 10 times more cytotoxic effects on B12 and L1210 cells than on isolated murine thymocytes. High concentrations (>150 $\mu$g/ml) of C 36B3-8 mainly induced necrotic cell death. At low concentrations (<100 $\mu$g/ml), however, C 36B3-8 induced not only necrosis but also apoptosis of the two tumor cell lines, which was proved by the TUNEL staining and DNA fragmentation techniques. The data indicate that certain ingredients of the cotton ball extract of G. indicum have an antitumor activity.

• 동의생리병리학회지
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• 제17권2호
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• pp.481-485
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• 2003

Experimental studies were done to research the clinical effects of Juglandis Semen aqua-acupuncture and acupuncture(BL13, BL17, BL13ㆍ17 and free points) on the anti-allergic inflammation response. The following results have been obtained ; JSAA was increased in the lymphocyte proliferation in cultured murine thymocytes and splenocytes. The production of IL-1β was decreased in Juglandis Semen aqua-acupuncture and acupuncture(BL13, BL17 and BL13ㆍ17) group. The production of IL-4 was decreased in Juglandis Semen aqua-acupuncture and acupuncture(BL13, BL17 and BL13ㆍ17) group. The production of total IgG was decreased in Juglandis Semen aqua-acupuncture(BL13 and BL13ㆍ17) and acupuncture(BL13, BL17 and BL13ㆍ17) group. According to the above results, Semen aqua-acupuncture and acupuncture(BL13, BL17 and BL13ㆍ17) both enhances the specific immune response in T and B cells and depress the allergic inflammation reaction.

• 동의생리병리학회지
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• 제19권3호
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• pp.705-709
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• 2005

The purpose of this research was to investigate the effects of Coix Lachryma-Jobi L. var. Ma-yuen Stapf. on the proliferation of L1210 cells and immune cells. The hexane fraction of Coix Lachryma-Jobi decreased the proliferation of L1210 cells and induced DNA fragmentation of L1210 cells. Also, the fraction decreased the cell viability of murine thymocytes and splenocytes, but increased the phagocytic activity of murine peritoneal macrophages. In addition, fatty acids and fatty acid methyl esters decreased the proliferation of L1210 cells and induced DNA fragmentation of L1210 cells. The main components of the fraction are fatty acid and their derivatives. These results indicate that Coix Lachryma-Jobi has a cytotoxicity on tumor cells and immune cells.

• 동의생리병리학회지
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• 제21권5호
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• pp.1219-1225
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• 2007

Immunological activities of Ginseng Radix water extract (GE) were compared with theirs of Insamhodo-tang water extract (IHT). GE and IHT (500 mg/kg) were administered p.o. twice a day for 5 days to C57BL/6 mice, respectively. IHT decreased the cell viability of murine thymocytes and splenocytes, but increased the population of $CD4^+$ cells in thymocytes, and the population of $Thy1^+$ cells and $CD4^+$ cells in splenocytes in contrast to GE. In addition, IHT decreased the phagocytic activity of peritoneal macrophages, but increased the production of nitric oxide from peritoneal macrophages in contrast to GE. These results suggest that IHT decrease a specific- and nonspecific immune reaction in comparison with GE.

• 약학회지
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• 제46권1호
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• pp.69-74
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• 2002

The effects of genistein on murine thymocytes for inducing apoptotic cell death and phagocytic activity of peritoneal macrophage were studied in vitro. Addition of genistein (10 and 50$\mu$M) to cultured thymocytes from BALB/c mice definitely promoted DNA fragmentation. Also, cytofluorometric analysis of these cells demonstrated a reduction in mitochondrial transmembrane potential ($\Delta$Ψm). But, repeated administration of genistein (1 mg/mouse/day) to mice for 7 days did not cause any detectable DNA fragmentation. Genistein decreased lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particles in peritoneal macrophage. These results suggest that genistein induce an apoptosis of thymocyte via reduction in $\Delta$Ψm and decrease phagocytic activity of peritoneal macrophage in vitro.

• 사상체질의학회지
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• 제12권1호
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• pp.186-200
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• 2000

소양인(少陽人) 인동등지골피탕(忍冬藤地骨皮湯)이 중소(中消) 합병증(合倂症)에 미치는 영향(影響)을 살펴보고자 하였고 결과(結果)는 다음과 같다. 1. 장관운동(腸管運動)을 억제(抑制)하였다. 2. Acetic acid에 의한 writhing syndrome을 억제(抑制)하였다. 3. Evans blue에 의한 모세혈관투과성(毛細血管透過性)을 억제(抑制)하였다. 4. Histamine에 의한 급성족부종(急性足浮腫)을 억제(抑制)하였다. 5. Cotton pellet에 의한 육아종(肉芽腫) 형성(形成)을 억제(抑制)하였다. 6. 흉선세포(胸線細胞) 및 비장세포(脾臟細胞)의 세포생존율(細胞生存率)을 증가(增加)시켰다. 7. 흉선세포(胸線細胞)로부터 ${\gamma}-IFN$ 및 IL-2의 분비(分泌)를 억제(抑制)하였다. 8. 비장세포(脾臟細胞)로부터 IL-4의 분비(分泌)는 증가(增加)시켰으나, $TNF-{\alpha}$ 및 $IL-1{\beta}$의 분비(分泌)는 억제(抑制)하였다. 9. 복강(腹腔) 대식세포(大食細胞)로부터 $TNF-{\alpha}$, $IL-1{\beta}$ 및 nitric oxide의 분비(分泌)를 억제(抑制)하였다. 10. 복강(腹腔) 대식세포(大食細胞)의 lucigenin chemiluminescence 및 FITC-conjugated E. cole engulfment를 촉진(促進)하였다. 이상의 실험(實驗) 결과(結果) IJTE는 장관운동(腸管運動)을 억제(抑制)하였으며, 진통(鎭痛) 및 항염작용(抗炎作用)을 나타냈고, 이들 작용은 면역세포(免疫細胞)로부터 $TNF-{\alpha}$, $IL-1{\beta}$ 및 nitric oxide의 생성(生成)을 억제(抑制)하기 때문인 것으로 추정된다. 따라서 이러한 기전(機轉)에 의하여 인동등지골피탕(忍冬藤地骨皮湯)은 중소(中消) 합병증(合倂症)의 하나인 염증(炎症)을 억제(抑制)하는데 유효(有效)한 것으로 사료(思料)된다.