Browse > Article

Effect of Genistein on Apoptosis of T Iymphocyte and Phagocytosis of Peritoneal Macrophage  

은재순 (우석대학교 약학대학)
조선경 (우석대학교 약학대학)
이택렬 (우석대학교 약학대학)
김대근 (우석대학교 약학대학)
오찬호 (우석대학교 생명공학부)
소준노 (우석대학교 생명공학부)
Publication Information
YAKHAK HOEJI / v.46, no.1, 2002 , pp. 69-74 More about this Journal
Abstract
The effects of genistein on murine thymocytes for inducing apoptotic cell death and phagocytic activity of peritoneal macrophage were studied in vitro. Addition of genistein (10 and 50$\mu$M) to cultured thymocytes from BALB/c mice definitely promoted DNA fragmentation. Also, cytofluorometric analysis of these cells demonstrated a reduction in mitochondrial transmembrane potential ($\Delta$Ψm). But, repeated administration of genistein (1 mg/mouse/day) to mice for 7 days did not cause any detectable DNA fragmentation. Genistein decreased lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particles in peritoneal macrophage. These results suggest that genistein induce an apoptosis of thymocyte via reduction in $\Delta$Ψm and decrease phagocytic activity of peritoneal macrophage in vitro.
Keywords
genistein; thymocyte; DNA fragmentation; mitochondrial transmembrane potential; macrophage; phagocytosis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Individual and combinaory effects of soy isoflavones on the in vitro potentiation of lymphocyte activation /
[ Wang, W.;Higuchi, C. M.;Zhang, R. ] / Nutr. Cancer.   DOI   ScienceOn
2 Rapid and Simple method for mesuring thymocyte apoptosis by propidium iodide staining and flow cytometry /
[ Nicoletti, I.;Migliorati, G.;Pagliacci, M. C.;Grignani, F.;Riccardi, C. A. ] / J. Immuno. methods   DOI   ScienceOn
3 Interactions between the gonadal steroids and the immune system /
[ Grossman, C. J. ] / Science   DOI
4 Phytoestrogens; epidermiology and a possible role in cancer protection /
[ Adlercreurz, H. ] / Environ.Health Perspect
5 Evidence of synergy between Thy-1 and CD3/TCR complex in signal deluvery to murine thymocytes for cell death /
[ Nakashima, I.;Zhang, Y. H.;Rahman, S. M. J.;Yoshida, T.;Isobe, K.;Ding, L.;Iwamoto, T.;Hamaguchi, M.;Ikezawa, H.;Taguchi, R. ] / J. Immunology.
6 Soybeans inhibit mammary tumors in models of breast cancer /
[ Barnes, S.;Grubbs, C.;Setchell, KDR.;Carlson, J. ] / Prog. Clin. Biol. Res.
7 Enhancement of immune function in mice fed high doses of daidzein /
[ Zhang, R.;Li. Y.;Wang, W. ] / Nutr. Cancer   DOI   ScienceOn
8 Induction of mouse thymocyte apoptosis by inhibitors of tyrosine kinases is associated with dephosphorylation of nuclear proteins /
[ Azuma, Y.;Onishi, Y.;Sato Y.;Kizaki, H. ] / Cell Immuno   DOI   ScienceOn
9 Intra- and extracellular evevts in lumino-dependent chemilulinescence of polymorphonuclear leukocytes /
[ Breiheim, G.;Stendahl, O.;Dahlgren, C. ] / Infect. Immun
10 Nonsteroidal estrogens of dietary origin; Possible roles in hormone-dependent disease /
[ Setchell, K. D. R.;Borriello, S. P.;Hulme, P. ;Kirk, D. N.;Axelson, M. ] / Am. J. Clin. Nutr.   DOI
11 Effect of isoflavones genistein and daidzein in the inhibition of lung metastasis in mice induced by B16F-10 melanoma cells /
[ Meno, L. G.;Muttan, R.;Nair, M. G.;Chang, Y. G.;Kutten, G. ] / Nutrition and Cancer   DOI   ScienceOn
12 A rapid and simple microfluotometric phagocytosis assay /
[ Chok, P. W.;Choon, S. P.;Benjamin, H. S.;Hating, M. J. G. ] / J. Immunol. Methods   DOI   ScienceOn
13 The role of soy products in reducing risk of cancer /
[ Messina, M.;Barnes, S. ] / JNCI   DOI
14 Rapid colorimetric assay for celluar growth and survival: application to proliferation and cytotoxic assays /
[ Mosmann, T. ] / J. Immunol. methods
15 Characterization of lymphocyte-activating factor(LAF) produced by the macrophage cell line P388D1 /
[ Mizel, S. B.;Openheim, J. J.;Rosensteich, D. L. ] / J. Immuno.
16 Hormonal regulation of immune responses /
[ Paavonen, T. ] / Ann. Med.   DOI   ScienceOn
17 Reduction in mitochondrial potential constitues an early irreversible stp of programmed lymphocyte death in vivo /
[ Zamzini, N.;Petit, P. X.;kroemer, G. ] / J. Exp. Med.   DOI   ScienceOn
18 Effect of soy-derived isoflavonoids on the induced growth of MCF-7 cells by estrogenic enviromental chemicals /
[ Verna, S. P.;Goldin, B. R. ] / Nutrition and Cancer   DOI   ScienceOn
19 Inhibition of Murine bladder tumorigenesis by soy isoflavones via alterations in the cell cycle, apoptosis and angiogenesis /
[ Zhou, J. R.;Mukherjee, P.;Gugger, E. T.;Tanaka, T.;Blackburn, L.;Clinton, S. K. ] / Cancer Research
20 genistein induces apoptosis in immature human thymocytes by inhiniting topoisorr..erase-II /
[ McCabe, M. J. Jr.;Orrenius, S. ] / Biochem. Biophys. Res. Commun.   DOI   ScienceOn
21 Regulation of the immune system by sex steroids /
[ Grossman, C. J. ] / Endocr. Rev.   DOI   ScienceOn
22 Western diet and western disease; Some hormonal and biochemical mechanism and associations. Scand /
[ Adlercreurz, H. ] / J. Cin. Lab. Invest.
23 Effects of soybean isoflavones on cell growth and apoptosis of the human prostatic cancer cell line LNCaP /
[ Onozawa, M.;Fukuda, K.;Ohtani, M.;Akaza, H.;Sugimura, T.;Wakabayashi, K. ] / Jpn. J. Clin. Oncol.   DOI   ScienceOn
24 Chemiluminenscence and nitrite determinations by the MALU macrophage cell line /
[ Boudard, F.;Vallot, N.;Cabaner, C.;Bastide, M. ] / J. Immunol. Methods   DOI
25 Use and specificity of genistein as inhibitor of protein-tyrosine kinases /
[ Akiyama, T.;Ogawara, H. ] / Methods Enzymol   DOI
26 Measurement of phagocyte chemiluminenscence in a microtiter plate format /
[ Blair, A. L.;Cree, I. A.;Beck, J. S.;Hating, M. J. G. ] / J. Immunol. Methods
27 The role of the immune system in the pathogenesis of cancer /
[ Appelbaum, J. W. ] / Semin. Oncol. Nurs.   DOI   ScienceOn
28 Soy intake and cancer risk;a review of in vitro and in vivo data /
[ Messina, M.;barnes, S. ] / Nutz Cancer
29 Effect of daidzein and genistein on immune function in mice /
[ Eun, J. S.;Cho, S. K.;Kwon, J.;Suh, E. S.;Jeon, H.;Yum, J. Y. ] / Yakhak Oheji   과학기술학회마을
30 Planning for lymphocytes: A method for cell selection /
[ Wysocki, L. J.;Sato, V. L. ] / Proc. Nat1. Acad. Sci. USA   DOI   ScienceOn
31 A simple and rapid method to determine hematopoietic growth factor activity /
[ Kotnic, V.;Fleischmann, W. R. Jr. ] / J. Immuno. methods   DOI   ScienceOn
32 Genistein, a specific inhibitor of tyrosine-specific protein kinase /
[ Akiyama, T.;Ishia, J.;Nakagawa, S.;Ogawara, H.;Watanabe, S. I. ] / J. Biol. Chem.