• Journal of Plant Biotechnology
• /
• 제3권3호
• /
• pp.141-144
• /
• 2001

To enhance the shoot induction efficiency from nodal stem of yooza, the culture conditions such as basal medium, carbohydrate source, solidifying agent and the optimum concentration of plant growth regulators for shoot induction were investigated. The nodal explants were cultured better on MS medium than on MT, SH, B5 or W media in considering of shoot induction rate and mean shoot length. Solidifying agent in medium was better with 0.8% agar than with 0.3% agar, 1.2% agarose or 0.2% gelrite. Carbohydrate source in shoot induction medium was efficient with 30 g/L sucrose. The optimum concentrations of plant growth regulators were determined that 0.1 mg/L NAA as auxin was effective on the shoot induction, and 1.0 mg/L BAP as cytokinin induced multiple shoots efficiently. Shoot induction was the most effective on MS medium supplemented with 4 mg/L $GA_3$ in yooza.

• Plant Resources
• /
• 제2권1호
• /
• pp.10-15
• /
• 1999

In vitro shoot regeneration from cotyledon and hypocotyl explants derived from germinating mature Impatiens seeds. The induction of organogenetic tissue was also influenced by the cotyledon and hypocotyl. Multiple shoot induction was higher in hypocotyl than in the cotyledon explant with Thidiazuron and a NAA medium.

• Plant Resources
• /
• 제8권3호
• /
• pp.286-292
• /
• 2005

Shoot induction system was developed in the recalcitrant plant species, Brassica rapa ssp. rapifera by using optimum selection of profit organ, phytohormone combination, seedling age and kind of culture container. Out of in vitro cultured leaf segment, petiole, hypocotyl, and cotyledon with petiole, only cotyledon with petiole derived from 4 day-old seedlings induced multiple shoot. The optimum combination of auxin and cytokinin for the multiple shoot induction was MS medium containing 5mg/L BA and 0.5mg/L NAA. The major factors for multiple shoot propagation were part of plant organ, age of seedling, and ratio of auxin and cytokinin. In addition, shoot regeneration was promoted in the 100ml Erlenmeyer flask compared with the $90mm{\times}20mm$ Petri-dish. The induced shoots formed roots easy on MS medium containing 0.1mg/L IBA and the whole plants were successfully cultivated in soil.

• Plant Biotechnology Reports
• /
• 제3권1호
• /
• pp.67-74
• /
• 2009

An efficient micropropagation protocol based on multiple shoot induction and callus regeneration has been standardized in Sarcostemma brevistigma, a rare medicinal plant. The nodal cuttings were cultured on MS medium supplemented with BA ($0.5-8{\mu}M$) or Kn ($0.5-8{\mu}M$) alone or in combination with NAA ($0.5-1.5{\mu}M$). Maximum multiple shoot induction was observed on MS medium supplemented with $4{\mu}M$ BA. On this medium, 100% cultures responded with an average number of 11.3 shoots per explant. However, the average shoot length was limited to only 0.9 cm on this medium. The addition of $1{\mu}M$ NAA along with $4{\mu}M$ BA gave rise to an average number of 10.9 shoots with an average shoot length of 1.8 cm. Luxuriantly growing callus was obtained on MS medium supplemented with BA ($5{\mu}M$) and 2,4-D ($2{\mu}M$). The callus was subcultured on MS medium supplemented with BA ($2-15{\mu}M$) or Kn ($2-15{\mu}M$) alone or in combination with NAA ($0.5-2{\mu}M$) for shoot organogenesis. Optimum callus regeneration was obtained on MS medium supplemented with $10{\mu}M$ BA and $1{\mu}M$ NAA. On this medium, 100% cultures responded with an average number of 13.4 shoots per culture. The shoots obtained via multiple shoot induction and organogenesis were rooted on half-strength MS medium supplemented with NAA ($1-7{\mu}M$) or IBA ($1-7{\mu}M$). IBA was better than NAA in terms of both the percentage of cultures that responded and the average number of roots per explant. The rooted shoots were successfully transplanted to soil with 86% success. This standardized protocol will help to conserve this rare medicinal plant.

• 식물조직배양학회지
• /
• 제21권4호
• /
• pp.247-252
• /
• 1994

조직배양을 통한 대량증식을 목적으로 구문초 (Pelalgonium citrosa)의 엽신, 엽병 및 정단분열조직을 auxin류와 cytokinin류의 생장조절제를 혼합첨가한 MS배지에 배양한 후 캘러스발생 및 shoot분화율을 조사하였고, 분화된 shoot는 NAA와 IAA가 단독첨가된 MS배지에 옮겨 뿌리분화율을 조사하였다. 생장조절제의 효과는 2,4-D와 kinetin의 혼용처리보다 NAA와 BA첨가배지에서 엽신, 엽병조직 모두 캘러스발생 및 multiple shoot분화가 양호하였다. 엽신조직의 경우 암배양 상태에서 캘러스와 shoot의 분화가 좋았고, 0.5 mg/L NAA와 BA 혼용배지에서는 정상적 민 shoot가 분화된 반면 NAA와 BA의 농도가 높을 경우 분화된 shoot는 vitrification현상이 비교적 많이 나타난다. Shoot는 캘러스를 통하거나 직접 절편체에서 분화되었는데 1개의 절편당 shoot분화수는 캘러스를 통해 분화된 경우가 훨씬 많았다. 엽보의 경우 캘러스발생 및 shoot분화양상은 엽신과 비슷하였으나 분화된 shoot수는 훨씬 적었다. 정단분열조직에서 캘러스증식율이 가장 좋았으며 shoot분화는 NAA와 BA 0.5mg/L첨가구에서 양호하여 대량증식에 가장 효과적인 반응이었다. Shoot로부터 뿌리발생은 1.0 mg/L NAA 첨가배지에서 가장 효과적이었다.

• Journal of Plant Biotechnology
• /
• 제5권2호
• /
• pp.107-113
• /
• 2003

The effect of sodium sulphate on shoot induction and multiple shoot formation from nodal explants of Vitex negundo L. was tested on Murashige and Skoog's (MS) medium fortified with different auxins, cytokinins and sucrose. Highest percentage $(97.78\%)$ of explants for shoot induction and multiple shoot (20.68/explant) production were observed in the combination treatment of $N^6-Benzyl$ adenine (BA) $(17.80\;{\mu}M/L)$, ${\alpha}-Naphthalene$ acetic acid (NAA) $(2.15\;{\mu}M/L)$ and $5\%$ sucrose supplemented with 100 mg/L sodium sulphate. In vitro raised shoots were rooted on the half-strength MS medium fortified with different concentrations of NAA, Indole-3-acetic acid (IAA), and Indole-3-butyric acid (IBA) alone and in combinations. Among the treatments, $4.90\;{\mu}M/L$ of IBA was found most effective $(95.56\%)$ in inducing roots. The rooted plantlets were shifted to glasshouse for acclimatization and later transferred to the field with cent percent survival. Furthermore, in vitro flowering was observed in the shoots cultured on MS medium supplemented with BA $(8.90\;{mu}M/L)$ and NAA $(1.61\;{\mu}M/L)$.

• Journal of Plant Biotechnology
• /
• 제1권2호
• /
• pp.79-83
• /
• 1999

A method for induction of multiple shoots using cotyledonary nodes and shoot tips of Macrotyloma uniflorum (Lam.) Verdc. was described. The experiment was conducted in which shoot induction was noticed on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of four cytokinins (KIN, 2iP, Ads, BAP). These multiple shoots were later developed into normal shoots. The highest rate of shoot proliferation came from MS medium added with BAP 1.5 mg/L. The multiple shoot buds were subcultured into MS medium with BAP (0.5-1.5 mg/L) along with Ads (1.0 mg/L) and GA$_3$ (0.5 mg/L), which gave rise to the highest frequency of shoot proliferation and elongation. The shoots were rooted on MS medium supplemented with 1.75 mg/L IBA.

• 아시안잔디학회지
• /
• 제8권3호
• /
• pp.137-147
• /
• 1994

We have established a high-frequency plant regeneration system via organogenesis from mature seed of Korean lawngrass(Zoysia japonica Steud.). The effects of 2,4-dichiorophenoxy acetic acid (2,4-D), 6-furfuryl amino purine (kinetin), $\alpha$-naphthaiene acetic acid (NAA), N6-benzyl amino pu-rine (BAP), and casein hydrolysate (CR) on cailus induction and multiple shoot formation on ex-posure to light were evaluated. Callus produced on the Murashige and Skoog (MS) medium containing 2,4-D and kinetin had high organogenesis potency. A single addition of 1.0 mg $L-^1$ 2,4-D significantly induced callus. Also, 1.0 mg L-$^1$ 2,4-D, with the addition of 0.1 mg $L-^1$ kinetin highly enlianced callus induction. The trend of cailus induction was also found on mediurn containing 0.1 mg $L-^1$ BAP with 1.0 mg $L-^1$2,4-D, and 1 g $L-^1$ CR with the addition of 1.0 mg $L-^1$ 2,4-D. However, NAA was no effective on callus formation. The growth of root was significantly high in the presence of 0.1 mg $L-^1$ kinetin compared to other concentrations. Over 2 mg $L-^1$ kinetin highly lengthened roots. Fresh weight of plantlet was highest on medium containing 0.1 mg $L-^1$ 2,4-D. Also, on medium containing 0.1 mg $L-^1$ BAP, fresh weight of piantlet was highly enhanced. BAP was significantly effective on multiple shoot formation, particularly when 2.0 mg $L-^1$ was added with 0.1 mg $L-^1$ 2,4-D. Callus induction and multiple shoot formation were achieved on MS basal medium containing 1.0 g $L-^1$ CH.

• Journal of Plant Biotechnology
• /
• 제29권1호
• /
• pp.51-57
• /
• 2002

대두의 재분화 체계를 확립하고자 품종별 shoot 형성률, 적정배지 및 적정조직을 구명하고자 하였다. 유묘의 조직부위별 비교에서는 1개의 자엽을 포함한 절에서 multiple shoot 형성률이 높았다. 기본배지에서는 1/2 B5배지가 효율적이었으며, TDZ 처리시에 multiple shoot 형성률이 높았으며, 처리농도는 2mg/L가 효율적이었다. Shoot 형성시 자엽 네 전분은 배양 1주까지 증가 후 감소하였으며, 수용성 당은 배양 2주까지 증가 후 shoot가 형성되는 배양 4주에는 관찰되지 않았다. 품종 간 비교에서는 품종에 따라 shoot 형성 양상이 구분되어, '흑청콩' 등 11품종에서는 multiple shoot 형성률이 높았고, '만리콩' 등 16품종에서는 pair shoot 형성률이 높았다. 자엽절에서 shoot가 형성되는 양상에 따른 조직화학적 관찰 결과 pair shoot의 경우 내생적으로 잠재한 액아에 의한 것이며, multiple shoot의 경우는 새로이 형성된 shoot로 확인되었다.

• Journal of Plant Biotechnology
• /
• 제6권1호
• /
• pp.39-43
• /
• 2004

A successful, efficient system for multiple shoot induction and plant regeneration of soybean (Glycine max) was established. Four soybean genotypes were compared for organogenic responses on various media cultured under light conditions. The adventitious shoots (98%, 2.6 shoots/cotyledon) directly from one-day-old cotyledon after germination induced by the hormone treatment and its efficiency was higher than any other conditions. The optimal medium for the induction of multiple shoots from cotyledon in Pungsannamulkong(shoot formation rate, 98%), Lx 16 (83%) and IIpumgeomjeongkong(63%) was MS medium supplemented with 2 mg/L BAP, but for Alchankong(75%), MS medium supplemented with 1mg/L zeatin and 1mg/L IAA, 3% sucrose, 4% Phytagel. Higher root induction (88%) was observed from the shoots placed on rooting medium (hormone-free MS basal). Plantlets were transferred onto the same medium supplemented with 1% activated charcoal for further development. With this treatment, regenerated plantlets were obtained within 7-8 weeks (shoot induction for 4 weeks, rooting and shoot elongation for 3-4 weeks).