Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Effect of Cytokinins on the Proliferation of Multiple Shoots in Horsegrgm [Macrotyloma uniflorum (Lam.) 'Verdc.]

  • Mohamed, Shamsudeen Varisai (Plant Tissue Culture Unit, Department of Planr Science, School of Life Sciences, Bharhthidasan University, India) ;
  • Jawahar, Manikam (Plant Tissue Culture Unit, Department of Planr Science, School of Life Sciences, Bharhthidasan University, India) ;
  • Thiruvengadam, Muthu (Plant Tissue Culture Unit, Department of Planr Science, School of Life Sciences, Bharhthidasan University, India) ;
  • Jeyakumar, Masilamani (Plant Tissue Culture Unit, Department of Planr Science, School of Life Sciences, Bharhthidasan University, India) ;
  • Jayabalan, Narayanasamy Pillai (Plant Tissue Culture Unit, Department of Planr Science, School of Life Sciences, Bharhthidasan University, India)
  • 발행 : 1999.01.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A method for induction of multiple shoots using cotyledonary nodes and shoot tips of Macrotyloma uniflorum (Lam.) Verdc. was described. The experiment was conducted in which shoot induction was noticed on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of four cytokinins (KIN, 2iP, Ads, BAP). These multiple shoots were later developed into normal shoots. The highest rate of shoot proliferation came from MS medium added with BAP 1.5 mg/L. The multiple shoot buds were subcultured into MS medium with BAP (0.5-1.5 mg/L) along with Ads (1.0 mg/L) and GA$_3$ (0.5 mg/L), which gave rise to the highest frequency of shoot proliferation and elongation. The shoots were rooted on MS medium supplemented with 1.75 mg/L IBA.

키워드