• Title/Summary/Keyword: multiple events

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Agrobacterium-Mediated Co-transformation of Multiple Genes in Metarhizium robertsii

  • Padilla-Guerrero, Israel Enrique;Bidochka, Michael J.
    • Mycobiology
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    • v.45 no.2
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    • pp.84-89
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    • 2017
  • Fungi of the Metarhizium genus are a very versatile model for understanding pathogenicity in insects and their symbiotic relationship with plants. To establish a co-transformation system for the transformation of multiple M. robertsii genes using Agrobacterium tumefaciens, we evaluated whether the antibiotic nourseothricin has the same marker selection efficiency as phosphinothricin using separate vectors. Subsequently, in the two vectors containing the nourseothricin and phosphinothricin resistance cassettes were inserted eGFP and mCherry expression cassettes, respectively. These new vectors were then introduced independently into A. tumefaciens and used to transform M. robertsii either in independent events or in one single co-transformation event using an equimolar mixture of A. tumefaciens cultures. The number of transformants obtained by co-transformation was similar to that obtained by the individual transformation events. This method provides an additional strategy for the simultaneous insertion of multiple genes into M. robertsii.

Mediating Effect of Mindfulness between Stressful Life Events and Depression in Nursing Students (간호대학생의 생활스트레스와 우울의 관계에서 마음챙김의 매개효과)

  • Song, Yeoungsuk
    • Journal of Korean Public Health Nursing
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    • v.31 no.3
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    • pp.518-527
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    • 2017
  • Purpose: This study examined the relationship among stressful life events, mindfulness and depression, and explored the mediating role of mindfulness between stressful life events and depression in nursing students. Methods: A cross-sectional survey was undertaken. A sample of 120 nursing students participated in the study. The self-reported measures of stressful life events (revised life stress scale), depression (DASS-D) and mindfulness (MAAS) were adopted in April, 2017. The data were analyzed using descriptive statistics, frequency, Pearson correlation coefficient and multiple regression. Mediation analysis was performed using the Baron & Kenny method and Sobel test. Results: The mean scores of stressful life events, mindfulness and depression were 39.50, 77.03, and 8.18, respectively. Higher levels of stressful life events were associated with lower mindfulness (r = -46, p < .001) and higher depression (r = -44, p < .001), and mindfulness was negatively associated with depression (r = -72, p < .001). Mindfulness showed full mediation in the relationship between stressful life events and depression. Conclusion: Stressful life events influenced the nursing students' depression via the full mediating effect of mindfulness. Promoting mindfulness plays an important role in reducing depression in nursing students. Therefore, it is important to develop a mindfulness program or use a mindfulness-based stress reduction program (MBSR) for nursing students.

Discovering Temporal Relation Considering the Weight of Events in Multidimensional Stream Data Environment (다차원 스트림 데이터 환경에서 이벤트 가중치를 고려한 시간 관계 탐사)

  • Kim, Jae-In;Kim, Dae-In;Song, Myung-Jin;Han, Dae-Young;Hwang, Bu-Hyun
    • The Journal of the Korea Contents Association
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    • v.10 no.2
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    • pp.99-110
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    • 2010
  • An event means a flow which has a time attribute such as a symptom of patient. Stream data collected by sensors can be summarized as an interval event which has a time interval between the start-time point and the end-time point in multiple stream data environment. Most of temporal mining techniques have considered only the frequent events. However, these approaches may ignore the infrequent event even if it is important. In this paper, we propose a new temporal data mining that can find association rules for the significant temporal relation based on interval events in multidimensional stream data environment. Our method considers the weight of events and stream data on the sensing time point of abnormal events. And we can discover association rules on the significant temporal relation regardless of the occurrence frequency of events. The experimental analysis has shown that our method provide more useful knowledge than other conventional methods.

An Efficient Multiple Event Detection in Sensor Networks (센서 네트워크에서 효율적인 다중 이벤트 탐지)

  • Yang, Dong-Yun;Chung, Chin-Wan
    • Journal of KIISE:Databases
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    • v.36 no.4
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    • pp.292-305
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    • 2009
  • Wireless sensor networks have a lot of application areas such as industrial process control, machine and resource management, environment and habitat monitoring. One of the main objects of using wireless sensor networks in these areas is the event detection. To detect events at a user's request, we need a join processing between sensor data and the predicates of the events. If there are too many predicates of events compared with a node's capacity, it is impossible to store them in a node and to do an in-network join with the generated sensor data This paper proposes a predicate-merge based in-network join approach to efficiently detect multiple events, considering the limited capacity of a sensor node and many predicates of events. It reduces the number of the original predicates of events by substituting some pairs of original predicates with some merged predicates. We create an estimation model of a message transmission cost and apply it to the selection algorithm of targets for merged predicates. The experiments validate the cost estimation model and show the superior performance of the proposed approach compared with the existing approaches.

A Study on the Constructions of Fire Events Probabilistic Safety Assessment Model for Nuclear Power Plants (원자력발전소의 화재사건 확률론적안전성평가 모델 구축에 관한 연구)

  • Kang, Dae Il;Kim, Kilyoo
    • Journal of the Korean Society of Safety
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    • v.31 no.5
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    • pp.187-194
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    • 2016
  • A single fire event within a fire area can cause multiple initiating events considered in internal events probabilistic safety assessment (PSA). For an example, a fire event in turbine building fire area can cause a loss of the main feed-water and loss of off-site power initiating events. This fire initiating event could result in special plant responses beyond the scope of the internal events PSA model. One approach to address a fire initiating event is to develop a specific fire event tree. However, the development of a specific fire event tree is difficult since the number of fire event trees may be several hundreds or more. Thus, internal fire events PSA model has been generally constructed by modifications of the pre-developed internal events PSA model. New accident sequence logics not covered in the internal events PSA model are separately developed to incorporate them into the fire PSA model. Recently, many fire PSA models have fire induced initiating event fault trees not shown in an internal event PSA model. Up to now, there has been no analytical comparative study on the constructions of fire events PSA model using internal events PSA model with and without fault trees of initiating events. In this study, the changing process of internal events PSA model to fire events PSA model is analytically presented and discussed.

Influencing Factors on Turnover Intention of Nurses in Emergency Department (응급실 간호사의 이직의도 영향요인)

  • Maeng, Su Youn;Sung, Mi Hae
    • Korean Journal of Occupational Health Nursing
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    • v.24 no.2
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    • pp.86-93
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    • 2015
  • Purpose: This study was to identify the factors determining the turnover intention of nurses in Emergency Department (ED). Methods: The subjects were 123 ED nurses working at 10 general hospitals in Busan, Korea. The data were collected from August 15th to September 22nd, 2013. The collected data were analyzed using the SPSS program through descriptive statistics, t-test, ANOVA, Pearson's correlation coefficients and stepwise multiple regression. Results: There were positive correlations between traumatic events experience and post-traumatic stress (r=.416, p<.001), between depression and traumatic events experience (r=.212, p=.001), between traumatic events experience and turnover intention (r=.289, p=.001), between post-traumatic stress and depression (r=.251, p=.005), and also between depression and turnover intention (r=.315, p<.001). Factors influencing turnover intention were depression and traumatic events experience with $R^2$ value 16.7%. Conclusion: Considering these results, it seems that the important factors determining the turnover intention of nurses in ED are depression and traumatic events experienced by nurses. Therefore, an active plan is needed to develop strategies for reducing nurses' depression and traumatic events experienced by nurses.

Optimal supervisory control for multiple-modelled discrete event systems

  • Lee, Moon-Sang;Lim, Jong-Tae
    • 제어로봇시스템학회:학술대회논문집
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    • 2001.10a
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    • pp.73.5-73
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    • 2001
  • In this paper, we present a procedure to design the robust optimal supervisor which has the minimal cost in the sense of average for a given multiple-modelled discrete event system DES. In order to design the robust optimal supervisor, we extend the optimal supervisor design algorithm for a deterministic DES to the case of multiple-modelled DESs. In addition, using the proposed algorithm with modified costs of events and penalities of states, we can show whether a robust supervisor for a given multiple-modelled DES exists and design the minimally restricted robust supervisor.

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Analysis on Hypothetical Multiple Events of mSGTR and SBO at CANDU-6 Plants Using MARS-KS Code (중수로 원전 가상의 mSGTR과 SBO 다중 사건에 대한 MARS-KS 코드 분석)

  • Seon Oh YU;Kyung Won LEE;Kyung Lok BAEK;Manwoong KIM
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.17 no.1
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    • pp.18-27
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    • 2021
  • This study aims to develop an improved evaluation technology for assessing CANDU-6 safety. For this purpose, the multiple steam generator tube rupture (mSGTR) followed by an unmitigated station blackout (SBO) in a CANDU-6 plant was selected as a hypothetical event scenario and the analysis model to evaluate the plant responses was envisioned into the MARS-KS input model. The model includes logic models for controlling the pressure and inventory of the primary heat transport system (PHTS) decreasing due to the u-tubes' rupture, as well as the main features of PHTS with a simplified model for the horizontal fuel channels, the secondary heat transport system including the shell side of steam generators, feedwater and main steam line, and moderator system. A steady state condition was successfully achieved to confirm the stable convergence of the key parameters. Until the turbine trip, the fuel channels were adequately cooled by forced circulation of coolant and supply of main feedwater. However, due to the continuous reduction of PHTS pressure and inventory, the reactor and turbine were shut down and the thermal-hydraulic behaviors between intact and broken loops got asymmetric. Furthermore, as the conditions of low-flow coolant and high void fraction in the broken loop persisted, leading to degradation of decay heat removal, it was evaluated that the peak cladding temperature (PCT) exceeded the limit criteria for ensuring nuclear fuel integrity. This study is expected to provide the technical bases to the accident management strategy for transient conditions with multiple events.

Cooperative Surveillance and Boundary Tracking with Multiple Quadrotor UAVs (복수 쿼드로터 무인기를 이용한 협업 감시 및 경계선 추종)

  • Lee, Hyeon Beom;Moon, Sung Won;Kim, Woo Jin;Kim, Hyoun Jin
    • Journal of Institute of Control, Robotics and Systems
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    • v.19 no.5
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    • pp.423-428
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    • 2013
  • This paper investigates a boundary tracking problem using multiple quadrotor UAVs to detect and track the boundary of physical events. We set the boundary estimation problem as a classification problem of the region in which the physical events occur, and employ SVL (Support Vector Learning). We also demonstrate a velocity vector field which is globally attractive to a desired closed path with circulation at the desired speed and a virtual phase for stabilizing the collective configuration of the multiple quadrotors. Experimental results with multiple quadrotors show that this study provides good performance of the collective boundary tracking.