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Isolation and Characterization of Transcriptional Elements from Corynebacterium glutamicum

  • Park, Soo-Dong;Lee, Sang-Nam;Park, Ik-Hyun;Choi, Jong-Su;Jeong, Wol-Kyu;Kim, Youn-Hee;Lee, Heung-Shick
    • Journal of Microbiology and Biotechnology
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    • v.14 no.4
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    • pp.789-795
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    • 2004
  • A promoter-probe shuttle vector pSK1Cat was constructed for the isolation of transcriptional signal sequences from Corynebacterium glutamicum. Besides conferring resistance to kanamycin in Escherichia coli and C. glutamicum, the vector carried a promoterless cat gene to confer resistance to chloramphenicol upon insertion of the appropriate transcriptional signals in the multiple cloning site. By utilizing the vector, a series of transcriptionally active fragments were isolated from the genome of C. glutamicum. The clones, ranging from 200 bp to 1 kb in size, were grouped into 3 classes of strong, medium, and weak, based on the chloramphenicol acetyltransferase (CAT) activity and sensitivity to the chloramphenicol of the clone-carrying C. glutamicum cells. C. glutamicum cells carrying the $P_{19}$ clone, a representative in the strong class, were able to grow on minimal agar plates containing over $40 mg/mell$ chloramphenicol, and showed CAT activity of 10 m㏖/mgㆍmin, performing slightly better than the cells carrying $P_{tac}$ , a strong E. coli promoter. Subcloning analysis of the $P_{19}$ clone identified a 180 bp intergenic fragment ($P_{180}$), which was located upstream of a gene encoding a hypothetical membrane protein. The expression conferred by $P_{180}$ was not affected by either the kinds of carbon sources or changes in temperature. These properties make the $P_{180}$ clone useful for the deregulated expression of biosynthetic genes in C. glutamicum during amino acid fermentation.

Detection of Wildfire-Damaged Areas Using Kompsat-3 Image: A Case of the 2019 Unbong Mountain Fire in Busan, South Korea

  • Lee, Soo-Jin;Lee, Yang-Won
    • Korean Journal of Remote Sensing
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    • v.36 no.1
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    • pp.29-39
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    • 2020
  • Forest fire is a critical disaster that causes massive destruction of forest ecosystem and economic loss. Hence, accurate estimation of the burned area is important for evaluation of the degree of damage and for preparing baseline data for recovery. Since most of the area size damaged by wildfires in Korea is less than 1 ha, it is necessary to use satellite or drone images with a resolution of less than 10m for detecting the damage area. This paper aims to detect wildfire-damaged area from a Kompsat-3 image using the indices such as NDVI (normalized difference vegetation index) and FBI (fire burn index) and to examine the classification characteristics according to the methods such as Otsu thresholding and ISODATA(iterative self-organizing data analysis technique). To mitigate the salt-and-pepper phenomenon of the pixel-based classification, a gaussian filter was applied to the images of NDVI and FBI. Otsu thresholding and ISODATA could distinguish the burned forest from normal forest appropriately, and the salt-and-pepper phenomenon at the boundaries of burned forest was reduced by the gaussian filter. The result from ISODATA with gaussian filter using NDVI was closest to the official record of damage area (56.9 ha) published by the Korea Forest Service. Unlike Otsu thresholding for binary classification,since the ISODATA categorizes the images into multiple classes such as(1)severely burned area, (2) moderately burned area, (3) mixture of burned and unburned areas, and (4) unburned area, the characteristics of the boundaries consisting of burned and normal forests can be better expressed. It is expected that our approach can be utilized for the high-resolution images obtained from other satellites and drones.

Prevalence and Risk Factors of Myofascial Pain Syndrome on School Boys (청소년들에서 근막동통증후군의 유병률과 위험요인)

  • Lim, Hyun-Sul;Lee, Jong-Min;Kim, Duck-Soo
    • Journal of Preventive Medicine and Public Health
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    • v.33 no.2
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    • pp.184-192
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    • 2000
  • Objectives : To inquire the prevalence and the risk factors for myofascial pain syndrome (MPS) on young boys in order to use these results as the fundamental data for the prevention of their MPS. Methods : For 7 days in May 1999, this research was taken on 489 male students ranging from 6th to 12th grade. We randomly selected a class for every group and from these classes we operated physical examinations, self-reported questionnaires and from a rehabilitation doctor, MPS test was taken. Thoracic kyphosis and lumbar lordosis were also taken by using the inclinometer. We defined MPS as a regional pain complaint, palpable taut band that is painful on compression. Results : The shoulder MPS prevalence of the subjects were 29.7 persons/100 persons. The statistics revealed that as grades went up, the percentage significantly increased in the MPS prevalence. As of case-control study, 145 students who were tested postive in all aspects were placed as cases, and 176 students who were perfectly normal as controls on risk factors. As a result of comparing the student groups who were stisfied with their chairs to the student groups were not satisfied, the taller showed a significantly higher odds ratio (p<0.01). By the multiple logistic regression test, we concluded that the MPS disease was prevailed far more in the students in the higher grades (Odds ratio: 1.16, 95% C.I.: 1.03-1.31), and also those who were dissatisfied with their chairs than in the ones who were satisfied (Odds ratio: 1.92, 95% C.I.: 1.17-3.17). Conclusions : Significant correlations showed between the MPS diagnosed group and the students who are dissatisfied with their chairs. As a result, more research and observation has to be made concerning this disease, and the desks and chairs should be adjusted to suit the student's physical standards.

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A Comparative Analysis on Educational Achievement in Mathematics Classifying by Content Areas of the Primary and Secondary School Students (초.중.고등학생의 수학과 내용 영역별 학업성취도 비교 분석 -2003~2008년 국가수준 학업성취도 평가 결과를 중심으로-)

  • Lee, Bong-Ju
    • Journal for History of Mathematics
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    • v.23 no.4
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    • pp.59-82
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    • 2010
  • The Purpose of this study is to draw a few lessons for the effective teaching and learning throughout a comparative analysis on the results of the educational achievement on Mathematics of the primary and secondary school students. The primary sources for this research are based on the results of the six-times national level tests performed annually by all level students from 2003 to 2008. In order to achieve this goal, I, firstly, extract the lowest content area in terms of the annual average of the right answer ratio after examining the ratio of right answers to each math problem by classifying all multiple-choice questions of the educational achievement tests from 2003 to 2008 into the relevant content areas. Next, the characteristics of the content area which distinguish the lowest right answer ratio are qualitatively analyzed. Lastly, information on the content area which the school students of all classes feel very difficult to solve is provided via reckoning the average right answer ratio per each content area against all math questions at the last six-times of the national level tests.

Comparison of the Health Insurance Systems of South Korea and Peru

  • Kim, Yanghee;Tantalean-Del-Aguila, Martin;Dronina, Yuliya;Nam, Eun Woo
    • Health Policy and Management
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    • v.30 no.2
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    • pp.253-262
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    • 2020
  • Background: The public health care system of a country is shaped and driven by its historical background as well as social, economic, and cultural structures. This study sheds light on the unique features, strengths, and weaknesses of the health insurance systems of South Korea (Korea) and Peru. Methods: The capacity mapping tool was used to explore the Korean and Peruvian population and geographical structures; health insurance laws, regulations, and policies; payment systems; eligibility and contribution collection; and long-term care insurance. Results: The study found that the Korean government took the lead in integrating multiple insurers into a single-payer system in an effort to reinforce and stabilize its health insurance system in 2000. Peru has been developed mixed model such based on taxes and contributions, to address a gap between different social classes. Peruvian government developed a two-axis system, one for low-income earners, financed by taxes, and another financed by contributions paid by workers and government officials in the formal sector. Peru has introduced many variations to its fee payment and insurer systems, target population, and coverage scope, and maintains its health insurance system accordingly to this day. Conclusion: The current study provides observation of the Health Insurance System in two different countries and helps to understand possible ways to improve the health insurance system in both countries. Based on this study, Peru will be able to see how its system differs from Korea's and benefit from the related policy implications.

Correlation between the Inclinations of the Incisors and Lips in Mixed Dentition (혼합치열기 아동의 전치 경사도와 입술 경사도 사이의 상관관계)

  • Oh, Sungdong;Lee, Jewoo;Kim, Jinyoung;Ra, Jiyoung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.45 no.1
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    • pp.21-31
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    • 2018
  • This study aimed to investigate the correlation between the inclinations of the incisors and lips in mixed dentition aged 7 - 11 years. Lateral cephalometric radiographs of 353 children were analyzed to measure the upper incisor inclination, lower incisor inclination, lower-nasolabial angle, and mentolabial angle in relation to the skeletal malocclusion classification. The measurements were then assessed using the Kruskal-Wallis test, Pearson correlation, and multiple regression analysis. There were significant negative correlations between the inclination of the upper incisors and lower-nasolabial angle in all classes of skeletal malocclusion. There was a negative correlation between the inclination of the lower incisors and mentolabial angle; however, class II malocclusion had a significant positive correlation. This study identified the factors that affect lip inclination and verified their associations.

Spectrum Sensing with Diversity Combining Technique in Cognitive Radio (인지 라디오 시스템에서 다이버시티 기법을 사용한 스펙트럼 검출)

  • Lee, So-Young;Kim, Eun-Cheol;Cha, Jae-Sang;Park, Yong-Woon;Hwang, Sung-Ho;Kim, Ki-Hong;Min, Joon-Ki;Kim, Seong-Kweon;Cho, Ju-Pill;Kim, Jin-Young;Kang, Jang-Mook
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.9 no.3
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    • pp.179-185
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    • 2009
  • Cognitive radio (CR), which is proposed as a technology that utilizes the frequency resources effectively, has studied to relive scarcity of the frequency resources. CR provides opportunistically unused frequency to the secondary user when the primary user is not detected. Spectrum sensing is the most important technology to detect primary user. However, in the wireless channels, according to the effect of multipath fading channel, spectrum sensing performance is compromised. Therefore, in this paper, we apply diversity scheme that is a useful technique for combating multiple fading in wireless communications. There are several classes of diversity scheme, which are time diversity, antenna diversity, muitipath diversity, frequency diversity, and so on. In this paper, we adopt antenna diversity that is a kind of space diversity. By using the proposed method, we can overcome fading effect and improve spectrum sensing performance.

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Relationship between the self-efficacy and CAD/CAM practice of dental technology students (치기공과 학생들의 자기효능감과 CAD/CAM 실습과의 관계)

  • Nah, Jung-Sook
    • Journal of Technologic Dentistry
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    • v.42 no.3
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    • pp.281-289
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    • 2020
  • Purpose: This study analyzes the self-efficacy and CAD/CAM practice of dental technology students with the aim of providing data that will be useful for improving CAD/CAM education. Methods: A questionnaire survey on self-efficacy and CAD/CAM practice was given to students who participated in face-to-face classes in the Department of Dental Technology at a college in Daegu and Gyeongsangnam-do. The survey period was two weeks long (June 15, 2020 to July 2, 2020), and 84 students completed the questionnaire. Results: The higher the self-efficacy, the fewer difficulties or mistakes students made in CAD/CAM practice. The most difficult parts of the scanning process were the top and bottom arc operation and the bite match arc insertion. The most difficult part of the design work was the use of (multiple) tools. The difference between the degree of difficulty and the degree of mistakes during the practical course was shown as the margin line setting in the design work. Setting the margin line was not difficult, but the degree of mistakes was nonetheless high. In the CAM process, height control was the most difficult part, but the degree of mistakes did not differ according to students' self-efficacy. Conclusion: Given that higher self-efficacy correlated with fewer difficulties or mistakes in CAD/CAM practice, students should be encouraged to enhance their self-efficacy. This may be done by improving their satisfaction with their major course of study, forming friendly relations with their colleagues, and increasing the ratio of performance rather than observation in CAD/CAM practice.

A Study on Factors Affecting Cultural Competency of General Hospital Nurses (종합병원 간호사의 문화적 역량수준과 영향요인)

  • Chae, Duck-Hee;Park, Yun-Hee;Kang, Kyeong-Hwa;Lee, Tae-Hwa
    • Journal of Korean Academy of Nursing Administration
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    • v.18 no.1
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    • pp.76-86
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    • 2012
  • Purpose: As Korea has been rapidly transformed to be a multicultural society, it becomes essential for nurses to develop cultural competency. The purpose of this descriptive study was to assess the cultural competency level of general hospital nurses and to examine its contributing factors adopting ecological model. Method: A convenience sample of 327 nurses from six general hospitals in Seoul metropolitan area was recruited between November 14 to 28, 2011. Cultural competency was measured using the Korean version of the Caffrey Cultural Competence in Healthcare Scale (CCCHS) and Cultural Competence Assessment(CCA). Hierarchical multiple regression analysis was performed to assess the influence of intrapersonal, cultural-experience, and organizational factors on cultural competency. Results: Mean cultural competency in this study was 2.57(${\pm}0.43$). Cultural competency was associated with job position (p=.044) at the intrapersonal level. At the cultural experience level, foreign language classes experience (p=.005), fluency in a foreign language (p=.000), experience of working with foreign health care professionals (p=.000) were associated cultural competency. At the organizational level, training program (p=.000), job description (p=.002), health education materials (p=.016), insufficient job control (p=.000), and interpersonal conflicts (p=.011) showed significant difference. Foreign language class experience, foreign language fluency, experience of working with foreign health care professionals, and job control were the most consistent predictors of cultural competence and explained 20.4% of the total variance in the proposed ecological model. Conclusions: The findings suggest that to enhance cultural competency in general hospital nurses, cultural experience and organizational factors need to be more fully considered than intrapersonal factors.

A Rule-Based Image Classification Method for Analysis of Urban Development in the Capital Area (수도권 도시개발 분석을 위한 규칙기반 영상분류)

  • Lee, Jin-A;Lee, Sung-Soon
    • Spatial Information Research
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    • v.19 no.6
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    • pp.43-54
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    • 2011
  • This study proposes a rule-based image classification method for the time-series analysis of changes in the land surface of the Seongnam-Yongin area using satellite-image data from 2000 to 2009. In order to identify the change patterns during each period, 11 classes were employed in accordance with statistical/mathematic rules. A generalized algorithm was used so that the rules could be applied to the unsupervised-classification method that does not establish any training sites. The results showed that the urban area of the object increased by 145% due to housing-site development. The image data from 2009 had a classification accuracy of 98%. For method verification, the results were compared to land-cover changes through Post-classification comparison. The maximum utilization of the available data within multiple images and the optimized classification allowed for an improvement in the classification accuracy. The proposed rule-based image-classification method is expected to be widely employed for the time-series analysis of images to produce a thematic map for urban development and to monitor urban development and environmental change.