• 한국멀티미디어학회논문지
• /
• 제17권2호
• /
• pp.143-151
• /
• 2014

악천후로 인하여 화질이 저하된 영상은 사물의 경계에 해당하는 에지 부분이 흐려진다. 본 논문에서는 에지를 최대한으로 선명하게 하여 영상의 시인성을 향상 시키는 화질 개선 방법을 제안한다. 우선 영상의 밝기 필드에서 극점들을 찾아 에지 후보 영역으로 선택하고, 선택된 에지의 측면에 있는 화소들의 밝기로 히스토그램을 형성한다. 형성된 히스토그램의 극소점을 기반으로 히스토그램을 다수의 모드로 분해한다. 모드가 구해지면, 영상 필드에서 에지에 의하여 연결된 모드들을 구하고, 연결된 모드들의 연결 고리를 구한다. 최종적으로 가장 긴 연결 고리를 형성하는 모드간의 간격을 최대한으로 벌린다. 이 때 최소 밝기 모드와 최대 밝기 모드는 화소 밝기 범위 이내에 있어야 한다. 이와 같이 모드의 간격을 벌림으로써 에지를 선명하게 하고 영상의 시인성을 향상한다. 본 논문에서 제안한 방법은 적은 연산량으로 기존의 방법만큼 좋은 성능으로 화질을 개선함을 보여준다.

• 한국미생물·생명공학회지
• /
• 제46권1호
• /
• pp.29-39
• /
• 2018

Vibrio vulnificus needs various responsive mechanisms to survive and transmit successfully in alternative niches of human and marine environments, and to ensure the acquisition of steady energy supply to facilitate such unique life style. The bacterium had genetic constitution very different from that of Escherichia coli regarding metabolism of glycogen, a major energy reserve. V. vulnificus accumulated more glycogen than other bacteria and at various levels according to culture medium and carbon source supplied in excess. Glycogen was accumulated to the highest level in Luria-Bertani (3.08 mg/mg protein) and heart infusion (4.30 mg/mg protein) complex media supplemented with 1% (w/v) maltodextrin at 3 h into the stationary phase. Regarding effect of carbon source, more glycogen was accumulated when maltodextrin (2.34 mg/mg protein) was added than when glucose or maltose (0.78.1-14 mg/mg protein) was added as an excessive carbon source to M9 minimal medium, suggesting that maltodextrin metabolism might affect glycogen metabolism very closely. These results were supported by the analysis using the malP (encoding a maltodextrin phosphorylase) and malQ (encoding a 4-${\alpha}$-glucanotransferase) mutants, which accumulated much less glycogen than wild type when either glucose or maltodextrin was supplied as an excessive carbon source, but at different levels (3.1-80.3% of wild type glycogen). Therefore, multiple pathways for glycogen metabolism were likely to function in V. vulnificus and that responding to maltodextrin might be more efficient in synthesizing glycogen. All of the glycogen samples from 3 V. vulnificus strains under various conditions showed a narrow side chain length distribution with short chains (G4-G6) as major ones. Not only the comparatively large accumulation volume but also the structure of glycogen in V. vulnificus, compared to other bacteria, may explain durability of the bacterium in external environment.

• 기술혁신학회지
• /
• 제8권spc1호
• /
• pp.360-390
• /
• 2005

The paradigm of the corporate innovations has been changed from the intra-company innovations to the inter-company innovations. A prevalent approach to the inter-company innovations is the supply chain management. Three key words of the core concept of supply chain management are the long-term relationship, resource integration, and value creation. Specifically, it means that the supply chain management aims to make value creation through the resource integration for the supply chain entities, based on the long-term relationship between buyers and sellers. To make more effective long-term relationship among the supply chain entities, it is very important for the supply chain entities to analyze followings: i) What variables can influence the long-term relationship, ii) How these variables can influence to the long-term relationship. However, previous researches mostly deals the long-term relationship in the marketing area in fragment, and thus few research efforts have been done for the development of conceptual model using supply chain management theories. In contrast to previous studies, our research tried to develop and examine the integrative research model by introducing both the marketing theories and the supply chain management theories, and thus related hypotheses are derived. A multiple regression analysis was performed to examine the influence of the antecedents of the long-term relationship, for the 87 retailers of grocery supply chains. The empirical results confirm that cultural similarity, reputation, interdependency, and trust positively influence long-term relationship (i.e., partnership orientation and partnership symmetry). And results also confirm that the supply implementation factors such as organization integration, information system integration, and process integration playa moderating role between antecedents and long-term relationship. These findings suggest that companies should perceive the importance of managing the process, organization, information system integration in the long-term relationship implementation process as well as the factors such as cultural similarity, reputation, interdependency, and trust in the long-term relationship establishment process.

• 대한전자공학회논문지SD
• /
• 제44권2호
• /
• pp.127-138
• /
• 2007

본 논문에서는 최대길이 의사무작위 이진 시퀀스(m-시퀀스)의 쉬프트-덧셈 특성에 근거한 위상천이를 이용하여 회로 출력에 나타나는 X-값을 효과적으로 마스크 함으로써 내장된 자체 테스트를 실현할 수 있는 기법을 제안한다. 이 기법은 패턴생성기인 LFSR의 출력을 적절하게 위상천이 하여 마스크 패턴을 생성할 수 있는 위상천이 네트워크를 이용한다. 테스트 절차 동안에 각 스캔 체인에 인가되는 마스크 패턴의 위상 천이 수는 재구성 가능하다. LFSR의 출력을 적절하게 위상 천이하여 모든 스캔 체인 마스크 패턴을 생성할 수 있는 위상천이 네트워크 합성 알고리즘을 제안한다. 본 논문에서 제안하는 X-마스크 회로는 각 스캔 체인 마스크 패턴을 생성할 수 있는 후보 위상천이 수가 많기 때문에 하드웨어 오버헤드를 효과적으로 감축할 수 있다. 실험을 통하여 제안된 위상천이를 이용한 X-마스크 회로는 기존의 연구 결과보다 훨씬 적은 저장공간과 하드웨어 오버헤드를 필요로 함을 증명한다.

• Clinical and Experimental Pediatrics
• /
• 제63권8호
• /
• pp.314-320
• /
• 2020

Background: The accumulation of unpaired α-globin chains in patients with β-thalassemia major may clinically create ineffective erythropoiesis, hemolysis, and chronic anemia. Multiple blood transfusions and iron overload cause cellular oxidative damage. However, α-tocopherol, an antioxidant, is a potent scavenger of lipid radicals in the membranes of red blood cells (RBCs) of patients with β-thalassemia major. Purpose: To evaluate the effects of α-tocopherol on hemolysis and oxidative stress markers on the RBC membranes of patients with β-thalassemia major. Methods: Forty subjects included in this randomized controlled trial were allocated to the placebo and α-tocopherol groups. Doses of α-tocopherol were based on Institute of Medicine recommendations: 4-8 years old, 200 mg/day; 9-13 years old, 400 mg/day; 14-18 years old, 600 mg/day. Hemolysis, oxidative stress, and antioxidant variables were evaluated before and after 4-week α-tocopherol or placebo treatment, performed before blood transfusions. Results: Significant enhancements in plasma haptoglobin were noted in the α-tocopherol group (3.01 mg/dL; range, 0.60-42.42 mg/dL; P=0.021). However, there was no significant intergroup difference in osmotic fragility test results; hemopexin, malondialdehyde, reduced glutathione (GSH), or oxidized glutathione (GSSG) levels; or GSH/GSSG ratio. Conclusion: Use of α-tocopherol could indirectly improve hemolysis and haptoglobin levels. However, it played no significant role in oxidative stress or as an endogen antioxidant marker in β-thalassemia major.

• 한국정보통신학회논문지
• /
• 제18권4호
• /
• pp.992-999
• /
• 2014

단백질이 존재하는 세포내의 다중 위치를 정확하게 예측하기 위하여 다중레이블 학습 방법을 광범위하게 비교한다. 이를 위하여 다중레이블 분류의 접근 방법인 알고리즘 적응, 문제 변환, 메타 학습의 여러 방법을 비교 평가한다. 다양한 관점에서 다중레이블 분류 방법의 특성을 평가하기 위하여 12가지 평가 척도를 사용하였고, 최적의 성능을 보이는 방법을 찾기 위하여 새로운 요약 척도를 사용하였다. 비교 실험 결과, 흔하지 않은 다중레이블 집합을 가지치기 하는 멱집합 방법과, 관련 레이블들을 추가된 특징으로 나타내는 분류기-체인 방법의 성능이 높았다. 또한, 이들 방법들로 구성된 여러 개의 분류기를 조합하면 더욱 성능이 향상되었다. 즉, 세포내 위치간의 연관관계를 사용하는 것이 예측에 효과적인데, 특정 생물학적 기능을 수행하는 단백질의 세포내 위치들의 관계는 독립적이지 않고 서로 관련되어 있기 때문이라 판단된다.

• Journal of Microbiology and Biotechnology
• /
• 제27권11호
• /
• pp.1983-1993
• /
• 2017

Salmonella enterica subsp. enterica serovar Typhimurium, one of the most common foodborne pathogens, is transmitted mainly through contaminated food derived from infected animals. In this study, S. Typhimurium ST1120, an isolate from pig feces in Korea, was subjected to whole-genome analysis to understand its genomic features associated with virulence. The genome of ST1120 was found to have a circular chromosome of 4,855,001 bp (GC content 52.2%) and a plasmid of 6,863 bp (GC content 46.0%). This chromosome was predicted to have 4,558 open reading frames (ORFs), 17 pseudogenes, 22 rRNA genes, and 86 tRNA genes. Its plasmid was predicted to have three ORFs. Comparative genome analysis revealed that ST1120 was phylogenetically close to S. Typhimurium U288, a critical isolate in piggery farms and food chains in Europe. In silico functional analysis predicted that the ST1120 genome harbored multiple genes associated with virulence and stress resistance, including Salmonella pathogenicity islands (SPIs containing SPI-1 to SPI-5, SPI-13, and SPI-14), C63PI locus, ST104 prophage locus, and various antibiotic resistance genes. In accordance with these analysis results, ST1120 showed competence in invasion and survival abilities when it was added to host cells. It also exhibited robust resistance against antibiotics in comparison with other S. Typhimurium strains. This is the first report of the complete genome sequence of S. Typhimurium isolated from swine in Korea. Comparative genome analysis between ST1120 and other Salmonella strains would provide fruitful information toward understanding Salmonella host specificity and developing control measures against S. Typhimurium infection.

• Molecules and Cells
• /
• 제42권12호
• /
• pp.850-857
• /
• 2019

The Gram-negative opportunistic pathogen, Pseudomonas aeruginosa, has multiple multidrug efflux pumps. MexT, a LysR-type transcriptional regulator, functions as a transcriptional activator of the MexEF-OprN efflux system. MexT consists of an N-terminal DNA-binding domain and a C-terminal regulatory domain (RD). Little is known regarding MexT ligands and its mechanism of activation. We elucidated the crystal structure of the MexT RD at 2.0 Å resolution. The structure comprised two protomer chains in a dimeric arrangement. MexT possessed an arginine-rich region and a hydrophobic patch lined by a variable loop, both of which are putative ligand-binding sites. The three-dimensional structure of MexT provided clues to the interacting ligand structure. A DNase I footprinting assay of full-length MexT identified two MexT-binding sequence in the mexEF-oprN promoter. Our findings enhance the understanding of the regulation of MexT-dependent activation of efflux pumps.

• 대한두경부종양학회지
• /
• 제19권2호
• /
• pp.133-136
• /
• 2003

Background and Objectives: Kikuchi's disease or subacute necrotizing lymphadenitis is a cause of persistently enlarged lymph nodes unresponsive to antibiotic therapy. It affects predominantly young women under the age of 30, and it is seen primarily in the Asian population. Although this disease usually follows a benign course, all describing a clinical entity that has been mistaken for malignant lymphoma, lupus, and an assortment of infectious diseases. The purpose of this study is to report clinical characteristics and treatment outcome in order to contribute to the precise diagnosis and treatment. Materials and Methods: We reviewed 27 cases, who were diagnosed as subacute necrotizing lymphadenitis on excisional biopsy during the past 5 years from January 1998 to December 2002. Results: It occurred more often in females (20 cases ; 74%) than males (7cases ; 26%), and it was seen more frequently in the second and third decades (21 cases ; 78%). Cervical lymphadenopathy were usually multiple (24 cases, 89%) and measured less than 2cm (20 cases, 74%). The posterior cervical and deep jugular chains are the most common location(34 cases ; 81%). Leukopenia(18 cases, 67%) and elevated ESR(20 cases, 75%) were commonly noted in laboratory data. Conclusions: It is easy that Kikuchi's disease is mistaken for malignant lymphoma. So we should consider fine needle aspiration or open biosy of lymph node for histologic diagnosis in patients who have localized cervical lymphadenopathy unresponsive to antibiotic therapy.

• Molecules and Cells
• /
• 제22권1호
• /
• pp.97-103
• /
• 2006

Phosphoinositides are critical regulators of ion channel and transporter activity. There are multiple isomers of biologically active phosphoinositides in the plasma membrane and the different lipid species are non-randomly distributed. However, the mechanism by which cells impose selectivity and directionality on lipid movements and so generate a non-random lipid distribution remains unclear. In the present study we investigated which structural elements of phosphoinositides are responsible for their subcellular location and movement. We incubated phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PI(4)P) and phosphatidylinositol 4,5-bisphosphate ($PI(4,5)P_2$) with short or long acyl chains in CHO and HEK cells. We show that phosphate number and acyl chain length determine cellular location and translocation movement. In CHO cells, $PI(4,5)P_2$ with a long acyl chain was released into the cytosol easily because of a low partition coefficient whereas long chain PI was released more slowly because of a high partition coefficient. In HEK cells, the cellular location and translocation movement of PI were similar to those of PI in CHO cells, whereas those of $PI(4,5)P_2$ were different; some mechanism restricted the translocation movement of $PI(4,5)P_2$, and this is in good agreement with the extremely low lateral diffusion of $PI(4,5)P_2$. In contrast to the dependence on the number of phosphates of the phospholipid head group of long acyl chain analogs, short acyl chain phospholipids easily undergo translocation movement regardless of cell type and number of phosphates in the lipid headgroup.