• Korean Journal of Agricultural Science
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• v.26 no.1
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• pp.71-76
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• 1999

The methanolic extracts from the elm (Ulmus davidiana var japonica) root barks which have been known as an oriental medicine Eubackpie for the treatment of edema, ulcer and inflammation were examined for their bacteriocidal effects on mastitis pathogens of milk cows. The growth of the pathogenic bacteria such as Staphylococcus aureus and Streptococcus sp. No. 12 was markedly inhibited by the chloroform fraction among the extracts, and these antibacterial activities were also supported by MIC test. Water-soluble mucilage was obtained from the root barks with a yield of 36.8~40.2% by dry weight basis. This substance appeared to be a kind of polysaccharide with $61.0{\pm}1.0%$ carbohydrate content, and to have hygroscopic and astringent properties due to its high viscosity and water absorption.

• Preventive Nutrition and Food Science
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• v.17 no.1
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• pp.36-45
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• 2012

To produce novel cheese-like fermented soybean, the solid-state fermentation of roasted soybean flour (RSF) was performed using 1.0% inoculum Bacillus subtilis HA and Lactobacillus plantarum, with the initial 60% substrate moisture for 10 hr at $42^{\circ}$, resulting in pH 6.5, 0.82% acidity, 3.5% mucilage, 14.3 unit/g protease activity, 7.6 unit/g fibrinolytic activity, 216 mg% tyrosine content and $1.7{\times}10^{10}$ CFU/g of viable cell counts. After the second lactic acid fermentation with 10~30% skim milk powder, the fermented RSF resulted in an increase in acidity with 1.64~1.99%, tyrosine content with 246~308 mg% and protease activity in the range of 5.2~17.5 unit/g and 0.966 water activity. Viable cell counts as probiotics indicated $1.6{\times}10^8$ CFU/g of B. subtilis and $7.3{\times}10^{10}$ CFU/g of L. plantarum. The firmness of the first fermented RSF with 2,491 $g{\cdot}{\o}mm^{-1}$ greatly decreased to 1,533 $g{\cdot}{\o}mm^{-1}$ in the second fermented RSF, although firmness was slightly increased by adding a higher content of skim milk. The consistency of the second fermented RSF also decreased greatly from 55,640 to 3,264~ 3,998 in the presence of 10~30% skim milk. The effective hydrolysis of soy protein and skim milk protein in the fermented RSF was confirmed. Thus, the second fermented RSF with a sour taste and flavor showed similar textural properties to commercial soft cheese.

• Journal of Korean Society on Water Environment
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• v.34 no.2
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• pp.210-215
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• 2018

It is difficult to count colonial cyanobacteria Microcystis cells since the thickness of colonies is constrained by amorphous mucilage, making it impossible to estimate the number of cells. Disaggregation of Microcystis colonies into single cell is needed to improve the accuracy and precision of cell density estimation of naturally collected samples. Uultrasonic treatment method is commonly used owing to the simplicity and immediacy of the procedure. However, amplitude, frequency, and duration of ultrasonic treatment also cause cell loss during the experiment. Optimal ultrasonic treatment has not been standardized yet. Therefore, the objective of this study was to investigate optimal ultrasonic treatment by analyzing cell density and colony numbers. We collected colonial Microcystis from Changnyeong-Haman weir area in Nakdong River during harmful algal boom period from September to October in 2017. Ultrasonic treatment method was applied to disrupt colonies into single cells to enumerate cell density. Among treatment conditions, results from continuously treated for 100 seconds were found to be the optimum to reduce colonies to a suspension of single cell without cell losses under high and low density of Microcystis cells. Lugol iodine fixed cells followed by sonication showed less negative impact of cell damage within the optimal treatment time (100 seconds). Furthermore, disaggregated cells treated by sonication enables microscopic observation more easily since gas vacuoles were collapsed to facilitate sedimentation of cells under the counting chamber for quantitative enumeration of buoyant Microcystis cells.

• The Plant Pathology Journal
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• v.19 no.2
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• pp.92-96
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• 2003

The study was conducted to determine the cultural conditions and the effect of inert fillers for melanization and sporulation abilities of sodium alginate pellets, and the weeding efficacy of the formula in the field. Melanin production of E. nematosporus was affected by striking frequency. Percentage of melanized beads was increased to 80.6% at higher rpm up to 180. The melanized pellets produced more conidia with abundant mucilage than unmelanized pellets. Shaker culture of Epicoccosorus nematosporus with sodium alginate yielded a total of 55 mg per 100 pellets. Percentage of melanized pellets was highest with 81.0% and 83.3% of melanization, when wheat bran and rice polish were amended and produced the conidia with 65.4 and 68.4 mg per 100 pellets, respectively. When 1 L of conidial suspension of 6.0$\times$$10^5$ conidia per ml was applied on 30-day-old plants in a plot, 74.5% of the plants were killed within 20 days, whereas, its melanized sodium alginate pellets killed 57.8% of the plants in the same period. The number of tuber formation of Eleocharis kuroguwai in the untreated control plots was 128.5 per plot, but those of the plots treated with conidial suspension and melanized pellets were 22.1 and 39.7, respectively, at the end of the season. Results of this study showed that melanization of mycelia-mixed sodium alginate are an important sporulation factor in E. namatosporus as a mycoherbicide.

• The Plant Pathology Journal
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• v.33 no.4
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• pp.410-423
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• 2017

Pathological interrelations of two soil-borne diseases in cucurbits (watermelon, oriental melon, shintosa and cucumber) caused by Fusarium isolates (FI) and the root-knot nematode (RKN), Meloidogyne incognita were characterized by the fusarium disease severity index (DI), RKN gall index (GI) and eggmass index (EI) in inoculation tests using FI and RKN. Virulence of FI as determined by DI at 4 weeks after inoculation was mostly in the higher order of Fusarium proliferatum F6, F5 and Fusarium oxysporum f. sp. melonis or Fusarium oxysporum f. sp. niveum with no significant differential interactions among the cucurbits and RKN co-infection. Significant increases of DI due to RKN coinfection were noticed in watermelon and oriental melon infected with F. proliferatum isolates, suggesting the DI increase due to RKN coinfection may depend upon the virulence of FI relative to aggressiveness of RKN on the cucurbits. For the coinfection of FI and RKN, GI and EI were mostly reduced logarithmically with the increase of DI, largely more in EI than GI, in all cucurbits except for shintosa. Microscopic examination of the root tissues showed histopathological features characteristic to infection types; formation of fungal hyphae and/or spores and plant defense structures (tyloses and mucilage) in variable degrees and formation of giant cells at variable developmental stages and with variable cytoplasmic depletion or degeneration which were visualized in relations with the values of DI, GI and EI. These findings will be helpful to develop control strategies of the soil-borne disease complex based on their pathological characteristics.

• Applied Microscopy
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• v.39 no.1
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• pp.57-64
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• 2009

The trapping leaves of Drosera capture insects by secreting sticky mucilage from numerous glandular trichomes (GTs) that are developed on the leaf epidermis. The present study examines and compares the structural features of those trichomes in Drosera binata and D. pygmy with the use of light and electron microscopy. The study focuses primarily on the development and differentiation pattern of the GTs during growth. Upon examination, the upper and lower epidermis were readily distinguishable by the features of GTs in developing leaves. In particular, the GTs were dense in the upper epidermis and along the leaf margin. In D. binata, the capitate GTs with elongated stalk and sessile peltate GTs were found most commonly, whereas only capitate GTs with varying degrees of the stalk length were observed in D. pygmy. Up to ca. $2.2{\sim}3.4\;mm$ long capitate GTs were seen in the leaf margins of D. binata and ca. $3.7{\sim}4.2\;mm$ long GTs having racket-like head with adaxial hemispheric structures, otherwise known as tentacles, were noted in the leaf margin of D. pygmy. The peltate GTs were found to be distributed in the lower epidermis of D. binata. In both species, head cells were dense with cytoplasm containing high numbers of Golgi bodies, ER, mitochondria and small vesicles. Secretory materials accumulated within numerous small vacuoles, then fused together to form a single large vacuole, which serves as a secretory cavity. Flection movement of the marginal GTs and leaf blade GTs, and increased mucilage secretion from the head cells upon contact with prey during the capturing process are considered to be major factors in their active insectivorous mechanism. The findings of this study will be useful in comparisons to similar findings in other species that form adhesive trapping leaves, such as Drosophyllum or Pinguicula., further contributing a better understanding of the function and structure of the trapping leaves of carnivorous plants.

• Journal of Korean Society of Forest Science
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• v.78 no.1
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• pp.42-54
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• 1989

These studies were carried out to examine the anatomical, karyological characteristics, the variation of isozymes in needle and the frost damage of tissue for the purpose of investigating the relationships among the three natural Torreya nucifera populations of Cheju-do, Namhae, and Mt. Naejang in Korea. The results obtained can be summed up as follows : 1. The numbers of endodermal cells and inner layer cells of mucilage canal of needle conductive tissue were different in each population. The number of those of Cheju-do population was the largest. 2. In somatic chromosome, Namhae population showed one more secondary constriction. The values of $b^{arm}/a^{arm}$ were the same in all the three populations, but Cheju-do population particularly showed a different minimum value. And the karyotype formulas of each population showed difference. 3. During the meiosis, each population showed no significant difference in the irregular phenomena of chromosome. 4. In isoperoxidase and esterase variations of needle, each population showed its particular number and variation of bands. Cheju-do population showed the largest number and greatest variation of bands. 5. Under the same freeging conditions, the frost damage of Mt. Naejang population was comparatively slight, and Cheju-do population suffered from a greater frost damage than the others.

• Korean Journal of Environmental Biology
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• v.31 no.2
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• pp.113-120
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• 2013

This study aimed to investigate the effects of ultrasonic wave on colonial changes of Microcystis sp. causing water-bloom. The effects of frequency change and exposure time of ultrasonic wave on Microcystis sp. were also perceived by measuring the concentration of chlorophyll-a. The concentrations of chlorophyll-a showed a decreasing tendency in all experimental groups exposed to ultrasonic waves, especially the highest decrease at 70 kHz. Before exposed to ultrasonic wave, single cells of Microcystis sp. were observed in a form of colonies which were densely accumulated onto their mucilage layer. However, after one hour exposure to 70 kHz ultrasonic wave, the bonds of colonies were weakened and single cells were separated from the colonies. Moreover, the single cells of the colony were destroyed with the 5 hour exposure time at 70 kHz. As a result, we proposed that the frequency strength and the exposure time of ultrasonic wave might have effects in decrease of biomass of blue-green algae by reducing the concentration of chlorophyll-a, thereby changing and destroying forms of Microcystis sp. colonies.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.1
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• pp.95-102
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• 2012

To examine the new practical utilization of mucilages in Opuntia humifusa, the Korean pear cactus, the polysaccharides were isolated from O. humifusa, and immuno-stimulating activities were assayed. The main polysaccharide, CNC-E, was prepared by a commercial enzyme treatment, water extraction, and ethanol precipitation. The molecular mass of CNC-E was estimated to be about 700 kDa, and it consisted mainly of arabinose, galactose and xylose in addition to two minor sugars such as rhamnose and fucose. On the other hand, CNC-E showed considerably high splenocyte proliferation activity in a dose-dependent manner. Peritoneal macrophages stimulated with CNC-E produced cytokines such as IL-6, IL-12, IL-10, and TNF-${\alpha}$. The intravenous administration of CNC-E significantly augmented the cytotoxicity of natural killer (NK) cells against Yac-1 tumor cells. Especially, NK cells obtained from the mice treated with $100{\mu}g$ of CNC-E showed threefold higher cytolytic activity than those of untreated mice. CNC-E also showed potent anti-complementary activity in a dose-dependent fashion. Identification of C3 activation products by the crossed immunoelectrophoresis using anti-human C3 and the anti-complementary activity of CNC-E in a $Ca^{2+}$-free condition suggested complement activations by CNC-E that occur via both alternative and classical pathways. These results indicate that Korean pear cactus contains selected polysaccharides that provide immuno-stimulating activities beneficial to human health.

• Applied Microscopy
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• v.40 no.4
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• pp.237-244
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• 2010

Utricularia forms small, but complex carnivorous trap along the stem either in water or in soil depending upon species. The shapes and sizes of the traps, appendages, and trichomes are known to differ among aquatic, terrestrial and epiphytic species. In the present study, the morphology and microstructure of the trap in aquatic Utricularia japonica and terrestrial U. livida were examined using light and electron microscopy. The aim of this study was to compare the characteristics of trap features between the aquatic and terrestrial species. The trap was found to be comprised of a thin walled bladder with numerous capitate trichomes, two-armed bifid and four-armed quadrifid glands in both species; however, the traps of the two species were different in size, and number and morphology of the trichomes and glands. Aquatic Utricularia was chlorenchymatous with chloroplasts distributed throughout the body, whereas the terrestrial species was translucent without plastids due to an adaptation to underground habitats. Furthermore, the former differed considerably in that the traps developed antenna and appendages around the entrance area. A peculiar trap entrance was also noted in U. livida, which exhibited radiating rows of various trichomes within funnel-shaped tissue. A large number of glandular trichomes covered the entrance area and door surface with four trigger hairs each in the aquatic form but only two in the terrestrial form. The glandular trichomes near the door secreted a large amount of mucilage that temporarily composed the velum in the U. japonica, however, it was not observed in the terrestrial species. All of the aforementioned features were highly related in their structure and function during carnivorous mechanism in Utricularia. The current findings provide important data for further comparison of the different life forms within Utricularia.