The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Weeding Efficacy of Melanized Formula with Epicoccosorus nematosporus on Eleocharis kuroguwai in the Field

  • Hong, Yeon-Kyu (National Yeongnam Agricultural Experiment Station（NYAES）, Rural Development Adminstration（RDA)) ;
  • Cho, Jae-Min (National Yeongnam Agricultural Experiment Station（NYAES）, Rural Development Adminstration（RDA) ;
  • Uhm, Jae-Youl (Agricultural Biology Division, Kyungpook National University) ;
  • Hyun, Jong-Nae (National Yeongnam Agricultural Experiment Station（NYAES）, Rural Development Adminstration（RDA) ;
  • Lee, Bong-Choon (National Yeongnam Agricultural Experiment Station（NYAES）, Rural Development Adminstration（RDA) ;
  • Song, Seok-Bo (National Yeongnam Agricultural Experiment Station（NYAES）, Rural Development Adminstration（RDA) ;
  • Lee, Dong-Chang (National Yeongnam Agricultural Experiment Station（NYAES）, Rural Development Adminstration（RDA))
  • Published : 2003.04.01
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Abstract

The study was conducted to determine the cultural conditions and the effect of inert fillers for melanization and sporulation abilities of sodium alginate pellets, and the weeding efficacy of the formula in the field. Melanin production of E. nematosporus was affected by striking frequency. Percentage of melanized beads was increased to 80.6% at higher rpm up to 180. The melanized pellets produced more conidia with abundant mucilage than unmelanized pellets. Shaker culture of Epicoccosorus nematosporus with sodium alginate yielded a total of 55 mg per 100 pellets. Percentage of melanized pellets was highest with 81.0% and 83.3% of melanization, when wheat bran and rice polish were amended and produced the conidia with 65.4 and 68.4 mg per 100 pellets, respectively. When 1 L of conidial suspension of 6.0$\times$$10^5$ conidia per ml was applied on 30-day-old plants in a plot, 74.5% of the plants were killed within 20 days, whereas, its melanized sodium alginate pellets killed 57.8% of the plants in the same period. The number of tuber formation of Eleocharis kuroguwai in the untreated control plots was 128.5 per plot, but those of the plots treated with conidial suspension and melanized pellets were 22.1 and 39.7, respectively, at the end of the season. Results of this study showed that melanization of mycelia-mixed sodium alginate are an important sporulation factor in E. namatosporus as a mycoherbicide.

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References

  1. Agrios, G. N. 1978. Plant Pathology, 2nd. ed. Academic Press, New York, 703 p
  2. Allen, E. A., Hoch, H. C., Steadman, J. R. and Stavely, R. J. 1991. Influence of leaf surface features on spore deposition and the epiphytic growth of phytopathogenic fungi. pp. 87-110, In: Microbial Ecology of Leaves. J. H. Andrews and S. S. Hirano, eds. Springer-Verlag, New York
  3. Boyette, C. D. and Walker, H. L. 1985. Production and storage of inoculum of Cercospora kikuchii for field studies. Phytopathology 75:183-185 https://doi.org/10.1094/Phyto-75-183
  4. Boyette, C. D. and Walker, H. L. 1986. Evaluation of Fusarium lateritium as a mycoherbicide for controlling velvetleaf (Abutilon theophrasti) and prickly sida (Sida spinosa). Weed Sci. 34:106-109
  5. Chida, T. and Sisler, H. D. 1987a. Restoration of appressorial penetration ability by melanin precursors in Pyricularia oryzae treated with antipenetrants and in melanin deficient mutants. J. Pestic. Sci. 12:49-55 https://doi.org/10.1584/jpestics.12.49
  6. Chida, T. and Sisler, H. D. 1987b. Effects of inhibitors of melanin biosynthesis on appressorial penetration and reductive reactions in Pyricularia oryzae. Pestic. Biochem. Physiol. 29:244-251 https://doi.org/10.1016/0048-3575(87)90154-4
  7. Fravel, D. R., Marois, J. J., Lumsden, R. D. and Connick, W. J., Jr. 1985. Encapsulation of potential biocontrol agents in an alginate-clay matrix. Phytopathology 75:774-777 https://doi.org/10.1094/Phyto-75-774
  8. Hong, Y. K., Kim, J. C., Ryu, K. R. and Kim, S. C. 1991. Pathogenicity and some related characteristics of the fingerprint blight pathogen (Epicoccosorus nematosporus) attacking water chestnut (Eleocharis kuroguwai Ohwi). Korean Plant Pathology Newsletter 2:47 (abstract)
  9. Hong, Y. K., Kim, J. C. and Lee, S. K. 1992. Biological control of rice weed, water chestnut (Eleocharis kuroguwai), using the fingerprint stem blight pathogen (Epicoccosorus nematosporus). Korean Plant Pathology Newsletter 3:75 (abstract)
  10. Hong, Y.K., Cho, J. M., Kim, J. C. and Uhm, J. Y. 1995. Identification, pathogenicity and host range of a potential bioherbicide, Epicoccosorus nematosporus, causing fingerprint stem blight on water chestnut, Eleocharis kuroguwai. Korean J. Plant Pathol. 12:58-65
  11. Hong, Y. K., Cho, J. M., Kim, J. C. and Uhm, J. Y. 1996. Identification, pathogenicity and host range of a potential Epicoccosorus nematosporus, causing fingerprint stem blight on water chestnut, Eleocharis kuroguwai. Korean J. Plant Pathol. 12:58-65
  12. Hong, Y. K., Cho, J. M., Uhm, J. Y. and Ryu, K. R. 1997. Potential application of Epicoccosorus nematosporus for the control of water chestnut. Korean J. Plant Pathol. 13:167-171
  13. Hong, Y. K., Shin, D. B., Song, S. B., Lee, B. C. and Lee, D. C. 2001. Effect of some pesticides on the fungus Epicoccosorus nematosporus and synergistic effect in combination with herbicides on Eleocharis kuroguwai control in rice paddy field. Kor. J. Weed Sci. 21:365-372
  14. Hong, Y. K., Cho, J. M., Lee, B. C., Uhm, J. Y. and Kim, S. C. 2002a. Factors affecting sporulation, germination and appressoria formation of Epicoccosorus nematosporus as a mycoherbicide under controlled environment. Plant Pathol. J. 18:50-53 https://doi.org/10.5423/PPJ.2002.18.1.050
  15. Hong, Y. K., Cho, J. M., Ryu, K. L., Shin, D. B. and Kim, S. C. 2002b. The suitable cultural conditions for inoculum production of Epicoccosorus nematosporus as a mycoherbicide agent. Kor. J. Weed Sci. 22:61-66
  16. Hong, Y. K., Hyun, J. N., Cho, J. M., Uhm, J. Y. and Kim, S. C. 2002c. Factors affecting sporulation of a mycoherbicide, Epicoccosorus nematosporus, on the lesion of Eleocharis kuroguwai. Plant Pathol. J. 18:81-84 https://doi.org/10.5423/PPJ.2002.18.2.081
  17. Hyakumachi, M., Yokoyama, K. and Ui, T. 1987. Role of melanin in susceptibility and resistance of Rhizoctonia solani to microbial lysis. Trans. Br. Mycol. Soc. 89:155-159 https://doi.org/10.1016/S0007-1536(87)80147-X
  18. Kim, K. U. and Kwon, S. T. 1985. Bud sprouting and tuberization of Eleocharis kuroguwai Ohwi. Kor. Weed Sci. 5:43-49
  19. Kubo, Y., Furusawa, I. and Yamamoto, M. 1984. Regulation of melanin biosynthesis during appressorium formulation in Colletotrichum lagenarium Exp. Mycol. 8:364-369 https://doi.org/10.1016/0147-5975(84)90060-4
  20. Lewis, J. A. and Papavizas, G. C. 1985. Characteristics of alginate pellets formulated with Tricoderma and Gliocladium and their effect on the proliferation of the fungi in soil. Plant Pathol. 34: 571-577 https://doi.org/10.1111/j.1365-3059.1985.tb01409.x
  21. Ormeno-Nunez, J., Reeleder, F. D. and Watson, A. K. 1988. A foliar disease of field bindweed (Convolvulus arvensis) caused by Phomopsis convolvulus. Plant Dis. 72:338-342 https://doi.org/10.1094/PD-72-0338
  22. Papavizas, G. C. and Lewis, J. A. 1979. Side effect of pesticides on soil-born plant pathogens. pp. 451-534 In: Soilborn Plant Pathogens, B. Schippers and W. Gams., eds. Academic Press, New York
  23. Swan, D. G. 1983. Regeneration of field bindweed (Convolvulus arvensis) seedlings. Weeds Today 14:3-41
  24. Suzuki, K., Kubo, Y., Furusawa, I., Ishida, N. and Yamamoto, M. 1982. Behavior of colorless appressoria in an albino mutant of Colletotrichum lagenarium. Can. J. Microbiol. 28:1210-1213 https://doi.org/10.1139/m82-180
  25. Walker, H. L. 1981. Granular formulation of Alternaria macrospora for control of spurred anoda (Anoda cristata) Weed Sci. 29:342-345
  26. Walker, H. L. and Connick, W. J. Jr. 1983. Sodium alginate for production and formulation of mycoherbicides. Weed Sci. 31: 333-338
  27. Weidemann, G. J. 1988. Effect of nutritional amendments on conidial production of Fusarium solani f. sp. cucurbitae on sodium alginate granules and control of Texas gourd. Plant Dis. 72:757-759 https://doi.org/10.1094/PD-72-0757
  28. Weidemann, G. J. and Templeton, G. E. 1988. Efficacy and soil persistence of Fusarium solani f. sp. cucurbitae for control of Texas gourd (Cucurbita texana). Plant Dis. 72:36-38 https://doi.org/10.1094/PD-72-0036