• 제목/요약/키워드: mu acupuncture treatment

검색결과 150건 처리시간 0.026초

대식세포에서 산화질소 생성에 대한 당귀(當歸) 에탄올 추출물의 억제효과 (Inhibitory Effect of Angelica gigas Nakai Extract on Nitric Oxide Production in RAW 264.7 Cells)

  • 정미영;박히준;정지행;김진용;강전모;이나경;임사비나
    • 대한한의학회지
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    • 제28권2호통권70호
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    • pp.155-165
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    • 2007
  • Objective : The Angelica gigas Nakai ethanol extract (AGE) was investigated to compare nitric oxide (NO) production and $NF-{\kappa}B$ activity from RAW 264.7 cells, since NO and nuclear $factor-{\kappa}B$ $(NF-{\kappa}B)$ have been shown to be factors implicated in inflammatory disease. Method : AGE was prepared by extracting medicinal herb with 70% (v/v) ethanol solution. We investigated production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) gene expression by ARE in LPS-stimulated RAW 264.7 macrophage cells. We also investigated inhibition of LPS-induced activation of $NF-{\kappa}B$ on western blot. Result : LPS-induced RAW 264.7 cells increased NO production and iNOS expression. Upon treatment with AGE, nitrite production was significantly inhibited in a concentration-dependent manner compared to the untreated control. AGE inhibited this LPS-induced iNOS mRNA and protein in a dose-dependent manner. AGE markedly inhibited the expression of iNOS mRNA and protein at a concentration of 100 ${\mu}g/ml$. LPS-induced RAW 264.7 cells with AGE blocked inhibitory $factor-{\kappa}B{\alpha}$ degradation. Conclusion :This study shows that AGE seems to attenuate inflammation through inhibition of NO production and iNOS expression by blockade of $NF-{\kappa}B$ activation in LPS-stimulated RAW 264.7 cells.

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추위에 노출된 슬관절염 모델에서 내슬안, 외슬안 직접구가 통증행동과 TRPM8 발현에 미치는 영향 (Effects of Direct Moxibustion Applied to EX-LE4 and EX-LE5 on the Pain Behavior and Expression of TRPM8 in the Rat Model of Ambient Cold Exposed Osteoarthritis)

  • 지병욱;김이꽃;이지은;구성태
    • Korean Journal of Acupuncture
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    • 제33권4호
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    • pp.204-212
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    • 2016
  • Objectives : The aim of the study is to investigate the effects of moxibustion on the pain behavior and expression of TRPM8 in the dorsal root ganglion(DRG) in the rat model of ambient cold(AC) exposed osteoarthritis(OA). Methods : OA was induced by the injection of $50{\mu}l$ of 2% monosodium iodoacetate(MIA) into the knee joint cavity. To examine the level of pain, weight bearing forces(WBFs) of affected limb was measured. For the AC exposure, the animals were housed in 6 h/day at $4^{\circ}C$ for 14 days after MIA injection. Moxibustion treatment was performed at EX-LE4 and EX-LE5 with 5 cons(1, 7 or 10 mg) per day for 13 days from 5 days after MIA injection. The expressions of TRPM8 in DRG were measured by western blotting analysis. Results : The WBFs of MIA-AC group were decreased significantly compared to MIA group at 2, 3, 6, 7, 8 and 9 days after arthritis induction. After the first 6 h-AC exposure, expressions of TRPM8 in MIA-AC group were increased significantly compared to those of naive group. After moxibustion treatment, only the WBFs of 7 mg treated group were restored significantly. Moreover, the over-expressions of TRPM8 were attenuated by the moxibustion treatment in AC exposed rats. Conclusions : The data suggest that AC can increase arthritic knee pain via up-regulated TRPM8 and moxibustion treatment improve the arthritic pain via modulation of TRPM8 expression in DRG in the rat model of AC exposed MIA induced arthritis.

급성 췌장염모델에서 전침의 치료효과 (Therapeutic Effects of Electroacupuncture on Cholecystokinin-octapeptide-induced Acute Pancreatitis Models)

  • 정상수;윤지원;정경아;이종덕;배선준;조장희;성강경
    • Journal of Acupuncture Research
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    • 제22권5호
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    • pp.57-66
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    • 2005
  • Objectives : We examined the effects of electroacupuncture on the cholecystokinin-octapeptide-induced acute pancreatitis in rats. Methods : Rats were administered with $75{\mu}g/kg$ cholecystokinin-octapeptide subcutaneously three times (1, 3 and 5h after shaving) for 5days. Three days after finishing cholecystokinin-octapeptide administration, high frequency electroacupuncture (100Hz) and low frequency electroacupuncture (2Hz) were applied to acupoint equivalent to ST36 (Zusanli) for 7 days. The author determined the pancreatic weight/body weight ratio, the levels of pancreatic heat shock protein HSP60 and HSP72. The author also assay the secretion of ${\beta}-amylase$, lipase and pro-inflammatory cytokines in serum. Repeated cholecysokinin-octapeptide treatment resulted in the typical laboratory and morphological changes of experimentally induced pancreatitis. Results : Eelectroacupuncture significantly decreased the pancreatic weight/body weight ratio in cholecystokinin-octapeptide-induced acute pancreatitis, increased the pancreatic levels of HSP60 and HSP72, and decreased ${\beta}-amylase$ and lipase levels in cholecystokinin-octapeptide-induced acute pancreatitis. Additionally, the secretion of $Interleukin-1{\beta}$ and tumor necrosis $factor-{\alpha}$ was decreased in the animals treated with electroacupuncture. Conclusion : These results suggest that electroacupuncture may have protective effects against cholecystokinin-octapeptide-induced acute pancreatitis.

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Inhibition of COX-2 Activity and Proinflammatory Cytokines($TNF-{\alpha}{\;}and{\;}IL-1{\beta}$) Production by Water-Soluble Sub-Fractionated Parts from Bee (Apis mellifera) Venom

  • Nam, Kung-Woo;Je, Kang-Hoon;Lee, Jang-Hurn;Han, Ho-Je;Lee, Hye-Jung;Kang, Sung-Kil;Mar, Woongchon
    • Archives of Pharmacal Research
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    • 제26권5호
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    • pp.383-388
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    • 2003
  • Bee venom is used as a traditional medicine for treatment of arthritis. The anti-inflammatory activity of the n-hexane, ethyl acetate, and aqueous partitions from bee venom (Apis mellifera) was studied using cyclooxygenase (COX) activity and pro-inflammatory cytokines (TNF-$\alpha and IL-1\beta$) production, in vitro. COX-2 is involved in the production of prostaglandins that mediate pain and support the inflammatory process. The aqueous partition of bee venom showed strong dose-dependent inhibitory effects on COX-2 activity ($IC_{50} = 13.1 \mu$ g/mL), but did not inhibit COX-1 activity. The aqueous partition was subfractionated into three parts by molecular weight differences, namely, B-F1 (above 20 KDa), B-F2 (between 10 KDa and 20 KDa) and BF-3 (below 10 KDa). B-F2 and B-F3 strongly inhibited COX-2 activity and COX-2 mRNA expression in a dose-dependent manner, without revealing cytotoxic effects. TNF-$\alpha and IL-1\beta$ are potent pro-inflammatory cytokines and are early indicators of the inflammatory process. We also investigated the effects of three subfractions on TNF-$\alpha and IL-1\beta$ production using ELISA method. All three subfractions, B-F1, B-F2 and B-F3, inhibited TNF-$\alpha and IL-1\beta$production. These results suggest the pharmacological activities of bee venom on anti-inflammatory process include the inhibition of COX-2 expression and the blocking of pro-inflammatory cytokines (TNF-$\alpha and IL-1\beta$) production.

경락경혈 안진법을 적용한 절경(切經) 방법론에 대한 고찰 (Study on the method of Jeolgyeong using palpation of meridian and acupoint)

  • 유준상
    • 대한한의진단학회지
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    • 제25권1호
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    • pp.72-87
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    • 2021
  • Objectives This is the review on the Jeolgyeong which means diagnosis method of meridian and acupoints. Detecting method of meridian and acupoints under the disease condition using fingertips were more developed in Japan than in China and Korea. This study is the introduction to the Japanese style palpation method on meridian and acupoints. Methods Firstly the textbook 'Acupuncture medicine' published by the committee of Korean professors' association of acupuncture and moxibustion was analyzed. Especially the contents of the palpation method on meridian and acupoints were focused and summarized and secondly the book describing the palpation method on meridian and acupoints was selected, translated and summarized. Results Until now the knowledge on the acupoints reaction depending on the patient's status, the deficiency pattern and the excessive pattern was rarely described in China and Korea. On the contrary, 4 kinds of reactions in the deficiency pattern and 4 kinds of reactions in the excessive pattern were well described in Japanese literatures. Mild touching, mild pinching, rubbing, mild pressing and severe pressing were also described along the meridians. Except the bladder meridian Back Shu points, Yuan points, Luo points, Xi(cleft) points, Front Mu points were depicted as the same as those in China and Korea. For the bladder meridian lines, 4 lines were organized including 2 lines of Hua-Tuo-Jia-Ji-Xue and the middle line between 1st and 2nd bladder meridian line as well as 1st bladder meridian line and 2nd bladder meridian line. Conclusions Detecting meridians or acupoints and using them for diagnosis and treatment is a method used in China, Korea, and Japan. However, detailed descriptions of the method for detecting meridians through palpation are revealed in Japanese books. Palpation methods for diagnosing meridians using touching, pinching, rubbing, and pressing methods are expected to be standardized and systematically learned in Korea.

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Airway Smooth Muscle에 미치는 자울의 효과 (Effect ot Radix Asteris on Airway Smooth Muscle)

  • 나경상;권의광;소응향;서은미;한종현
    • Korean Journal of Acupuncture
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    • 제18권1호
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    • pp.157-164
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    • 2001
  • Radix Asteris has been used in Korea for many centuries as a treatment for respiratory disease. The effect of Radix Asteris on tracheal smooth muscle is not known. The purpose of the present study is to determine the effect of Radix Asteris on histamine induced tracheal smooth muscle contraction in rats and guinea pigs. Guinea pig(500g, male) and Sprague Dawley rats (250g, male) were killed by $CO_2$ exposure and a segment (8-10mm) of the thoracic trachea from each rat and guinea pig was cut into equal segments and mounted 'in pairs' in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of histamine (His) which evoked 50% of maximal response ($ED_{50}$) was obtained from cumulative dose response curves for histamine $(10^{-7}{\sim}10^{-4}M)$. Contractions evoked by His ($ED_{50}$) were inhibited significantly by Radix Asteris. In guinea pig tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 120.5% (p<0.01) after $100{\mu}l/ml$ Radix Asteris. In rat tracheal smooth muscle, the mean percent inhibition of histamine induced contraction was 135.4% (p<0.01) after $100{\mu}l/ml$ Radix Asteris. Propranolol $(10^{-7}M)$ slightly but significantly attenuated the inhibitory effects of Radix Asteris. Following treatment with propranolol, the mean percent inhibition caused by $100{\mu}l/ml$ Radix Asteris fell to 44.6% in guinea pig induced by histamine contraction and by $100{\mu}l/ml$ Radix Asteris fell to 18.7% (p<0.05) in rat induced by histamine contraction. Indomethacin and methylene blue$(10^{-7}M)$ did not significantly alter the inhibitory effect of Radix Asteris. These results indicate that Radix Asteris can relax histamine induced contraction of guinea pig and rat tracheal smooth muscle, and that this inhibition involves sympathetic effects.

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급성기 교통사고 상해 환자의 한약 복용에 관한 인식 조사 (A Survey on Acute Traffic Injury Patient's Perception on Korean Herbal Medicine)

  • 김수연;오승주;황규현;진은석;염선규;김동우;김석우;진소리;박무진;김은송;이현석
    • 한방재활의학과학회지
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    • 제30권4호
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    • pp.165-178
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    • 2020
  • Objectives This survey study is designed to check the awareness and satisfaction of Korean herbal medicine in patients suffering from acute traffic accidents. Methods From May 1 to 20, 2020, patients who visited the hospital within 7 days after the accident and received prescription of Korean herbal medicine for the first-time symptoms were selected. Survey was conducted 10 days after the first visit to confirm the perception of Korean herbal medicine and expected to be used as a prior study to improve the quality of Korean medicine led by Korean herbal medicine. Results Most patients answered that they were aware of the prescribed Korean herbal medicine's dosage and treatment effects. 65% of the patients said they would like to receive other forms of prescription if possible. Most patients answered that portability is convenient and tastes better than normal. 48% of the respondents said that it was the first to time to take Korean herbal medicine, and 68% said that they were willing to take the Korean herbal medicine later. At the time of the first visit, the health condition assessed using EuroQol visual analogue scale (EQ-VAS) was 46.84, but 10 days after the treatment began, it increased significantly to 61.93. Conclusions The improvement of symptoms and high satisfaction with Korean medicine medical services contributed to the patient's willingness to visit the Korean medicine hospital again and to respond that they were willing to take further medication. EQ-VAS elevation suggests that overall patients' subjective health conditions have improved since treatment.

흰쥐 관절염 모델에서 용천 저출력 레이저 자극이 보행행동에 미치는 영향 (Effects of Low Level Laser Treatment Applied to KI 1 on the Gait Behavior in the Rat Model of CFA-Induced Arthritis)

  • 지병욱;이성금;이지은;구성태
    • Korean Journal of Acupuncture
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    • 제32권1호
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    • pp.30-38
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    • 2015
  • Objectives : The aim of the study is to investigate the effects of low level laser treatment (LLLT) on the gait behavior in the rat model of arthritis. Methods : Knee arthritis was induced by the injection of $125{\mu}l$ of Complete Freund's Adjuvant(CFA) into the right hind knee joint cavity. Arthritic rats were divided 3 groups; arthritis group was used for control(CON), 10 min of laser treated group(LSR10), and 30 min of laser treated group(LSR30). LLLT was applied to KI 1 for 11 times under gaseous anesthesia. We performed several analyses under catwalk test including stand and swing time, duty cycle of paw steps, intensity and print area of steps, and stride length. Results : Stand and duty cycle of paw steps were increased significantly at 12 days after arthritis induction in LSR30 group. Swing time was decreased significantly at 12 days after arthritis induction in LSR10 group. In the analysis of intensity, print area and stride length, however, results did not show statistical significance during the time point of experiments. Conclusions : The data suggest that LLLT on the rat model of CFA induced arthritis showed beneficial effects by increase of stand time and duty cycle of paw steps and decrease of swing time. Therefore, LLLT could be useful option to improve gait discomfort in arthritis patients.

프락셔널 레이저 치료와 한의치료를 병행한 욕창치료 1례 (A Case Report on Pressure Ulcer Patient Treated with Fractional Laser Therapy and Korean Medicine)

  • 강자연;이기향;장인수;김홍준;정민정;서형식
    • 한방안이비인후피부과학회지
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    • 제31권4호
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    • pp.126-135
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    • 2018
  • Objectives : The purpose of this report to represent the efficacy of pressure ulcer treatment with fractional laser therapy and Korean medicine. Methods : We treated Parkinsonism patients with pressure ulcer using herbal medicine, fractional mode laser therapy, acupuncture and simple dressing for 3 weeks. Laser was set on fractional mode (on time $100{\mu}s$, dot pitch 0.6 mm, shape RECT) and irradiated around pressure ulcer site and center of pressure ulcer site once a day. The treatment effect was evaluated by The National Pressure Ulcer Advisory Panel(NPUAP) stage and the The Pressure Ulcer Scale for Healing(PUSH) tool(3.0). Results & Conclusions : Pressure ulcer site have improved markedly. NPUAP stage was improved from 2 to 1 and total score of the PUSH tool decreased from 12 to 8. Even though the wound was not cured completely, this shows that fractional laser therapy and Korean medicine could be effective treatment for improving pressure ulcers.

다낭신의 중의치료에 대한 임상연구 동향 - CNKI검색을 중심으로 (A Review of Clinical Studies of Chinese Medicine Treatment of Polycystic Kidney Disease Using the CNKI Database)

  • 주아라;박미소;최요섭;추원정;백혜경;박무진
    • 대한한방내과학회지
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    • 제42권3호
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    • pp.225-238
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    • 2021
  • Objective: This research reviews and investigates the trends in recent clinical studies of polycystic kidney disease (PKD) in China. Method: We searched for clinical studies discussing Oriental medicine-based treatments for PKD in the China National Knowledge Infrastructure (CNKI) database. Thirteen clinical articles published from 2001 to 2019 were analyzed. The search focused on the authors, publication year, type of study, purposes of study, method and duration of treatment, evaluation criteria, and results of the selected articles. Results: Of the articles from the database, 9 case series and 4 randomized controlled trials (RCTs) were analyzed. Ten articles used herbal medicine; 4 used herbal medicine for external use. Gamigyejibokryeong-hwan was the most common herbal prescription. The most frequently used herb was Polia Sclerotium (茯苓), and Cnidii Rhizoma (川芎) was employed in all the external uses. All 13 studies confirmed the efficacy of Oriental medicine treatments. Conclusion: 1. Scientifically designed and more varied clinical studies are required to develop treatments for PKD. 2. The current study could be used as basic data in future clinical studies on treatment and further studies of PKD.