• Korean Journal of Environment and Ecology
• /
• v.28 no.5
• /
• pp.523-530
• /
• 2014

One juvenile raptor which was not able to be identified due to its head damage was discovered on a roadside in Janggok-ri, Jori-eup, Paju on 28th June, 2011. The species was identified by DNA barcoding. After polymerase chain reaction (PCR) of the mitochondrial cytochrome c oxidase subunit I gene (COI), we obtained 695 bp sequences. We analyzed the obtained COI sequence with similar sequences from the BOLD systems and BLAST of the NCBI Genbank, and discovered that its sequence showed 100 % similarity values with the one of the five gray-faced buzzards which were previously researched. In addition, it was confirmed to be a female through sex determination using DNA. Such results are important information as it confirms the breeding of the gray-faced buzzards for the first time in 43 years since its breeding was last recorded in 1968, in Paju. Wildlife rescue center needs to work with adjacent consigned registration and preservation institutions when carcass of wild animals is collected or DNA samples are obtained for more accurate both species and sex identification through a systematic management system in the future. Furthermore, the obtained DNA sample of the gray-faced buzzard and COI gene, DNA barcode, could be used as reference standards for similar researches in the future.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.18 no.3
• /
• pp.131-141
• /
• 2013

Stock identification of Todarodes pacificus collected in the East Sea, Yellow Sea and East China Sea during the period from September to December in 2011 was analyzed by morphometric characters and mitochondrial DNA (mtDNA) cytochrome oxidase subunit I (COI) gene nucleotide variations. Frequency distributions of mantle length was analyzed by morphological method with measuring size of T. pacificus. Then each stock was estimated to confirm their maturation for mean mantle length comparing with mean mature mantle length 20-22 cm. According to morphologic stock identification, it is estimated that the northern part of East Sea is categorized as summer stock and the rest parts, including mid /southern part of the East Sea, northern part of the East China Sea and northern part of the West Sea were autumn stock. For genetic analysis, a total 49 haplotypes were defined by 33 variable nucleotide sites. From the extensive haplotype diversity, limited nucleotide diversity and star-like shape of haplotype network, T. pacificus appears to have undergone rapid population expansion from an ancestral population with a small effective population size. Although pair-wise Fst estimates which represent genetic difference among groups were low, there are relatively remarkable difference of Fst between middle and southern part of the East Sea. Although middle part of the East Sea and southern part of the East Sea were situated at the East Sea, genetically separated groups were appeared.

• Fisheries and Aquatic Sciences
• /
• v.27 no.3
• /
• pp.145-158
• /
• 2024

Life history characteristics, habitat landscape, and historical events are believed to have shaped the patterns of genetic variation in many taxa. The bony lip barb, Osteohilus vittatus, represent a potamodromous fish that complete all life cycle in freshwater and is widely distributed in Southeast Asia. It usually lives in small rivers and other freshwater habitats, and movement between habitats for either food or reproduction has been typical. These life history characteristics may promote gene flow, leading to less structured populations. However, many freshwater habitats are fragmented, which restricts gene flow. We investigate how this interplay has shaped patterns of genetic variation and phylogeographic structure within this species in the Sundaland, a biodiversity hotspot with a complex geological history, using mitochondrial cytochrome oxidase I (mtCOI) as a genetic marker. Forty-six mtCOI sequences of 506 bp long were collected from ten localities, eight geographically isolated and two connected. The sequences were used for population genetic and phylogeographic analyses. Our results showed a low genetic diversity within populations but high between populations. There was a deep phylogeographic structure among geographically isolated populations but a lack of such structure in the connected habitats. Among geographically isolated populations, sequence divergence was revealed, ranging from 1.8% between Java and Sumatra populations to 12.2% between Malaysia and Vietnam. An indication of structuring was also observed among localities that are geographically closer but without connectivity. We conclude that despite high dispersal capacity, the joint effects of historical events, long-term geographic isolation associated with sea level oscillation during the Pleistocene, and restricted gene flow related to lack of habitat connectivity have shaped the phylogeographic structure within the O. vittatus over the Sundaland.

• Korean journal of applied entomology
• /
• v.39 no.1
• /
• pp.43-52
• /
• 2000

A portion of mitochondria1 COI gene (438 bp) was sequenced from the sampls of Plutella xylostella from four localities in Korea to investigate the population genetic structure and characteristics by measuring the magnitude of genetic diversity and the degree of gene flow among populations. Thirteen haplotypes ranging in nucleotide divergence 0.3% to 1.4%, were obtained from 21 individuals. The nucleotide divergence was similar to the other related studies, but haplotype diversity was substantially higher (mean h = 0.81). The genetic distance among geographically remote Cheju Island population and the two Kimhae populations, distant 1 lkm to each other, was not statistically significant (p<0.05). Instead, a substantial or high female gene flow was detected (Nm = 2-30). One Hawaiian haplotype of the diamondback moth obtained through GenBank search also was genetically similar to the ones obtained from this study. Collectively, the genetic population structure of the diamondback moth in Korea can be characterized into two aspects. First, the diamondback moths in Korea possesses overall moderate genetic divergence based on a high number of haplotypes. Second, a high haplotype diversity within each population due to the long distance dispersal with a substantial dispersal power and the resultant genetic similarity among geographic populations is characteristic.

• Proceedings of the Zoological Society Korea Conference
• /
• 2000.10a
• /
• pp.277.1-277
• /
• 2000

No Abstract, See Full Text

• Journal of Species Research
• /
• v.1 no.2
• /
• pp.133-150
• /
• 2012

Amynthas tralfamadore sp. nov. is described from the Mt Halla, Jeju Island biosphere exhibition housed at NIBR Incheon. This species' taxonomy is problematical since it is similar to Amynthas masatakae (Beddard, 1892) from Japan, itself previously reported from Korea and at one time associated with Amynthas campestris (Goto & Hatai, 1898) and A. parvicystis (Goto & Hatai, 1899), all three variously implicated in prior A. robustus (Perrier, 1872) along with Amynthas aspergillum (Perrier, 1872). Based on reinspection of the London lectotype of A. masatakae-here designated and sketched for the first time-the current solution is for maintenance of all five taxa separately. A closely-related Japanese species-Metaphire ryunome sp. nov.-is comparable to Korean Metaphire reisuiensis (Kobayashi, 1938) comb. nov. Another specimen was identified as Amynthas micronarius (Goto & Hatai, 1898), a new record for Korea. It matches the newly-designated neotype (Tokyo NMST An446) and an annotated synonymy is provided; however, erstwhile synonyms, Amynthas shimaensis (Goto & Hatai, 1899) and A. yamizoyamensis (Ohfuchi, 1935) combs. novae, are briefly redescribed and provisionally restored to the Japanese list. mtDNA COI-5P barcode analyses support species identifications.

• Korean journal of applied entomology
• /
• v.48 no.4
• /
• pp.547-551
• /
• 2009

Two closely related species, the soybean podworm, Matsumuraeses phaseoli, and the podborer, M. falcana, gives differential economic damages on crops. It is difficult to discriminate these potential sympatric species by morphological characters. The goal of this study was to develop a discriminating molecular marker based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A partial genomic fragment (500 bp) of mitochondrial cytochrome oxidase I (mtCOI) was sequenced in both species, in which restriction site by Rsa I was selected as a dichotomous marker. PCR-RFLP in the mtCOI region clearly discriminated both species.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.54 no.2
• /
• pp.188-193
• /
• 2021

On March 13, 2018 two postflexion larval specimens (18.28 mm and 16.80 mm in standard length) belonging to the family Cryptacanthodidae, suborder Zoarcoidei were collected from Sokcho and Gangneung in Gangwon Province. The family Cryptacanthodidae comprises 4 species worldwide: 3 in the North Pacific Ocean and 1 in the western North Atlantic Ocean. As a result of analyzing 620 bp of the mtDNA COI region, the two postflexion larvae collected in this study were identified as Cryptacanthodes bergi by 99.5% agreement with C. bergi adult registered in NCBI. Postflexion larvae of C. bergi are compressed with large eyes and radial pectoral fins and the anus located in front of the center of the body. Melanophores were intensively distributed along the dorsal midline, except for caudal peduncle, and sporadically distributed on the back of the anus. In addition, there were limited star-like melanophores on the back of the gut. This species showed 0.058 genetic distance when compared mtDNA COI region of C. aleutensis, and it was well distinguished in the distribution pattern of black vesicles of the head, count and measurement traits. Considering the morphological and ecological characteristics of this species, we suggest a new Korean name, " Gwisin-jang-gaeng-i ".

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.55 no.6
• /
• pp.967-972
• /
• 2022

We investigated the molecular and morphological traits of 338 individual macrourids collected from the Southern Ocean (FAO area number, 88.1 and 88.3) between 2021 and 2022 by Korean bottom trawls. We first identified them as Macrourus caml and Macrourus whitsoni based on morphological traits, such as the number of pelvic fin rays (PF) and the rows of lower jaw teeth (LJT). However four individuals showed uncategorizable morphological characteristics such as PF and LJT numbers that overlapped between the two species. Subsequently, we obtained and analyzed 509 bp of the mtDNA COI sequences of 49 individuals, including the four unidentified individuals, and found only one single nucleotide polymorphism (SNP) that distinguished the two species. Finally, using our molecular identification key, we confirmed that each two individuals were misidentified as M. whitsoni and M. caml reversely. Our results suggest that the number of PF and LJT should be investigated together to accurately identify the two species.

• Journal of Ecology and Environment
• /
• v.44 no.3
• /
• pp.143-154
• /
• 2020

Background: Korean wild boars (Sus scrofa coreanus Heude), because of their adaptability, are a widespread large mammal; however, they sometimes cause problems by invading farms and eating the crops, creating insufficiencies of some foods in South Korea. To understand the diet composition of Korean wild boars according to sex and body size, we collected their feces from Mt. Jeombongsan, Seoraksan National Park, South Korea. The sizes of fecal samples were measured, and genomic DNA was extracted from the samples. We amplified specific loci targeting plants (rbcL and trnL) and animals (COI) to detect the food sources of this omnivore and amplified the ZF and SRY regions to determine the sex. Results: In the wild boar feces, Rosaceae and Bryophyte were the most frequently detected plant food sources at the family level and Diptera and Haplotaxida were the most frequently detected animal food sources at the order level. As a result of sex determination, the sex ratio of wild boars collected in the Mt. Jeombongsan area was approximately 1:1. Our result suggested that there is no significant difference between the diet composition of male and female boars. Based on the average cross-sectional area of the feces, the top 25% were classified into the large body size group and the bottom 25% were classified into the small body size group. The large body size group mainly preferred Actinidiaceae, and the small body size group most frequently consumed Fagaceae. The diet of the large body size group was more diverse than the small body size group. Conclusions: Our results showed that the wild boars preferred Rosaceae, especially Sanguisorba and Filipendula, as plant food sources, and Diptera and Coleoptera of Insecta as animal food sources. Based on the results, the dietary preferences of wild boar appear to be distinguished by not their sex but their body size. Our study could help to elucidate the feeding ecology and population structure of wild boar, as well as address conservation and management issues.