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http://dx.doi.org/10.5656/KSAE.2009.48.4.547

A Molecular Marker Discriminating the Soybean Podworm, Matsumuraeses phaseoli and the Podborer, M. falcana (Lepidoptera: Tortricidae)  

Heo, Hye-Jung (Plant Medicine, School of Bioresource Sciences, Andong National University)
Son, Ye-Rim (Plant Medicine, School of Bioresource Sciences, Andong National University)
Seo, Bo-Yoon (National Institute of Crop Science)
Jung, Jin-Kyo (National Institute of Crop Science)
Kim, Yong-Gyun (Plant Medicine, School of Bioresource Sciences, Andong National University)
Publication Information
Korean journal of applied entomology / v.48, no.4, 2009 , pp. 547-551 More about this Journal
Abstract
Two closely related species, the soybean podworm, Matsumuraeses phaseoli, and the podborer, M. falcana, gives differential economic damages on crops. It is difficult to discriminate these potential sympatric species by morphological characters. The goal of this study was to develop a discriminating molecular marker based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A partial genomic fragment (500 bp) of mitochondrial cytochrome oxidase I (mtCOI) was sequenced in both species, in which restriction site by Rsa I was selected as a dichotomous marker. PCR-RFLP in the mtCOI region clearly discriminated both species.
Keywords
Matsumuraeses phaseoli; Matsumuraeses falcana; PCR; RFLP; Molecular marker;
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Times Cited By KSCI : 4  (Citation Analysis)
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