Browse > Article
http://dx.doi.org/10.13047/KJEE.2014.28.5.523

DNA barcoding of Raptor carcass collected in the Paju city, Korea  

Jin, Seon-Deok (National Science Museum)
Paik, In-Hwan (National Science Museum)
Lee, Soo-Young (National Science Museum)
Han, Gap-Soo (The Korean Association for Bird Protection)
Yu, Jae-Pyoung (National Science Museum)
Paek, Woon-Kee (National Science Museum)
Publication Information
Korean Journal of Environment and Ecology / v.28, no.5, 2014 , pp. 523-530 More about this Journal
Abstract
One juvenile raptor which was not able to be identified due to its head damage was discovered on a roadside in Janggok-ri, Jori-eup, Paju on 28th June, 2011. The species was identified by DNA barcoding. After polymerase chain reaction (PCR) of the mitochondrial cytochrome c oxidase subunit I gene (COI), we obtained 695 bp sequences. We analyzed the obtained COI sequence with similar sequences from the BOLD systems and BLAST of the NCBI Genbank, and discovered that its sequence showed 100 % similarity values with the one of the five gray-faced buzzards which were previously researched. In addition, it was confirmed to be a female through sex determination using DNA. Such results are important information as it confirms the breeding of the gray-faced buzzards for the first time in 43 years since its breeding was last recorded in 1968, in Paju. Wildlife rescue center needs to work with adjacent consigned registration and preservation institutions when carcass of wild animals is collected or DNA samples are obtained for more accurate both species and sex identification through a systematic management system in the future. Furthermore, the obtained DNA sample of the gray-faced buzzard and COI gene, DNA barcode, could be used as reference standards for similar researches in the future.
Keywords
COI GENE; SPECIES IDENTIFICATION; BREEDING RECORD; SEX DETERMINATION; Butastur indicus;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Burger, J. (1993) Metals in avian feathers: bioindicators of environmetnal pollution. Rev. Environ. Toxicol. 5: 203-311.
2 David, M.B., K.L. Bildstein, D.R. Barber and A. Zimmerman (2007). Raptor research and management techniques. Hancock House, 463pp.
3 Frank, B.G. (2006) Ornithology Third Edition. W.H. Freeman and Company, 720pp.
4 Heather, R.L.L (2007) Molecular Phylogenetics of Diurnal Birds of Prey in the Avian Accipitridae Family. Ph.D. thesis, Univ. of Michigan, U.S.A., 125pp.
5 Hebert, P.D.N., A. Cywinska, S.L. Ball and J.R. DeWaard (2003) Biological identifications through DNA barcodes. Proc. R. Soc. Lond. B Biol. Sci. 270: 313-321.   DOI   ScienceOn
6 Ito, H., A. Sudo-Yamaji, M. Abe, T. Murase and T. Tsubota (2003) Sex identification by alternative polymerase chain reaction methods in falconiformes. Zool. Sci. 20:339-344.   DOI   ScienceOn
7 Jin, S.D. (2012) Phylogenetic analysis of birds in Korea using mtDNA variations. Ph.D. thesis, Nat. Univ of Chungnam, Korea., 179pp.
8 Jin, S.D., M.R. Hoque, D.W. Seo, I.K. Kim, C. Jo, W.K. Paek and J.H. Lee (2012) Phylogenetic relationships among Dabbling Duck species in Korea using COI gene variations in mtDNA. J. Poult. Sci. 49:163-170.   DOI
9 Judit, K.S., S.H.M. Butchart, H.P. Possingham and S.T. Garnett (2012) Adapting global biodiversity indicators to the national scale: A red list index for Australian birds. Biol. Conserv. 148: 61-68.   DOI   ScienceOn
10 Kang, S.G., W.H. Hur, Y.S. Choi, J.S. Son, S.H. Han, S.H. Kim and J.H. Yi (2012) Report on the breeding record of Grey-faced Buzzard Butastur indicus. Kor. J. Orni. 19(4) : 255-259. (in Korean with English abstract)
11 Kim, S.H. (2010) Distribution and migration ecology of endangered Accipitridae species in Korea. Ph.D. thesis, Univ. of Chosun, Korea, 177pp. (in Korean)
12 Koike, S. and H. Higuchi (2002) Long-tern trends in the egg-laying date and clutch size of Red-cheeked Starlings. Ibis 144: 15-152.
13 Lee, D.P. (2004) Heavy metal accumulation in some Korean rare bird species. Korean J. Ecol. 27(3): 141-146.(in Korean with English abstract)   DOI   ScienceOn
14 Lee, M.Y., Y.J. Hong, S.K. Park, Y,J. Kim, T.Y. Choi, H.Lee and M.S. Min (2008) Application of two complementary molecular sexing methods for East Asian bird species. Genes Genom. 30(4): 365-372.
15 BirdLife International (2013) Birds are very useful indicators for other kinds of biodiversity. Presented as part of the BirdLife State of the world's birds website. Available from: http://www.birdlife.org/datazone/sowb/casestudy/79.
16 National Institute of Biological Resource (NIBRb) (2011) The study on the restoration and reproduction of endangered raptors(I). Ministry of Environment, Korea,150pp. (in Korean)
17 Milos, V., M. Stevanov-Pavlovic, J. Stevanovic, J. Bosnjak, B. Gajic, N. Aleksic and Z. Stanimirovic (2013) Sex determination in 58 bird species and evaluation of CHD gene as a universal molecular marker in bird sexing. Zoo Biol. 32:269-276.   DOI   ScienceOn
18 Movalli, P.A. (2000) Heavy metal and other residues in feathers of laggar falcon Falco biarmicus jugger form six districts of Pakistan. Environ. Pollut. 109: 267-275.   DOI   ScienceOn
19 National Institute of Biological Resource (NIBRa) (2011) The Workshop on formation of consultative group and establishment of general plan for bird conservation and management in private-public partnership. Ministry of Environment, Korea, pp 67-111.(in Korean)
20 Park, J.G. and J.H. Seo (2008) A Photographic Guide to the Bids of Korea. -Passerine birds-. Shingu press, 514pp. (in Korean)
21 Sakai, S., N. Yamaguchi, H. Momose and H. Higuchi (2011) Seasonal shifts in foraging site and prey of Grey-faced Buzzards (Butastur indicus), breeding in Satoyama habitat of central Japan. Orni. Sci. 10: 51-60.   DOI
22 Satoshi, Y. M., Hiroyuki and H. Hiroyoshi (2004) Dictionary of Ornithology. Showado, 950pp. (in Japanese)
23 Sergio, F. (2003) Relationship between laying dates of black kites Milvus migrans and spring temperatures in Italy. Rapid response to climate change. J. Avian Biol. 34: 144-149.   DOI   ScienceOn
24 Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol. 30: 2725-2729.   DOI   ScienceOn
25 Ueta, M., R. Kurosawa and H. Matsuno (2006) Habitat loss and the decline of Grey-faced Buzzards (Butastur indicus) in Tokyo, Japan. J. Raptor Res. 40:52-56.   DOI   ScienceOn
26 Azuma, A. (2007) Grey-faced Buzzard. Bird Research News. 4(5): 4-5.
27 Hebert, P.D.N., M.Y. Stoeckle, T.S. Zemlak and C.M. Francis (2004) Identification of birds through DNA barcodes. PLoS Biol. 2: e312.   DOI   ScienceOn
28 Both, C., S. Bouwhuis, C.M. Lessells, and M.E. Visser (2006) Climate change and population declines in a long-distance migratory bird. Nature 441: 81-83.   DOI   ScienceOn