• Title/Summary/Keyword: mouse sarcoma 180

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Inhibition of Sarcoma-180 Cell-induced Mouse Ascites Cancer by Astaxanthin-containing Egg Yolks (Sarcoma-180 Cell로 유발한 Mouse 복수암에 대한 Astaxanthin 함유 난황의 효과)

  • 하영래;이상호;박철우;박경아;이영춘;최의성
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.1
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    • pp.163-167
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    • 1998
  • Anticarcinogenic activity of astaxanthin-contatining egg yolk(designate AEY) was investigated for mouse ascites carcinogenesis induced by mouse Sarcoma-180(S-180) cells. Female ICR mice(8mice/treatment, 7∼8weeks of age, 25±1g) were injected, i.p. with S-180 cells(1×107cell/ml PBS). Two days later, each mouse was given 0.1ml PBS containing AEY(10, 25 or 50ug/g body weight) or control egg yolk (CEY; 50ug/g body weight) every other day for 7 times. Control mice were only given 0.1ml S-180 cells and 0.1ml PBS. Mice treated with 25ug/g body weight of AEY showed 24.8 days of life, which was equivalent to 138% of control mice's life(180.0 dyas). Based on dose-dependant experiment of AFY, mice treated with 10ug/g body weight showed slightly longer life(19.4 days) relative to mice treated with control mice, and mice treated with 50ug/g body weight exhibited 21.9 days of life. Mice treated with any dose of AEY exhibited longer life than mice with CEY 50ug/g body weight. Body weight of mice treated with AEY was reduced relative to that of control mice CEY-treated mice. These results suggest than AEY inhibits the carcinogenesis of mouse ascites induced by S-180 cells.

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On the Change of Blood and Serological Pictures of the Sarcoma-180 Mouse and that Mouse treated with Korean Ginseng Extracts (肉腫癌쥐와 高麗人蔘을 處理한 肉腫癌쥐의 血液學的 變化에 관하여)

  • 이유윤
    • The Korean Journal of Zoology
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    • v.23 no.4
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    • pp.229-238
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    • 1980
  • The present experiment was carried out to observe the effect of Korean ginseng extract on the blood picture of mice that have been infected with Sarcoma-180. The observation of standard group was made for 2 days, 7 days, 12 days, 15 days after the injection of Sarcoma-180 and the observation of ginseng extract group was made at 15 days after the injection of Sarcoma-180 and simultaneously with oral adminstration of ginseng extract every day for 15 days. 1. For 15 days after the injection of Sarcoma-180 in the standard group, the numbers of R.B.C. and platlet were not changed significantly, but the number of W.B.C. increased at the first stage, and then decreased suddenly after the middle stage. However, the number of W.B.C. was not severely decreased in the ginseng extract group. This phenomenon is estimated that ginseng extract assisted for W.B.C. to protect living body. 2. Serum protein picture of standard group was appeared as a nonselective protein losing pattern, and ginseng extract group was likely that of standard group, but it was not severe than that of standard group. This phenomenon is believed that ginseng extract gave some delay or immunization for Sarcoma-180 mouse against the development of Sarcoma-180. 3. Serum cholesterol quantities of standard group increased gradually until middle stage, but decreased in the last stage. But serum cholesterol quantity of ginseng extract group showed a slight increase. What the decrease of cholesterol quantity in the last stage of standard group is caused especially by the decreased intaking of feed among many causes, it may be the cause of intaking not decrease of feed in ginseng extract group, as the ginseng extract gave the Sarcoma-180 mouse some resistance against the development of Sarcoma-180. 4. Along with developing of Asrcoma-180 in the standard group, serum total lipid quantities increased, and $\\alpha-, \\beta$-lipoprotein quantities showed decrease phenomenon, but pre $\\beta$-lipoprotein quantities increased rapidly. The value of ginseng extract group was appeared the value in the first stage of standard group. This phenomenon was also estimated that ginseng extract protected the living body according to above theories, and that ginseng extract gave the Sarcoma-180 mouse some resistance against the development of Sarcoma-180.

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Effects of Poria cocos and Panax ginseng Extracts on Hemogram of Sarcoma-180 Mouse (복령 및 인삼추출물이 Sarcoma -180 Mouse의 혈액상(血液象)에 미치는 영향(影響))

  • Lee, Bok-Im;Hong, In-Pyo;Kim, Dong-Won;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.18 no.4
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    • pp.218-224
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    • 1990
  • This study was made to observe changes in hemograms of the Sarcoma-180 mouse treated with extracts of Poria cocos and Panax ginseng. The life span of the Sarcoma-180 mouse administered with the mixture of the extract of Panax ginseng with the extract of P. cocos and the extract of P. cocos was extended more than the non-treated group. In the body weight of the Sarcoma-180 mouse, the non-treated group was increased more than the normal group and the groups administered with the mixture of ginseng extract with hoelen extract and hoelen extract. In the numbers of white blood cell especially lymphocyte, the groups administered with the mixture of ginseng extract with hoelen extract and hoelen extract were similar to the normal group, but the non-treated group was decreased. The Hematocrit, mean corpuscular volume and mean corpuscular hemoglobin in the mice infected with Sarcoma-180 were not significantly different between the group treated with extracts of them and the non-treated group. In the distributions and sizes of white blood cell and platelet in the mice, the group administered with the Hoelen extract was similar to the normal group comparing to the non-treated group.

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Antitumor Effect of Persimmon Leaves in vivo using Sarcoma-180 Cells (Sarcoma-180 세포를 이용한 in vivo에서 감잎의 항암효과)

  • 문숙희;김광혁;박건영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.5
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    • pp.865-870
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    • 1996
  • Antitumor effects of hexane, chloroform fractions and tannins from persimmon leaves were studied by using sarcoma-180 tumor cells. The solid tumor growth was inhibited most effectively when 0.5mg/kg of hexane fraction was administerated to the Balb/c mouse. The life prolongation effects were 35.6%, 11.5% and 29.9%, respectively when hexane fraction, chloroform fraction and tannins from the persimmon leaves were administerated to the mouse. Spleen index, a marker for immunological activity, was increased in mice administrated hexane fraction, chloroform fraction and tannins of persimmon loaves, compared with the control group.

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Antitumor and Immuno-potentiating Activity against Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Tricholoma matsutake (송이(Tricholoma matsutake)의 자실체에서 추출한 조다당류가 생쥐의 Sarcoma 180에 미치는 항암 및 면역증강 작용)

  • Hur, Hyun;Choi, Yon-Il;Lee, Tae-Soo
    • Journal of Life Science
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    • v.18 no.9
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    • pp.1290-1298
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    • 2008
  • Tricholoma matsutake, one of edible and medicinal mushroom belonging to Tricholomaceae of Agaricales, has been known to contain some curing effect on gastric cancer and ulcer, and inhibitory effect on sarcoma 180 and Ehrlich sarcoma. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2, HT29 and NIH3T3 at the concentration of 2.0 mg/ml. Intraperitoneal injection with crude polysaccharides showed life prolongation effect of 23.4$\sim$37.2% in mice previously inoculated with Sarcoma 180. Fr. MeOH and Fr. HW exhibited the immuno-potentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by 2.2$\sim$11.9 folds compared with control at the concentration of 0.2$\sim$0.5 mg/ml. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 6.0 and 1.5 folds at the concentration of 50 mg/kg, respectively. Therefore, it is concluded that crude polysaccharides extracted from fruiting body of Tricholoma matsutake showed antitumor and immuno-potentiating activity against Sarcoma 180 of mouse.

Antitumor Activity of Lactobacillus casei against Sarcoma 180 and Lewis Lung Carcinoma in Mice (생쥐에서 Sarcoma 180 및 Lewis Lung Carcinoma에 대한 Lactobacillus casei의 항암 효과)

  • 배형석;백영진;윤영호
    • Microbiology and Biotechnology Letters
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    • v.21 no.3
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    • pp.247-255
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    • 1993
  • Antitumor activity of Lactobacillus casei YIP 9018(LC9018) was studied in mice by using sarcoma 180(S-180) and Lewis lung carcinoma (3LL). Following the eatablishment of in vivo tumor models such as ascites form S-180, solid form S-180 and 3LL for estimating antitumor activity of Lactobacilli, optimal dose and injection route of heat-killed LC9018 for supperssion of local tumor were examined. Administration of 100ng/mouse of LC9018 significantly inhibited the growth of ascites form S-180, solid form S-180 and 3LL.

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Studies on the Anticancer Effect of Broussonetia kazinoki Extracts (닥나무(Broussonetia kazinoki) 추출물의 항암효과에 관한 연구)

  • 민경진;정승희;구성자
    • Korean journal of food and cookery science
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    • v.15 no.3
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    • pp.231-237
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    • 1999
  • The anticancer effect of the bark of Broussonetia kazinoki root extracts (hexane. chloroform, ethylacetate, butanol, aqueous) were studied. The cytotoxicity by MTT assay and inhibitory effect on the growth of sarcoma 180 cells were tested in vitro. The reduction rate of the tumor formation and spleen/body weight rate on BALB/c mouse were tested in vivo. From the tests, each fraction showed the cytotoxic effect against the sarcoma 180 cells. In addition, as the concentration of the fractions increased, cytotoxic effect tendency increased as well. The cytotoxic rate of the hexane, chloroform, ethylacetate, butanol and aqueous fractions showed by 58.7%, 40.1%, 75.7%, 52.6% and 62.7% respectively after testing by MTT assay system. And sarcoma 180 cells were incubated for 6 days at 37$^{\circ}C$ with various concentrations of each fraction. As the incubation days go on, the number of cells increased, while the inhibition rate on the growth of sarcoma 180 cells were decreased. Especially the ethylacetate fraction at the concentration of 1.0 mg/ml strongly inhibited the growth of sarcoma 180 cells by 74% compared with the control for a day 37$^{\circ}C$ The hexane, chloroform, ethylacetate, butanol and aqueous fractions inhibited on the growth of sarcoma 180 cells by 31%, 19%, 60%, 30% and 42% respectively, when sarcoma 180 cells has been incubated for 6 days at 37$^{\circ}C$. The each fraction exhibited the antitumor effect in vivo. The ethylacetate fraction reduced the tumor formation by 41% compared with the control, when sarcoma 180 cells were injected subcutaneously into the left groin of BALB/c mice. Also spleen/body weight rate of ethylacetate fraction was increased by 2.10% compared with the control (1.08%). And it is considered that there would be no toxic effect caused by each fraction of body weight and organ as there was on more changes in mouse' weight compared with the control.

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Comparison of Lectin from Pseudixus japonicus and Concanavalin a on Lymphocytes Proliferation and Cytotoxicity

  • Chung, Yong-Za;Jung, Hyun-Ok;Hong, Tae-Hong;Suh, Sok-Soo
    • Archives of Pharmacal Research
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    • v.14 no.3
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    • pp.207-216
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    • 1991
  • Pseudixus japonicus agglutinin (PJA) was isolated. And its characteristics were compared with those of concanavalin A (Con A). PJA is a glycopritein composed of 49.3% carbohydrate and 50.7% protein which had relatively high percentages of glutamic acid, aspartic acid and phenylalanine residues. The hemagglutinating activity of PJA was approximately one-eighth of that of Con A when tested with mouse crythrocytes. PJA failed to simulate the proliferation or transformation of human and mouse lymphocytes in contratst to Con A. PJA and Con A showed cytotoxicities against SNU-1 (human stomach cancer cells), SNU-CI (human colon cancer cells) and mouse Sarcoma 180 cells when tested by 3-(4, 5-dimethyl thiazol-2-yl)2. 5-diphenyl tetrazolium bromide (MIT) colorimetric assay. The antitumor activity of the lectin in vivo was also tested in Sarcoma 180 bearing mice. There was no significant difference in prologation of lifc span of the mice after the treatment with PJA and Con A for 10 consecutive days.

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Immuno-stimulating and Antitumor Effects on Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Hericium erinaceus (노루궁뎅이(Hericium erinaceus) 자실체 추출 조다당류의 생쥐 Sarcoma 180에 대한 면역증강 및 항암 효과)

  • Choi, Yon-Il;Lee, Jae-Seong;Lee, U-Youn;Lee, Tae-Soo
    • Journal of Life Science
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    • v.20 no.4
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    • pp.623-631
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    • 2010
  • Hericium erinaceus, an edible and medicinal mushroom belonging to the Basidiomycota family, has been used for curing gastric ulcers and stomach cancers in human beings and is also known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma in mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to as Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from the fruiting body of the mushroom. In in vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cell lines such as Sarcoma 180, HepG2, HT-29 and NIH3T3 at concentrations of $10{\sim}2,000\;{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited a life prolongation effect of 29.1~54.1% in mice previously inoculated with Sarcoma 180. Fr. Na increased the numbers of spleen cells by 2.9 fold at a concentration of $50\;{\mu}g/ml$ compared with the control. Fr. Na improved the immuno-potentiating activity of B lymphocytes by increasing alkaline phosphatase activity by 5.5 fold compared with the control at a concentration of $200\;{\mu}g/ml$. Fr. NaCl increased the numbers of peritoneal exudate cells and circulating leukocytes by 4 and 2.3 folds at a concentration of 50 mg/kg, respectively. Therefore, the crude polysaccharides extracted from the fruiting body of H. erinaceus could improve antitumor activities in mice.

Experimental Study of Naesosan(內消散) on the Effects of Anti-Cancer (內消散의 抗癌效果에 관한 實驗的 硏究)

  • Park, Su-Yeon;Choe, Jeong-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.14 no.1
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    • pp.154-166
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    • 2001
  • Naesosan(NSS) has been used in Oriental Medicine as a drug that treated carbuncle and cellulitis. So, the purpose of this Study was to investigate effects of NSS on the cytotoxicity of cancer cell lines and lymphocytes in vitro, proliferation of Ll210 cells and lymphocytes in L1210 cells transplanted mice, improvement of blood count in Ll210 cells transplanted mice, tumor weight and body weight in sarcoma-180 cells transplanted mice, survival prolongation in sarcoma-180 cells transplanted mice. We used NSS extract with freeze-dried, 8wks-old male mice(balb/c and ICR mouse $18{\pm}2g$). Ll210 cell lines, and sarcoma-180 cell lines for this Study, The proliferation of cells was tested using a colorimetric tetrazoliun assay(MTT assay). The results of this Study were obtained as follows ; 1. NSS showed significantly cytotoxicitic effects of cancer cell lines, did not show cytotoxicitic effects of lymphocytes. 2, Proliferation of lymphocytes in L1210 cells transplanted mice did not effects by NSS. 3. NSS inhibited significantly the proliferation of L1210 cells in L1210 cells transplanted mice. 4. NSS improved significantly the blood count in Ll210 cells transplanted mice. 5. NSS increased significantly th body weight in sarcoma-180 cells transplanted mice. 6. NSS dereased significantly the tumor weight in sarcoma-180 cells transplanted mice. 7. NSS prolonged significantly the survival time in sarcoma-180 cells transplanted mice.

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