Browse > Article
http://dx.doi.org/10.5352/JLS.2010.20.4.623

Immuno-stimulating and Antitumor Effects on Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Hericium erinaceus  

Choi, Yon-Il (Department of Biology, University of Incheon)
Lee, Jae-Seong (Department of Biology, University of Incheon)
Lee, U-Youn (Department of Biology, University of Incheon)
Lee, Tae-Soo (Department of Biology, University of Incheon)
Publication Information
Journal of Life Science / v.20, no.4, 2010 , pp. 623-631 More about this Journal
Abstract
Hericium erinaceus, an edible and medicinal mushroom belonging to the Basidiomycota family, has been used for curing gastric ulcers and stomach cancers in human beings and is also known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma in mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to as Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from the fruiting body of the mushroom. In in vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cell lines such as Sarcoma 180, HepG2, HT-29 and NIH3T3 at concentrations of $10{\sim}2,000\;{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited a life prolongation effect of 29.1~54.1% in mice previously inoculated with Sarcoma 180. Fr. Na increased the numbers of spleen cells by 2.9 fold at a concentration of $50\;{\mu}g/ml$ compared with the control. Fr. Na improved the immuno-potentiating activity of B lymphocytes by increasing alkaline phosphatase activity by 5.5 fold compared with the control at a concentration of $200\;{\mu}g/ml$. Fr. NaCl increased the numbers of peritoneal exudate cells and circulating leukocytes by 4 and 2.3 folds at a concentration of 50 mg/kg, respectively. Therefore, the crude polysaccharides extracted from the fruiting body of H. erinaceus could improve antitumor activities in mice.
Keywords
Antitumor effect; crude polysaccharides; immunomodulation; Hericium erinaceus;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Jin, M. L. 1996. A study on the activation of immune cell and transcription factor of pine nut mushroom. Ph. D thesis of Seoul National University Graduate School. pp. 1-121.
2 Kawakishi, H., A. Shimada, S. Hosokawa, and H. Mori. 1996. Erinacenes, E, F, G, stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceus. Tetrahedron Lett. 37, 7399-7402.   DOI
3 Kim. B. K., E. K. Park, and M. J. Shim. 1979. Studies on constituents of higher fungi of Korea (XXIV), antineoplastic activities of Coriolus versicolor (Fr.). Quel, Pleurotus ostreatus (Fr.) Kummer and Lentnus edodes (Berk.) Sing. Arch. Pharm. Res. 2, 145-149.   DOI
4 Kim, S. B., K. W. Lee, H. Y. Kim, M. J. Shim, H. S. Rho, H. S. Lee, M. W. Lee, W. Y. Lee, and T. S. Lee. 2006. Inhibitive effect of mouse Sarcoma 180 by crude polysaccharides extracted from fruiting Body of Armillaria mellea. Kor. J. Mycol. 34, 98-104.   DOI
5 Kim, S. B., K. W. Lee, W. Y. Lee, and T. S. Lee. 2007. Studies on immuno-modulatory and antitumor effects of crude polysaccharides extracted from fruiting Body of Oudemansiella radicata. Kor. J. Mycol. 35, 109-114.   과학기술학회마을   DOI
6 Lee, G. W., H. Y. Kim, W. H. Lee, and T. S. Lee. 2007. Antitumor and immuno-modulatory effect against mouse sarcoma 180 of crude polysaccharides extracted from fruiting Body of Armillaria tabescens. Kor. J. Myco. 35, 101-108.   과학기술학회마을   DOI
7 Lee, G. W., H. Y. Kim, H. Hur, M. W. Lee, M. J. Shim, M. Y. Lee, and T. S. Lee. 2008. Antitumor and immuno-modulatory effect of crude polysaccharides from fruiting body of Tremella aurantialba against mouse Sarcoma 180. Kor. J. Mycol. 36, 66-74.   과학기술학회마을   DOI
8 Allison, D. C. and P. Ridolpho. 1980. Use of a trypan blue assay to measure the deoxyribonucleic acid content and radioactive labeling of viable cells. J. Histochem. Cytochem. 28, 700-703.   DOI   ScienceOn
9 Mizno, T. 1992. Antitumor active polysaccharides isolated from the fruiting body of Hericium erinaceus, an edible and medicinal mushroom called yamabushitake or houtou. Biosci. Biochem. 56, 347-348   DOI
10 Ying, J. Z., X. L. Mao, Q. M. Ma, Y. C. Zong, and H. A. Wen. 1987. Icons of medical fungi from China. Science Press, Beijing, China.
11 Mossman, B. T. 1983. In vitro approaches for determining mechanism of toxicity and carcinogenicity by asbestos in the gastrointestinal and respiratory tracts. Environ. Health Perspect. 53, 155-161.   DOI
12 Oh, Y. H., W. Y. Lee, M. W. Lee, M. J. Shim, and T. S. Lee. 2004. Immuno-modulatory and antitumor effect of crude polysaccharides extracted from sclerotium of Grifola umbellata. Kor. J. Mycol. 32, 23-30.   DOI
13 Oh, Y. H., S. B. Kim, K. W. Lee, H. Y. Kim, M. J. Shim, H. S. Rho, H. S. Lee, M. W. Lee, W. Y. Lee, and T. S. Lee. 2006. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from Tremella fuciformis. Kor. J. Mycol. 34, 105-111.   과학기술학회마을   DOI
14 Ohno, N., Y. Arai, I. Suzuki, and T. Yadomae. 1986. Induction of alkaline phosphatase activity in murine spleen cells treated with various mitogens. J. Pharmacobio-Dyn. 9. 593-599.   DOI
15 Park, W. H. and H. D. Lee. 2002. Mushrooms in Korea. Kyohak publishing Co., Ltd., Seoul.
16 Shim, M. J. 1980. Stimulating effects of Coriolus versicolor constituents on immune response. Kor. J. Mycol. 8, 115-116
17 Shim, S. M., K. H. Im, W. Y. Lee, J. W. Kim, M. J. Shim, M. W. Lee, and T. S. Lee. 2003. Studies on immuno-modulatory and antitmor effects of crude polysaccharides extracted from Paecilomyces sinclairii. Kor. J. Mycol. 31, 155-160.   DOI
18 Shim, S. M., K. W. Im, J. W. Kim, W. Y. Lee, H. W. Kim, M. W. Lee, and T. S. Lee. 2003. The immuno-modulatory and antitumor effects of crude polysaccharides extracted from Daedaleopsis tricolor. Kor. J. Mycol. 31, 161-167.   DOI
19 Arthur, C. and M. D. Guyton. 1986. Textbook of medical physiology. 7th eds. W. B. Saunder Company. pp. 51-59.
20 Lee, K. Y., M. J. Han, S. Y. Park, and D. H. Kim. 2000. In vitro and in vivo antitumor activity of the fruit body of Phellinus linteus. Kor. J. Food Sci. Technol. 32, 477-480.
21 Chihara, G., Y. Maeda, G. Hamuro, Y. Arai, and F. Fukuoka. 1969. Inhibition of mouse Sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) Sing. Nature 222, 687-688.   DOI
22 Cho, S. M., J. H. Lee, S. B. Han, H. M. Kim, S. H. Yoo, and I. D. Yoo. 1995. Immuno-stimulating polysaccharides from the fruiting bodies of Fomitella fraxinea (1)-Characterization of polysaccharides extracted with neutral sodium chloride solution. Kor. J. Mycol. 23, 332-339.
23 Cho, S. M., J. H. Lee, S. B. Han, H. M. Kim, S. H. Yoo, and I. D. Yoo. 1995. Immuno-stimulating polysaccharides from the fruiting bodies of Fomitella fraxinea (2)-Isolation and characterization of hot-water extracted with polysaccharides. Kor. J. Mycol. 23, 340-347.
24 Choi, Y. I., K. W. Lee, H. Hur, W. Y. Lee, and T. S. Lee. 2008. Immuno-potentiating and antitumor effects against mouse Sarcoma 180 by crude polysaccharides extacted from fruiting body of Russula rosacea. Kor. J. Mycol. 36, 84-92.   DOI
25 Deonizot, F. and R. Lang. 1986. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89, 271-277.   DOI
26 Fukuda, K., T. Uematsu, A. Hamada, and S. Akiya. 1975. The polysaccharide from Lampteromyces Japonicus. Chem. Pharm. Bull. 23, 1955-1961.   DOI   ScienceOn
27 Ji, I. H. and M. N. Kim. 2000. Antimutagenic and cytotxicity effects of Phellinus linteus extracts. J. Kor. Soc. Food Sci. Nutr. 29, 322-330.
28 Hur, H., Y. I., Choi, and T. S. Lee. 2008. Antitumor and immuno-potentiating activity against mouse Sarcoma 180 by crude polysaccharides extracted from fruiting body of Tricholoma matsutake. J. Life Sci. 18, 1290-1298.   DOI