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Efficient virus elimination for apple dwarfing rootstock M.9 and M.26 via thermotherapy, ribavirin and apical meristem culture (사과 왜성대목 M.9 및 M.26의 고온, ribavirin, 생장점 배양을 통한 바이러스 제거)

  • Kwon, Young Hee;Lee, Joung Kwan;Kim, Hee Kyu;Kim, Kyung Ok;Park, Jae Seong;Huh, Yoon Sun;Park, Eui Kwang;Yoon, Yeo Joong
    • Journal of Plant Biotechnology
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    • v.46 no.3
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    • pp.228-235
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    • 2019
  • Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy ($37^{\circ}C$, 6 weeks) and chemotherapy($Ribavirin^{(R)}$). The detection of apple viruses was accomplished by Enzyme-linked Immuno-Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT- PCR method was 10 ~ 30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30 ~ 40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.

Analysis of Seabottom and Habitat Environment Characteristics based on Detailed Bathymetry in the Northern Shore of the East Sea(Gyeongpo Beach, Gangneung) (정밀 해저지형 자료 기반 동해 북부 연안(강릉 경포) 서식지 해저면 환경 특성 연구)

  • Lee, Myoung Hoon;Rho, Hyun Soo;Lee, Hee Gab;Park, Chan Hong;Kim, Chang Hwan
    • Economic and Environmental Geology
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    • v.53 no.6
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    • pp.729-742
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    • 2020
  • In this study, we analyze seabottom conditions and characteristics integrated with topographic data, seafloor mosaic, underwater images and orthophoto(drone) of soft-hard bottom area around the Sib-Ri rock in the northern shore of the East Sea(Gyeongpo Beach, Gangneung). We obtained field survey data around the Sib-Ri rock(about 600 m × 600 m). The Sib-Ri rock is formed by two exposed rocks and surrounding reef. The artificial reef zone made by about 200 ~ 300 structures is shown the western area of the Sib-Ri rock. The underwater rock region is extended from the southwestern area of the exposed the Sib-Ri rock with 9 ~ 11 m depth range. The most broad rocky seabottom area is located in the southwestren area of the Sib-Ri rock with 10 ~ 13 m depth range. The study area were classified into 4 types of seabottom environment based on the analysis of bathymetric data, seafloor mosaics, composition of sediments and images(underwater and drone). The underwater rock zones(Type I) are the most distributed area around the Sib-Ri Rock(about 600 m × 600 m). The soft seabottom area made by sediments layer showed 2 types(Type II: gS(gravelly Sand), Type III: S(Sand)) in the areas between underwater rock zones and western part of the Sib-Ri rock(toward Gyeongpo Beach). The artificial reef zone with a lot of structures is located in the western part of the Sib-Ri rock. Marine algae(about 6 species), Phylum porifera(about 2 species), Phylum echinodermata(about 3 species), Phylum mollusca(about 3 species) and Phylum chordata(about 2 species) are dominant faunal group of underwater image analysis area(about 10 m × 10 m) in the northwestern part of the Sib-Ri rock. The habitat of Phylym mollusca(Lottia dorsuosa, Septifer virgatus) and Phylum arthropoda(Pollicipes mitella, Chthamalus challengeri hoek) appears in the intertidal zone of the Sib-Ri rock. And it is possible to estimate the range and distribution of the habitat based on the integrated study of orthphoto(drone) and bathymetry data. The integrated visualization and mapping techniques using seafloor mosaic images, sediments analysis, underwater images, orthophoto(drone) and topographic data can provide and contribute to figure out the seabottom conditions and characteristics in the shore of the East Sea.

Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea (메타전사체 분석을 이용한 국내 대추나무의 바이러스 감염실태)

  • Hong-Kyu Lee;Seongju Han;Sangmin Bak;Minseok Kim;Jean Geung Min;Hak ju Kim;Dong Hyun Kang;Minhui Kim;Wonyoung Jeong;Seungbin Baek;Minjoo Yang;Taegun Lim;Chanhoon An;Tae-Dong Kim;Chung Youl Park;Jae Sun Moon;Su-Heon Lee
    • Research in Plant Disease
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    • v.29 no.3
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    • pp.276-285
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    • 2023
  • This work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain reaction analysis, and nucleotide sequence analysis. These analyses identified the presence of two DNA viruses, jujube-associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV), and one RNA virus, jujube yellow mottle-associated virus (JYMaV). All samples collected were confirmed to be infected by at least one of the three viruses, with most showed multiple infections. The detection rates of JuBV, JYMaV, and JuMaV were 100%, 90.2%, and 8.2%, respectively. Only three combinations of viral infections were found: 9.8% of samples showed single infection of JuBV, 82.0% showed double infection of JuBV+JYMaV, and 8.2% showed triple infection of JuBV+JYMaV+JuMaV. Sequence analysis of the three viruses showed very high homology with respective virus isolates reported in China. This study is predicted to provide fundamental data to produce virus-free jujube seedlings and represents the first report of JuBV and JuMaV infection in Korea.

Diagenetic History of the Ordovician Chongson Limestone in the Chongson Area, Kangwon Province, Korea (강원도 정선 지역 오르도비스기 정선석회암의 속성 역사)

  • Bong, Lyon-Sik;Chung, Gong-Soo
    • Journal of the Korean earth science society
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    • v.21 no.4
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    • pp.449-468
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    • 2000
  • The Ordovician Chongson Limestone deposited in the carbonate ramp to the rimmed shelf shows diverse diagenetic features. The marine diagenetic feature appears as isopachous cements surrounding ooids and peloids. Meteoric diagenetic features are recrystallized finely and coarsely crystalline calcite, evaporite casts filled with calcite, and isopachous sparry calcite surrounding ooid grains. Shallow burial diagenetic features include wispy seam, microstylolite, and dissolution seam whereas deep burial features include stylolite, burial cements. blocky calcite with twin lamellae, and poikilotopic calcite. Dolomites consist of very finely to finely crystalline mosaic dolomite formed as supratidal dolomite, disseminated dolomite of diverse origin, patchy dolomite formed from bioturbated mottles, and saddle dolomite of burial origin. Silicified features include calcite-replacing quartz and fracture-filling megaquartz. Burial cements characterized by poikilotopic texture show ${\delta}^{18}$O value of -10.4 %$_o$ PDB, ${\delta}^{13}$C value of -1.0%$_o$ PDB and 504ppm Sr, 3643ppm Fe, and 152ppm Mn concentrations. Finely and coarsely crystalline limestones show similar ${\delta}^{18}$O and ${\delta}^{13}$C value to those of burial cements; however, they show lower Sr and higher Fe and Mn concentrations than burial cements. This suggests that very finely and coarsely crystalline limestones were recrystallized in freshwater and then they were readjusted geochemically in the burial setting whereas the burial cements were formed in relatively high temperature and low water/rock ratio conditions. Very finely and finely crystalline mosaic dolomites with ${\delta}^{18}$O value of -8.2%$_o$ PDB, ${\delta}^{13}$C value of -1.9 %$_o$ PDB, and 213ppm Sr, 3654ppm Fe, and 114ppm Mn concentrations, respectively are interpreted to have been formed penecontemporaneously in supratidal flat and then recrystallized in the low water/rock ratio burial environment. Geochemical data suggest that the low water/rock ratio burial environment was the dominant diagenetic setting in the Chongson Limestone. The Chongson Limestone has experienced marine and meteoric diagenesis during early diagenesis. With deposition of Haengmae and Hoedongri formations part of the Chongson Limestone was buried beneath these formations and it experienced shallow burial diagenesis. During the Devonian the Chongson Limestone was tectonically deformed and subaerially exposed. During the Carboniferous to the Permian about 3.3km thick Pyongan Supergroup was deposited on the Chongson Limestone and the Chongson Limestone was in deep burial depths and stylolite, burial cements, blocky calcite and saddle dolomite were formed. After this burial event the Chongson Limestone was subaerially exposed during the Mesozoic and Cenozoic by three periods of tectonic disturbance including Songnim, Daebo and Bulguksa disturbance. Since the Bulguksa disturbance during Cretaceous and early Tertiary the Chongson Limestone has been subaerially exposed.

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Reading "Money": Value and Signification of Money (화폐 읽기: 화폐의 의미작용과 가치)

  • Won, Yong-Jin;Hong, Sung-Il
    • Korean journal of communication and information
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    • v.41
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    • pp.75-107
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    • 2008
  • The paper aims to figure out the meaning of money in terms of communication in which we can find the specific communicative and cultural form of money. In the modern capitalist society, we cannot limit money within the economical terms - for example, store of value, medium of exchange, unit of account and means of payment - because money mediates all human activities beyond the economic boundary and conveys specific meaning in the social and the cultural area. Money can be the medium of the cultural and the communicational as well as the medium of the economic. In this respect, we've try to articulate money with linguistic or semiotic insight. Through this theoretical dialogue, we find two significations of money as a medium of communication. The first signification is meta-signification which drives the individual to the unlimited accumulation of the money. Meta-signification displace the second signification of the money that is the singular, over-determined and the mosaic significations. In this process money can be the signifier without signification. And then, money is the Master signifier which all sign should be identified imaginary. Finally, Money is not only the re-presense (Darstellung) of all sings but also the representative (Vertretung) of all signs. But this double position creates some tensions and makes master signifier of money unstable. Man's analysis of Bonapartism, which shares the linguistic or semiotic insight, shows the crack of the re-presence between the representative. Like Marx's analysis, the money has the tension between two signification which makes the room for the struggle to signify.

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Populations of Rod and Cone Photoreceptors in the Hamster Retina (햄스터 망막에서의 광수용체 분포)

  • Yu, Song-Hee;Kim, Hyun-Jin;Lee, Kyoung-Pil;Lee, Eun-Shil;Lee, Jea-Young;Jeon, Chang-Jin
    • Applied Microscopy
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    • v.39 no.4
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    • pp.291-299
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    • 2009
  • We report on a quantitative analysis of cone and rod photoreceptors in hamster retina. Cone and rod photoreceptors were counted in retinal whole mounts using differential interference contrast (DIC) optics microscopy after staining of cone photoreceptors were stained with peroxidase-labeled peanut lectin. Middle-to-long-wave-sensitive-(M/L-), and shortwave-sensitive-(S-) cone opsins were visualized by observed using confocal microscope after immunocytochemical procedure. The average cone density was 9,307 $cells/mm^2$, giving a total of cones of 293,060 cone cells per retina. The peak density of cone cells (12,857 $cells/mm^2$) was found 0.3 mm from the optic disk (OD) of the nasal retina. The average rod density was 300,082 $cells/mm^2$, giving a total number of rods of 9,448,150 cells. The peak density of rod cells was found 0.3 mm from the OD of the dorsal retina. Of all photoreceptors studied, the total percentage of rods and cones were 96.99% and cones 3.01%, respectively. The mean ratio of rod and cone was 32.24 : 1. The cone photoreceptors of hamster contained both M/L- and S-cone opsins. The present results suggest that the hamster retina is strongly rod-dominated with some photopic property of vision.

Studies on the life history of cotton aphid, Aphis gossypii Glover (Homoptera) (목화진딧물(Aphis gossypii Glover)의 생활사에 관한 연구)

  • Shim J.Y;Park J.S.;Paik W.H.
    • Korean journal of applied entomology
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    • v.18 no.2 s.39
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    • pp.85-88
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    • 1979
  • The cotton aphid, Aphis gossypii Glover, is known as the most important vectant vector of citrus tristeza virus, cucumber mosaic virus, potato virus Y and potato leafroll virus. This study conducted to investigate the life history of cotton aphid at Suweon, Korea$(Lat.37^{\circ}16'N,\;Long\;126^{\circ}59'E)$. The aphids were reared in small cages placed over twig of hibiscus and on the leaves of cucumber. The results obtained were summarized as follows: 1. Overwintering eggs hatched from mid to late April, with a hatching rate averaging seventy-nine percent. 2. The early-born progeny have 22 generations and the late-born progeny have 6 generations on hibiscus and cucumber from April to October. 3. The fundatrigeniae leave tile the primary host in late May to early June and migrate to the secondary hosts. 4. From early to mid Oct., the gynoparae migrate from the secondary hosts to the primary host. 5. The average length of life was about 29 days and they produced an average of 70 nymphs each. 6. The maximum number of aphids produced per female was 117 in the spring. 7. The developmental period ranged from 6 to 16 days (average 8 days), the reproductive period from 12.2 to 24.6 days (average 19 days). 8. The average number of nymphs produced by a female per dys was about 3.7, with a maximum number of 17.

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The Structural and Functional Analysis of Landscape Changes in Daegu Metropolitan Sphere using Landscape Indices & Ecosystem Service Value (경관지수와 생태계용역가치를 활용한 대구광역도시권 경관의 구조적·기능적 변화 분석)

  • Choi, Won-Young;Jung, Sung-Gwan;Oh, Jeong-Hak;You, Ju-Han
    • Journal of the Korean Association of Geographic Information Studies
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    • v.8 no.4
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    • pp.102-113
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    • 2005
  • Ecosystem is composed of human, biotic and abiotic environment. Landscape is an ecosystem which appear in a unit region. These landscape are the spatiotemporal land mosaic which is combined with various landscape elements. And, land use and land cover changes are important factors of landscape structure changes. This study is mainly focused on the analysing the spatiotemporal change patterns of Daegu metropolitan sphere forest landscape, using landscape indices and Ecosystem Service Value (ESV) which quantify ecosystem structures and functions. The results of this study are as follow: The encroachment and fragmentation of forest were due to linear developments, i. e. road construction, rather than large-scale developments such as residental lands or industrial complexes. And, the core area percentages of landscape gradually decreased and these could possibly deteriorate the soundness of forest areas by reducing the core areas which are habitats of species. In addition, there was intimate relations between ESV and forest landscape area. The results of this study can be detached standards for impartial judgements between the logic of development & conservation, and basic standards for the establishment of development plans, i. e. metropolitan-plans, which are adequately reflected ecosystem value.

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Improvement of KOMPSAT Imagery Locational Accuracy Using Value-Added Processing System (부가처리시스템을 이용한 다목적실용위성 영상자료 위치정확도 개선)

  • LEE, Kwang-Jae;YUN, Hee-Cheon;KIM, Youn-Soo
    • Journal of the Korean Association of Geographic Information Studies
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    • v.18 no.4
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    • pp.68-80
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    • 2015
  • To increase the utilization of the KOrea Multi-Purpose SATellite(KOMPSAT) series imagery being developed pursuant to the national space development program, high quality images with enhanced locational accuracy should be created through standardized post-processing processes. In the present study, using the Value-Added Processing System(VAPS) constructed for the post-processing of KOMPSAT imagery, location correction experiments were conducted using KOMPSAT-2 and -3 imagery from domestic and overseas regions. First, 50 pieces from each of KOMPSAT-2 imagery were selected from South Korean and North Korean regions, and modeling was conducted using GCP Chips. According to the results, the Root Mean Square Errors(RMSE) for South Korea and North Korea were 1.59 pixels and 2.04 pixels, respectively, and the locational accuracy of ortho mosaic imagery using check points were 1.33m(RMSE) and 1.90m(RMSE), respectively. Meanwhile, in the case of overseas regions for which GCP could not be easily obtained, the improvement of locational accuracy could be identified through image corrections using Open Street Map(OSM). The VAPS and reference materials used in the present study are expected to be very useful in constructing a precise image DB for entire global regions.

Monitoring Method Using Moving CCTV in Common Duct (이동형 CCTV 장치를 이용한 공동구 모니터링 방법)

  • Kang, Jin-A;Kim, Tae-Hoon;Oh, Yoon-Seuk;Choi, Hyun-Sang
    • Journal of the Korean Association of Geographic Information Studies
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    • v.14 no.4
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    • pp.1-12
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    • 2011
  • There has been the increasing concern with the safety of seven major urban infrastructure such as road, electricity, water supply, sewerage and so on due to urban expansion and new town development. However, high technology development for the common duct which can be an alternative for the safety issue has not been completed due to the law of the national security area. Existing management method of the common duct by people could not respond to the urgent accidents adequately and immediately since it is impossible for us to get access to that in case of fire or gas leak. This study suggests to the method of installing monitoring devices and processing CCTV images with a water supply in a TestLab(a variety of the USN(Ubiquitous Sensor Network) equipment was tested in the TestLab in KICT). The suggested management method of common duct facilities make it possible to do real-time monitoring and prompt access and response to an accident inside the common duct.