• Title/Summary/Keyword: morphometric analyses

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Morphological and molecular characterization of the genus Coolia (Dinophyceae) from Bahía de La Paz, southwest Gulf of California

  • Morquecho, Lourdes;Garate-Lizarraga, Ismael;Gu, Haifeng
    • ALGAE
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    • v.37 no.3
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    • pp.185-204
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    • 2022
  • The genus Coolia A. Meunier 1919 has a global distribution and is a common member of epiphytic dinoflagellate assemblages in neritic ecosystems. Coolia monotis is the type species of the genus and was the only known species for 76 years. Over the past few decades, molecular characterization has unveiled two species complexes that group morphologically very similar species, so their limits are often unclear. To provide new knowledge on the biogeography and species composition of the genus Coolia, 16 strains were isolated from Bahía de La Paz, Gulf of California. The species were identified by applying morphological and molecular approaches. The morphometric characteristics of all isolated Coolia species were consistent with the original taxa descriptions. Phylogenetic analyses (large subunit [LSU] rDNA D1 / D2 and internal transcribed spacer [ITS] 1 / 5.8S / ITS2) revealed a species assemblage comprising Coolia malayensis, C. palmyrensis, C. tropicalis, and the C. cf. canariensis lineage. This is the first report of Coolia palmyrensis and C. cf. canariensis in Mexico and C. tropicalis in the Gulf of California. Our results strengthen the biogeographical understanding of these potentially harmful epiphytic dinoflagellate species.

Ventral Anterior Cingulate Atrophy as a Predisposing Factor for Transient Global Amnesia

  • Jeewon Suh;Young Ho Park;Hang-Rai Kim;Jae-Won Jang;SangHak Yi;Min Ju Kang;Yun Jung Bae;Byung Se Choi ;Jae Hyoung Kim;SangYun Kim
    • Dementia and Neurocognitive Disorders
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    • v.23 no.2
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    • pp.89-94
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    • 2024
  • Background and Purpose: This study aimed to evaluate the brain magnetic resonance imaging (MRI) of patients with acute transient global amnesia (TGA) using volumetric analysis to verify whether the brains of TGA patients have pre-existing structural abnormalities. Methods: We evaluated the brain MRI data from 87 TGA patients and 20 age- and sex- matched control subjects. We included brain MRIs obtained from TGA patients within 72 hours of symptom onset to verify the pre-existence of structural change. For voxel-based morphometric analyses, statistical parametric mapping was employed to analyze the structural differences between patients with TGA and control subjects. Results: TGA patients exhibited significant volume reductions in the bilateral ventral anterior cingulate cortices (corrected p<0.05). Conclusions: TGA patients might have pre-existing structural changes in bilateral ventral anterior cingulate cortices prior to TGA attacks.

Geographic Variation and Interspecific Hybridization between Two Species of the Genus Agkistrodon (Crotalidae) in Korea (한국산 살모사속(뱀과) 2종의 지리적 변이 및 종간 잡종)

  • 백남극;양서영
    • Animal Systematics, Evolution and Diversity
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    • v.5 no.1
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    • pp.77-88
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    • 1989
  • Genetic and morphometric comparison between two species of the genus Agkistrodon were performed to estimate the degree of genic variation and to clarify the taxonomic status of Cheju population, a suspected hybrid form between A. blomhoffii brevicaudus and A u ussuriensis. A sum of 147 specimens representing six populations of A.b. brevicaudus and two populations of A ussuriensis was used in this study. Out of the 21 loci examined, 5 loci (Adh, Ldh-2, Mdh-2, Mpi, Pgi) were monomorphic with identical mobility in both species. O Other Sioci (Aco, Gp, Fum, [po and Xdh in A b. brevicaudus; Est, Got-l, Gp, Mdh-l and 6Pgd in A ussuriensis) were highly poplymorphic. The degree of genic variation of Ab. b brevicaudus and A ussuriensis was A=1.SS, P=42.1%, HD=0.096, HG=O.l1S, and A= 1.4S, P = 31.6%, HD=0.117, HG =0.121, repectively. These values are twofold higher t than those of other reptilian species reported (Selander, 1976) including Korean species of Rhabdophis tigrina and Elaphe dione (Paik and Yang, 1986, 1987). The average values of t the genetic similarities among six populations of A b. brevicaudus and two populations of A ussuriensis were S=0.919 and S=0.962, respectively, whereas the value between species was S = 0.662. Presumed divergent time estimate (Nei, 1975) of these two species was about 1 1.8 million years ago. The analysis of the geographic variations of various morphological c characters was based on Gloyd’s criteria (Gloyd, 1972). Coloration of tongue and tail tip, t the number of ventral scales, and the number of subcaudal scales are good diagnostic c characters to identify these two species. The pattern of cross band is, however, highly variable within and between populations of both species. No hybrid was detected and the s suspected hybrid form of Cheju population falls within the range of A. ussuriensis in the g genetic and morphometric analyses.

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Morphometric and Ultrastructural Change of Myelin-Associated Glycoprotein (MAG)-Immunoreactive Oligodendrocytes by Aging (노화에 의한 Myelin-Associated Glycoprotein (MAG) 면역반응 희소돌기아교세포의 형태계측학적 및 미세구조적 변화)

  • Cho, Ik-Hyun;Park, Chang-Hyun;Lee, Jong-Hwan;Bae, Chun-Sik;Ye, Sang-Kyu;Lee, Beob-Yi;Park, Seung-Hwa;Koh, Ki-Seok;Kim, Jin-Suk;Chang, Byung-Joon
    • Applied Microscopy
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    • v.36 no.2
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    • pp.119-130
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    • 2006
  • To investigate the role of myelin-associated glycoprotein (MAG) in the normal aging process, aging-related morphometric and ultrastructural analyses of the MAG-positive (MAG-(+)) oligodendrocytes were carried out in the cerebral cortex of the Sprague-Dawley rats. In the aged rats, the density of MAG-(+) oligodendrocytes was significantly decreased in the cortical layer (IV-VI) compared with that of the adult rats. However, the percentage of medium and dark types of oligodendrocytes was significantly increased by aging. In the aged rats, the mean nuclear area of the MAG-(-) oligodendrocytes was interestingly reduced compared with that of MAG-(+) oligodendrocytes. In addition, MAG immunoreactive products were markedly decreased in the medium-dark type of oligodendroglial cytoplasm and processes, and were scarcely localized in the dark type of oligodendrocytes of the aged rats. These results suggest that degeneration of oligodendrocytes-myelin system by aging is associated with down regulation of MAG, and that may contribute to further understanding of the biology of MAG in the oligodendrocytes-myelin system.

Morphometric Analyses of Damaster(Coptolabrus) jankowskii from Korea(Coleoptera : Carabidae) (한국산(韓國産) 멋쟁이딱정벌레의 계량형태학적(計量形態學的) 분석(分析) (초시목(鞘翅目) : 딱정벌레과(科)))

  • Kwon, Yong Jung;Park, Jong Kyun
    • Current Research on Agriculture and Life Sciences
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    • v.7
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    • pp.127-151
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    • 1989
  • The ground beetles or carabids are essentially predaceous feeding on a wide variety of insects including forest pests, slugs and land snails containing those injurious to livestock or veterinary, thus many are predominantly beneficial and serve as natural enemies. In the present investigation, some morphometric multivariate analysis were done for 9 different populations in 5 subspecies of D. (C.) jankowskii, which are one of the most common ground beetles in Korea As the results, when the comparison was conducted between intraspecific groups regardless of subspecies in external morphological characters, the average group membership revealed 97.46% correct assignment For intersubspecific comparisons alone 96.3% were correctly classified. Between the groups of ssp. jankowskii an average of 100% individuals were classified in their known group. Thus the predict group membership was highly significant(P<0.001), exceeding so-called 'the 75% rule'. Whereas, the average group membership using the male genitalic characters represented less than the 75% assignment, except only in ssp. quelpartianus (85.6%). The population from Is. Chindo were described here as a new subspecies for qualitative as well as the resultant quantitative differences. Therefore, a total of 7 subspecies are represented in Korea. Among them, the nominate subspecies, ssp. jankowskii(sensu lato), revealed distinct intrasubspecific differences between different geographic populations. These differences can be as notable as intersubspecific variation which need substantial revision of the previous intuitional concepts on the infraspecific level.

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Systematic Studies of the Genus Cobitis (Pisces: Cobitidae) in Korea I. Geographic Variations and classification of Cobitis koreensis (한국산 Cobitis속 (Pisces: Cobitidae) 어류의 계통분류학적 연구 1. 참종개(Cobitis kireensis)의 지리적 변이 및 분류에 관하여)

  • 양서영;박병상;김재야
    • The Korean Journal of Zoology
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    • v.32 no.3
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    • pp.242-251
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    • 1989
  • Morphometric and genetic analyses on six populations of the two subspecies of Cobitis koreensis were performed to investigate the geographic variation and to clarify their taxonomic status. No significant differences between subspecies were found. Puan population of C. k. pumilus tends to be smaller in numbers of cross bars and dorsal spots, than those of C. k. koreensis populations with no statistical significance. Average degree of genic variation among six populations of the two subspecies was A=1.5, P =37.3%, HD=0.053, and HG=0.097. Marginal populations of Samchog (C. k. koreensis) and Puan(C. k. pumilus) were least variable: A= 1.3, P=23.8%, HD=0.0l0, HG=0.043, and A= 1.2, P=19.0%, HD=0.029, HG=0.078 respectively.The difference between HD and HG in all populations may indicate a patchness distribution between demes within each population. Genetic similarities between the two subspecies were, on the average, S=0.894, and this value correspond to the average genetic similarities among five populations of C. k. koreensis (5=0.899). Chongup population of C. k. koreensis and Puan population of C. k. pumilus was most similar genetically (5 =0.931). The results of morphometric and genetic analysis in this study indicate that C. k. pumilus is not a valid subspecies and should be considered as a population of C. koreensis.

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Numerical taxonomic study of the genus Sorbaria (Ser.) A. Braun in Asch. (Rosaceae) (쉬땅나무속(장미과)의 수리분류학적 연구)

  • SONG, Jun-Ho;HONG, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.48 no.3
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    • pp.230-247
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    • 2018
  • We conducted principal component analyses using the quantitative characteristics of the genus Sorbaria to investigate and explore morphological variation and diagnostic characteristics. The genus Sorbaria was divided into two groups based on erect or pendulous inflorescence, the existence of hairs on the ovary and follicle surfaces, the number of stamens, and the shape of the sepal. As a result of our investigation and of a morphometric analysis, these two groups could be also classified using quantitative characteristics, in this case the number of leaflets, the size of the leaflets, the width of the inflorescence, the size of the sepal, the petal, and the follicles and seeds. In the Sorbifolia group (S. grandiflora and S. sorbifolia complex), the size of lateral leaflets, number of veins, gland and stellate density on the abaxial surface of leaflets, and the petal and follicle size were found to be useful identification characteristics. The terminal and lateral leaflet size and the gland and stellate density on the abaxial surface of the leaflets were found to be characters of taxonomic value for the Kirilowii group (S. arborea complex, S. kirilowii, and S. tomentosa complex). The results of the numerical analysis conducted here can provide valuable information to those reconsidering and delimiting a taxonomic revision of the genus Sorbaria.

(-)-Epigallocatechin-3-gallate Modulates the Differential Expression of Survivin Splice Variants and Protects Spermatogenesis During Testicular Torsion

  • Al-Ajmi, Nada;Al-Maghrebi, May;Renno, Waleed Mohammed
    • The Korean Journal of Physiology and Pharmacology
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    • v.17 no.4
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    • pp.259-265
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    • 2013
  • The anti-apoptotic effect of (-)-epigallocatechin-3-gallate (EGCG) during unilateral testicular torsion and detorsion (TT/D) was established in our previous study. In mice, the smallest inhibitor of apoptosis, survivin, is alternatively spliced into three variants, each suggested to have a unique function. Here, we assessed how EGCG exerts its protective effect through the expression of the different survivin splice variants and determined its effect on the morphology of the seminiferous tubules during TT/D. Three mouse groups were used: sham, TT/D+vehicle and TT/D treated with EGCG. The expression of the survivin variants (140 and 40) and other apoptosis genes (p53, Bax and Bcl-2) was measured with semi-quantitative RT-PCR. Histological analysis was performed to assess DNA fragmentation, damage to spermatogenesis and morphometric changes in the seminiferous tubules. In the TT/D+vehicle group, survivin 140 expression was markedly decreased, whereas survivin 40 expression was not significantly different. In parallel, there was an increase in the mRNA level of p53 and the Bax to Bcl-2 ratio in support of apoptosis induction. Histological analyses revealed increased DNA fragmentation and increased damage to spermatogenesis associated with decreased seminiferous tubular diameter and decreased germinal epithelial cell thickness in the TT/D+vehicle group. These changes were reversed to almost sham levels upon EGCG treatment. Our data indicate that EGCG protects the testis from TT/D-induced damage by protecting the morphology of the seminiferous tubules and modulating survivin 140 expression.

Taxonomic Stautus of Striped Field Mice(Mammalia: Rodenita) from Wando Island, Korea (한국 완도에 서식하고 있는 등줄쥐(포유강: 설치목)의 분류학적 위치)

  • Hung Sun Koh;Bo Yeong Lee;Yong Kim Ki Kim;Sang Kyu Yoo;Byeong Kug Yang
    • Animal Systematics, Evolution and Diversity
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    • v.14 no.1
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    • pp.51-57
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    • 1998
  • Thirty one morphometric characters of striped field mice(Apodemus agrarius) from eight localities in Korea were analyzed by multivariate methods in order to confirm taxonomic status of specimens from Wando island. Two subgroups were recognized: a small-size form from six localities (Mt. Taebaek, Mt. Weolak, Cheongju, Mt. Chiri, Kunsan, and Jin isoland) and a large-size form from Wando island and Cheju island. It is confirmed that the small-size form from six localities is A. a. coreae, as described by Thomas(1908), and that the large-size form from Cheju island is A. a. chejuensis, as named by Johnson and Jones (1955). Furthermore, it is confirmed that large-size specimens from Wando island is and Cheju island. It is confirmed that the small-size form from six localities is A. a coreae, as described by Thomas(1908), and that the large-size form from Cheju island is A. a. chejuensis, as named by Johnson and Jones(1955). Furthermore, it is confirmed that large-size specimens from Wando island is subspecies A. a. chejuensis, as suggested by koh (1989). In future, analyses with specimens from islands in southern coasts are necessary to clarify the taxonomic status of Apodemus agrarius in Korea.

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Thorea indica sp. nov. (Thoreales, Rhodophyta) from Uttar Pradesh, India

  • Necchi, Orlando Jr;Paiano, Monica O.;West, John A.;Ganesan, E. K.;Goer, Susan Loiseaux-de
    • ALGAE
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    • v.30 no.4
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    • pp.265-274
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    • 2015
  • Thorea indica sp. nov. is described from the Sai River, Uttar Pradesh, India (26°39′00.7″ N, 80°47′38.3″ E). Its classification is based on molecular sequences of the plastid-encoded RuBisCO large-subunit gene, rbcL and the barcode region of the mitochondrial encoded cytochrome c oxidase subunit 1, cox1, and morphological data. The sequence analyses confirm a new species of Thorea. The cox1 barcode sequence had 90.4-90.8% identity with Thorea sp. from Australia and Thorea hispida from Hawaii and China. Based on rbcL sequences the Indian specimen was positioned in a major clade with high support (>95 bootstrap and 0.95 posterior probability) containing two other species: T. okadae from Japan and T. hispida from the continental USA, Hawaii, the UK, and China. The divergences among these sequences were T. indica vs. T. okadae (2.8%) and T. indica vs. T. hispida (2.9-3.4%). The comparison of morphological characters of Thorea from India was not conclusive due to the inadequate descriptions in previous reports: most specimens reported as T. hispida fit within the circumscription of T. indica as described here. The previous report of T. siamensis from the Sai River is incorrect and the specimens fit within our description of T. indica. Thorea indica and T. okadae can be distinguished by minor morphometric characters and sexuality (dioecious vs. monoecious).