• 대한본초학회지
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• 제37권5호
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• pp.9-16
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• 2022

Objectives : The purpose of this study is to report that applying the genetic discrimination method to Pinelliae Tuber is suitable as a countermeasure for the limitations of morphological identification announced publicly in the Ministry of Food and Drug Safety(MFDS). Methods : Randomly selected fifty samples in Pinelliae Tuber imported from China were used for morphological and genetic identification. The morphological identification was applied method announced publicly by the MFDS. The traits of morphological identification were classified as Pinellia ternata, P. tripartita, Pinellia pedatisecta, and Typhonium flagelliforme, according to the formation of tuberous root and tuber morphology. The genetic identifications were conducted by Sequence Characterized Amplified Region(SCAR) marker and DNA barcoding analysis for cross-validation, respectively. SCAR marker was verified according to the presence or absence of amplicon through PCR amplification using species-specific primers. DNA barcoding analysis used sequence information of the matK region. Results : As a result of the morphological identification, 27 out of 50 samples were identified as original species 'P. ternata' of genuine 'Pinelliae Tuber', and 23 were identified as adulterant species 'P. pedatisecta'. Unlike this, the genetic identification was identified as the original species 'P. ternata' in all 50 samples in the SCAR marker and matK regional sequence analysis. Conclusions : Pinelliae Tuber of morphological mutant that can not be classified by morphological identification is imported from China. The SCAR marker would be used as accurate and efficient assays for species identification of the morphological mutant.

• Asian-Australasian Journal of Animal Sciences
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• 제27권2호
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• pp.194-200
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• 2014

This study investigated the chemical composition and ruminal degradability of corn stover in three maize-planting regions in Qiqihaer, Heilongjiang Province, China. The whole stover was separated into seven morphological fractions, i.e., leaf blade, leaf sheath, stem rind, stem pith, stem node, ear husk, and corn tassel. The assessment of nutritive value of corn stover and its fractions was performed based on laboratory assays of the morphological proportions, chemical composition, and in situ degradability of dry matter (DM), neutral detergent fiber (NDF), and acid detergent fiber (ADF). The chemical composition of corn stover was significantly different from plant top to bottom (p<0.05). Among the whole corn stover and seven morphological fractions, leaf blade had the highest crude protein (CP) content and the lowest NDF and ADF contents (p<0.05), whereas stem rind had the lowest CP content and the highest ADF and acid detergent lignin (ADL) contents (p<0.05). Ear husk had significantly higher NDF content and relatively lower ADL content than other corn stover fractions. Overall, the effective degradability of DM, NDF, and ADF in rumen was the highest in leaf blade and stem pith, followed by ear husk. The results indicate that leaf blade, ear husk, and stem pith potentially have higher nutritive values than the other fractions of corn stover. This study provides reference data for high-efficiency use of corn stover in feeding ruminants.

• 한국연초학회지
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• 제9권2호
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• pp.49-57
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• 1987

This study had been conducted to determine morphological characteristics of soil in the major districts cultivating flue-cured tobacco plant. Also native soil productivities were measured by means of bioassay planting tobacco plant without fertilizer at 87 selected soils through field experiment. Morphological characteristics of troll affecting the dry weight of tobacco leaves cultivated in the field were investigated Among soil morphological characteristics, topograpy, slope, topsoil depth, soil depth, and soil texture had influenced with high significant on the dry weight of tobacco leaves. For prediction of dry weight (productivity) of tobacco leaves without fertilizer , multiple regression analysis were introduced using soul morphological characteristics. A combination of topography, slope, topsoil depth, and soil texture was very reliable for prediction of productivity. The regression equation was y = -16.88 -14, 34$X_1$ +20.43$X_2$ +50.21$X_3$ -7.54$X_4$ +13.45$X_5$ R = $0.670^{**}$ Where $X_1$ : Topography $X_2$ : Slope $X_3$ : Topsoil Depth $X_4$ : Soil Depth $X_5$ : Soil Texture

• 로봇학회논문지
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• 제15권2호
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• pp.177-183
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• 2020

Collaborative Robot (Cobot) that can collaborate with humans by fusion with many advanced technologies among industrial robots in the industrial field are attracting attention. In this study, the engineers of Small and Medium Enterprises can directly participate in the cobot design, and ultimately, the possibility of deriving the shape design of the differentiated cobot was studied. The method applied to derive the shape design of differentiated cobot is 'Morphological Analysis'. First, the design elements of the form of cobots were derived as 'Link' and 'Joint'. In addition, by analyzing the image form of the Link and Joint of the existing cobot, a new form element of the Link and Joint was proposed. In order to quantitatively identify the most discriminating cobot shape design, FGI (Focus Group Interview) was conducted to derive image types of 4 Link and 3 Joint. Then, the most important 'Shape Combination' was carried out in morphological analysis, and 12 new cobot shape designs were drawn. Through this, the applicability of the morphological analysis method in the derivation of differentiated cobot shape design was examined.

• 교육시설 논문지
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• 제19권3호
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• pp.13-20
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• 2012

In this study, we performed the psychological analysis on campus facilities for university students with the observation that the evaluation on the physical environment of the campus is related to the image evaluation of the campus users. Specifically, we investigated the cognitive structure and the image evaluation structure of users, i.e., students, on the architectural and morphological elements of campus buildings. The investigation results are summarized as follows. 1) The cognitive structure of students on the architectural and morphological elements was different from building to building. In the case of architectural elements, the campus buildings were rated high, if they were designed with emphasis on symbolic elements, while the campus buildings with simple design were rated low. However, the morphological elements exhibited the opposite results. 2) We extracted six factors by performing the factor analysis for image evaluation. From the analysis results, we found that students showed a different structure of image evaluation for each building. In addition, the image evaluation structures were found to be related with the cognitive structures on architectural and morphological elements. 3) We also performed the correlation analysis between image evaluation structure and cognitive structure on architectural and morphological aspects. The analysis results indicated that each building showed different correlation patterns. In addition, the overall image became better, as they appraised "clarity" and "uniformity" more highly.

• Animal Systematics, Evolution and Diversity
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• 제15권1호
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• pp.11-25
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• 1999

가시톡토기 과에서 분류학적으로 문제되는 2아속 3종의 유연관계를 밝히기 위해 형태형질 및 효소유전자분석을 실시하였다. 형태형질분석과 효소의 분석에서 서로 다른 계통수가 만들어졌다. 형태형질분석에서는 아속간에 분리가 이루어지지 않았으나 효소분석의 경우 아속간에 확실히 구분되었으며 유전적 차이도 매우 컸다. 그러나 이들 계통수들은 distance method나 분지분석방법에서 모두 같은 분지순서를 나타냈는데 특히 형태형질분석의 경우에 형질평가방법과 형질비평가 방법을 사용했음에도 불구하고 결과는 같았다. 본 연구 결과 어떤 중요한 형태적인 형질이 효소분석에서 처럼 유전적 분화를 나타내면 강렬한 분류형질이 될 수 있다고 생각된다.

• 원예과학기술지
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• 제29권3호
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• pp.240-246
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• 2011

The principal objective of this study was to estimate the availability of morphological data on the basis of the guidelines of the International Union for the Protection of New Varieties of Plants (UPOV) with regard to the identification of the rose germplasm. The correlation of morphological traits and random amplified polymorphic DNA (RAPD) marker data among 44 rose cultivars was assessed via a mantel test. Thirty eight phenotypes were employed for morphological analysis. Sixteen primers were utilized for RAPD analysis, and these generated 225 polymorphic bands. The dendrogram based on the RAPD markersgrouped 44 rose cultivars according to their horticultural types. No significant correlation was observed between the morphological and RAPD marker data. We concluded that current UPOV traits could not be applied to study genetic variation. Further studies on morphological traits are required for the analysis of genetic variation among cultivars.

• 식물분류학회지
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• 제44권3호
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• pp.191-201
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• 2014

동아시아산 황기속의 외부형태학적 특징과 과피, 종피의 해부구조 및 표면 형태를 조사하여 분류학적으로 유용한 형질을 찾고자 하였다. 연구 결과, 황기속의 외부형태, 과피, 종피의 형태 및 해부학적 특징 중 생활사, 소엽의 형태, 꽃차례, 꽃잎의 색, 종자의 색, 과피의 형태, 과피 표면에서 털의 존재유무, 봉선의 구조, 그리고 내과피 층의 두께가 종을 나누는 식별형질로 사용될 수 있음을 확인하였다. 이를 바탕으로 중국에서 황기라는 약재로 사용되어지고 있는 4종의 형태 및 해부학적인 특성을 비교한 결과, 서로 같은 군으로 유집되었다. 한편, 분류학적으로 논쟁이 되고 있는 정선황기와 갯황기에서는 두 분류군간에 형태적 차이점을 발견 할 수 없었다. 따라서 금후 정선황기, 갯황기, A. bhotanensis의 종간 분류학적 처리를 명확히 규명하기 위하여 염기서열 및 분자세포학 수준에서의 연구가 필요하다.

• ALGAE
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• 제23권1호
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• pp.15-29
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• 2008

Many species in Gymnodiniales, which are unarmored dinoflagellates, are responsible for marine algal blooms and some of them have potent toxin in the cell. Their taxonomy has so far been well-defined, and several genera (e.g. Akashiwo, Gymnodinium, Karenia) have recently been re-described. In Korea, few works have been carried out on their taxonomical and molecular studies. This study focused on comparison of both morphological and molecular characteristics of five unarmored dinoflagellates on Korean coastal water: Akashiwo sanguinea, Cochlodinium polykrikoides, Gymnodinium catenatum, Gymnodinium impudicum and Karenia aureolum (=K. mikimotoi). Morphological characteristics observed here was in good accordance with the original descriptions of individual species. In addition, none of difference was found in morphological comparisons between the Korean and foreign strains. Furthermore, molecular analysis showed that the SSU rDNA sequences were generally identical according to each species. In some distinct features, A. sanguinea, which has generally the same morphological features, were divided into two groups: one was Korean isolates including European isolates, the other was American isolates. In the two groups, the nucleus was positioned differently: middle of the cells in the Korean isolates (GnSg02, GnSg03), near the epicone in American isolates (CCMP1593, CCMP1837). In addition, this was strongly supported by phylogenetic analysis, inferred from the SSU rDNA sequences. K. aureolum (GrAr01) was corresponded to European G. aureolum (=K. mikimotoi) in shape and position of nucleus, chloroplast, however, which is similar to K. digitata in view of having a finger-like sulcus. This was in good agreement with phylogenetic study of these species. G. catenatum have identical morphology except the ridge location, and their genotype of SSU rDNA was also identical to GenBank data of the same species. From this study, we found that the five Korean unarmored dinoflagellates are identical morphological characteristics and genotype to each species of foreign isolates.

• Animal Bioscience
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• 제36권2호
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• pp.191-199
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• 2023

Objective: This study aimed to investigate the significant single nucleotide polymorphisms (SNPs) and genes associated with nine reproduction and morphological traits in three breed populations of Chinese goats. Methods: The genome-wide association of nine reproduction and morphological traits (litter size, nipple number, wattle, skin color, coat color, black dorsal line, beard, beard length, and hind leg hair) were analyzed in three Chinese native goat breeds (n = 336) using an Illumina Goat SNP50 Beadchip. Results: A total of 17 genome-wide or chromosome-wide significant SNPs associated with one reproduction trait (litter size) and six morphological traits (wattle, coat color, black dorsal line, beard, beard length, and hind leg hair) were identified in three Chinese native goat breeds, and the candidate genes were annotated. The significant SNPs and corresponding putative candidate genes for each trait are as follows: two SNPs located on chromosomes 6 (CSN3) and 24 (TCF4) for litter size trait; two SNPs located on chromosome 9 (KATNA1) and 1 (UBASH3A) for wattle trait; three SNPs located on chromosome 26 (SORCS3), 24 (DYM), and 20 (PDE4D) for coat color trait; two SNPs located on chromosome 18 (TCF25) and 15 (CLMP) for black dorsal line trait; four SNPs located on chromosome 8, 2 (PAX3), 5 (PIK3C2G), and 28 (PLA2G12B and OIT3) for beard trait; one SNP located on chromosome 18 (KCNG4) for beard length trait; three SNPs located on chromosome 17 (GLRB and GRIA2), 28 (PGBD5), and 4 for hind leg hair trait. In contrast, there were no SNPs identified for nipple number and skin color. Conclusion: The significant SNPs or genes identified in this study provided novel insights into the genetic mechanism underlying important reproduction and morphological traits of three local goat breeds in Southern China as well as further potential applications for breeding goats.