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http://dx.doi.org/10.6116/kjh.2022.37.5.9.

A Case Report of Imports Morphological Variation of Pinelliae Tuber Based on the Genetic Analysis  

Kim, Wook Jin (한국한의학연구원 한약자원연구센터)
Choi, Goya (한국한의학연구원 한약자원연구센터)
Noh, Sumin (한국한의학연구원 한약자원연구센터)
Moon, Byeong Cheol (한국한의학연구원 한약자원연구센터)
Publication Information
The Korea Journal of Herbology / v.37, no.5, 2022 , pp. 9-16 More about this Journal
Abstract
Objectives : The purpose of this study is to report that applying the genetic discrimination method to Pinelliae Tuber is suitable as a countermeasure for the limitations of morphological identification announced publicly in the Ministry of Food and Drug Safety(MFDS). Methods : Randomly selected fifty samples in Pinelliae Tuber imported from China were used for morphological and genetic identification. The morphological identification was applied method announced publicly by the MFDS. The traits of morphological identification were classified as Pinellia ternata, P. tripartita, Pinellia pedatisecta, and Typhonium flagelliforme, according to the formation of tuberous root and tuber morphology. The genetic identifications were conducted by Sequence Characterized Amplified Region(SCAR) marker and DNA barcoding analysis for cross-validation, respectively. SCAR marker was verified according to the presence or absence of amplicon through PCR amplification using species-specific primers. DNA barcoding analysis used sequence information of the matK region. Results : As a result of the morphological identification, 27 out of 50 samples were identified as original species 'P. ternata' of genuine 'Pinelliae Tuber', and 23 were identified as adulterant species 'P. pedatisecta'. Unlike this, the genetic identification was identified as the original species 'P. ternata' in all 50 samples in the SCAR marker and matK regional sequence analysis. Conclusions : Pinelliae Tuber of morphological mutant that can not be classified by morphological identification is imported from China. The SCAR marker would be used as accurate and efficient assays for species identification of the morphological mutant.
Keywords
Pinellia ternata; Pinelliae Tuber; Morphology; SCAR marker; DNA barcoding analysis;
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1 Noh P, Kim WJ, Yang S, Choi G, Moon BC. PCR-based rapid diagnostic tools for the authentication of medicinal mistletoe species. Phytomedicine. 2021 ; 91 : 153667.   DOI
2 Kim WJ, Moon BC, Yang S, Han KS, Choi G, Lee AY. Rapid authentication of the herbal medicine plant species Aralia continentalis Kitag. and Angelica biserrata CQ Yuan and RH Shan using ITS2 sequences and multiplex-SCAR markers. Molecules. 2016 ; 21(3) : 270.   DOI
3 Kim WJ, Yang S, Choi G., Park I, Noh P, Seo CS, Moon BC. Development of conventional PCR and real-time PCR assays to discriminate the origins of Chinese pepper oil and herbal materials from Zanthoxylum. Journal of the Science of Food and Agriculture. 2019 ; 99(4) : 2021-2029.   DOI
4 Gao Z, Liu Y, Wang X, Wei X, Han J. DNA mini-barcoding: a derived barcoding method for herbal molecular identification. Frontiers in plant science. 2019 ; 10: 987.   DOI
5 Korea Institute of Oriental Medicine. Korean Medicinal Materials. Daejeon : Korea Institute of Oriental Medicine. 2015 : 102-109.
6 Jarvinen P, Palme A, Morales LO, Lannenpaa M, Keinanen M, Sopanen T, Lascoux M. Phylogenetic relationships of Betula species (Betulaceae) based on nuclear adh and chloroplast matK sequences. American Journal of Botany. 2004 ; 91(11) : 1834-45.   DOI
7 Kang Y, Choi J, Lee K, Moon B, Yoon A. New insight of various methods for in vitro propagation of Pinellia ternata (Thunb.) makinoas Korean traditional herbal medicine (Pinellia Tuber). Korean Herbal Medicine Informatics. 2016 ; 4(2) : 43-52.
8 Liu Y, Liang Z, Zhang Y. Induction and in vitro alkaloid yield of calluses and protocorm-like bodies (PLBs) from Pinellia ternata. In Vitro Cellular & Developmental Biology-Plant. 2010 ; 46(3) ; 239-245.   DOI
9 Ministry of Food and Drug Safety. The Korean Pharmacopoeia (electronic version). Published on the Internet; https://nedrug.mfds.go.kr/ekphome (accessed 2022-08-01).
10 Kim WJ, Yang S, Choi G, Moon BC. Identification of marker nucleotides for the molecular authentication of Araliae Continentalis Radix based on the analysis of universal DNA barcode, matK and rbcL, Sequences. The Korea Journal of Herbology. 2016 ; 31(5) : 15-23.   DOI
11 Ministry of Food and Drug Safety, National Institute of Food and Drug Safety Evaluation. The dispensatory on the visual and organoleptic examination of herbal medicine. Cheongju : Jinhan M&B. 2022 : 263-264.
12 Korea Institute of Oriental Medicine. Defining Dictionary for Medicinal Herbs 'Hanyak Giwon Sajeon'. 2021; Published on the Internet; https://oasis.kiom.re.kr/herbl ib/hminfo/hbmcod/hbmcodList.do (accessed 2022-04-25).
13 Moon BC, Kim WJ, Ji Y, Lee YM, Kang YM, Choi G. Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences. Genetics and Molecular Research. 2016 ; 15 : 1.
14 Choi YS, Kil KJ, Lee YJ. A Study on a morphological identification of Pinelliae Tuber, Typhonii Flagelliformis Rhizoma and Arisaematis Rhizoma. The Korea Journal of Herbology. 2007 ; 22(2) : 103-108.
15 Lee YM, Moon BC, Ji Y, Kim WJ, Kim HK. Molecular authentication of Pinelliae Tuber from its adulterants by the analysis of DNA barcodes, matK and rbcL genes. The Korea Journal of Herbology. 2013 ; 28(6) : 53-58.   DOI
16 Lee YM, Ji Y, Kang YM, Kim WJ, Choi G, Moon BC. Molecular authentication of Pinelliae Tuber and its common adulterants using RAPD-derived multiplex sequence characterized amplified region (multiplex-SCAR) markers. International Journal of Clinical and Experimental Medicine. 2016 ; 9(1) : 40-50.