• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.228-234
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• 2002

Bacillus subtilis CAU131 (KCCM 10257) isolated from a fermented shrimp product produces subtilein, tentatively named as a bacteriocin, which exhibited a bactericidal effect against closely related species such as Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 11778, and several other strains of Bacillus sp. The purification of the subtilein was achieved by applying a mono-Q anion exchange chromatography on FPLC and $C_18$ reverse-phase chromatography on HPLC. After purification, specific activity of subtilein was increased about 3,000-fold compared with culture broth and its molecular mass was about 5,000 Da on SDS-PAGE. The antimicrobial activity of subtilein was well maintained at acidic and neutral pHs between 3 and 8. Subtilein was relatively heat stable, and its antimicrobial activity remained for 2 h at $80^{\circ}C$. However, the activity was reduced after heating at $100^{\circ}C$, and about $80\%$ of the activity was found after 1 h incubation at $100^{\circ}C$. The treatment of Bacillus subtilis ATCC 6633 with subtilein led to morphological changes in stationary-phase cells and most cells appeared to be lysed.

• 한방안이비인후피부과학회지
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• 제15권2호
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• pp.20-32
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• 2002

The aim of this study was to investigate the effect of Codonopsis lanceolatae on the melanogenesis of HM3KO human melanoma cells biologically. The cells were treated for 3 days and 5 days with Codonopsis lanceolatae at several concentrations. The effects on tyrosinase activity and melanin contents were examined. And DOPAchrome tautomerase(TRP-2) activity was also examined to search a pathway influencing this inhibitory effect. 1. It was examined mushroom tyrosinase activity was significantly inhibited by Codonopsis lanceolatae. 2. Treatment with Codonopsis lanceolatae did not affect cell viability at the highest concentration, 1 ㎎／ml, and suppressed melanin contents as a time and dose dependent manner. 3. It was investigated treatment with Codonopsis lanceolatae inhibited tyrosinase, a key enzyme forming melanin, activity in a dose-dependent manner. 4. Treatment with Codonopsis lanceolatae did not affect DOPAchrome tautomerase(TRP-2) activity. 5. There was not a morphological change by Codonopsis lanceolatae microscopically. These results suggest that Codonopsis lanceolatae is a candidate for an efficient whitening agent which supresses melanogenesis by a Raper-Mason pathway.

• Journal of the Korean Wood Science and Technology
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• 제44권1호
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• pp.113-123
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• 2016

본 연구는 전나무 정유의 Epidermophyton floccosum, Trichophyton mentagrophytes, Trichophyton rubrum과 같은 피부사상균에 대한 항진균 활성을 구명하고 피부염 치료제로서의 전나무 정유의 잠재력을 평가하고자 하였다. 전나무 정유와 분획물, 유효성분의 표준시약을 이용하여 한천희석법, paper disc 확산법, TEM을 이용한 세포 변화 관찰 등을 통해 항진균 활성을 평가하였고, 주성분은 GC-MS로 분석하였다. 유효성분들의 시너지효과는 체커보드법을 통해 평가하였다. 그 결과, 가장 높은 활성을 나타낸 물질은 ${\alpha}$-bisabolol을 함유하고 있는 분획 G4였으며 다른 분획 보다 뛰어나게 높은 활성을 나타냈다. 체커보드법을 통해 ${\alpha}$-bisabolol과 bornyl acetate를 병용시 뛰어난시너지 효과를 나타냈고 세포 변화 관찰을 통해 분획 G4에 노출된 E. floccosum과 T. rubrum 균사는 정상세포와 달리 세포막과 세포소기관이 파괴됨이 관찰되었다. 따라서 전나무 정유와 그 구성성분들은 높은 항진균 효과를 가지고 있기 때문에 피부염 치료제의 원료로 이용이 가능할 것이라 기대되며, 특히 ${\alpha}$-bisabolol의 우수한 항진균 활성에 주목할 필요가 있다고 사료된다.

• Archives of Pharmacal Research
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• 제21권3호
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• pp.348-352
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• 1998

Different bacteria wwere seperated from saliva and teeth of cariogenic patients and identified by a variety of morphological and biochemical tests. Extracts of green tea strongly inhibited Escherichia coli, Streptococcus salivarius and Streptococcus mutans. The antibacterial effect of green and black tea extracts were compared with those of amoxicillin, cephradine and eugenol.

• 미생물학회지
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• 제15권2호
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• pp.93-99
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• 1977

Among the Streptomyces isolated from soil, two strains which have antibacterial activity aginst various pathogenic bacteria are identified as Streptomyces globosus and Streptomyces albus subsp. according to I.S.P. methods and Bergy's Manual of Determinative Bacteriology. Morphological and physiological characteristics of them on several media were observed. Antibiotics from S. albus subsp. or S. globosus was identified asw tetracycline or streptomycin-like substances respectively by the paper chromatographic behavior in eight solvent systems of V. Betina. And it was revealed that these antibiotic substances are stable to temperature and weak acid.

• Bulletin of the Korean Chemical Society
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• 제32권10호
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• pp.3793-3795
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• 2011

• Toxicological Research
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• 제14권4호
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• pp.489-494
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• 1998

In the present study, we have evaluated cytotoxic effects of Taraxaci Herba extract on human skin melanoma cells. The light microscopic study showed morphological changes AG-NOR (argyrophylic nucleolar organizer region) by silver chloride stain, and glycoprotein by PAS reaction of the treated cells. Disruptions in cell organelles were determined by SRB assay.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1411-1416
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• 2004

The objective of this work was to isolate a microorganism, able to produce high lactate dehydrogenase (LDH) activity, for use as a microbial feed additive. The LDH is an important enzyme for lactate conversion in the rumen, thereby possibly overcoming lactic acidosis owing to sudden increases of cereal in the diets of ruminants. In the present study, various bacterial strains were screened from a variety of environments. Among the isolated microorganisms, strain FFy 111-1 isolated from a Korean traditional fermented vegetable food called Kimchi showed the highest enzyme activity, along with retaining strong enzyme activity even in rumen fluid in vitro. Based on morphological and biochemical characteristics as well as compositions of cellular fatty acids plus API analyses, this strain was identified as Lactobacillus sp. The optimum temperature and pH for growth were found to be 30$^{\circ}C$ and pH 6.5, respectively. A maximum cell growth of 2.2 at $A_{650}$ together with LDH activity of 2.08 U per mL was achieved after 24 h of incubation. Initial characterization of FFy 111-1 suggested that it could be a potential candidate for use as a direct-fed microbial in the ruminant animals.

• Journal of Nutrition and Health
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• 제40권2호
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• pp.147-153
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• 2007

In this study, we investigated the anticancer activity of the fin of Thunnus Thynnus (TT). TT was extracted with methanol (TTM), and then further fractionated into four subfractions by using solvent partition method, affording hexane (TTMH), methanol (TTMM), butanol (TTMB) and aquous (TTMA) soluble fractions. We determined the cytotoxicity of these four fractions in four kind of cancer cell lines, such as HepG2, MCF-7, B16-F10 and HT29 by MTT assay. The TTMM showed the strongest cytotoxic effect at the concentration of 150 ${\mu}g/mL$, displaying 95% on the HepG2 cell lines and 82% on MCF-7 cell line. The morphological changes such as membrane shirinking and blebbing of cells were also observed by TTMM treatment in HT29 cell. In addition, we observed that quinone reductase (QR) activity was elevated by only TTMM and TTMH treatments in HepG2 cell. QR activity was increased to around 2.0 and 1.8 times in TTMM and TTMH treated HepG2 cell at 100 ${\mu}g/mL$, respectively, compared to that in control. Although further studies are needed, the present work could suggest that the fin of TT has a potential to be usable as a chemopreventive agent against cancer.

• 한국환경성돌연변이발암원학회지
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• 제18권1호
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• pp.37-42
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• 1998

In the present study, we have evaluated cytotoxic effects of the chloroform soluble fraction of the methanolic extract of Perilla frutescens in human oral epitheloid carcinoma cells. The light microscopic study showed morphological changes of the treated cells. Cell membrane damaging activity was measured by the lactate dehydrogenase (LDH) assay and disruptions in cell organelles were determined by 3-(4,5-dime-thylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), neutral red (NR) and sulforhodamine protein B (SRB) of colorimetric assay. These results suggest that Perilla frutescens retains a potential antitumor activity.