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http://dx.doi.org/10.5658/WOOD.2016.44.1.113

Evaluation on Anti-fungal Activity and Synergy Effects of Essential Oil and Their Constituents from Abies holophylla  

Kim, Seon-Hong (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Lee, Su-Yeon (Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, Korea Forest Research Institute)
Cho, Seong-Min (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Hong, Chang-Young (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Park, Mi-Jin (Division of Wood Chemistry & Microbiology, Department of Forest Resources Utilization, Korea Forest Research Institute)
Choi, In-Gyu (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
Publication Information
Journal of the Korean Wood Science and Technology / v.44, no.1, 2016 , pp. 113-123 More about this Journal
Abstract
This study was to investigate the antifungal activity of A. holophylla essential oil against dermatophytes, such as Epidermophyton floccosum, Trichophyton mentagrophytes and Trichophyton rubrum, and to determine the potential effective compound as dermatitis treatment. To evaluate the potential antifungal activities of A. holophylla essential oil and its fractions, paper disc diffusion and agar dilution method tested with morphological observation. Also, their major constituents were analyzed by GC/MS. To determine synergic effects of active ingredient from A. holophylla essential oil were carried out by checkerboard microtiter plate testing. The morphological changes of the dermatophytes exposed to active fraction G4 were observed by electron microscopes. As the results, the highest activities were identified in the fraction containing ${\alpha}$-bisabolol. A mixture of ${\alpha}$-bisabolol and bornyl acetate showed the synergy effects, expressing high potential effects. Also, morphological observation using electron microscopes showed a dramatic changes of cell membrane of E. floccosum and T. rubrum exposed to fraction G4 containing ${\alpha}$-bisabolol. In conclusion, A. holophylla essential oil and its constituents were expected to be used as antifungal agent or raw material for dermatitis therapy.
Keywords
Abies holophylla; dermatophytes; antifungal activity; essential oil; synergy effect;
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