• Analytical Science and Technology
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• v.19 no.5
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• pp.433-440
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• 2006

A HPLC/UV method was developed and validated for the rapid and simultaneous determination of urinary metabolites of organic solvents, mandelic acid, hippuric acid, phenylglyoxylic acid, ortho-, meta- and para-methylhippuric acid, using a monolithic column. The mobile phase was composed of tetrabutylammonium bromide as ion-pairing reagent with a flow rate of 2.4 mL/min. The total run time was less than 2.5 min for all six analytes. Good linearities were obtained for all the metabolites with correlation coefficients above 0.9993. Intra-day precision, accuracy and inter-day precision was 0.01~7.32%, 83.9~116.3% and 0.01~7.16%, respectively. The method was validated and confirmed by quantification of the quality assurance samples of Industrial Safety and Health Research Institute, Korea Occupational Safety and Health Agency.

• Analytical Science and Technology
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• v.20 no.4
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• pp.323-330
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• 2007

A simple and rapid HPLC method with fluorescence detection(FLD) for quantitation of penciclovir in human plasma using a monolithic column was developed and validated. Penciclovir and ganciclovir(internal standard, I.S.) were separated on a Chromolith column RP-18e ($4.6{\times}100mm$) with a mobile phase consisting of a mixture of (A) methanol/50 mM sodium phosphate buffer containing 200 mg/L sodium dodecyl sulfate (3/97, pH 2.5) and (B) methanol/50 mM sodium phosphate buffer containing 200 mg/L sodium dodecyl sulfate (50/50, pH 2.5) at a flow gradient of $1.6{\sim}4.0mL/min$. The retention times of penciclovir and internal standard were less than 4.0 min. Calibration curve was linear ($R^2=0.9994$) over a concentration range of $0.1{\sim}5{\mu}g/mL$. Intra-day precision, accuracy and inter-day precision were 1.36~8.55 %, 92.8~100.0 % and 0.93~5.62 %, respectively with a limit of quantitation at $0.1{\mu}g/mL$. The present HPLC-FLD method is sensitive, precise and accurate. The method described herein has been successfully used for the bioequivalence study of a famciclovir formulation product after oral administration to healthy Korean volunteers.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.357-363
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• 2006

Monolithic molecularly imprinted columns were designed and prepared by an in-situ thermal-initiated copolymerization technique for rapid separation of tryptophan and N- CBZ-phenylalanine enantiomers. The influence of polymerization conditions and separation conditions on the specific molecular recognition ability for enantiomers and diastereomers was investigated. The specious molecular recognition was found to be dependent on the stereo structures and the arrangement of functional groups of the imprinted molecule and the cavities in the molecularly imprinted polymer (MIP). Moreover, hydrogen bonding interactions and hydrophobic interactions played an important role in the retention and separation. Compared to conventional MIP preparation procedures, the present method is very simple, and its macroporous structure has excellent separation properties.

• Proceedings of the Korea Concrete Institute Conference
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• 1995.10a
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• pp.317-322
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• 1995

This study is to examine the usefulness in using precast girder-infilled steel tube column in reinforced concrete structures through the analysis of the test results, in order to develope the new composite structural system using precast girder-Infilled steel tube column, The variables of specimen are strength of concrete, the numble of hoops, the form of beam-column The variables of specimen are strength of concrete, the number of hoops, the form of beam-column joints. By raising strength of concrete and incresing number of hoops in beam-column joint, it becomes clear to take similar structure capacity to monolithic structures.

• Advances in concrete construction
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• v.9 no.3
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• pp.235-248
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• 2020

Progressive collapse in a structure occurs when load bearing members are failed and the adjoining structural elements cannot resist the redistributed forces and fails subsequently, that leads to complete collapse of structure. Recently, construction using precast concrete technology is adopted increasingly because it offers many advantages like faster construction, less requirement of skilled labours at site, reduced formwork and scaffolding, massive production with reduced amount of construction waste, better quality and better surface finishing as compared to conventional reinforced concrete construction. Connections are the critical elements for any precast structure, because in past, major collapse of precast structure took place because of connection failure. In this study, behavior of four different precast wet connections with U shaped reinforcement bars provided at different locations is evaluated. Reduced 1/3^rd scale precast beam column assemblies having two span beam and three columns with removed middle column are constructed and examined by performing experiments. The response of precast connections is compared with monolithic connection, under column removal scenario. The connection region of test specimens are filled by cast-in-place micro concrete with and without polypropylene fibers. Performance of specimen is evaluated on the basis of ultimate load carrying capacity, maximum deflection at the location of removed middle column, crack formation and failure propagation. Further, Finite element (FE) analysis is carried out for validation of experimental studies and understanding the performance of structural components. Monolithic and precast beam column assemblies are modeled using non-linear Finite Element (FE) analysis based software ABAQUS. Actual experimental conditions are simulated using appropriate boundary and loading conditions. Finite Element simulation results in terms of load versus deflection are compared with that of experimental study. The nonlinear FE analysis results shows good agreement with experimental results.

• Journal of the Korea Concrete Institute
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• v.22 no.6
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• pp.741-751
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• 2010

An experimental study was performed to investigate the earthquake resistance of the beam-column connections as a part of a precast concrete moment-resisting frame that uses precast concrete U-shaped shells for the beams. Five full-scale precast concrete specimens and one conventional monolithic concrete specimen were tested under cyclic loading. The parameters for this test were the reinforcement ratio, stirrup spacing, and end-strengthening details of the precast beam shell. The test results showed that regardless of the test parameters, the precast concrete beam-column connections showed good load-carrying capacity and deformation capacity, which were comparable to those of conventional monolithic concrete specimen. However, at large deformations, the beam-column connections of the precast concrete specimens were subjected to severe strength degradation due to diagonal shear cracks and the bond-slip of re-bars at the joint region. For this reason, the energy dissipation capacity and stiffness of the precast concrete specimens were significantly less than those of the cast-in-place specimen.

• Analytical Science and Technology
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• v.37 no.4
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• pp.220-229
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• 2024

This study aims to developing a method for estimating pharmaceutical compounds within a monolith column using high-performance liquid chromatography (HPLC). The monolithic column was prepared using copolymerization of glycidyl methacrylate, co-ethylene dimethacrylate, and co-acrylic acid inside a borosilicate tube of specific dimensions a 60 mm borosilicate tube length with 1.5 mm and 3.5 mm inner and outer diameters, respectively. A UV Ultra violet source with a wavelength of 365 nm was used, and the polymerization process involved mixing glycidyl methacrylate, acrylic acid, ethylene dimethacrylate as a binder, and 2,2-dimethoxy-2-phenyl acetate phenone as an initiator in suitable solvents consisting of ethanol and 1-hexanol. The polymerization process formed the monolith column after 4 minutes, and subsequently, the epoxy groups were altered to diol groups using 0.2 M hydrochloric acid HCl, which were pumped through the column for 3 hours at a flow rate of 10 µL·min^-1. Various techniques, such as Scanning Electron Microscope SEM, Brunauer-Emmett-Teller BET, Fourier-transform infrared spectroscopy FT-IR and HNMR, were utilized to characterize and confirm the structure of the monolith. The prepared monolith was employed to estimate and identify the pharmaceutical compound of warfarin using high-performance liquid chromatography HPLC. The analytical curve of warfarin was linear in the range of 3 to 100 ㎍·mL^-1 with an r² value of 0.999. The detection and quantification limits were 0.932 and 2.788 ㎍·mL^-1, respectively. The molar absorptivity and Sandells sensitivity were 2.99138 × 10⁶ L·mol^-1·cm^-1 and 103.1 × 10^-3 ㎍·cm^-2, respectively.

• Proceedings of the Korea Concrete Institute Conference
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• 1995.04a
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• pp.339-344
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• 1995

In the design of ductile moment-resisting frames (DMRFs) following the strong column-weak beam dsign philosophy, it is desirable that the joint and column remain essentially elastic in order to insure proper energy dissipation and lateral stability of the structure. The joint has been identified as the "weak link" in DMRFs because any stiffness or strength deterioration in this region can lead to substantial drifts and the possibility of collapse due to P-delta effects. Moreover, the engineer is faced with the difficult task of detailing an element whose size is determined by the framing members, but which must resist a set of loads very different from those used in the design of the beams and columns. Four 2/3-scale beam-column-slab joint assemblies were designed according to existing code requirements of ACI 318-89, representing interior joints of DMRFs with reinforced high strength concrete. The influence on aseismic behavior of beam-column joints due to monolithic slab, has been investigated.estigated.

• Journal of the Korea Concrete Institute
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• v.26 no.4
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• pp.545-553
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• 2014

This study suggested an integrated classification method for generalized characteristics of PC beam-column connection according to connection details. Quantifying the failure mode of PC-beam column connection and characteristics of corresponding details, this study suggested to use deformation contribution of each element of beam-column assemblage. According to the expected failure mode of beam-column connection assemblage, PC beam-column connection can be classified into 'equivalent monolithic system' and 'jointed system'. In this study, four test specimens were tested for verification of detailed classification method of PC beam-column connections. Test was carried out with typical beam-column connection test method. Load was applied at the top of test specimen and end of beams were restrained by hinge. In order to verify the deformation contribution of each test specimen, 34-LVDTs were mounted on test specimen. According to test results, deformation contribution of each test specimen have different characteristics. Deformation characteristics of joint and other components which are quantified by test results, equivalent monolithic system can be classified into two categories. Strong connection have extremely small deformation contribution of joint and much larger deformation contribution was shown in flexural behavior of beam. The other type of beam-column connection is ductile connection which allows the larger deformation in joint area compared with strong connection.

• Steel and Composite Structures
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• v.20 no.6
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• pp.1259-1274
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• 2016

A precast composite column system has been developed in this study by utilizing multi interlocking spiral steel into a centrifugally-formed hollow-core precast (CHPC) column. The proposed hybrid column system can have enhanced performances in the composite interaction behavior between the hollowed precast column and cast-in-place (CIP) core-filled concrete, the lap splice performance of bundled bars, and the confining effect of concrete. In the experimental program, reversed cyclic loading tests were conducted on a conventional reinforced concrete (RC) column fabricated monolithically, two CHPC columns filled with CIP concrete, and two steel-reinforced concrete (SRC) columns. It was confirmed that the interlocking spirals was very effective to enhance the structural performance of the CHPC column, and all the hollow-core precast column specimens tested in this study showed good seismic performances comparable to the monolithic control specimen.