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http://dx.doi.org/10.5806/AST.2006.19.5.433

Rapid and simultaneous determination of metabolites of organic solvents in human urine by high-performance liquid chromatography using a monolithic column  

Han, Sang Beom (College of Pharmacy, Chung-Ang University)
Lee, Sang-Ju (College of Pharmacy, Chung-Ang University)
Lee, Cheol-Woo (College of Pharmacy, Chung-Ang University)
Yoon, Seo Hyun (College of Pharmacy, Chung-Ang University)
Joung, Sun Kyung (College of Pharmacy, Chung-Ang University)
Youm, Jeong-Rok (College of Pharmacy, Chung-Ang University)
Publication Information
Analytical Science and Technology / v.19, no.5, 2006 , pp. 433-440 More about this Journal
Abstract
A HPLC/UV method was developed and validated for the rapid and simultaneous determination of urinary metabolites of organic solvents, mandelic acid, hippuric acid, phenylglyoxylic acid, ortho-, meta- and para-methylhippuric acid, using a monolithic column. The mobile phase was composed of tetrabutylammonium bromide as ion-pairing reagent with a flow rate of 2.4 mL/min. The total run time was less than 2.5 min for all six analytes. Good linearities were obtained for all the metabolites with correlation coefficients above 0.9993. Intra-day precision, accuracy and inter-day precision was 0.01~7.32%, 83.9~116.3% and 0.01~7.16%, respectively. The method was validated and confirmed by quantification of the quality assurance samples of Industrial Safety and Health Research Institute, Korea Occupational Safety and Health Agency.
Keywords
monolithic column; urinary metabolites; organic solvents; HPLC;
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